YJL138C / TIF2

Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL138C / TIF2

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:4.79|P-value:1.47E-7 ID:SGTC_16|Compound:erodoxin|FD-Score:-3.31|P-value:1.82E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.10|P-value:4.13E-4 ID:SGTC_228|Compound:caffeine|FD-Score:3.17|P-value:3.52E-4 ID:SGTC_341|Compound:1486-1430|FD-Score:3.23|P-value:2.80E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:3.21|P-value:3.01E-4 ID:SGTC_428|Compound:3448-4153|FD-Score:-2.98|P-value:6.58E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.02|P-value:7.44E-6 ID:SGTC_491|Compound:tetrandrine|FD-Score:3.85|P-value:1.96E-5 ID:SGTC_553|Compound:k007-0256|FD-Score:3.74|P-value:3.22E-5 ID:SGTC_579|Compound:4491-1009|FD-Score:-3.90|P-value:1.32E-5 ID:SGTC_673|Compound:1082-0474|FD-Score:3.56|P-value:7.11E-5 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.01|P-value:8.85E-6 ID:SGTC_733|Compound:4069-0007|FD-Score:-3.28|P-value:2.09E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:2.97|P-value:7.48E-4 ID:SGTC_897|Compound:1000-0139|FD-Score:3.45|P-value:1.13E-4 ID:SGTC_1029|Compound:k015-0030|FD-Score:4.44|P-value:1.01E-6 ID:SGTC_1042|Compound:0350-0096|FD-Score:-3.43|P-value:1.10E-4 ID:SGTC_1160|Compound:4092-0570|FD-Score:3.02|P-value:6.38E-4 ID:SGTC_1238|Compound:0333-0287|FD-Score:3.61|P-value:5.62E-5 ID:SGTC_1250|Compound:0485-0349|FD-Score:3.33|P-value:1.90E-4 ID:SGTC_1348|Compound:1493-0373|FD-Score:3.57|P-value:6.87E-5 ID:SGTC_1455|Compound:4262-0120|FD-Score:3.16|P-value:3.74E-4 ID:SGTC_1479|Compound:0549-0158|FD-Score:4.26|P-value:2.58E-6 ID:SGTC_1511|Compound:0906-3109|FD-Score:4.59|P-value:4.57E-7 ID:SGTC_1529|Compound:apigenin|FD-Score:2.95|P-value:8.23E-4 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:-3.10|P-value:4.27E-4 ID:SGTC_1678|Compound:st019265|FD-Score:3.74|P-value:3.25E-5 ID:SGTC_1714|Compound:st033236|FD-Score:-3.63|P-value:4.75E-5 ID:SGTC_1716|Compound:st033144|FD-Score:3.07|P-value:5.22E-4 ID:SGTC_1724|Compound:st037281|FD-Score:3.03|P-value:6.04E-4 ID:SGTC_1807|Compound:st050092|FD-Score:2.97|P-value:7.47E-4 ID:SGTC_1919|Compound:st067113|FD-Score:3.92|P-value:1.43E-5 ID:SGTC_1925|Compound:st057770|FD-Score:3.26|P-value:2.45E-4 ID:SGTC_2034|Compound:5197015|FD-Score:3.12|P-value:4.27E-4 ID:SGTC_2103|Compound:5378138|FD-Score:3.12|P-value:4.32E-4 ID:SGTC_2285|Compound:7945142|FD-Score:-4.22|P-value:2.78E-6 ID:SGTC_2373|Compound:9071805|FD-Score:2.91|P-value:9.62E-4 ID:SGTC_2385|Compound:9074692|FD-Score:-3.85|P-value:1.73E-5 ID:SGTC_2387|Compound:9077560|FD-Score:-3.55|P-value:6.57E-5 ID:SGTC_2401|Compound:5128683|FD-Score:-3.22|P-value:2.66E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-3.72|P-value:3.09E-5 ID:SGTC_2472|Compound:5377652|FD-Score:3.97|P-value:1.12E-5 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:4.67|P-value:2.94E-7 ID:SGTC_2593|Compound:nobiletin|FD-Score:-3.76|P-value:2.64E-5 ID:SGTC_2605|Compound:digitonin|FD-Score:3.21|P-value:3.08E-4 ID:SGTC_2608|Compound:thymoquinone|FD-Score:4.27|P-value:2.54E-6 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:4.42|P-value:1.13E-6 ID:SGTC_2629|Compound:bisabolol|FD-Score:5.90|P-value:1.39E-10 ID:SGTC_2712|Compound:st079126|FD-Score:3.54|P-value:7.86E-5 ID:SGTC_2804|Compound:7954240|FD-Score:3.40|P-value:1.38E-4 ID:SGTC_2807|Compound:7945238|FD-Score:-3.01|P-value:5.91E-4 ID:SGTC_2813|Compound:7971799|FD-Score:-3.07|P-value:4.79E-4 ID:SGTC_2894|Compound:9048625|FD-Score:4.03|P-value:8.24E-6 ID:SGTC_2951|Compound:9072950|FD-Score:-3.92|P-value:1.25E-5 ID:SGTC_3030|Compound:9087619|FD-Score:-3.83|P-value:1.85E-5 ID:SGTC_3286|Compound:9117135|FD-Score:3.44|P-value:1.19E-4 ID:SGTC_3346|Compound:9158254|FD-Score:4.10|P-value:5.81E-6 ID:SGTC_6|Compound:0844-0013|FD-Score:4.79|P-value:1.47E-7 ID:SGTC_16|Compound:erodoxin|FD-Score:-3.31|P-value:1.82E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.10|P-value:4.13E-4 ID:SGTC_228|Compound:caffeine|FD-Score:3.17|P-value:3.52E-4 ID:SGTC_341|Compound:1486-1430|FD-Score:3.23|P-value:2.80E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:3.21|P-value:3.01E-4 ID:SGTC_428|Compound:3448-4153|FD-Score:-2.98|P-value:6.58E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.02|P-value:7.44E-6 ID:SGTC_491|Compound:tetrandrine|FD-Score:3.85|P-value:1.96E-5 ID:SGTC_553|Compound:k007-0256|FD-Score:3.74|P-value:3.22E-5 ID:SGTC_579|Compound:4491-1009|FD-Score:-3.90|P-value:1.32E-5 ID:SGTC_673|Compound:1082-0474|FD-Score:3.56|P-value:7.11E-5 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.01|P-value:8.85E-6 ID:SGTC_733|Compound:4069-0007|FD-Score:-3.28|P-value:2.09E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:2.97|P-value:7.48E-4 ID:SGTC_897|Compound:1000-0139|FD-Score:3.45|P-value:1.13E-4 ID:SGTC_1029|Compound:k015-0030|FD-Score:4.44|P-value:1.01E-6 ID:SGTC_1042|Compound:0350-0096|FD-Score:-3.43|P-value:1.10E-4 ID:SGTC_1160|Compound:4092-0570|FD-Score:3.02|P-value:6.38E-4 ID:SGTC_1238|Compound:0333-0287|FD-Score:3.61|P-value:5.62E-5 ID:SGTC_1250|Compound:0485-0349|FD-Score:3.33|P-value:1.90E-4 ID:SGTC_1348|Compound:1493-0373|FD-Score:3.57|P-value:6.87E-5 ID:SGTC_1455|Compound:4262-0120|FD-Score:3.16|P-value:3.74E-4 ID:SGTC_1479|Compound:0549-0158|FD-Score:4.26|P-value:2.58E-6 ID:SGTC_1511|Compound:0906-3109|FD-Score:4.59|P-value:4.57E-7 ID:SGTC_1529|Compound:apigenin|FD-Score:2.95|P-value:8.23E-4 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:-3.10|P-value:4.27E-4 ID:SGTC_1678|Compound:st019265|FD-Score:3.74|P-value:3.25E-5 ID:SGTC_1714|Compound:st033236|FD-Score:-3.63|P-value:4.75E-5 ID:SGTC_1716|Compound:st033144|FD-Score:3.07|P-value:5.22E-4 ID:SGTC_1724|Compound:st037281|FD-Score:3.03|P-value:6.04E-4 ID:SGTC_1807|Compound:st050092|FD-Score:2.97|P-value:7.47E-4 ID:SGTC_1919|Compound:st067113|FD-Score:3.92|P-value:1.43E-5 ID:SGTC_1925|Compound:st057770|FD-Score:3.26|P-value:2.45E-4 ID:SGTC_2034|Compound:5197015|FD-Score:3.12|P-value:4.27E-4 ID:SGTC_2103|Compound:5378138|FD-Score:3.12|P-value:4.32E-4 ID:SGTC_2285|Compound:7945142|FD-Score:-4.22|P-value:2.78E-6 ID:SGTC_2373|Compound:9071805|FD-Score:2.91|P-value:9.62E-4 ID:SGTC_2385|Compound:9074692|FD-Score:-3.85|P-value:1.73E-5 ID:SGTC_2387|Compound:9077560|FD-Score:-3.55|P-value:6.57E-5 ID:SGTC_2401|Compound:5128683|FD-Score:-3.22|P-value:2.66E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-3.72|P-value:3.09E-5 ID:SGTC_2472|Compound:5377652|FD-Score:3.97|P-value:1.12E-5 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:4.67|P-value:2.94E-7 ID:SGTC_2593|Compound:nobiletin|FD-Score:-3.76|P-value:2.64E-5 ID:SGTC_2605|Compound:digitonin|FD-Score:3.21|P-value:3.08E-4 ID:SGTC_2608|Compound:thymoquinone|FD-Score:4.27|P-value:2.54E-6 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:4.42|P-value:1.13E-6 ID:SGTC_2629|Compound:bisabolol|FD-Score:5.90|P-value:1.39E-10 ID:SGTC_2712|Compound:st079126|FD-Score:3.54|P-value:7.86E-5 ID:SGTC_2804|Compound:7954240|FD-Score:3.40|P-value:1.38E-4 ID:SGTC_2807|Compound:7945238|FD-Score:-3.01|P-value:5.91E-4 ID:SGTC_2813|Compound:7971799|FD-Score:-3.07|P-value:4.79E-4 ID:SGTC_2894|Compound:9048625|FD-Score:4.03|P-value:8.24E-6 ID:SGTC_2951|Compound:9072950|FD-Score:-3.92|P-value:1.25E-5 ID:SGTC_3030|Compound:9087619|FD-Score:-3.83|P-value:1.85E-5 ID:SGTC_3286|Compound:9117135|FD-Score:3.44|P-value:1.19E-4 ID:SGTC_3346|Compound:9158254|FD-Score:4.10|P-value:5.81E-6

Top fitness defect scores for YJL138C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2629 bisabolol 5.90 100.0 µM 1.39E-10 5 1.85E-9
2 SGTC_6 0844-0013 4.79 19.8 µM 1.47E-7 46 8.16E-7
3 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 4.67 3.7 µM 2.94E-7 41 1.50E-6
4 SGTC_1511 0906-3109 4.59 28.5 µM 4.57E-7 excess fatty acid 27 2.21E-6
5 SGTC_1029 k015-0030 4.44 64.7 µM 1.01E-6 18 4.41E-6
6 SGTC_2615 purpurogallin-4-carboxylic acid 4.42 100.0 µM 1.13E-6 redox potentiating 53 4.87E-6
7 SGTC_2608 thymoquinone 4.27 42.7 µM 2.54E-6 12 9.93E-6
8 SGTC_1479 0549-0158 4.26 134.0 µM 2.58E-6 16 1.01E-5
9 SGTC_3346 9158254 4.10 43.6 µM 5.81E-6 10 2.05E-5
10 SGTC_2894 9048625 4.03 32.5 µM 8.24E-6 9 2.79E-5
11 SGTC_682 0kpi-0099 4.01 17.0 µM 8.85E-6 69 2.97E-5
12 SGTC_2472 5377652 3.97 49.4 µM 1.12E-5 TRP & mitochondrial translation 56 3.64E-5
13 SGTC_1919 st067113 3.92 52.3 µM 1.43E-5 TSC3-RPN4 29 4.52E-5
14 SGTC_491 tetrandrine 3.85 80.3 µM 1.96E-5 13 5.98E-5
15 SGTC_553 k007-0256 3.74 45.7 µM 3.22E-5 heme biosynthesis & mitochondrial translocase 25 9.25E-5
16 SGTC_1678 st019265 3.74 80.2 µM 3.25E-5 60S ribosome export 26 9.31E-5
17 SGTC_1238 0333-0287 3.61 48.8 µM 5.62E-5 Golgi 37 1.51E-4
18 SGTC_1348 1493-0373 3.57 93.8 µM 6.87E-5 22 1.80E-4
19 SGTC_673 1082-0474 3.56 110.0 µM 7.11E-5 RSC complex & mRNA processing 82 1.85E-4
20 SGTC_2712 st079126 3.54 47.7 µM 7.86E-5 29 2.02E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.140 3.56E-16 YML126C ERG13 het 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
0.136 3.16E-15 YER067C-A_d YER067C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.130 5.15E-14 YHL002W HSE1 hom Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes
0.122 1.25E-12 YMR205C PFK2 hom Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes
0.121 2.13E-12 YBR274W CHK1 hom Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase
0.120 2.60E-12 YDR051C DET1 hom Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel
0.118 6.02E-12 YMR191W SPG5 hom Protein required for proteasome assembly during quiescence; binds to base of the proteasome regulartory particle; required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources
0.117 1.21E-11 YNL242W ATG2 hom Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress
0.115 2.00E-11 YKR069W MET1 hom S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis
0.115 2.19E-11 YGR197C SNG1 hom Protein involved in resistance to nitrosoguanidine (MNNG) and 6-azauracil (6-AU); expression is regulated by transcription factors involved in multidrug resistance
0.115 2.80E-11 YLR288C MEC3 hom DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1
0.108 3.44E-10 YJL050W MTR4 het ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing
0.106 7.48E-10 YOR155C ISN1 hom Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms
0.103 1.99E-9 YHR021W-A_p ECM12_p hom Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity
0.103 2.22E-9 YDL049C KNH1 hom Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance