YJR054W / KCH1

Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate

Zygosity: Homozygous strain
fixedexpanded
Profile for YJR054W / KCH1

Click on Significant Values for Screen Details

Top fitness defect scores for YJR054W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_58 0957-0393 20.20 9.0 µM 3.33E-107 cell wall 9 2.19E-91
2 SGTC_916 4466-0038 12.60 42.0 nM 7.02E-43 cell wall 3 9.75E-37
3 SGTC_138 0850-0259 12.00 40.9 µM 4.47E-39 cell wall 28 1.67E-33
4 SGTC_304 1310-0298 6.81 97.0 µM 7.34E-14 cell wall 12 4.73E-12
5 SGTC_2584 androsterone 6.50 48.7 µM 8.90E-13 cell wall 46 3.96E-11
6 SGTC_158 k038-0087 6.45 26.4 µM 1.38E-12 ubiquinone biosynthesis & proteasome 60 5.76E-11
7 SGTC_690 glyoxal 5.57 20.3 µM 7.90E-10 51 1.30E-8
8 SGTC_1712 st032288 5.48 53.4 µM 1.39E-9 calcium & mitochondrial duress 92 2.10E-8
9 SGTC_682 0kpi-0099 5.47 17.0 µM 1.47E-9 30 2.20E-8
10 SGTC_266 formosanin C 5.39 406.0 nM 2.56E-9 sphingolipid biosynthesis & PDR1 38 3.53E-8
11 SGTC_923 1900-2434 4.98 73.3 µM 3.28E-8 RNA processing & uracil transport 14 3.12E-7
12 SGTC_514 4466-0038 4.30 2.6 nM 1.59E-6 cell wall 135 8.59E-6
13 SGTC_2970 9090938 4.25 41.9 µM 2.10E-6 RNA processing & uracil transport 30 1.09E-5
14 SGTC_558 k064-0027 4.23 21.3 µM 2.22E-6 DNA damage response 19 1.15E-5
15 SGTC_951 1171-0579 4.11 82.7 µM 4.16E-6 cell wall 71 1.96E-5
16 SGTC_1514 1189-1591 4.09 158.0 µM 4.74E-6 31 2.19E-5
17 SGTC_121 0557-0495 4.08 61.3 µM 4.94E-6 calcium & mitochondrial duress 53 2.27E-5
18 SGTC_2602 kawain 3.87 100.0 µM 1.36E-5 30 5.39E-5
19 SGTC_1695 st026914 3.84 34.5 µM 1.60E-5 21 6.22E-5
20 SGTC_206 4546-0025 3.67 72.0 µM 3.44E-5 NEO1 88 1.20E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.534 7.11E-247 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.505 1.93E-216 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.461 6.50E-176 YCL005W LDB16 hom Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria
0.449 5.34E-166 YML117W NAB6 hom Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP
0.444 1.22E-161 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.442 2.06E-160 YKL033W-A_p YKL033W-A_p hom Putative protein of unknown function; similar to uncharacterized proteins from other fungi
0.439 2.15E-158 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.439 2.16E-158 YKL076C_d PSY1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.429 3.00E-150 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.429 3.58E-150 YDL021W GPM2 hom Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event
0.424 2.77E-146 YPL269W KAR9 hom Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase
0.422 2.99E-145 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.407 7.39E-134 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.403 5.81E-131 YNL314W DAL82 hom Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
0.395 1.17E-125 YPL115C BEM3 hom Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly