YJR083C / ACF4

Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate

Zygosity: Homozygous strain
fixedexpanded
Profile for YJR083C / ACF4

Click on Significant Values for Screen Details ID:SGTC_62|Compound:0180-0423|FD-Score:-3.04|P-value:7.01E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:-3.23|P-value:3.40E-4 ID:SGTC_114|Compound:0364-0001|FD-Score:3.28|P-value:2.59E-4 ID:SGTC_164|Compound:4100-3994|FD-Score:2.98|P-value:7.87E-4 ID:SGTC_217|Compound:ketoconazole|FD-Score:-2.95|P-value:9.51E-4 ID:SGTC_256|Compound:clotrimazole|FD-Score:-3.98|P-value:1.38E-5 ID:SGTC_263|Compound:alizarin|FD-Score:2.94|P-value:9.18E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.26|P-value:3.07E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:3.48|P-value:1.16E-4 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:-3.37|P-value:1.93E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:4.13|P-value:6.13E-6 ID:SGTC_518|Compound:1021-0119|FD-Score:3.75|P-value:3.70E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:4.43|P-value:1.39E-6 ID:SGTC_555|Compound:4296-0685|FD-Score:-3.45|P-value:1.42E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:4.30|P-value:2.75E-6 ID:SGTC_575|Compound:k072-0246|FD-Score:2.93|P-value:9.72E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:3.23|P-value:3.14E-4 ID:SGTC_658|Compound:3253-0951|FD-Score:4.22|P-value:3.93E-6 ID:SGTC_689|Compound:0833-0039|FD-Score:-3.74|P-value:4.07E-5 ID:SGTC_823|Compound:0417-1665|FD-Score:-3.07|P-value:6.21E-4 ID:SGTC_940|Compound:3232-1287|FD-Score:3.02|P-value:6.90E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:3.93|P-value:1.62E-5 ID:SGTC_1215|Compound:3852-0577|FD-Score:3.06|P-value:5.96E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.08|P-value:5.45E-4 ID:SGTC_1251|Compound:0490-5699|FD-Score:3.55|P-value:8.75E-5 ID:SGTC_1271|Compound:0832-2808|FD-Score:2.98|P-value:8.02E-4 ID:SGTC_1366|Compound:1683-0113|FD-Score:-3.63|P-value:6.65E-5 ID:SGTC_1508|Compound:1080-0625|FD-Score:3.76|P-value:3.41E-5 ID:SGTC_1547|Compound:harmine|FD-Score:-5.18|P-value:2.47E-8 ID:SGTC_1549|Compound:4'-methoxyflavonol|FD-Score:-3.52|P-value:1.05E-4 ID:SGTC_1571|Compound:st070080|FD-Score:-3.70|P-value:4.92E-5 ID:SGTC_1658|Compound:st012944|FD-Score:5.52|P-value:2.73E-9 ID:SGTC_1781|Compound:st038725|FD-Score:-2.96|P-value:9.30E-4 ID:SGTC_1782|Compound:st039423|FD-Score:-3.01|P-value:7.75E-4 ID:SGTC_1786|Compound:berberine|FD-Score:-5.94|P-value:1.98E-10 ID:SGTC_1787|Compound:fluconazole|FD-Score:-4.70|P-value:3.75E-7 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-3.53|P-value:9.98E-5 ID:SGTC_1866|Compound:st057638|FD-Score:2.94|P-value:9.22E-4 ID:SGTC_2021|Compound:4019055|FD-Score:4.10|P-value:7.37E-6 ID:SGTC_2175|Compound:5807670|FD-Score:3.25|P-value:2.91E-4 ID:SGTC_2358|Compound:9037157|FD-Score:3.22|P-value:3.24E-4 ID:SGTC_2415|Compound:st077333|FD-Score:3.77|P-value:3.37E-5 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:3.13|P-value:4.64E-4 ID:SGTC_2482|Compound:5981709|FD-Score:-3.49|P-value:1.18E-4 ID:SGTC_2484|Compound:5809818|FD-Score:-3.33|P-value:2.26E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.98|P-value:1.25E-5 ID:SGTC_2829|Compound:7992743|FD-Score:4.61|P-value:5.34E-7 ID:SGTC_2830|Compound:7992007|FD-Score:-4.20|P-value:5.02E-6 ID:SGTC_2831|Compound:7999036|FD-Score:4.65|P-value:4.35E-7 ID:SGTC_2834|Compound:9001755|FD-Score:3.32|P-value:2.21E-4 ID:SGTC_2935|Compound:9038016|FD-Score:3.29|P-value:2.44E-4 ID:SGTC_2990|Compound:9074105|FD-Score:-3.27|P-value:2.92E-4 ID:SGTC_2992|Compound:9074591|FD-Score:-3.29|P-value:2.64E-4 ID:SGTC_2997|Compound:9067075|FD-Score:5.13|P-value:2.92E-8 ID:SGTC_3005|Compound:9076496|FD-Score:-3.35|P-value:2.10E-4 ID:SGTC_3284|Compound:9108171|FD-Score:3.75|P-value:3.70E-5 ID:SGTC_3304|Compound:9125678|FD-Score:4.39|P-value:1.71E-6 ID:SGTC_3354|Compound:9151612|FD-Score:3.06|P-value:5.89E-4 ID:SGTC_62|Compound:0180-0423|FD-Score:-3.04|P-value:7.01E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:-3.23|P-value:3.40E-4 ID:SGTC_114|Compound:0364-0001|FD-Score:3.28|P-value:2.59E-4 ID:SGTC_164|Compound:4100-3994|FD-Score:2.98|P-value:7.87E-4 ID:SGTC_217|Compound:ketoconazole|FD-Score:-2.95|P-value:9.51E-4 ID:SGTC_256|Compound:clotrimazole|FD-Score:-3.98|P-value:1.38E-5 ID:SGTC_263|Compound:alizarin|FD-Score:2.94|P-value:9.18E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.26|P-value:3.07E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:3.48|P-value:1.16E-4 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:-3.37|P-value:1.93E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:4.13|P-value:6.13E-6 ID:SGTC_518|Compound:1021-0119|FD-Score:3.75|P-value:3.70E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:4.43|P-value:1.39E-6 ID:SGTC_555|Compound:4296-0685|FD-Score:-3.45|P-value:1.42E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:4.30|P-value:2.75E-6 ID:SGTC_575|Compound:k072-0246|FD-Score:2.93|P-value:9.72E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:3.23|P-value:3.14E-4 ID:SGTC_658|Compound:3253-0951|FD-Score:4.22|P-value:3.93E-6 ID:SGTC_689|Compound:0833-0039|FD-Score:-3.74|P-value:4.07E-5 ID:SGTC_823|Compound:0417-1665|FD-Score:-3.07|P-value:6.21E-4 ID:SGTC_940|Compound:3232-1287|FD-Score:3.02|P-value:6.90E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:3.93|P-value:1.62E-5 ID:SGTC_1215|Compound:3852-0577|FD-Score:3.06|P-value:5.96E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.08|P-value:5.45E-4 ID:SGTC_1251|Compound:0490-5699|FD-Score:3.55|P-value:8.75E-5 ID:SGTC_1271|Compound:0832-2808|FD-Score:2.98|P-value:8.02E-4 ID:SGTC_1366|Compound:1683-0113|FD-Score:-3.63|P-value:6.65E-5 ID:SGTC_1508|Compound:1080-0625|FD-Score:3.76|P-value:3.41E-5 ID:SGTC_1547|Compound:harmine|FD-Score:-5.18|P-value:2.47E-8 ID:SGTC_1549|Compound:4'-methoxyflavonol|FD-Score:-3.52|P-value:1.05E-4 ID:SGTC_1571|Compound:st070080|FD-Score:-3.70|P-value:4.92E-5 ID:SGTC_1658|Compound:st012944|FD-Score:5.52|P-value:2.73E-9 ID:SGTC_1781|Compound:st038725|FD-Score:-2.96|P-value:9.30E-4 ID:SGTC_1782|Compound:st039423|FD-Score:-3.01|P-value:7.75E-4 ID:SGTC_1786|Compound:berberine|FD-Score:-5.94|P-value:1.98E-10 ID:SGTC_1787|Compound:fluconazole|FD-Score:-4.70|P-value:3.75E-7 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-3.53|P-value:9.98E-5 ID:SGTC_1866|Compound:st057638|FD-Score:2.94|P-value:9.22E-4 ID:SGTC_2021|Compound:4019055|FD-Score:4.10|P-value:7.37E-6 ID:SGTC_2175|Compound:5807670|FD-Score:3.25|P-value:2.91E-4 ID:SGTC_2358|Compound:9037157|FD-Score:3.22|P-value:3.24E-4 ID:SGTC_2415|Compound:st077333|FD-Score:3.77|P-value:3.37E-5 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:3.13|P-value:4.64E-4 ID:SGTC_2482|Compound:5981709|FD-Score:-3.49|P-value:1.18E-4 ID:SGTC_2484|Compound:5809818|FD-Score:-3.33|P-value:2.26E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.98|P-value:1.25E-5 ID:SGTC_2829|Compound:7992743|FD-Score:4.61|P-value:5.34E-7 ID:SGTC_2830|Compound:7992007|FD-Score:-4.20|P-value:5.02E-6 ID:SGTC_2831|Compound:7999036|FD-Score:4.65|P-value:4.35E-7 ID:SGTC_2834|Compound:9001755|FD-Score:3.32|P-value:2.21E-4 ID:SGTC_2935|Compound:9038016|FD-Score:3.29|P-value:2.44E-4 ID:SGTC_2990|Compound:9074105|FD-Score:-3.27|P-value:2.92E-4 ID:SGTC_2992|Compound:9074591|FD-Score:-3.29|P-value:2.64E-4 ID:SGTC_2997|Compound:9067075|FD-Score:5.13|P-value:2.92E-8 ID:SGTC_3005|Compound:9076496|FD-Score:-3.35|P-value:2.10E-4 ID:SGTC_3284|Compound:9108171|FD-Score:3.75|P-value:3.70E-5 ID:SGTC_3304|Compound:9125678|FD-Score:4.39|P-value:1.71E-6 ID:SGTC_3354|Compound:9151612|FD-Score:3.06|P-value:5.89E-4

Top fitness defect scores for YJR083C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1658 st012944 5.52 20.0 µM 2.73E-9 iron homeostasis 36 1.73E-8
2 SGTC_2997 9067075 5.13 71.4 µM 2.92E-8 unfolded protein response 27 1.46E-7
3 SGTC_2831 7999036 4.65 19.5 µM 4.35E-7 25 1.65E-6
4 SGTC_2829 7992743 4.61 58.4 µM 5.34E-7 23 1.99E-6
5 SGTC_528 3492-0019 4.43 172.0 µM 1.39E-6 calcium & mitochondrial duress 183 4.69E-6
6 SGTC_3304 9125678 4.39 46.1 µM 1.71E-6 34 5.66E-6
7 SGTC_568 1193-0097 4.30 7.5 µM 2.75E-6 ERAD & cell cycle 126 8.69E-6
8 SGTC_658 3253-0951 4.22 102.0 µM 3.93E-6 calcium & mitochondrial duress 53 1.20E-5
9 SGTC_453 enantio-paf c16 4.13 10.0 µM 6.13E-6 129 1.79E-5
10 SGTC_2021 4019055 4.10 173.0 µM 7.37E-6 endomembrane recycling 115 2.11E-5
11 SGTC_2688 kanamycin b 3.98 96.7 µM 1.25E-5 calcium & mitochondrial duress 358 3.40E-5
12 SGTC_1161 k007-0610 3.93 110.0 µM 1.62E-5 ERAD & cell cycle 123 4.28E-5
13 SGTC_2415 st077333 3.77 57.7 µM 3.37E-5 50 8.29E-5
14 SGTC_1508 1080-0625 3.76 72.9 µM 3.41E-5 23 8.38E-5
15 SGTC_518 1021-0119 3.75 62.6 µM 3.70E-5 ERG2 57 9.01E-5
16 SGTC_3284 9108171 3.75 64.9 µM 3.70E-5 30 9.02E-5
17 SGTC_1251 0490-5699 3.55 265.0 µM 8.75E-5 50 1.96E-4
18 SGTC_351 caspofungin 3.48 25.0 nM 1.16E-4 cell wall 135 2.53E-4
19 SGTC_2834 9001755 3.32 58.4 µM 2.21E-4 59 4.51E-4
20 SGTC_2935 9038016 3.29 30.0 µM 2.44E-4 44 4.93E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.196 2.71E-30 YOR034C-A_p YOR034C-A_p hom Putative protein of unknown function; identified by expression profiling and mass spectrometry
0.185 3.22E-27 YDR467C_d YDR467C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.181 5.01E-26 YLR408C_p BLS1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene
0.179 1.70E-25 YMR214W SCJ1 hom One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins
0.174 2.40E-24 YKL058W TOA2 het TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
0.173 6.93E-24 YBR258C SHG1 hom Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres
0.171 1.89E-23 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.158 2.71E-20 YDR459C PFA5 hom Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain
0.151 1.54E-18 YDL133W SRF1 hom Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients
0.147 1.08E-17 YLR380W CSR1 hom Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress
0.145 2.40E-17 YOR086C TCB1 hom Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.143 1.06E-16 YKL061W_p BLI1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome
0.142 1.66E-16 YBL022C PIM1 hom ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria
0.141 2.80E-16 YDR309C GIC2 hom Redundant rho-like GTPase Cdc42p effector; homolog of Gic1p; involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region
0.141 2.85E-16 YMR230W-A_p YMR230W-A_p hom Putative protein of unknown function