YJL172W / CPS1

Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL172W / CPS1

Click on Significant Values for Screen Details ID:SGTC_72|Compound:0744-0168|FD-Score:-3.48|P-value:4.19E-4 ID:SGTC_160|Compound:3453-0671|FD-Score:3.55|P-value:4.16E-4 ID:SGTC_162|Compound:k030-0015|FD-Score:3.30|P-value:9.46E-4 ID:SGTC_199|Compound:4063-0024|FD-Score:-4.60|P-value:5.27E-6 ID:SGTC_209|Compound:4077-0173|FD-Score:3.83|P-value:1.53E-4 ID:SGTC_311|Compound:1315-0136|FD-Score:3.72|P-value:2.26E-4 ID:SGTC_316|Compound:k060-0003|FD-Score:3.39|P-value:7.11E-4 ID:SGTC_317|Compound:4495-0002|FD-Score:3.40|P-value:6.90E-4 ID:SGTC_348|Compound:k064-0012|FD-Score:3.48|P-value:5.18E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:4.51|P-value:1.02E-5 ID:SGTC_383|Compound:2324-0395|FD-Score:-3.92|P-value:8.55E-5 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:3.34|P-value:8.18E-4 ID:SGTC_801|Compound:0866-0028|FD-Score:-3.40|P-value:5.52E-4 ID:SGTC_807|Compound:1315-0151|FD-Score:-3.41|P-value:5.35E-4 ID:SGTC_962|Compound:1254-0300|FD-Score:3.55|P-value:4.16E-4 ID:SGTC_1121|Compound:fusaric acid|FD-Score:4.02|P-value:7.41E-5 ID:SGTC_1122|Compound:capsaicin|FD-Score:6.71|P-value:1.04E-10 ID:SGTC_1481|Compound:3346-2004|FD-Score:4.28|P-value:2.72E-5 ID:SGTC_1894|Compound:3',6'-dihydroxyflavone|FD-Score:-3.24|P-value:9.30E-4 ID:SGTC_2763|Compound:lisinopril|FD-Score:-3.55|P-value:3.34E-4 ID:SGTC_2791|Compound:5663188|FD-Score:-3.51|P-value:3.77E-4 ID:SGTC_2854|Compound:9024559|FD-Score:3.58|P-value:3.76E-4 ID:SGTC_3202|Compound:9113761|FD-Score:-3.74|P-value:1.71E-4 ID:SGTC_72|Compound:0744-0168|FD-Score:-3.48|P-value:4.19E-4 ID:SGTC_160|Compound:3453-0671|FD-Score:3.55|P-value:4.16E-4 ID:SGTC_162|Compound:k030-0015|FD-Score:3.30|P-value:9.46E-4 ID:SGTC_199|Compound:4063-0024|FD-Score:-4.60|P-value:5.27E-6 ID:SGTC_209|Compound:4077-0173|FD-Score:3.83|P-value:1.53E-4 ID:SGTC_311|Compound:1315-0136|FD-Score:3.72|P-value:2.26E-4 ID:SGTC_316|Compound:k060-0003|FD-Score:3.39|P-value:7.11E-4 ID:SGTC_317|Compound:4495-0002|FD-Score:3.40|P-value:6.90E-4 ID:SGTC_348|Compound:k064-0012|FD-Score:3.48|P-value:5.18E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:4.51|P-value:1.02E-5 ID:SGTC_383|Compound:2324-0395|FD-Score:-3.92|P-value:8.55E-5 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:3.34|P-value:8.18E-4 ID:SGTC_801|Compound:0866-0028|FD-Score:-3.40|P-value:5.52E-4 ID:SGTC_807|Compound:1315-0151|FD-Score:-3.41|P-value:5.35E-4 ID:SGTC_962|Compound:1254-0300|FD-Score:3.55|P-value:4.16E-4 ID:SGTC_1121|Compound:fusaric acid|FD-Score:4.02|P-value:7.41E-5 ID:SGTC_1122|Compound:capsaicin|FD-Score:6.71|P-value:1.04E-10 ID:SGTC_1481|Compound:3346-2004|FD-Score:4.28|P-value:2.72E-5 ID:SGTC_1894|Compound:3',6'-dihydroxyflavone|FD-Score:-3.24|P-value:9.30E-4 ID:SGTC_2763|Compound:lisinopril|FD-Score:-3.55|P-value:3.34E-4 ID:SGTC_2791|Compound:5663188|FD-Score:-3.51|P-value:3.77E-4 ID:SGTC_2854|Compound:9024559|FD-Score:3.58|P-value:3.76E-4 ID:SGTC_3202|Compound:9113761|FD-Score:-3.74|P-value:1.71E-4

Top fitness defect scores for YJL172W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1122 capsaicin 6.71 818.6 nM 1.04E-10 2 9.42E-12
2 SGTC_362 0310-0100 4.51 9.0 µM 1.02E-5 RPP1 & pyrimidine depletion 20 3.22E-6
3 SGTC_1481 3346-2004 4.28 36.0 µM 2.72E-5 6 9.52E-6
4 SGTC_1121 fusaric acid 4.02 630.5 nM 7.41E-5 31 2.89E-5
5 SGTC_209 4077-0173 3.83 175.6 µM 1.53E-4 11 6.46E-5
6 SGTC_311 1315-0136 3.72 58.0 µM 2.26E-4 11 9.94E-5
7 SGTC_2854 9024559 3.58 71.4 µM 3.76E-4 36 1.75E-4
8 SGTC_160 3453-0671 3.55 289.7 µM 4.16E-4 50 1.96E-4
9 SGTC_962 1254-0300 3.55 21.5 µM 4.16E-4 37 1.96E-4
10 SGTC_348 k064-0012 3.48 10.7 µM 5.18E-4 60S ribosome export 25 2.50E-4
11 SGTC_317 4495-0002 3.40 170.0 µM 6.90E-4 24 3.43E-4
12 SGTC_316 k060-0003 3.39 58.6 µM 7.11E-4 26 3.55E-4
13 SGTC_392 N-ethylmaleimide 3.34 8.3 µM 8.18E-4 RNA pol III & RNase P/MRP 31 4.14E-4
14 SGTC_162 k030-0015 3.30 37.8 µM 9.46E-4 iron homeostasis 37 4.87E-4
15 SGTC_2291 7917025 3.24 104.3 µM 0.00112 iron homeostasis 82 5.88E-4
16 SGTC_2850 9015429 3.19 58.4 µM 0.00133 41 7.07E-4
17 SGTC_479 n-linoleoylglycine 3.16 10.0 µM 0.00148 27 8.01E-4
18 SGTC_1633 st005664 3.11 67.9 µM 0.00172 79 9.42E-4
19 SGTC_170 4197-0004 3.08 226.0 µM 0.00187 44 0.00103
20 SGTC_1724 st037281 3.07 8.3 µM 0.00192 100 0.00107

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.167 2.03E-22 YKL221W MCH2 hom Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport
0.153 5.26E-19 YDR315C IPK1 hom Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable
0.116 1.72E-11 YMR058W FET3 hom Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress
0.100 7.15E-9 YDR181C SAS4 hom Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p
0.099 7.89E-9 YLR177W_p YLR177W_p hom Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication
0.099 9.14E-9 YGL245W GUS1 het Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress
0.097 1.61E-8 YML012W ERV25 hom Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport
0.095 3.18E-8 YOL019W_p YOL019W_p hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication
0.094 4.57E-8 YMR176W ECM5 hom Non-essential protein of unknown function; contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress
0.092 9.18E-8 YMR004W MVP1 hom Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress
0.092 9.23E-8 YOL013W-A_p YOL013W-A_p hom Putative protein of unknown function; identified by SAGE
0.085 7.39E-7 YKL021C MAK11 het Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats
0.083 1.39E-6 YNR004W_p SWM2_p hom Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds
0.083 1.39E-6 YMR127C SAS2 hom Histone acetyltransferase (HAT) catalytic subunit of the SAS complex (Sas2p-Sas4p-Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family
0.083 1.58E-6 YMR031C EIS1 hom Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress