HIPHOP chemogenomics database

YJL138C / TIF2

Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication

Zygosity: Homozygous strain

fixedexpanded
Profile for YJL138C / TIF2

Click on Significant Values for Screen Details

Top fitness defect scores for YJL138C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2629	bisabolol	5.90	100.0 µM	1.39E-10		5	1.85E-9
2	SGTC_6	0844-0013	4.79	19.8 µM	1.47E-7		46	8.16E-7
3	SGTC_2494	18-aminoabieta-8,11,13-triene sulfate	4.67	3.7 µM	2.94E-7		41	1.50E-6
4	SGTC_1511	0906-3109	4.59	28.5 µM	4.57E-7	excess fatty acid	27	2.21E-6
5	SGTC_1029	k015-0030	4.44	64.7 µM	1.01E-6		18	4.41E-6
6	SGTC_2615	purpurogallin-4-carboxylic acid	4.42	100.0 µM	1.13E-6	redox potentiating	53	4.87E-6
7	SGTC_2608	thymoquinone	4.27	42.7 µM	2.54E-6		12	9.93E-6
8	SGTC_1479	0549-0158	4.26	134.0 µM	2.58E-6		16	1.01E-5
9	SGTC_3346	9158254	4.10	43.6 µM	5.81E-6		10	2.05E-5
10	SGTC_2894	9048625	4.03	32.5 µM	8.24E-6		9	2.79E-5
11	SGTC_682	0kpi-0099	4.01	17.0 µM	8.85E-6		69	2.97E-5
12	SGTC_2472	5377652	3.97	49.4 µM	1.12E-5	TRP & mitochondrial translation	56	3.64E-5
13	SGTC_1919	st067113	3.92	52.3 µM	1.43E-5	TSC3-RPN4	29	4.52E-5
14	SGTC_491	tetrandrine	3.85	80.3 µM	1.96E-5		13	5.98E-5
15	SGTC_553	k007-0256	3.74	45.7 µM	3.22E-5	heme biosynthesis & mitochondrial translocase	25	9.25E-5
16	SGTC_1678	st019265	3.74	80.2 µM	3.25E-5	60S ribosome export	26	9.31E-5
17	SGTC_1238	0333-0287	3.61	48.8 µM	5.62E-5	Golgi	37	1.51E-4
18	SGTC_1348	1493-0373	3.57	93.8 µM	6.87E-5		22	1.80E-4
19	SGTC_673	1082-0474	3.56	110.0 µM	7.11E-5	RSC complex & mRNA processing	82	1.85E-4
20	SGTC_2712	st079126	3.54	47.7 µM	7.86E-5		29	2.02E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.140	3.56E-16	YML126C	ERG13	het	3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
0.136	3.16E-15	YER067C-A_d	YER067C-A_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.130	5.15E-14	YHL002W	HSE1	hom	Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes
0.122	1.25E-12	YMR205C	PFK2	hom	Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes
0.121	2.13E-12	YBR274W	CHK1	hom	Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase
0.120	2.60E-12	YDR051C	DET1	hom	Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel
0.118	6.02E-12	YMR191W	SPG5	hom	Protein required for proteasome assembly during quiescence; binds to base of the proteasome regulartory particle; required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources
0.117	1.21E-11	YNL242W	ATG2	hom	Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress
0.115	2.00E-11	YKR069W	MET1	hom	S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis
0.115	2.19E-11	YGR197C	SNG1	hom	Protein involved in resistance to nitrosoguanidine (MNNG) and 6-azauracil (6-AU); expression is regulated by transcription factors involved in multidrug resistance
0.115	2.80E-11	YLR288C	MEC3	hom	DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1
0.108	3.44E-10	YJL050W	MTR4	het	ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing
0.106	7.48E-10	YOR155C	ISN1	hom	Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms
0.103	1.99E-9	YHR021W-A_p	ECM12_p	hom	Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity
0.103	2.22E-9	YDL049C	KNH1	hom	Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance