YJL071W / ARG2

Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL071W / ARG2

Click on Significant Values for Screen Details ID:SGTC_54|Compound:0159-0055|FD-Score:5.75|P-value:3.55E-10 ID:SGTC_108|Compound:0509-0257|FD-Score:-2.97|P-value:7.40E-4 ID:SGTC_112|Compound:phenothiazine|FD-Score:3.54|P-value:7.37E-5 ID:SGTC_113|Compound:0099-0221|FD-Score:3.25|P-value:2.48E-4 ID:SGTC_139|Compound:0099-0334|FD-Score:3.46|P-value:1.04E-4 ID:SGTC_199|Compound:4063-0024|FD-Score:3.01|P-value:6.20E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:3.53|P-value:7.63E-5 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:2.97|P-value:7.27E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:3.33|P-value:1.80E-4 ID:SGTC_581|Compound:0958-0271|FD-Score:2.89|P-value:9.85E-4 ID:SGTC_723|Compound:4182-0001|FD-Score:-3.99|P-value:9.72E-6 ID:SGTC_887|Compound:0885-0007|FD-Score:4.01|P-value:8.83E-6 ID:SGTC_891|Compound:0929-0073|FD-Score:3.70|P-value:3.72E-5 ID:SGTC_936|Compound:3005-4620|FD-Score:5.17|P-value:1.49E-8 ID:SGTC_1250|Compound:0485-0349|FD-Score:-4.14|P-value:4.77E-6 ID:SGTC_1252|Compound:0493-0875|FD-Score:-5.75|P-value:3.85E-10 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.95|P-value:1.17E-5 ID:SGTC_1314|Compound:1222-0010|FD-Score:3.16|P-value:3.47E-4 ID:SGTC_1472|Compound:k841-0093|FD-Score:-3.39|P-value:1.45E-4 ID:SGTC_1487|Compound:3970-0795|FD-Score:3.59|P-value:5.89E-5 ID:SGTC_1489|Compound:4065-0444|FD-Score:2.91|P-value:9.00E-4 ID:SGTC_1494|Compound:4358-1428|FD-Score:-3.99|P-value:9.74E-6 ID:SGTC_1540|Compound:guanosine|FD-Score:4.39|P-value:1.28E-6 ID:SGTC_1578|Compound:josamycin|FD-Score:4.07|P-value:6.59E-6 ID:SGTC_1624|Compound:st003710|FD-Score:4.00|P-value:9.24E-6 ID:SGTC_1630|Compound:st005209|FD-Score:3.69|P-value:3.78E-5 ID:SGTC_1767|Compound:st044992|FD-Score:-2.94|P-value:8.29E-4 ID:SGTC_1889|Compound:st059080|FD-Score:3.67|P-value:4.14E-5 ID:SGTC_1898|Compound:st060199|FD-Score:3.48|P-value:9.37E-5 ID:SGTC_2185|Compound:6113692|FD-Score:2.92|P-value:8.75E-4 ID:SGTC_2293|Compound:7624732|FD-Score:4.60|P-value:4.23E-7 ID:SGTC_2319|Compound:6033012|FD-Score:3.20|P-value:2.99E-4 ID:SGTC_2346|Compound:9031566|FD-Score:3.78|P-value:2.53E-5 ID:SGTC_2415|Compound:st077333|FD-Score:3.47|P-value:9.77E-5 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:3.08|P-value:4.85E-4 ID:SGTC_2439|Compound:5492030|FD-Score:2.91|P-value:9.01E-4 ID:SGTC_2503|Compound:beta-caryophyllene alcohol|FD-Score:3.50|P-value:8.81E-5 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.24|P-value:2.55E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:3.19|P-value:3.15E-4 ID:SGTC_2832|Compound:7992762|FD-Score:4.79|P-value:1.41E-7 ID:SGTC_2978|Compound:9011784|FD-Score:3.54|P-value:7.37E-5 ID:SGTC_2981|Compound:9013952|FD-Score:-4.45|P-value:9.75E-7 ID:SGTC_2996|Compound:9066192|FD-Score:3.37|P-value:1.50E-4 ID:SGTC_3042|Compound:9091578|FD-Score:2.99|P-value:6.71E-4 ID:SGTC_3083|Compound:9117465|FD-Score:2.91|P-value:9.19E-4 ID:SGTC_3211|Compound:9128781|FD-Score:3.77|P-value:2.71E-5 ID:SGTC_3246|Compound:9135460|FD-Score:3.10|P-value:4.51E-4 ID:SGTC_54|Compound:0159-0055|FD-Score:5.75|P-value:3.55E-10 ID:SGTC_108|Compound:0509-0257|FD-Score:-2.97|P-value:7.40E-4 ID:SGTC_112|Compound:phenothiazine|FD-Score:3.54|P-value:7.37E-5 ID:SGTC_113|Compound:0099-0221|FD-Score:3.25|P-value:2.48E-4 ID:SGTC_139|Compound:0099-0334|FD-Score:3.46|P-value:1.04E-4 ID:SGTC_199|Compound:4063-0024|FD-Score:3.01|P-value:6.20E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:3.53|P-value:7.63E-5 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:2.97|P-value:7.27E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:3.33|P-value:1.80E-4 ID:SGTC_581|Compound:0958-0271|FD-Score:2.89|P-value:9.85E-4 ID:SGTC_723|Compound:4182-0001|FD-Score:-3.99|P-value:9.72E-6 ID:SGTC_887|Compound:0885-0007|FD-Score:4.01|P-value:8.83E-6 ID:SGTC_891|Compound:0929-0073|FD-Score:3.70|P-value:3.72E-5 ID:SGTC_936|Compound:3005-4620|FD-Score:5.17|P-value:1.49E-8 ID:SGTC_1250|Compound:0485-0349|FD-Score:-4.14|P-value:4.77E-6 ID:SGTC_1252|Compound:0493-0875|FD-Score:-5.75|P-value:3.85E-10 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.95|P-value:1.17E-5 ID:SGTC_1314|Compound:1222-0010|FD-Score:3.16|P-value:3.47E-4 ID:SGTC_1472|Compound:k841-0093|FD-Score:-3.39|P-value:1.45E-4 ID:SGTC_1487|Compound:3970-0795|FD-Score:3.59|P-value:5.89E-5 ID:SGTC_1489|Compound:4065-0444|FD-Score:2.91|P-value:9.00E-4 ID:SGTC_1494|Compound:4358-1428|FD-Score:-3.99|P-value:9.74E-6 ID:SGTC_1540|Compound:guanosine|FD-Score:4.39|P-value:1.28E-6 ID:SGTC_1578|Compound:josamycin|FD-Score:4.07|P-value:6.59E-6 ID:SGTC_1624|Compound:st003710|FD-Score:4.00|P-value:9.24E-6 ID:SGTC_1630|Compound:st005209|FD-Score:3.69|P-value:3.78E-5 ID:SGTC_1767|Compound:st044992|FD-Score:-2.94|P-value:8.29E-4 ID:SGTC_1889|Compound:st059080|FD-Score:3.67|P-value:4.14E-5 ID:SGTC_1898|Compound:st060199|FD-Score:3.48|P-value:9.37E-5 ID:SGTC_2185|Compound:6113692|FD-Score:2.92|P-value:8.75E-4 ID:SGTC_2293|Compound:7624732|FD-Score:4.60|P-value:4.23E-7 ID:SGTC_2319|Compound:6033012|FD-Score:3.20|P-value:2.99E-4 ID:SGTC_2346|Compound:9031566|FD-Score:3.78|P-value:2.53E-5 ID:SGTC_2415|Compound:st077333|FD-Score:3.47|P-value:9.77E-5 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:3.08|P-value:4.85E-4 ID:SGTC_2439|Compound:5492030|FD-Score:2.91|P-value:9.01E-4 ID:SGTC_2503|Compound:beta-caryophyllene alcohol|FD-Score:3.50|P-value:8.81E-5 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.24|P-value:2.55E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:3.19|P-value:3.15E-4 ID:SGTC_2832|Compound:7992762|FD-Score:4.79|P-value:1.41E-7 ID:SGTC_2978|Compound:9011784|FD-Score:3.54|P-value:7.37E-5 ID:SGTC_2981|Compound:9013952|FD-Score:-4.45|P-value:9.75E-7 ID:SGTC_2996|Compound:9066192|FD-Score:3.37|P-value:1.50E-4 ID:SGTC_3042|Compound:9091578|FD-Score:2.99|P-value:6.71E-4 ID:SGTC_3083|Compound:9117465|FD-Score:2.91|P-value:9.19E-4 ID:SGTC_3211|Compound:9128781|FD-Score:3.77|P-value:2.71E-5 ID:SGTC_3246|Compound:9135460|FD-Score:3.10|P-value:4.51E-4

Top fitness defect scores for YJL071W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_54 0159-0055 5.75 9.0 µM 3.55E-10 ERG2 23 4.37E-9
2 SGTC_936 3005-4620 5.17 401.0 µM 1.49E-8 7 1.15E-7
3 SGTC_2832 7992762 4.79 52.0 µM 1.41E-7 17 8.24E-7
4 SGTC_2293 7624732 4.60 200.0 µM 4.23E-7 14 2.16E-6
5 SGTC_1540 guanosine 4.39 70.6 µM 1.28E-6 3 5.70E-6
6 SGTC_1578 josamycin 4.07 24.2 µM 6.59E-6 10 2.40E-5
7 SGTC_887 0885-0007 4.01 9.6 µM 8.83E-6 endomembrane recycling 35 3.10E-5
8 SGTC_1624 st003710 4.00 6.2 µM 9.24E-6 calcium & mitochondrial duress 37 3.22E-5
9 SGTC_1263 0774-1208 3.95 64.8 µM 1.17E-5 49 3.96E-5
10 SGTC_2346 9031566 3.78 143.8 µM 2.53E-5 Golgi 20 7.79E-5
11 SGTC_3211 9128781 3.77 49.5 µM 2.71E-5 70 8.27E-5
12 SGTC_891 0929-0073 3.70 133.0 µM 3.72E-5 30 1.09E-4
13 SGTC_1630 st005209 3.69 31.3 µM 3.78E-5 30 1.11E-4
14 SGTC_1889 st059080 3.67 5.2 µM 4.14E-5 25 1.20E-4
15 SGTC_1487 3970-0795 3.59 2.8 µM 5.89E-5 DNA intercalators 18 1.64E-4
16 SGTC_112 phenothiazine 3.54 287.2 µM 7.37E-5 unfolded protein response 21 1.99E-4
17 SGTC_2978 9011784 3.54 43.0 µM 7.37E-5 48 2.00E-4
18 SGTC_221 trifluoperazine 3.53 10.3 µM 7.63E-5 NEO1-PIK1 234 2.06E-4
19 SGTC_2503 beta-caryophyllene alcohol 3.50 24.7 µM 8.81E-5 16 2.33E-4
20 SGTC_1898 st060199 3.48 18.6 µM 9.37E-5 24 2.46E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.236 1.64E-43 YOL140W ARG8 hom Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine
0.158 3.77E-20 YER069W ARG5,6 hom Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine
0.142 1.49E-16 YOL058W ARG1 hom Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate
0.109 2.06E-10 YOR303W CPA1 hom Small subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader
0.108 2.90E-10 YOR147W MDM32 hom Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34
0.105 1.25E-9 YER118C SHO1 hom Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p
0.103 1.91E-9 YJL088W ARG3 hom Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine
0.103 2.49E-9 YHR018C ARG4 hom Argininosuccinate lyase, catalyzes the final step in the arginine biosynthesis pathway
0.100 5.53E-9 YPR026W ATH1 hom Acid trehalase required for utilization of extracellular trehalose
0.098 1.42E-8 YOR062C_p YOR062C_p hom Protein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
0.092 9.15E-8 YER176W ECM32 hom DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes
0.090 1.72E-7 YER068C-A_d YER068C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.085 8.43E-7 YKL039W PTM1 hom Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication
0.083 1.33E-6 YHR065C RRP3 het Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity
0.080 3.30E-6 YHL044W_p YHL044W_p hom Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern