YIL128W / MET18

Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19

Zygosity: Homozygous strain
fixedexpanded
Profile for YIL128W / MET18

Click on Significant Values for Screen Details ID:SGTC_133|Compound:0724-0033|FD-Score:-2.62|P-value:4.94E-4 ID:SGTC_289|Compound:k017-0060|FD-Score:-2.88|P-value:1.78E-4 ID:SGTC_535|Compound:2293-4129|FD-Score:-5.51|P-value:7.35E-11 ID:SGTC_989|Compound:1501-0895|FD-Score:-3.62|P-value:6.33E-6 ID:SGTC_1056|Compound:3448-1962|FD-Score:-2.45|P-value:9.54E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:-3.34|P-value:2.37E-5 ID:SGTC_1073|Compound:triclabendazole|FD-Score:-3.21|P-value:4.35E-5 ID:SGTC_1078|Compound:idebenone|FD-Score:-3.43|P-value:1.54E-5 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:-5.11|P-value:1.17E-9 ID:SGTC_1084|Compound:voriconazole|FD-Score:-2.71|P-value:3.48E-4 ID:SGTC_1120|Compound:ficusin|FD-Score:-2.69|P-value:3.86E-4 ID:SGTC_1123|Compound:mangostin|FD-Score:-3.70|P-value:4.15E-6 ID:SGTC_1126|Compound:isoteolin|FD-Score:-3.86|P-value:1.80E-6 ID:SGTC_1165|Compound:k072-0275|FD-Score:-5.42|P-value:1.38E-10 ID:SGTC_1167|Compound:k781-0610|FD-Score:-5.73|P-value:1.53E-11 ID:SGTC_1177|Compound:0983-0204|FD-Score:-2.51|P-value:7.49E-4 ID:SGTC_1184|Compound:1269-1718|FD-Score:-3.28|P-value:3.19E-5 ID:SGTC_1189|Compound:1431-2119|FD-Score:-6.61|P-value:1.51E-14 ID:SGTC_1194|Compound:1488-0194|FD-Score:-4.86|P-value:5.56E-9 ID:SGTC_1210|Compound:3013-0140|FD-Score:-3.89|P-value:1.56E-6 ID:SGTC_1372|Compound:2752-0194|FD-Score:-2.64|P-value:4.67E-4 ID:SGTC_1380|Compound:2291-0730|FD-Score:3.50|P-value:4.56E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:-3.92|P-value:1.33E-6 ID:SGTC_1453|Compound:4237-0541|FD-Score:-4.42|P-value:8.50E-8 ID:SGTC_1454|Compound:4239-0054|FD-Score:-3.84|P-value:2.03E-6 ID:SGTC_1475|Compound:4092-1048|FD-Score:-3.17|P-value:5.11E-5 ID:SGTC_1481|Compound:3346-2004|FD-Score:-2.52|P-value:7.31E-4 ID:SGTC_1490|Compound:4089-0339|FD-Score:-3.26|P-value:3.36E-5 ID:SGTC_1569|Compound:carminic acid|FD-Score:-4.08|P-value:5.75E-7 ID:SGTC_1748|Compound:st037455|FD-Score:3.44|P-value:5.94E-4 ID:SGTC_1751|Compound:st044839|FD-Score:-3.20|P-value:4.44E-5 ID:SGTC_1763|Compound:st044482|FD-Score:-2.78|P-value:2.70E-4 ID:SGTC_1920|Compound:st075728|FD-Score:-3.69|P-value:4.34E-6 ID:SGTC_2073|Compound:5212005|FD-Score:-2.94|P-value:1.38E-4 ID:SGTC_2310|Compound:7668677|FD-Score:3.55|P-value:3.79E-4 ID:SGTC_2314|Compound:7727897|FD-Score:-2.92|P-value:1.49E-4 ID:SGTC_2460|Compound:5552655|FD-Score:-2.58|P-value:5.87E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:3.32|P-value:9.19E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:3.34|P-value:8.54E-4 ID:SGTC_2767|Compound:sibutramine|FD-Score:3.42|P-value:6.36E-4 ID:SGTC_2783|Compound:7738165|FD-Score:-3.75|P-value:3.27E-6 ID:SGTC_2835|Compound:9006588|FD-Score:3.51|P-value:4.47E-4 ID:SGTC_3058|Compound:9093861|FD-Score:3.40|P-value:6.78E-4 ID:SGTC_3108|Compound:9121785|FD-Score:-3.42|P-value:1.65E-5 ID:SGTC_3217|Compound:9129673|FD-Score:-3.53|P-value:9.57E-6 ID:SGTC_3341|Compound:9147222|FD-Score:-6.15|P-value:6.06E-13 ID:SGTC_3345|Compound:9148410|FD-Score:-5.47|P-value:9.64E-11 ID:SGTC_133|Compound:0724-0033|FD-Score:-2.62|P-value:4.94E-4 ID:SGTC_289|Compound:k017-0060|FD-Score:-2.88|P-value:1.78E-4 ID:SGTC_535|Compound:2293-4129|FD-Score:-5.51|P-value:7.35E-11 ID:SGTC_989|Compound:1501-0895|FD-Score:-3.62|P-value:6.33E-6 ID:SGTC_1056|Compound:3448-1962|FD-Score:-2.45|P-value:9.54E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:-3.34|P-value:2.37E-5 ID:SGTC_1073|Compound:triclabendazole|FD-Score:-3.21|P-value:4.35E-5 ID:SGTC_1078|Compound:idebenone|FD-Score:-3.43|P-value:1.54E-5 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:-5.11|P-value:1.17E-9 ID:SGTC_1084|Compound:voriconazole|FD-Score:-2.71|P-value:3.48E-4 ID:SGTC_1120|Compound:ficusin|FD-Score:-2.69|P-value:3.86E-4 ID:SGTC_1123|Compound:mangostin|FD-Score:-3.70|P-value:4.15E-6 ID:SGTC_1126|Compound:isoteolin|FD-Score:-3.86|P-value:1.80E-6 ID:SGTC_1165|Compound:k072-0275|FD-Score:-5.42|P-value:1.38E-10 ID:SGTC_1167|Compound:k781-0610|FD-Score:-5.73|P-value:1.53E-11 ID:SGTC_1177|Compound:0983-0204|FD-Score:-2.51|P-value:7.49E-4 ID:SGTC_1184|Compound:1269-1718|FD-Score:-3.28|P-value:3.19E-5 ID:SGTC_1189|Compound:1431-2119|FD-Score:-6.61|P-value:1.51E-14 ID:SGTC_1194|Compound:1488-0194|FD-Score:-4.86|P-value:5.56E-9 ID:SGTC_1210|Compound:3013-0140|FD-Score:-3.89|P-value:1.56E-6 ID:SGTC_1372|Compound:2752-0194|FD-Score:-2.64|P-value:4.67E-4 ID:SGTC_1380|Compound:2291-0730|FD-Score:3.50|P-value:4.56E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:-3.92|P-value:1.33E-6 ID:SGTC_1453|Compound:4237-0541|FD-Score:-4.42|P-value:8.50E-8 ID:SGTC_1454|Compound:4239-0054|FD-Score:-3.84|P-value:2.03E-6 ID:SGTC_1475|Compound:4092-1048|FD-Score:-3.17|P-value:5.11E-5 ID:SGTC_1481|Compound:3346-2004|FD-Score:-2.52|P-value:7.31E-4 ID:SGTC_1490|Compound:4089-0339|FD-Score:-3.26|P-value:3.36E-5 ID:SGTC_1569|Compound:carminic acid|FD-Score:-4.08|P-value:5.75E-7 ID:SGTC_1748|Compound:st037455|FD-Score:3.44|P-value:5.94E-4 ID:SGTC_1751|Compound:st044839|FD-Score:-3.20|P-value:4.44E-5 ID:SGTC_1763|Compound:st044482|FD-Score:-2.78|P-value:2.70E-4 ID:SGTC_1920|Compound:st075728|FD-Score:-3.69|P-value:4.34E-6 ID:SGTC_2073|Compound:5212005|FD-Score:-2.94|P-value:1.38E-4 ID:SGTC_2310|Compound:7668677|FD-Score:3.55|P-value:3.79E-4 ID:SGTC_2314|Compound:7727897|FD-Score:-2.92|P-value:1.49E-4 ID:SGTC_2460|Compound:5552655|FD-Score:-2.58|P-value:5.87E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:3.32|P-value:9.19E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:3.34|P-value:8.54E-4 ID:SGTC_2767|Compound:sibutramine|FD-Score:3.42|P-value:6.36E-4 ID:SGTC_2783|Compound:7738165|FD-Score:-3.75|P-value:3.27E-6 ID:SGTC_2835|Compound:9006588|FD-Score:3.51|P-value:4.47E-4 ID:SGTC_3058|Compound:9093861|FD-Score:3.40|P-value:6.78E-4 ID:SGTC_3108|Compound:9121785|FD-Score:-3.42|P-value:1.65E-5 ID:SGTC_3217|Compound:9129673|FD-Score:-3.53|P-value:9.57E-6 ID:SGTC_3341|Compound:9147222|FD-Score:-6.15|P-value:6.06E-13 ID:SGTC_3345|Compound:9148410|FD-Score:-5.47|P-value:9.64E-11

Top fitness defect scores for YIL128W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2310 7668677 3.55 200.0 µM 3.79E-4 49 1.91E-4
2 SGTC_2835 9006588 3.51 71.4 µM 4.47E-4 61 2.25E-4
3 SGTC_1380 2291-0730 3.50 164.0 µM 4.56E-4 39 2.29E-4
4 SGTC_1748 st037455 3.44 53.6 µM 5.94E-4 51 2.96E-4
5 SGTC_2767 sibutramine 3.42 71.5 µM 6.36E-4 35 3.17E-4
6 SGTC_3058 9093861 3.40 49.5 µM 6.78E-4 37 3.37E-4
7 SGTC_2637 3-alpha-acetoxydihydrodeoxygedunin 3.34 100.0 µM 8.54E-4 43 4.22E-4
8 SGTC_2536 dehydroabietamide 3.32 32.0 µM 9.19E-4 63 4.54E-4
9 SGTC_3062 9077494 3.27 49.5 µM 0.00107 mitochondrial response to ROS 55 5.29E-4
10 SGTC_1734 st037168 3.24 69.4 µM 0.00120 53 5.91E-4
11 SGTC_2330 7979373 3.23 200.0 µM 0.00127 60S ribosome export 61 6.25E-4
12 SGTC_2276 7947513 3.16 42.1 µM 0.00159 50 7.77E-4
13 SGTC_943 3253-1379 3.16 105.0 µM 0.00160 53 7.84E-4
14 SGTC_1776 st049249 3.09 45.9 µM 0.00205 50 0.00100
15 SGTC_365 0121-0052 3.08 13.6 µM 0.00214 mitochondrial response to ROS 120 0.00104
16 SGTC_150 3970-0969 3.01 77.2 µM 0.00270 124 0.00131
17 SGTC_2528 xanthyletin 2.97 44.8 µM 0.00306 60 0.00148
18 SGTC_2764 methyldopa 2.97 94.7 µM 0.00310 74 0.00150
19 SGTC_100 0086-0128 2.95 49.6 µM 0.00329 copper-dependent oxidative stress 112 0.00159
20 SGTC_2572 astragaloside iv 2.93 100.0 µM 0.00346 80 0.00168

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.095 3.28E-8 YNL193W_p YNL193W_p hom Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis
0.080 3.88E-6 YKL015W PUT3 hom Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences as a dimer and undergoes a conformational change to form the active state; differentially phosphorylated in the presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain
0.078 6.04E-6 YOL014W_p YOL014W_p hom Putative protein of unknown function
0.076 9.63E-6 YNR050C LYS9 hom Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112
0.075 1.39E-5 YGR155W CYS4 hom Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria
0.075 1.53E-5 YJR020W_d YJR020W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.075 1.57E-5 YKL081W TEF4 hom Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex
0.067 9.56E-5 YPL025C_d YPL025C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.065 1.79E-4 YDL208W NHP2 het Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing
0.065 1.86E-4 YNL263C YIF1 het Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3
0.061 3.75E-4 YOL017W ESC8 hom Protein involved in telomeric and mating-type locus silencing; interacts with Sir2p and also interacts with Gal11p, which is a component of the RNA pol II mediator complex; ESC8 has a paralog, IOC3, that arose from the whole genome duplication
0.060 4.73E-4 YJL078C PRY3 hom Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p
0.059 5.75E-4 YDL231C BRE4 hom Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport
0.058 7.94E-4 YGL159W_p YGL159W_p hom Putative protein of unknown function; deletion mutant has no detectable phenotype
0.058 8.93E-4 YNL047C SLM2 hom Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; SLM2 has a paralog, SLM1, that arose from the whole genome duplication