HIPHOP chemogenomics database

YJL027C_p

Putative protein of unknown function

Zygosity: Homozygous strain

fixedexpanded
Profile for YJL027C / YJL027C

Click on Significant Values for Screen Details

Top fitness defect scores for YJL027C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_273	hydroxyurea	32.70	18.1 mM	4.34E-249	heme biosynthesis & mitochondrial translocase	1	3.91E-234
2	SGTC_2770	toltrazuril	7.36	17.5 µM	1.36E-14		3	9.42E-14
3	SGTC_157	1348-1695	6.29	65.8 µM	3.61E-11	iron homeostasis	20	1.54E-10
4	SGTC_1227	3909-8010	6.23	46.6 µM	5.45E-11		2	2.27E-10
5	SGTC_604	1000-0114	5.74	314.0 µM	1.35E-9		2	4.61E-9
6	SGTC_472	calcifediol	5.54	10.0 µM	4.86E-9		1	1.53E-8
7	SGTC_2295	7971645	5.32	200.0 µM	1.83E-8		9	5.32E-8
8	SGTC_1221	2958-7315	5.07	257.0 µM	7.40E-8		6	1.97E-7
9	SGTC_624	k048-0052	4.68	28.0 µM	6.07E-7	Golgi	8	1.42E-6
10	SGTC_1186	1319-0216	4.35	26.0 µM	3.17E-6		12	6.70E-6
11	SGTC_2283	7944793	4.23	200.0 µM	5.81E-6		32	1.18E-5
12	SGTC_1678	st019265	4.10	80.2 µM	1.07E-5	60S ribosome export	16	2.10E-5
13	SGTC_462	ag-879	4.06	158.0 µM	1.26E-5		27	2.45E-5
14	SGTC_491	tetrandrine	3.93	80.3 µM	2.31E-5		12	4.32E-5
15	SGTC_1212	0520-0165	3.84	585.0 µM	3.41E-5		36	6.22E-5
16	SGTC_387	norethindrone acetate	3.83	66.7 µM	3.51E-5		18	6.40E-5
17	SGTC_372	0155-0182	3.79	15.9 µM	4.10E-5	mitochondrial response to ROS	32	7.41E-5
18	SGTC_381	3461-2219	3.57	42.7 µM	1.04E-4	copper-dependent oxidative stress	28	1.77E-4
19	SGTC_379	3970-0972	3.51	5.9 µM	1.33E-4	tubulin folding & SWR complex	51	2.23E-4
20	SGTC_1682	st012968	3.43	530.0 nM	1.82E-4		35	2.99E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.386	6.48E-120	YDR047W	HEM12	het	Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
0.181	4.12E-26	YDL064W	UBC9	het	SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC)
0.180	7.88E-26	YBR237W	PRP5	het	RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA
0.172	8.23E-24	YCR086W	CSM1	hom	Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation
0.171	2.37E-23	YHL013C	OTU2	hom	Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress
0.164	1.50E-21	YDR423C	CAD1	hom	AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication
0.163	1.80E-21	YPL207W	TYW1	hom	Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron
0.147	9.99E-18	YGR180C	RNR4	hom	Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.142	1.49E-16	YNL114C_d	YNL114C_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit
0.137	2.01E-15	YPL170W	DAP1	hom	Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis
0.136	2.76E-15	YDL101C	DUN1	hom	Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair
0.136	3.17E-15	YKR076W	ECM4	hom	Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.133	9.13E-15	YAL047C	SPC72	hom	Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization
0.129	5.61E-14	YDL163W_d	YDL163W_d	het	Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase
0.128	1.07E-13	YBR133C	HSL7	hom	Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress