YJL216C / IMA5

Alpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; member of the IMA isomaltase family; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL216C / IMA5

Click on Significant Values for Screen Details ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:-3.55|P-value:5.69E-4 ID:SGTC_260|Compound:nsc-65238|FD-Score:3.73|P-value:3.12E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:4.14|P-value:7.18E-5 ID:SGTC_792|Compound:4534-1957|FD-Score:3.44|P-value:7.87E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:-3.85|P-value:2.12E-4 ID:SGTC_1008|Compound:4106-0037|FD-Score:-3.47|P-value:7.39E-4 ID:SGTC_1012|Compound:4112-3522|FD-Score:3.51|P-value:6.33E-4 ID:SGTC_1018|Compound:4259-0013|FD-Score:3.99|P-value:1.28E-4 ID:SGTC_1020|Compound:4335-0961|FD-Score:-3.76|P-value:2.89E-4 ID:SGTC_1025|Compound:k007-0731|FD-Score:3.88|P-value:1.86E-4 ID:SGTC_1217|Compound:1183-1422|FD-Score:3.54|P-value:5.80E-4 ID:SGTC_1227|Compound:3909-8010|FD-Score:4.77|P-value:6.00E-6 ID:SGTC_1376|Compound:2910-0861|FD-Score:4.15|P-value:6.98E-5 ID:SGTC_1617|Compound:st002906|FD-Score:4.21|P-value:5.69E-5 ID:SGTC_1719|Compound:st032227|FD-Score:3.45|P-value:7.79E-4 ID:SGTC_1774|Compound:st048371|FD-Score:3.38|P-value:9.65E-4 ID:SGTC_1846|Compound:st056244|FD-Score:3.97|P-value:1.37E-4 ID:SGTC_1850|Compound:st056238|FD-Score:3.70|P-value:3.42E-4 ID:SGTC_1950|Compound:st077106|FD-Score:4.18|P-value:6.37E-5 ID:SGTC_1956|Compound:st077085|FD-Score:-3.58|P-value:5.23E-4 ID:SGTC_2071|Compound:5230951|FD-Score:3.92|P-value:1.59E-4 ID:SGTC_2298|Compound:7976326|FD-Score:-3.97|P-value:1.38E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:4.73|P-value:7.13E-6 ID:SGTC_2772|Compound:vinpocetine|FD-Score:-3.62|P-value:4.49E-4 ID:SGTC_2774|Compound:tamoxifen|FD-Score:3.49|P-value:6.93E-4 ID:SGTC_2819|Compound:7996655|FD-Score:-3.87|P-value:1.95E-4 ID:SGTC_2864|Compound:9034755|FD-Score:4.06|P-value:9.88E-5 ID:SGTC_2888|Compound:9058547|FD-Score:-3.46|P-value:7.54E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:-3.55|P-value:5.69E-4 ID:SGTC_260|Compound:nsc-65238|FD-Score:3.73|P-value:3.12E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:4.14|P-value:7.18E-5 ID:SGTC_792|Compound:4534-1957|FD-Score:3.44|P-value:7.87E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:-3.85|P-value:2.12E-4 ID:SGTC_1008|Compound:4106-0037|FD-Score:-3.47|P-value:7.39E-4 ID:SGTC_1012|Compound:4112-3522|FD-Score:3.51|P-value:6.33E-4 ID:SGTC_1018|Compound:4259-0013|FD-Score:3.99|P-value:1.28E-4 ID:SGTC_1020|Compound:4335-0961|FD-Score:-3.76|P-value:2.89E-4 ID:SGTC_1025|Compound:k007-0731|FD-Score:3.88|P-value:1.86E-4 ID:SGTC_1217|Compound:1183-1422|FD-Score:3.54|P-value:5.80E-4 ID:SGTC_1227|Compound:3909-8010|FD-Score:4.77|P-value:6.00E-6 ID:SGTC_1376|Compound:2910-0861|FD-Score:4.15|P-value:6.98E-5 ID:SGTC_1617|Compound:st002906|FD-Score:4.21|P-value:5.69E-5 ID:SGTC_1719|Compound:st032227|FD-Score:3.45|P-value:7.79E-4 ID:SGTC_1774|Compound:st048371|FD-Score:3.38|P-value:9.65E-4 ID:SGTC_1846|Compound:st056244|FD-Score:3.97|P-value:1.37E-4 ID:SGTC_1850|Compound:st056238|FD-Score:3.70|P-value:3.42E-4 ID:SGTC_1950|Compound:st077106|FD-Score:4.18|P-value:6.37E-5 ID:SGTC_1956|Compound:st077085|FD-Score:-3.58|P-value:5.23E-4 ID:SGTC_2071|Compound:5230951|FD-Score:3.92|P-value:1.59E-4 ID:SGTC_2298|Compound:7976326|FD-Score:-3.97|P-value:1.38E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:4.73|P-value:7.13E-6 ID:SGTC_2772|Compound:vinpocetine|FD-Score:-3.62|P-value:4.49E-4 ID:SGTC_2774|Compound:tamoxifen|FD-Score:3.49|P-value:6.93E-4 ID:SGTC_2819|Compound:7996655|FD-Score:-3.87|P-value:1.95E-4 ID:SGTC_2864|Compound:9034755|FD-Score:4.06|P-value:9.88E-5 ID:SGTC_2888|Compound:9058547|FD-Score:-3.46|P-value:7.54E-4

Top fitness defect scores for YJL216C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1227 3909-8010 4.77 46.6 µM 6.00E-6 5 9.13E-7
2 SGTC_2419 paclitaxel 4.73 166.5 µM 7.13E-6 20 1.12E-6
3 SGTC_1617 st002906 4.21 78.0 µM 5.69E-5 22 1.30E-5
4 SGTC_1950 st077106 4.18 7.2 µM 6.37E-5 RNA processing & uracil transport 24 1.48E-5
5 SGTC_1376 2910-0861 4.15 41.3 µM 6.98E-5 15 1.65E-5
6 SGTC_346 4513-0042 4.14 7.2 µM 7.18E-5 azole & statin 37 1.71E-5
7 SGTC_2864 9034755 4.06 47.6 µM 9.88E-5 6 2.49E-5
8 SGTC_1018 4259-0013 3.99 28.1 µM 1.28E-4 14 3.37E-5
9 SGTC_1846 st056244 3.97 14.8 µM 1.37E-4 26 3.66E-5
10 SGTC_2071 5230951 3.92 39.9 µM 1.59E-4 TSC3-RPN4 18 4.34E-5
11 SGTC_1025 k007-0731 3.88 70.0 µM 1.86E-4 14 5.25E-5
12 SGTC_260 nsc-65238 3.73 4.0 µM 3.12E-4 endomembrane recycling 21 9.61E-5
13 SGTC_1850 st056238 3.70 17.0 µM 3.42E-4 RPP1 & pyrimidine depletion 27 1.07E-4
14 SGTC_1217 1183-1422 3.54 255.0 µM 5.80E-4 21 1.99E-4
15 SGTC_1012 4112-3522 3.51 18.0 µM 6.33E-4 32 2.21E-4
16 SGTC_2774 tamoxifen 3.49 10.9 µM 6.93E-4 23 2.46E-4
17 SGTC_1719 st032227 3.45 49.2 µM 7.79E-4 39 2.82E-4
18 SGTC_792 4534-1957 3.44 155.0 µM 7.87E-4 58 2.86E-4
19 SGTC_1774 st048371 3.38 48.5 µM 9.65E-4 RNA processing & uracil transport 42 3.63E-4
20 SGTC_323 4013-0906 3.36 34.3 µM 0.00103 ERG2 20 3.91E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.122 1.45E-12 YOL014W_p YOL014W_p hom Putative protein of unknown function
0.114 3.44E-11 YDL071C_d YDL071C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W
0.114 3.55E-11 YER173W RAD24 hom Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein
0.108 3.57E-10 YGL179C TOS3 hom Protein kinase, related to and functionally redundant with Elm1p and Sak1p for the phosphorylation and activation of Snf1p; functionally orthologous to LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome
0.101 3.89E-9 YGR039W_d YGR039W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the Autonomously Replicating Sequence ARS722
0.101 4.19E-9 YOL061W PRS5 hom 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress
0.091 1.44E-7 YGL203C KEX1 hom Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins
0.088 3.42E-7 YAR030C_d YAR030C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAR029W and the verified gene PRM9
0.081 2.28E-6 YER169W RPH1 hom JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication
0.079 4.99E-6 YGR145W ENP2 het Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p
0.077 7.84E-6 YNR073C_p YNR073C_p hom Putative mannitol dehydrogenase; identical in amino acid sequence to Dsf1p
0.074 1.68E-5 YNL336W COS1 hom Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
0.074 1.91E-5 YJR146W_p YJR146W_p hom Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2
0.072 2.87E-5 YOL124C TRM11 hom Catalytic subunit of an adoMet-dependent tRNA methyltransferase complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain
0.071 3.42E-5 YPR030W CSR2 hom Nuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication