YJL186W / MNN5

Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL186W / MNN5

Click on Significant Values for Screen Details ID:SGTC_44|Compound:0929-0055|FD-Score:-4.58|P-value:1.77E-7 ID:SGTC_50|Compound:0929-0078|FD-Score:6.15|P-value:4.24E-12 ID:SGTC_61|Compound:0978-8327|FD-Score:4.48|P-value:3.27E-7 ID:SGTC_104|Compound:0074-0074|FD-Score:2.98|P-value:4.67E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:-2.80|P-value:9.41E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:2.87|P-value:7.10E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:3.11|P-value:2.78E-4 ID:SGTC_544|Compound:k015-0020|FD-Score:3.13|P-value:2.51E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:2.83|P-value:8.44E-4 ID:SGTC_637|Compound:1181-0519|FD-Score:3.35|P-value:9.92E-5 ID:SGTC_724|Compound:3910-0327|FD-Score:5.32|P-value:1.76E-9 ID:SGTC_962|Compound:1254-0300|FD-Score:3.94|P-value:6.09E-6 ID:SGTC_974|Compound:1326-0144|FD-Score:3.11|P-value:2.76E-4 ID:SGTC_977|Compound:1348-1378|FD-Score:3.36|P-value:9.62E-5 ID:SGTC_1020|Compound:4335-0961|FD-Score:4.17|P-value:1.87E-6 ID:SGTC_1045|Compound:1348-1485|FD-Score:2.91|P-value:6.23E-4 ID:SGTC_1069|Compound:diphenylcyclopropenone|FD-Score:3.35|P-value:9.97E-5 ID:SGTC_1116|Compound:jasmonic acid|FD-Score:4.69|P-value:9.86E-8 ID:SGTC_1153|Compound:4055-0057|FD-Score:3.10|P-value:2.92E-4 ID:SGTC_1162|Compound:k048-0130|FD-Score:3.06|P-value:3.38E-4 ID:SGTC_1218|Compound:1348-1416|FD-Score:3.89|P-value:7.73E-6 ID:SGTC_1266|Compound:0828-0227|FD-Score:2.93|P-value:5.77E-4 ID:SGTC_1278|Compound:0874-0585|FD-Score:3.41|P-value:7.63E-5 ID:SGTC_1288|Compound:0973-0013|FD-Score:-4.22|P-value:1.41E-6 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.06|P-value:3.35E-4 ID:SGTC_1484|Compound:3473-2379|FD-Score:3.85|P-value:9.45E-6 ID:SGTC_1495|Compound:4469-0029|FD-Score:2.99|P-value:4.49E-4 ID:SGTC_1516|Compound:st013856|FD-Score:4.53|P-value:2.46E-7 ID:SGTC_1590|Compound:st077108|FD-Score:-3.03|P-value:3.80E-4 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:3.06|P-value:3.38E-4 ID:SGTC_1733|Compound:st037357|FD-Score:2.94|P-value:5.45E-4 ID:SGTC_1736|Compound:st036774|FD-Score:-3.10|P-value:2.86E-4 ID:SGTC_1759|Compound:st043059|FD-Score:2.94|P-value:5.42E-4 ID:SGTC_1846|Compound:st056244|FD-Score:3.97|P-value:5.18E-6 ID:SGTC_1857|Compound:st056007|FD-Score:3.09|P-value:3.04E-4 ID:SGTC_1914|Compound:st062367|FD-Score:4.79|P-value:5.14E-8 ID:SGTC_2060|Compound:5238662|FD-Score:-3.15|P-value:2.36E-4 ID:SGTC_2110|Compound:5483698|FD-Score:3.20|P-value:1.89E-4 ID:SGTC_2353|Compound:9059630|FD-Score:3.16|P-value:2.25E-4 ID:SGTC_2369|Compound:9071472|FD-Score:3.15|P-value:2.32E-4 ID:SGTC_2401|Compound:5128683|FD-Score:2.81|P-value:9.16E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:3.00|P-value:4.36E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:2.81|P-value:9.15E-4 ID:SGTC_2569|Compound:tetrandrine|FD-Score:3.73|P-value:1.70E-5 ID:SGTC_2642|Compound:cholecalciferol|FD-Score:2.97|P-value:4.86E-4 ID:SGTC_2749|Compound:bexarotene|FD-Score:3.03|P-value:3.79E-4 ID:SGTC_2826|Compound:7992541|FD-Score:3.25|P-value:1.56E-4 ID:SGTC_2856|Compound:9022925|FD-Score:2.79|P-value:9.77E-4 ID:SGTC_2873|Compound:9040596|FD-Score:3.37|P-value:9.10E-5 ID:SGTC_2878|Compound:9043973|FD-Score:3.12|P-value:2.61E-4 ID:SGTC_2884|Compound:9058502|FD-Score:-2.79|P-value:9.79E-4 ID:SGTC_2896|Compound:9052656|FD-Score:3.90|P-value:7.63E-6 ID:SGTC_3020|Compound:9089140|FD-Score:2.79|P-value:9.76E-4 ID:SGTC_3050|Compound:9093513|FD-Score:3.33|P-value:1.10E-4 ID:SGTC_3094|Compound:9117742|FD-Score:3.27|P-value:1.43E-4 ID:SGTC_3198|Compound:9112019|FD-Score:3.57|P-value:3.65E-5 ID:SGTC_3319|Compound:9138450|FD-Score:-3.45|P-value:6.47E-5 ID:SGTC_44|Compound:0929-0055|FD-Score:-4.58|P-value:1.77E-7 ID:SGTC_50|Compound:0929-0078|FD-Score:6.15|P-value:4.24E-12 ID:SGTC_61|Compound:0978-8327|FD-Score:4.48|P-value:3.27E-7 ID:SGTC_104|Compound:0074-0074|FD-Score:2.98|P-value:4.67E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:-2.80|P-value:9.41E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:2.87|P-value:7.10E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:3.11|P-value:2.78E-4 ID:SGTC_544|Compound:k015-0020|FD-Score:3.13|P-value:2.51E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:2.83|P-value:8.44E-4 ID:SGTC_637|Compound:1181-0519|FD-Score:3.35|P-value:9.92E-5 ID:SGTC_724|Compound:3910-0327|FD-Score:5.32|P-value:1.76E-9 ID:SGTC_962|Compound:1254-0300|FD-Score:3.94|P-value:6.09E-6 ID:SGTC_974|Compound:1326-0144|FD-Score:3.11|P-value:2.76E-4 ID:SGTC_977|Compound:1348-1378|FD-Score:3.36|P-value:9.62E-5 ID:SGTC_1020|Compound:4335-0961|FD-Score:4.17|P-value:1.87E-6 ID:SGTC_1045|Compound:1348-1485|FD-Score:2.91|P-value:6.23E-4 ID:SGTC_1069|Compound:diphenylcyclopropenone|FD-Score:3.35|P-value:9.97E-5 ID:SGTC_1116|Compound:jasmonic acid|FD-Score:4.69|P-value:9.86E-8 ID:SGTC_1153|Compound:4055-0057|FD-Score:3.10|P-value:2.92E-4 ID:SGTC_1162|Compound:k048-0130|FD-Score:3.06|P-value:3.38E-4 ID:SGTC_1218|Compound:1348-1416|FD-Score:3.89|P-value:7.73E-6 ID:SGTC_1266|Compound:0828-0227|FD-Score:2.93|P-value:5.77E-4 ID:SGTC_1278|Compound:0874-0585|FD-Score:3.41|P-value:7.63E-5 ID:SGTC_1288|Compound:0973-0013|FD-Score:-4.22|P-value:1.41E-6 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.06|P-value:3.35E-4 ID:SGTC_1484|Compound:3473-2379|FD-Score:3.85|P-value:9.45E-6 ID:SGTC_1495|Compound:4469-0029|FD-Score:2.99|P-value:4.49E-4 ID:SGTC_1516|Compound:st013856|FD-Score:4.53|P-value:2.46E-7 ID:SGTC_1590|Compound:st077108|FD-Score:-3.03|P-value:3.80E-4 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:3.06|P-value:3.38E-4 ID:SGTC_1733|Compound:st037357|FD-Score:2.94|P-value:5.45E-4 ID:SGTC_1736|Compound:st036774|FD-Score:-3.10|P-value:2.86E-4 ID:SGTC_1759|Compound:st043059|FD-Score:2.94|P-value:5.42E-4 ID:SGTC_1846|Compound:st056244|FD-Score:3.97|P-value:5.18E-6 ID:SGTC_1857|Compound:st056007|FD-Score:3.09|P-value:3.04E-4 ID:SGTC_1914|Compound:st062367|FD-Score:4.79|P-value:5.14E-8 ID:SGTC_2060|Compound:5238662|FD-Score:-3.15|P-value:2.36E-4 ID:SGTC_2110|Compound:5483698|FD-Score:3.20|P-value:1.89E-4 ID:SGTC_2353|Compound:9059630|FD-Score:3.16|P-value:2.25E-4 ID:SGTC_2369|Compound:9071472|FD-Score:3.15|P-value:2.32E-4 ID:SGTC_2401|Compound:5128683|FD-Score:2.81|P-value:9.16E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:3.00|P-value:4.36E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:2.81|P-value:9.15E-4 ID:SGTC_2569|Compound:tetrandrine|FD-Score:3.73|P-value:1.70E-5 ID:SGTC_2642|Compound:cholecalciferol|FD-Score:2.97|P-value:4.86E-4 ID:SGTC_2749|Compound:bexarotene|FD-Score:3.03|P-value:3.79E-4 ID:SGTC_2826|Compound:7992541|FD-Score:3.25|P-value:1.56E-4 ID:SGTC_2856|Compound:9022925|FD-Score:2.79|P-value:9.77E-4 ID:SGTC_2873|Compound:9040596|FD-Score:3.37|P-value:9.10E-5 ID:SGTC_2878|Compound:9043973|FD-Score:3.12|P-value:2.61E-4 ID:SGTC_2884|Compound:9058502|FD-Score:-2.79|P-value:9.79E-4 ID:SGTC_2896|Compound:9052656|FD-Score:3.90|P-value:7.63E-6 ID:SGTC_3020|Compound:9089140|FD-Score:2.79|P-value:9.76E-4 ID:SGTC_3050|Compound:9093513|FD-Score:3.33|P-value:1.10E-4 ID:SGTC_3094|Compound:9117742|FD-Score:3.27|P-value:1.43E-4 ID:SGTC_3198|Compound:9112019|FD-Score:3.57|P-value:3.65E-5 ID:SGTC_3319|Compound:9138450|FD-Score:-3.45|P-value:6.47E-5

Top fitness defect scores for YJL186W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_50 0929-0078 6.15 56.4 µM 4.24E-12 23 3.82E-10
2 SGTC_724 3910-0327 5.32 137.0 µM 1.76E-9 ERG2 5 5.22E-8
3 SGTC_1914 st062367 4.79 72.6 µM 5.14E-8 9 8.19E-7
4 SGTC_1116 jasmonic acid 4.69 1.6 µM 9.86E-8 8 1.39E-6
5 SGTC_1516 st013856 4.53 110.0 µM 2.46E-7 6 2.94E-6
6 SGTC_61 0978-8327 4.48 98.6 µM 3.27E-7 ergosterol biosynthesis 18 3.71E-6
7 SGTC_1020 4335-0961 4.17 93.3 µM 1.87E-6 13 1.55E-5
8 SGTC_1846 st056244 3.97 14.8 µM 5.18E-6 25 3.56E-5
9 SGTC_962 1254-0300 3.94 21.5 µM 6.09E-6 23 4.07E-5
10 SGTC_2896 9052656 3.90 71.4 µM 7.63E-6 endomembrane recycling 119 4.89E-5
11 SGTC_1218 1348-1416 3.89 30.7 µM 7.73E-6 SWF1 & branched chain AA biosynthesis 15 4.95E-5
12 SGTC_1484 3473-2379 3.85 286.0 µM 9.45E-6 17 5.83E-5
13 SGTC_2569 tetrandrine 3.73 100.0 µM 1.70E-5 26 9.44E-5
14 SGTC_3198 9112019 3.57 49.5 µM 3.65E-5 mitochondrial processes 25 1.77E-4
15 SGTC_1278 0874-0585 3.41 172.0 µM 7.63E-5 29 3.23E-4
16 SGTC_2873 9040596 3.37 71.4 µM 9.10E-5 73 3.74E-4
17 SGTC_977 1348-1378 3.36 135.0 µM 9.62E-5 39 3.91E-4
18 SGTC_637 1181-0519 3.35 7.3 µM 9.92E-5 fatty acid desaturase (OLE1) 136 4.01E-4
19 SGTC_1069 diphenylcyclopropenone 3.35 8.5 µM 9.97E-5 51 4.03E-4
20 SGTC_3050 9093513 3.33 49.5 µM 1.10E-4 32 4.37E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.113 5.87E-11 YOL079W_d YOL079W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.097 1.61E-8 YGL090W LIF1 hom Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein
0.094 4.37E-8 YGR012W YGR012W hom Putative cysteine synthase, localized to the mitochondrial outer membrane
0.094 5.62E-8 YJR018W_d YJR018W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.084 1.16E-6 YKL215C OXP1 hom 5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
0.083 1.44E-6 YHL045W_d YHL045W_d hom Putative protein of unknown function; not an essential gene
0.083 1.44E-6 YPL199C_p YPL199C_p hom Putative protein of unknown function, predicted to be palmitoylated
0.083 1.67E-6 YPL113C_p YPL113C_p hom Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene
0.081 2.35E-6 YLR361C DCR2 hom Phosphoesterase involved in downregulation of the unfolded protein response, at least in part via dephosphorylation of Ire1p; dosage-dependent positive regulator of the G1/S phase transition through control of the timing of START
0.081 2.46E-6 YGR128C UTP8 het Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
0.077 7.48E-6 YNR069C BSC5 hom Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro
0.077 8.84E-6 YOR067C ALG8 hom Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p
0.076 9.47E-6 YGR068C ART5 hom Protein proposed to regulate the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target in the plasma membrane
0.076 1.17E-5 YER009W NTF2 het Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport
0.071 3.76E-5 YER155C BEM2 hom Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence