YJL058C / BIT61

Subunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL058C / BIT61

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.96|P-value:1.10E-5 ID:SGTC_12|Compound:0849-0099|FD-Score:3.03|P-value:5.84E-4 ID:SGTC_66|Compound:0109-0187|FD-Score:-6.06|P-value:4.44E-11 ID:SGTC_506|Compound:n9-isopropylolomoucine|FD-Score:2.94|P-value:8.06E-4 ID:SGTC_562|Compound:3772-2733|FD-Score:2.98|P-value:6.92E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:3.85|P-value:1.83E-5 ID:SGTC_633|Compound:0437-0093|FD-Score:-3.78|P-value:2.62E-5 ID:SGTC_1013|Compound:4112-3890|FD-Score:3.30|P-value:2.03E-4 ID:SGTC_1066|Compound:ketoconazole|FD-Score:-3.69|P-value:4.01E-5 ID:SGTC_1085|Compound:doxorubicin|FD-Score:2.96|P-value:7.38E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.62|P-value:5.52E-5 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-3.08|P-value:4.98E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:-4.31|P-value:2.08E-6 ID:SGTC_1303|Compound:1160-0052|FD-Score:-3.64|P-value:5.07E-5 ID:SGTC_1367|Compound:1812-0510|FD-Score:2.89|P-value:9.78E-4 ID:SGTC_1741|Compound:st038134|FD-Score:4.66|P-value:2.98E-7 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.69|P-value:3.97E-5 ID:SGTC_2001|Compound:4023502|FD-Score:3.11|P-value:4.23E-4 ID:SGTC_2006|Compound:4011487|FD-Score:4.06|P-value:6.58E-6 ID:SGTC_2024|Compound:5144227|FD-Score:-4.36|P-value:1.60E-6 ID:SGTC_2052|Compound:5265182|FD-Score:-3.02|P-value:6.27E-4 ID:SGTC_2056|Compound:5235370|FD-Score:-3.81|P-value:2.29E-5 ID:SGTC_2396|Compound:5342950|FD-Score:-3.19|P-value:3.33E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-3.01|P-value:6.40E-4 ID:SGTC_2428|Compound:5615643|FD-Score:-3.22|P-value:2.95E-4 ID:SGTC_2432|Compound:5630864|FD-Score:-4.25|P-value:2.71E-6 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.11|P-value:4.19E-4 ID:SGTC_2570|Compound:licareol|FD-Score:-4.30|P-value:2.13E-6 ID:SGTC_2581|Compound:avocatin a|FD-Score:-3.31|P-value:2.04E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:-2.94|P-value:8.41E-4 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:3.22|P-value:2.73E-4 ID:SGTC_2659|Compound:sanguinarine|FD-Score:-3.05|P-value:5.69E-4 ID:SGTC_2761|Compound:levamisole|FD-Score:4.16|P-value:4.11E-6 ID:SGTC_2796|Compound:7949634|FD-Score:-3.57|P-value:6.67E-5 ID:SGTC_3114|Compound:9122766|FD-Score:-3.01|P-value:6.45E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:3.96|P-value:1.10E-5 ID:SGTC_12|Compound:0849-0099|FD-Score:3.03|P-value:5.84E-4 ID:SGTC_66|Compound:0109-0187|FD-Score:-6.06|P-value:4.44E-11 ID:SGTC_506|Compound:n9-isopropylolomoucine|FD-Score:2.94|P-value:8.06E-4 ID:SGTC_562|Compound:3772-2733|FD-Score:2.98|P-value:6.92E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:3.85|P-value:1.83E-5 ID:SGTC_633|Compound:0437-0093|FD-Score:-3.78|P-value:2.62E-5 ID:SGTC_1013|Compound:4112-3890|FD-Score:3.30|P-value:2.03E-4 ID:SGTC_1066|Compound:ketoconazole|FD-Score:-3.69|P-value:4.01E-5 ID:SGTC_1085|Compound:doxorubicin|FD-Score:2.96|P-value:7.38E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.62|P-value:5.52E-5 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-3.08|P-value:4.98E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:-4.31|P-value:2.08E-6 ID:SGTC_1303|Compound:1160-0052|FD-Score:-3.64|P-value:5.07E-5 ID:SGTC_1367|Compound:1812-0510|FD-Score:2.89|P-value:9.78E-4 ID:SGTC_1741|Compound:st038134|FD-Score:4.66|P-value:2.98E-7 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.69|P-value:3.97E-5 ID:SGTC_2001|Compound:4023502|FD-Score:3.11|P-value:4.23E-4 ID:SGTC_2006|Compound:4011487|FD-Score:4.06|P-value:6.58E-6 ID:SGTC_2024|Compound:5144227|FD-Score:-4.36|P-value:1.60E-6 ID:SGTC_2052|Compound:5265182|FD-Score:-3.02|P-value:6.27E-4 ID:SGTC_2056|Compound:5235370|FD-Score:-3.81|P-value:2.29E-5 ID:SGTC_2396|Compound:5342950|FD-Score:-3.19|P-value:3.33E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-3.01|P-value:6.40E-4 ID:SGTC_2428|Compound:5615643|FD-Score:-3.22|P-value:2.95E-4 ID:SGTC_2432|Compound:5630864|FD-Score:-4.25|P-value:2.71E-6 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.11|P-value:4.19E-4 ID:SGTC_2570|Compound:licareol|FD-Score:-4.30|P-value:2.13E-6 ID:SGTC_2581|Compound:avocatin a|FD-Score:-3.31|P-value:2.04E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:-2.94|P-value:8.41E-4 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:3.22|P-value:2.73E-4 ID:SGTC_2659|Compound:sanguinarine|FD-Score:-3.05|P-value:5.69E-4 ID:SGTC_2761|Compound:levamisole|FD-Score:4.16|P-value:4.11E-6 ID:SGTC_2796|Compound:7949634|FD-Score:-3.57|P-value:6.67E-5 ID:SGTC_3114|Compound:9122766|FD-Score:-3.01|P-value:6.45E-4

Top fitness defect scores for YJL058C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1741 st038134 4.66 10.2 µM 2.98E-7 15 1.59E-6
2 SGTC_2761 levamisole 4.16 97.9 µM 4.11E-6 13 1.59E-5
3 SGTC_2006 4011487 4.06 200.0 µM 6.58E-6 endomembrane recycling 79 2.40E-5
4 SGTC_6 0844-0013 3.96 19.8 µM 1.10E-5 87 3.77E-5
5 SGTC_621 3584-0052 3.85 32.1 µM 1.83E-5 29 5.89E-5
6 SGTC_1013 4112-3890 3.30 241.0 µM 2.03E-4 TSC3-RPN4 61 4.87E-4
7 SGTC_2590 3,4',5',6',7'-pentamethoxyflavone 3.22 100.0 µM 2.73E-4 60S ribosome export 51 6.33E-4
8 SGTC_2567 aureobasidin a 3.11 60.0 nM 4.19E-4 143 9.22E-4
9 SGTC_2001 4023502 3.11 57.7 µM 4.23E-4 mitochondrial processes 58 9.31E-4
10 SGTC_12 0849-0099 3.03 9.3 µM 5.84E-4 50 0.00123
11 SGTC_562 3772-2733 2.98 112.0 µM 6.92E-4 ERG2 142 0.00143
12 SGTC_1085 doxorubicin 2.96 7.7 µM 7.38E-4 anthracycline transcription coupled DNA repair 63 0.00152
13 SGTC_506 n9-isopropylolomoucine 2.94 153.0 µM 8.06E-4 52 0.00164
14 SGTC_1367 1812-0510 2.89 76.9 µM 9.78E-4 78 0.00194
15 SGTC_689 0833-0039 2.86 98.0 µM 0.00108 ergosterol depletion effects on membrane 71 0.00212
16 SGTC_2668 2',4'-dihydroxychalcone 2.79 15.1 µM 0.00137 48 0.00262
17 SGTC_2009 4012297 2.79 12.1 µM 0.00138 cell wall signaling 131 0.00264
18 SGTC_1344 1488-0097 2.76 29.1 µM 0.00153 72 0.00288
19 SGTC_2804 7954240 2.76 19.5 µM 0.00153 107 0.00289
20 SGTC_2054 5233173 2.75 129.0 µM 0.00159 mitochondrial response to ROS 153 0.00298

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.254 2.17E-50 YLR414C PUN1 hom Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress
0.216 1.18E-36 YDL039C PRM7 hom Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements
0.203 2.01E-32 YGL004C RPN14 hom Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p
0.171 2.14E-23 YKL004W AUR1 het Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.166 2.90E-22 YJR106W ECM27 hom Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants are hypersensitive to Papulacandin B; null mutants have increased plasmid loss; displays a two-hybrid interaction with Pdr5p
0.164 1.30E-21 YER067C-A_d YER067C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.163 1.86E-21 YJR092W BUD4 hom Anillin-like protein involved in bud-site selection; required for the axial budding pattern; localizes with septins to the bud neck in mitosis and may constitute an axial landmark for the next round of budding; required for the formation and disassembly of the double septin ring structure, and generally for septin organization; in vivo substrate of Cdc28p/Clb2p
0.163 2.12E-21 YKL131W_d YKL131W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.162 3.32E-21 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.152 9.49E-19 YPR151C SUE1 hom Mitochondrial protein required for degradation of unstable forms of cytochrome c
0.150 2.48E-18 YMR041C ARA2 hom NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase
0.148 8.13E-18 YDL012C YDL012C hom Tail-anchored plasma membrane protein with a conserved CYSTM module; possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype; YDL012C has a paralog, YBR016W, that arose from the whole genome duplication
0.147 8.59E-18 YLR263W RED1 hom Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p
0.147 1.32E-17 YDL174C DLD1 hom D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane
0.146 1.91E-17 YLR288C MEC3 hom DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1