YIL148W / RPL40A

Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YIL148W / RPL40A

Click on Significant Values for Screen Details ID:SGTC_57|Compound:miconazole|FD-Score:-5.40|P-value:4.27E-8 ID:SGTC_58|Compound:0957-0393|FD-Score:10.70|P-value:1.40E-26 ID:SGTC_73|Compound:0327-0325|FD-Score:-3.65|P-value:1.41E-4 ID:SGTC_114|Compound:0364-0001|FD-Score:3.98|P-value:4.48E-5 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.47|P-value:2.81E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:5.46|P-value:3.56E-8 ID:SGTC_168|Compound:3970-0862|FD-Score:3.85|P-value:7.49E-5 ID:SGTC_397|Compound:arbidol|FD-Score:-3.11|P-value:9.88E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.46|P-value:4.83E-6 ID:SGTC_629|Compound:3448-6466|FD-Score:3.16|P-value:9.33E-4 ID:SGTC_779|Compound:1360-0071|FD-Score:-3.37|P-value:4.04E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:3.57|P-value:2.23E-4 ID:SGTC_1179|Compound:1021-1431|FD-Score:5.11|P-value:2.37E-7 ID:SGTC_1308|Compound:1187-1617|FD-Score:4.85|P-value:8.73E-7 ID:SGTC_1442|Compound:4112-3290|FD-Score:3.21|P-value:7.80E-4 ID:SGTC_1458|Compound:k015-0034|FD-Score:3.30|P-value:5.74E-4 ID:SGTC_1729|Compound:st035615|FD-Score:3.50|P-value:2.86E-4 ID:SGTC_1731|Compound:st035964|FD-Score:3.67|P-value:1.51E-4 ID:SGTC_1735|Compound:st034310|FD-Score:-3.52|P-value:2.35E-4 ID:SGTC_1848|Compound:st056003|FD-Score:3.40|P-value:4.04E-4 ID:SGTC_1901|Compound:st060216|FD-Score:6.43|P-value:1.09E-10 ID:SGTC_1902|Compound:st060212|FD-Score:3.47|P-value:3.14E-4 ID:SGTC_1915|Compound:st067105|FD-Score:3.29|P-value:5.96E-4 ID:SGTC_1941|Compound:st076221|FD-Score:-3.20|P-value:7.39E-4 ID:SGTC_1948|Compound:st076329|FD-Score:-3.17|P-value:8.17E-4 ID:SGTC_2338|Compound:7999974|FD-Score:5.12|P-value:2.20E-7 ID:SGTC_2340|Compound:7987427|FD-Score:-3.45|P-value:2.99E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.24|P-value:7.21E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:3.86|P-value:7.25E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.40|P-value:4.09E-4 ID:SGTC_2809|Compound:7964133|FD-Score:4.71|P-value:1.72E-6 ID:SGTC_2920|Compound:7982904|FD-Score:-4.08|P-value:2.56E-5 ID:SGTC_2947|Compound:9072639|FD-Score:-4.26|P-value:1.19E-5 ID:SGTC_2952|Compound:9073731|FD-Score:-4.19|P-value:1.57E-5 ID:SGTC_3018|Compound:9082602|FD-Score:-3.20|P-value:7.41E-4 ID:SGTC_3188|Compound:9110789|FD-Score:3.61|P-value:1.85E-4 ID:SGTC_57|Compound:miconazole|FD-Score:-5.40|P-value:4.27E-8 ID:SGTC_58|Compound:0957-0393|FD-Score:10.70|P-value:1.40E-26 ID:SGTC_73|Compound:0327-0325|FD-Score:-3.65|P-value:1.41E-4 ID:SGTC_114|Compound:0364-0001|FD-Score:3.98|P-value:4.48E-5 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.47|P-value:2.81E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:5.46|P-value:3.56E-8 ID:SGTC_168|Compound:3970-0862|FD-Score:3.85|P-value:7.49E-5 ID:SGTC_397|Compound:arbidol|FD-Score:-3.11|P-value:9.88E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.46|P-value:4.83E-6 ID:SGTC_629|Compound:3448-6466|FD-Score:3.16|P-value:9.33E-4 ID:SGTC_779|Compound:1360-0071|FD-Score:-3.37|P-value:4.04E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:3.57|P-value:2.23E-4 ID:SGTC_1179|Compound:1021-1431|FD-Score:5.11|P-value:2.37E-7 ID:SGTC_1308|Compound:1187-1617|FD-Score:4.85|P-value:8.73E-7 ID:SGTC_1442|Compound:4112-3290|FD-Score:3.21|P-value:7.80E-4 ID:SGTC_1458|Compound:k015-0034|FD-Score:3.30|P-value:5.74E-4 ID:SGTC_1729|Compound:st035615|FD-Score:3.50|P-value:2.86E-4 ID:SGTC_1731|Compound:st035964|FD-Score:3.67|P-value:1.51E-4 ID:SGTC_1735|Compound:st034310|FD-Score:-3.52|P-value:2.35E-4 ID:SGTC_1848|Compound:st056003|FD-Score:3.40|P-value:4.04E-4 ID:SGTC_1901|Compound:st060216|FD-Score:6.43|P-value:1.09E-10 ID:SGTC_1902|Compound:st060212|FD-Score:3.47|P-value:3.14E-4 ID:SGTC_1915|Compound:st067105|FD-Score:3.29|P-value:5.96E-4 ID:SGTC_1941|Compound:st076221|FD-Score:-3.20|P-value:7.39E-4 ID:SGTC_1948|Compound:st076329|FD-Score:-3.17|P-value:8.17E-4 ID:SGTC_2338|Compound:7999974|FD-Score:5.12|P-value:2.20E-7 ID:SGTC_2340|Compound:7987427|FD-Score:-3.45|P-value:2.99E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.24|P-value:7.21E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:3.86|P-value:7.25E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.40|P-value:4.09E-4 ID:SGTC_2809|Compound:7964133|FD-Score:4.71|P-value:1.72E-6 ID:SGTC_2920|Compound:7982904|FD-Score:-4.08|P-value:2.56E-5 ID:SGTC_2947|Compound:9072639|FD-Score:-4.26|P-value:1.19E-5 ID:SGTC_2952|Compound:9073731|FD-Score:-4.19|P-value:1.57E-5 ID:SGTC_3018|Compound:9082602|FD-Score:-3.20|P-value:7.41E-4 ID:SGTC_3188|Compound:9110789|FD-Score:3.61|P-value:1.85E-4

Top fitness defect scores for YIL148W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_58 0957-0393 10.70 9.0 µM 1.40E-26 cell wall 70 3.46E-27
2 SGTC_1901 st060216 6.43 58.6 µM 1.09E-10 2 6.31E-11
3 SGTC_138 0850-0259 5.46 40.9 µM 3.56E-8 cell wall 126 2.35E-8
4 SGTC_2338 7999974 5.12 200.0 µM 2.20E-7 7 1.52E-7
5 SGTC_1179 1021-1431 5.11 9.3 µM 2.37E-7 17 1.64E-7
6 SGTC_1308 1187-1617 4.85 41.4 µM 8.73E-7 fatty acid desaturase (OLE1) 22 6.23E-7
7 SGTC_2809 7964133 4.71 13.0 µM 1.72E-6 60S ribosome export 18 1.25E-6
8 SGTC_114 0364-0001 3.98 490.3 µM 4.48E-5 48 3.51E-5
9 SGTC_2595 hexamethylquercetagetin 3.86 100.0 µM 7.25E-5 28 5.75E-5
10 SGTC_168 3970-0862 3.85 530.5 µM 7.49E-5 127 5.94E-5
11 SGTC_1731 st035964 3.67 63.0 µM 1.51E-4 39 1.22E-4
12 SGTC_3188 9110789 3.61 49.5 µM 1.85E-4 iron homeostasis 32 1.51E-4
13 SGTC_944 3370-0224 3.57 231.0 µM 2.23E-4 35 1.82E-4
14 SGTC_1729 st035615 3.50 46.0 µM 2.86E-4 51 2.35E-4
15 SGTC_1902 st060212 3.47 70.5 µM 3.14E-4 20 2.58E-4
16 SGTC_1848 st056003 3.40 16.4 µM 4.04E-4 33 3.34E-4
17 SGTC_2688 kanamycin b 3.40 96.7 µM 4.09E-4 calcium & mitochondrial duress 459 3.38E-4
18 SGTC_1458 k015-0034 3.30 6.3 µM 5.74E-4 29 4.79E-4
19 SGTC_1915 st067105 3.29 30.6 µM 5.96E-4 RNA pol III & RNase P/MRP 80 4.98E-4
20 SGTC_2408 sulbentine 3.24 18.4 µM 7.21E-4 81 6.05E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.291 2.66E-66 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.289 9.01E-66 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.282 2.05E-62 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.270 3.75E-57 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.267 6.85E-56 YKL115C_d YKL115C_d hom Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1
0.261 2.77E-53 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.257 1.19E-51 YBR292C_d YBR292C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene
0.252 1.27E-49 YLR150W STM1 hom Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
0.245 3.70E-47 YKL033W-A_p YKL033W-A_p hom Putative protein of unknown function; similar to uncharacterized proteins from other fungi
0.244 1.21E-46 YML016C PPZ1 hom Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance
0.238 2.53E-44 YLR443W ECM7 hom Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency
0.233 1.65E-42 YNL314W DAL82 hom Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
0.229 2.56E-41 YDL025C RTK1 hom Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress
0.229 4.34E-41 YBR120C CBP6 hom Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.229 5.08E-41 YPL269W KAR9 hom Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase