HIPHOP chemogenomics database

YJL159W / HSP150

O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication

Zygosity: Homozygous strain

fixedexpanded
Profile for YJL159W / HSP150

Click on Significant Values for Screen Details

Top fitness defect scores for YJL159W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2619	obliquin	35.50	87.9 µM	0	cell wall	1	1.48E-276
2	SGTC_916	4466-0038	9.83	42.0 nM	1.25E-25	cell wall	7	4.27E-23
3	SGTC_514	4466-0038	7.92	2.6 nM	2.59E-17	cell wall	42	1.21E-15
4	SGTC_3350	9150499	7.73	3.6 µM	1.37E-16	cell wall	8	5.38E-15
5	SGTC_206	4546-0025	7.71	72.0 µM	1.59E-16	NEO1	24	6.16E-15
6	SGTC_138	0850-0259	7.49	40.9 µM	1.06E-15	cell wall	69	3.35E-14
7	SGTC_1282	0886-0010	6.45	32.4 µM	4.20E-12	endomembrane recycling	8	5.63E-11
8	SGTC_221	trifluoperazine	6.42	10.3 µM	5.11E-12	NEO1-PIK1	112	6.72E-11
9	SGTC_2584	androsterone	6.22	48.7 µM	2.23E-11	cell wall	53	2.52E-10
10	SGTC_3311	9131146	5.86	2.9 µM	2.73E-10	cell wall	61	2.38E-9
11	SGTC_710	3448-4187	5.84	58.0 µM	3.10E-10	fatty acid desaturase (OLE1)	20	2.66E-9
12	SGTC_1230	0170-0365	5.25	3.8 µM	1.31E-8	endomembrane recycling	33	7.64E-8
13	SGTC_3321	9137793	5.16	26.8 µM	2.22E-8	cell wall	41	1.22E-7
14	SGTC_2625	euphol	4.95	100.0 µM	7.79E-8		14	3.77E-7
15	SGTC_15	0266-0086	4.73	27.1 µM	2.64E-7	endomembrane recycling	36	1.13E-6
16	SGTC_453	enantio-paf c16	4.32	10.0 µM	2.28E-6		118	7.80E-6
17	SGTC_813	k007-0158	4.30	173.0 µM	2.51E-6	fatty acid desaturase (OLE1)	35	8.49E-6
18	SGTC_542	3381-0244	4.25	89.3 µM	3.20E-6		33	1.06E-5
19	SGTC_2609	2,3,4-trihydroxy-4'-ethoxybenzophenone	4.16	70.0 µM	5.10E-6	redox potentiating	68	1.60E-5
20	SGTC_2350	9023895	4.16	38.9 µM	5.12E-6	ergosterol biosynthesis	24	1.61E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.486	1.92E-198	YMR124W_p	YMR124W_p	hom	Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.477	4.89E-190	YGL033W	HOP2	hom	Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair
0.456	1.90E-171	YML117W	NAB6	hom	Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP
0.418	1.23E-141	YLL032C	YLL032C	hom	Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.385	5.47E-119	YLL021W	SPA2	hom	Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate
0.377	1.75E-113	YBR020W	GAL1	hom	Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication
0.371	3.02E-110	YMR121C	RPL15B	hom	Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
0.362	1.58E-104	YOL003C	PFA4	hom	Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.351	5.26E-98	YEL061C	CIN8	hom	Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation
0.325	1.97E-83	YPL066W	RGL1	hom	Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
0.319	2.33E-80	YJR054W	KCH1	hom	Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.315	2.09E-78	YBR023C	CHS3	hom	Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
0.315	3.90E-78	YLR212C	TUB4	het	Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress
0.315	5.16E-78	YJR142W_p	YJR142W_p	hom	Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.313	2.36E-77	YOR091W	TMA46	hom	Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p