YJL187C / SWE1

Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL187C / SWE1

Click on Significant Values for Screen Details ID:SGTC_38|Compound:0929-0051|FD-Score:3.16|P-value:4.33E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:7.14|P-value:3.32E-14 ID:SGTC_65|Compound:0798-0905|FD-Score:7.39|P-value:4.15E-15 ID:SGTC_160|Compound:3453-0671|FD-Score:-5.49|P-value:5.37E-9 ID:SGTC_245|Compound:methotrexate|FD-Score:5.60|P-value:1.99E-9 ID:SGTC_254|Compound:nsc-64875|FD-Score:-3.58|P-value:9.89E-5 ID:SGTC_255|Compound:tunicamycin|FD-Score:6.15|P-value:5.04E-11 ID:SGTC_307|Compound:1501-1223|FD-Score:4.53|P-value:9.48E-7 ID:SGTC_309|Compound:k408-0051|FD-Score:3.52|P-value:1.04E-4 ID:SGTC_378|Compound:3970-0959|FD-Score:3.07|P-value:6.08E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:3.54|P-value:9.78E-5 ID:SGTC_422|Compound:cantharidin|FD-Score:2.95|P-value:9.40E-4 ID:SGTC_442|Compound:cape|FD-Score:4.05|P-value:1.01E-5 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:4.09|P-value:8.27E-6 ID:SGTC_501|Compound:nsc-95397|FD-Score:3.38|P-value:1.83E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:3.48|P-value:1.23E-4 ID:SGTC_650|Compound:1328-0195|FD-Score:4.29|P-value:3.20E-6 ID:SGTC_738|Compound:1315-0003|FD-Score:3.02|P-value:7.14E-4 ID:SGTC_794|Compound:k038-0031|FD-Score:3.19|P-value:3.91E-4 ID:SGTC_1029|Compound:k015-0030|FD-Score:2.94|P-value:9.55E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:5.38|P-value:7.68E-9 ID:SGTC_2035|Compound:5154463|FD-Score:3.94|P-value:1.68E-5 ID:SGTC_2054|Compound:5233173|FD-Score:3.12|P-value:5.06E-4 ID:SGTC_2056|Compound:5235370|FD-Score:3.58|P-value:8.01E-5 ID:SGTC_2329|Compound:6138490|FD-Score:4.83|P-value:1.93E-7 ID:SGTC_2338|Compound:7999974|FD-Score:-3.37|P-value:2.28E-4 ID:SGTC_2349|Compound:9023730|FD-Score:-3.20|P-value:4.50E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.16|P-value:2.94E-8 ID:SGTC_2589|Compound:lobaric acid|FD-Score:-4.18|P-value:6.75E-6 ID:SGTC_2591|Compound:skatole|FD-Score:-4.65|P-value:6.52E-7 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:5.68|P-value:1.23E-9 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.56|P-value:8.66E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.09|P-value:5.65E-4 ID:SGTC_2712|Compound:st079126|FD-Score:3.70|P-value:4.94E-5 ID:SGTC_2836|Compound:9010338|FD-Score:6.59|P-value:2.27E-12 ID:SGTC_2840|Compound:9008771|FD-Score:4.59|P-value:6.74E-7 ID:SGTC_2847|Compound:9005661|FD-Score:6.50|P-value:4.35E-12 ID:SGTC_2975|Compound:9084847|FD-Score:-3.10|P-value:6.49E-4 ID:SGTC_3082|Compound:9117008|FD-Score:3.26|P-value:2.91E-4 ID:SGTC_3140|Compound:9094243|FD-Score:-3.13|P-value:5.83E-4 ID:SGTC_3204|Compound:9114183|FD-Score:3.15|P-value:4.47E-4 ID:SGTC_3274|Compound:9138298|FD-Score:-4.05|P-value:1.23E-5 ID:SGTC_3286|Compound:9117135|FD-Score:-3.91|P-value:2.39E-5 ID:SGTC_3289|Compound:9119912|FD-Score:3.59|P-value:7.64E-5 ID:SGTC_3351|Compound:9150312|FD-Score:-3.14|P-value:5.49E-4 ID:SGTC_38|Compound:0929-0051|FD-Score:3.16|P-value:4.33E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:7.14|P-value:3.32E-14 ID:SGTC_65|Compound:0798-0905|FD-Score:7.39|P-value:4.15E-15 ID:SGTC_160|Compound:3453-0671|FD-Score:-5.49|P-value:5.37E-9 ID:SGTC_245|Compound:methotrexate|FD-Score:5.60|P-value:1.99E-9 ID:SGTC_254|Compound:nsc-64875|FD-Score:-3.58|P-value:9.89E-5 ID:SGTC_255|Compound:tunicamycin|FD-Score:6.15|P-value:5.04E-11 ID:SGTC_307|Compound:1501-1223|FD-Score:4.53|P-value:9.48E-7 ID:SGTC_309|Compound:k408-0051|FD-Score:3.52|P-value:1.04E-4 ID:SGTC_378|Compound:3970-0959|FD-Score:3.07|P-value:6.08E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:3.54|P-value:9.78E-5 ID:SGTC_422|Compound:cantharidin|FD-Score:2.95|P-value:9.40E-4 ID:SGTC_442|Compound:cape|FD-Score:4.05|P-value:1.01E-5 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:4.09|P-value:8.27E-6 ID:SGTC_501|Compound:nsc-95397|FD-Score:3.38|P-value:1.83E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:3.48|P-value:1.23E-4 ID:SGTC_650|Compound:1328-0195|FD-Score:4.29|P-value:3.20E-6 ID:SGTC_738|Compound:1315-0003|FD-Score:3.02|P-value:7.14E-4 ID:SGTC_794|Compound:k038-0031|FD-Score:3.19|P-value:3.91E-4 ID:SGTC_1029|Compound:k015-0030|FD-Score:2.94|P-value:9.55E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:5.38|P-value:7.68E-9 ID:SGTC_2035|Compound:5154463|FD-Score:3.94|P-value:1.68E-5 ID:SGTC_2054|Compound:5233173|FD-Score:3.12|P-value:5.06E-4 ID:SGTC_2056|Compound:5235370|FD-Score:3.58|P-value:8.01E-5 ID:SGTC_2329|Compound:6138490|FD-Score:4.83|P-value:1.93E-7 ID:SGTC_2338|Compound:7999974|FD-Score:-3.37|P-value:2.28E-4 ID:SGTC_2349|Compound:9023730|FD-Score:-3.20|P-value:4.50E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.16|P-value:2.94E-8 ID:SGTC_2589|Compound:lobaric acid|FD-Score:-4.18|P-value:6.75E-6 ID:SGTC_2591|Compound:skatole|FD-Score:-4.65|P-value:6.52E-7 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:5.68|P-value:1.23E-9 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.56|P-value:8.66E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.09|P-value:5.65E-4 ID:SGTC_2712|Compound:st079126|FD-Score:3.70|P-value:4.94E-5 ID:SGTC_2836|Compound:9010338|FD-Score:6.59|P-value:2.27E-12 ID:SGTC_2840|Compound:9008771|FD-Score:4.59|P-value:6.74E-7 ID:SGTC_2847|Compound:9005661|FD-Score:6.50|P-value:4.35E-12 ID:SGTC_2975|Compound:9084847|FD-Score:-3.10|P-value:6.49E-4 ID:SGTC_3082|Compound:9117008|FD-Score:3.26|P-value:2.91E-4 ID:SGTC_3140|Compound:9094243|FD-Score:-3.13|P-value:5.83E-4 ID:SGTC_3204|Compound:9114183|FD-Score:3.15|P-value:4.47E-4 ID:SGTC_3274|Compound:9138298|FD-Score:-4.05|P-value:1.23E-5 ID:SGTC_3286|Compound:9117135|FD-Score:-3.91|P-value:2.39E-5 ID:SGTC_3289|Compound:9119912|FD-Score:3.59|P-value:7.64E-5 ID:SGTC_3351|Compound:9150312|FD-Score:-3.14|P-value:5.49E-4

Top fitness defect scores for YJL187C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_65 0798-0905 7.39 35.0 µM 4.15E-15 calcium & mitochondrial duress 16 7.11E-14
2 SGTC_58 0957-0393 7.14 9.0 µM 3.32E-14 cell wall 158 4.73E-13
3 SGTC_2836 9010338 6.59 19.5 µM 2.27E-12 RPP1 & pyrimidine depletion 2 2.23E-11
4 SGTC_2847 9005661 6.50 71.4 µM 4.35E-12 1 4.04E-11
5 SGTC_255 tunicamycin 6.15 200.5 nM 5.04E-11 unfolded protein response 31 3.76E-10
6 SGTC_2599 2',3-dihydroxy-4,4',6'-trimethoxychalcone 5.68 100.0 µM 1.23E-9 6 6.92E-9
7 SGTC_245 methotrexate 5.60 289.6 µM 1.99E-9 16 1.07E-8
8 SGTC_1844 rapamycin 5.38 4.0 nM 7.68E-9 103 3.68E-8
9 SGTC_2582 agaric acid 5.16 100.0 µM 2.94E-8 redox potentiating 214 1.25E-7
10 SGTC_2329 6138490 4.83 200.0 µM 1.93E-7 11 6.96E-7
11 SGTC_2840 9008771 4.59 71.4 µM 6.74E-7 5 2.17E-6
12 SGTC_307 1501-1223 4.53 22.3 µM 9.48E-7 6 2.97E-6
13 SGTC_650 1328-0195 4.29 38.0 µM 3.20E-6 13 9.00E-6
14 SGTC_473 2-arachidonoylglycerol 4.09 10.0 µM 8.27E-6 20 2.14E-5
15 SGTC_442 cape 4.05 100.0 µM 1.01E-5 12 2.56E-5
16 SGTC_2035 5154463 3.94 94.6 µM 1.68E-5 15 4.09E-5
17 SGTC_2712 st079126 3.70 47.7 µM 4.94E-5 17 1.09E-4
18 SGTC_3289 9119912 3.59 71.4 µM 7.64E-5 25 1.63E-4
19 SGTC_2056 5235370 3.58 200.0 µM 8.01E-5 56 1.70E-4
20 SGTC_2651 1327-0036 3.56 5.0 µM 8.66E-5 189 1.82E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.288 3.99E-65 YGR217W CCH1 hom Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together
0.250 5.87E-49 YML103C NUP188 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188
0.244 1.68E-46 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.226 6.05E-40 YNL291C MID1 hom N-glycosylated integral membrane protein of the ER membrane and plasma membrane, functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer
0.209 1.58E-34 YIL160C POT1 hom 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
0.205 3.80E-33 YNL215W IES2 hom Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress
0.199 2.64E-31 YGR196C FYV8 hom Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.187 8.21E-28 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.179 1.44E-25 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.174 2.30E-24 YBR260C RGD1 hom GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.174 3.17E-24 YKL213C DOA1 hom WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress
0.173 6.64E-24 YHR036W BRL1 het Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
0.172 1.16E-23 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.172 1.14E-23 YCL005W LDB16 hom Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria
0.167 1.86E-22 YMR111C YMR111C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress