YJR036C / HUL4

Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex

Zygosity: Homozygous strain
fixedexpanded
Profile for YJR036C / HUL4

Click on Significant Values for Screen Details ID:SGTC_1|Compound:0258-0098|FD-Score:-3.17|P-value:8.06E-4 ID:SGTC_61|Compound:0978-8327|FD-Score:3.80|P-value:8.44E-5 ID:SGTC_111|Compound:0083-0039|FD-Score:-3.14|P-value:8.69E-4 ID:SGTC_113|Compound:0099-0221|FD-Score:-4.59|P-value:2.43E-6 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:4.31|P-value:9.85E-6 ID:SGTC_128|Compound:0906-2981|FD-Score:-3.24|P-value:6.25E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:3.97|P-value:4.20E-5 ID:SGTC_189|Compound:3970-0742|FD-Score:-3.46|P-value:2.87E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:6.61|P-value:2.92E-11 ID:SGTC_417|Compound:st075190|FD-Score:-5.13|P-value:1.62E-7 ID:SGTC_599|Compound:1935-0052|FD-Score:5.68|P-value:9.36E-9 ID:SGTC_696|Compound:1560-0013|FD-Score:6.71|P-value:1.51E-11 ID:SGTC_840|Compound:0245-0196|FD-Score:3.62|P-value:1.68E-4 ID:SGTC_860|Compound:0443-0016|FD-Score:-3.46|P-value:2.82E-4 ID:SGTC_938|Compound:3126-1755|FD-Score:5.76|P-value:5.95E-9 ID:SGTC_950|Compound:1165-0508|FD-Score:4.17|P-value:1.81E-5 ID:SGTC_1085|Compound:doxorubicin|FD-Score:3.20|P-value:7.64E-4 ID:SGTC_1114|Compound:scopoletin|FD-Score:3.41|P-value:3.70E-4 ID:SGTC_1164|Compound:k060-0068|FD-Score:3.56|P-value:2.13E-4 ID:SGTC_1479|Compound:0549-0158|FD-Score:3.78|P-value:9.06E-5 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:3.24|P-value:6.81E-4 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:4.72|P-value:1.45E-6 ID:SGTC_1622|Compound:st003707|FD-Score:3.33|P-value:4.92E-4 ID:SGTC_1715|Compound:st032160|FD-Score:-3.38|P-value:3.81E-4 ID:SGTC_1717|Compound:st033143|FD-Score:-7.45|P-value:6.28E-14 ID:SGTC_1742|Compound:st038235|FD-Score:3.93|P-value:4.97E-5 ID:SGTC_1841|Compound:st054148|FD-Score:3.24|P-value:6.69E-4 ID:SGTC_1872|Compound:amphotericin b|FD-Score:3.56|P-value:2.18E-4 ID:SGTC_2041|Compound:5107442|FD-Score:3.18|P-value:8.28E-4 ID:SGTC_2237|Compound:6667747|FD-Score:3.52|P-value:2.50E-4 ID:SGTC_2239|Compound:6685329|FD-Score:5.32|P-value:6.72E-8 ID:SGTC_2242|Compound:6630449|FD-Score:-4.96|P-value:3.95E-7 ID:SGTC_2307|Compound:7538926|FD-Score:-3.25|P-value:6.04E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:4.85|P-value:7.95E-7 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.27|P-value:6.12E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:-4.14|P-value:1.91E-5 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:4.36|P-value:7.82E-6 ID:SGTC_2585|Compound:alliin|FD-Score:-5.28|P-value:7.57E-8 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:6.48|P-value:6.82E-11 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:3.24|P-value:6.71E-4 ID:SGTC_2982|Compound:9019404|FD-Score:3.48|P-value:2.85E-4 ID:SGTC_3078|Compound:9116413|FD-Score:3.25|P-value:6.60E-4 ID:SGTC_3299|Compound:9121982|FD-Score:-3.66|P-value:1.32E-4 ID:SGTC_1|Compound:0258-0098|FD-Score:-3.17|P-value:8.06E-4 ID:SGTC_61|Compound:0978-8327|FD-Score:3.80|P-value:8.44E-5 ID:SGTC_111|Compound:0083-0039|FD-Score:-3.14|P-value:8.69E-4 ID:SGTC_113|Compound:0099-0221|FD-Score:-4.59|P-value:2.43E-6 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:4.31|P-value:9.85E-6 ID:SGTC_128|Compound:0906-2981|FD-Score:-3.24|P-value:6.25E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:3.97|P-value:4.20E-5 ID:SGTC_189|Compound:3970-0742|FD-Score:-3.46|P-value:2.87E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:6.61|P-value:2.92E-11 ID:SGTC_417|Compound:st075190|FD-Score:-5.13|P-value:1.62E-7 ID:SGTC_599|Compound:1935-0052|FD-Score:5.68|P-value:9.36E-9 ID:SGTC_696|Compound:1560-0013|FD-Score:6.71|P-value:1.51E-11 ID:SGTC_840|Compound:0245-0196|FD-Score:3.62|P-value:1.68E-4 ID:SGTC_860|Compound:0443-0016|FD-Score:-3.46|P-value:2.82E-4 ID:SGTC_938|Compound:3126-1755|FD-Score:5.76|P-value:5.95E-9 ID:SGTC_950|Compound:1165-0508|FD-Score:4.17|P-value:1.81E-5 ID:SGTC_1085|Compound:doxorubicin|FD-Score:3.20|P-value:7.64E-4 ID:SGTC_1114|Compound:scopoletin|FD-Score:3.41|P-value:3.70E-4 ID:SGTC_1164|Compound:k060-0068|FD-Score:3.56|P-value:2.13E-4 ID:SGTC_1479|Compound:0549-0158|FD-Score:3.78|P-value:9.06E-5 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:3.24|P-value:6.81E-4 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:4.72|P-value:1.45E-6 ID:SGTC_1622|Compound:st003707|FD-Score:3.33|P-value:4.92E-4 ID:SGTC_1715|Compound:st032160|FD-Score:-3.38|P-value:3.81E-4 ID:SGTC_1717|Compound:st033143|FD-Score:-7.45|P-value:6.28E-14 ID:SGTC_1742|Compound:st038235|FD-Score:3.93|P-value:4.97E-5 ID:SGTC_1841|Compound:st054148|FD-Score:3.24|P-value:6.69E-4 ID:SGTC_1872|Compound:amphotericin b|FD-Score:3.56|P-value:2.18E-4 ID:SGTC_2041|Compound:5107442|FD-Score:3.18|P-value:8.28E-4 ID:SGTC_2237|Compound:6667747|FD-Score:3.52|P-value:2.50E-4 ID:SGTC_2239|Compound:6685329|FD-Score:5.32|P-value:6.72E-8 ID:SGTC_2242|Compound:6630449|FD-Score:-4.96|P-value:3.95E-7 ID:SGTC_2307|Compound:7538926|FD-Score:-3.25|P-value:6.04E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:4.85|P-value:7.95E-7 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.27|P-value:6.12E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:-4.14|P-value:1.91E-5 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:4.36|P-value:7.82E-6 ID:SGTC_2585|Compound:alliin|FD-Score:-5.28|P-value:7.57E-8 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:6.48|P-value:6.82E-11 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:3.24|P-value:6.71E-4 ID:SGTC_2982|Compound:9019404|FD-Score:3.48|P-value:2.85E-4 ID:SGTC_3078|Compound:9116413|FD-Score:3.25|P-value:6.60E-4 ID:SGTC_3299|Compound:9121982|FD-Score:-3.66|P-value:1.32E-4

Top fitness defect scores for YJR036C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_696 1560-0013 6.71 611.0 µM 1.51E-11 6 9.93E-12
2 SGTC_219 mitoxanthrone 6.61 164.4 µM 2.92E-11 DNA intercalators 17 1.94E-11
3 SGTC_2588 indole-3-carbinol 6.48 100.0 µM 6.82E-11 8 4.60E-11
4 SGTC_938 3126-1755 5.76 342.0 µM 5.95E-9 1 4.32E-9
5 SGTC_599 1935-0052 5.68 86.5 µM 9.36E-9 1 6.84E-9
6 SGTC_2239 6685329 5.32 187.6 µM 6.72E-8 7 5.08E-8
7 SGTC_2566 crystal violet carbinol 4.85 226.0 nM 7.95E-7 22 6.26E-7
8 SGTC_1565 sinapyl alcohol 4.72 95.1 µM 1.45E-6 11 1.15E-6
9 SGTC_2579 2',5'-dihydroxy-4-methoxychalcone 4.36 13.2 µM 7.82E-6 18 6.40E-6
10 SGTC_120 rhodamine 6G 4.31 52.3 µM 9.85E-6 mitochondrial processes 22 8.09E-6
11 SGTC_950 1165-0508 4.17 94.1 µM 1.81E-5 23 1.50E-5
12 SGTC_151 3013-0144 3.97 2.3 µM 4.20E-5 iron homeostasis 27 3.54E-5
13 SGTC_1742 st038235 3.93 28.9 µM 4.97E-5 mitochondrial stress 116 4.20E-5
14 SGTC_61 0978-8327 3.80 98.6 µM 8.44E-5 ergosterol biosynthesis 34 7.19E-5
15 SGTC_1479 0549-0158 3.78 134.0 µM 9.06E-5 31 7.73E-5
16 SGTC_840 0245-0196 3.62 390.0 µM 1.68E-4 19 1.45E-4
17 SGTC_1164 k060-0068 3.56 93.7 µM 2.13E-4 43 1.84E-4
18 SGTC_1872 amphotericin b 3.56 148.0 nM 2.18E-4 RNA pol III & RNase P/MRP 39 1.88E-4
19 SGTC_2237 6667747 3.52 47.2 µM 2.50E-4 24 2.17E-4
20 SGTC_2982 9019404 3.48 26.0 µM 2.85E-4 33 2.48E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.095 3.14E-8 YDR406W PDR15 hom Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element
0.091 1.10E-7 YKL004W AUR1 het Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.091 1.43E-7 YER053C-A YER053C-A hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
0.088 3.17E-7 YAL017W PSK1 hom PAS domain-containing serine/threonine protein kinase; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status; PSK1 has a paralog, PSK2, that arose from the whole genome duplication
0.087 4.03E-7 YKL012W PRP40 het U1 snRNP protein involved in splicing, interacts with the branchpoint-binding protein during the formation of the second commitment complex
0.087 5.40E-7 YPL262W FUM1 hom Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria
0.083 1.39E-6 YDL166C FAP7 het Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D
0.082 1.99E-6 YBR259W YBR259W hom Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress
0.081 2.93E-6 YOL098C_p YOL098C_p hom Putative metalloprotease
0.081 2.99E-6 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.080 3.31E-6 YDR164C SEC1 het Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function
0.080 3.36E-6 YER166W DNF1 hom Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase
0.079 4.14E-6 YFR032C_p RRT5_p hom Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation
0.079 4.83E-6 YOR202W HIS3 hom Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p
0.077 7.34E-6 YCR047C BUD23 hom Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern