Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress

Zygosity: Homozygous strain
Profile for YJR056C / YJR056C

Click on Significant Values for Screen Details

Top fitness defect scores for YJR056C deletion by condition

RankScreen IDConditionFD ScoreConc
1 SGTC_2573 2',4'-dihydroxychalcone 5.65 15.0 µM 5.26E-12 17 8.09E-9
2 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 4.73 3.7 µM 6.06E-9 38 1.13E-6
3 SGTC_2060 5238662 4.43 140.0 µM 4.54E-8 19 4.67E-6
4 SGTC_1344 1488-0097 4.14 29.1 µM 2.92E-7 13 1.72E-5
5 SGTC_1119 parthenolide 4.10 318.5 nM 3.66E-7 TSC3-RPN4 33 2.02E-5
6 SGTC_2751 anetholtrithion 4.06 17.2 µM 4.75E-7 25 2.43E-5
7 SGTC_2563 tomatine 3.92 8.5 µM 1.12E-6 47 4.45E-5
8 SGTC_1733 st037357 3.57 54.9 µM 8.15E-6 60S ribosome export 46 1.80E-4
9 SGTC_1203 3992-3522 3.48 103.0 µM 1.30E-5 58 2.50E-4
10 SGTC_753 1322-0200 3.45 289.0 µM 1.50E-5 39 2.76E-4
11 SGTC_2755 lomerizine 3.40 42.7 µM 2.00E-5 42 3.38E-4
12 SGTC_515 4092-1064 3.32 102.0 µM 3.05E-5 plasma membrane duress 80 4.57E-4
13 SGTC_2145 5634607 3.24 200.0 µM 4.38E-5 22 5.90E-4
14 SGTC_2438 5259590 3.24 200.0 µM 4.52E-5 33 6.03E-4
15 SGTC_739 1486-1183 3.20 203.0 µM 5.37E-5 38 6.81E-4
16 SGTC_861 0443-0273 3.19 110.0 µM 5.80E-5 34 7.19E-4
17 SGTC_549 1661-1316 3.17 519.0 µM 6.44E-5 46 7.75E-4
18 SGTC_3168 9103419 3.14 49.5 µM 7.29E-5 60S ribosome export 16 8.45E-4
19 SGTC_503 tyrphostin 8 3.04 294.0 µM 1.18E-4 TSC3-RPN4 71 0.00119
20 SGTC_2580 diallyl disulphide 3.04 81.4 µM 1.19E-4 58 0.00120

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.232 2.80E-42 YHR003C YHR003C hom Protein of unknown function, localized to the mitochondrial outer membrane
0.204 5.31E-33 YBR118W TEF2 hom Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication
0.201 4.92E-32 YDR046C BAP3 hom Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication
0.195 4.06E-30 YER064C VHR2 hom Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.193 1.89E-29 YER067C-A_d YER067C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.186 1.94E-27 YLR288C MEC3 hom DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1
0.184 4.94E-27 YLL046C RNP1 hom Ribonucleoprotein that contains two RNA recognition motifs (RRM)
0.179 1.44E-25 YKL179C COY1 hom Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
0.174 2.97E-24 YEL004W YEA4 hom Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER
0.172 1.35E-23 YDR534C FIT1 hom Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall
0.171 1.54E-23 YML053C_p YML053C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; overexpression causes a cell cycle delay or arrest; YML053C is not an essential gene
0.170 4.83E-23 YLR205C HMX1 hom ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants
0.169 6.51E-23 YEL049W PAU2 hom Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme
0.168 1.49E-22 YGR032W GSC2 hom Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p)
0.167 1.81E-22 YLR267W_p BOP2_p hom Protein of unknown function