YJR131W / MNS1

Alpha-1,2-mannosidase involved in ER-associated protein degradation (ERAD); catalyzes the removal of one mannose residue from a glycosylated protein, converting the modification from Man9GlcNAc to Man8GlcNAc; catalyzes the last step in glycoprotein maturation in the ER and is critical for ER protein degradation

Zygosity: Homozygous strain
fixedexpanded
Profile for YJR131W / MNS1

Click on Significant Values for Screen Details ID:SGTC_131|Compound:0141-0277|FD-Score:-3.14|P-value:9.04E-4 ID:SGTC_142|Compound:0335-0849|FD-Score:4.54|P-value:3.33E-6 ID:SGTC_146|Compound:3448-8650|FD-Score:-6.38|P-value:1.17E-10 ID:SGTC_346|Compound:4513-0042|FD-Score:4.30|P-value:1.02E-5 ID:SGTC_375|Compound:3970-0961|FD-Score:3.36|P-value:4.30E-4 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:-4.00|P-value:3.56E-5 ID:SGTC_450|Compound:aminopyrene|FD-Score:3.51|P-value:2.53E-4 ID:SGTC_493|Compound:zm226600|FD-Score:5.46|P-value:3.06E-8 ID:SGTC_514|Compound:4466-0038|FD-Score:3.95|P-value:4.52E-5 ID:SGTC_669|Compound:0108-0021|FD-Score:3.88|P-value:5.88E-5 ID:SGTC_670|Compound:4204-0025|FD-Score:4.17|P-value:1.74E-5 ID:SGTC_958|Compound:1222-0007|FD-Score:4.61|P-value:2.38E-6 ID:SGTC_996|Compound:3970-0799|FD-Score:3.61|P-value:1.72E-4 ID:SGTC_1052|Compound:3253-3998|FD-Score:-3.42|P-value:3.40E-4 ID:SGTC_1380|Compound:2291-0730|FD-Score:-3.46|P-value:2.90E-4 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:-3.18|P-value:7.80E-4 ID:SGTC_1538|Compound:2',3',6-trimethoxyflavanone|FD-Score:3.78|P-value:8.80E-5 ID:SGTC_1676|Compound:st018489|FD-Score:-3.60|P-value:1.74E-4 ID:SGTC_1681|Compound:tripelennamine|FD-Score:-3.85|P-value:6.56E-5 ID:SGTC_1725|Compound:st036284|FD-Score:3.85|P-value:6.83E-5 ID:SGTC_1914|Compound:st062367|FD-Score:-3.15|P-value:8.82E-4 ID:SGTC_1924|Compound:st058459|FD-Score:3.29|P-value:5.62E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.26|P-value:5.95E-4 ID:SGTC_2237|Compound:6667747|FD-Score:4.51|P-value:3.81E-6 ID:SGTC_2238|Compound:6671620|FD-Score:-3.52|P-value:2.32E-4 ID:SGTC_2239|Compound:6685329|FD-Score:4.91|P-value:5.54E-7 ID:SGTC_2330|Compound:7979373|FD-Score:-4.32|P-value:8.78E-6 ID:SGTC_2331|Compound:7980721|FD-Score:3.66|P-value:1.44E-4 ID:SGTC_2337|Compound:9009472|FD-Score:6.53|P-value:4.54E-11 ID:SGTC_2397|Compound:5161689|FD-Score:-3.22|P-value:6.96E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.23|P-value:6.79E-4 ID:SGTC_2625|Compound:euphol|FD-Score:3.45|P-value:3.12E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:-6.43|P-value:8.71E-11 ID:SGTC_2638|Compound:epoxomicin|FD-Score:3.21|P-value:7.43E-4 ID:SGTC_2803|Compound:7707263|FD-Score:4.57|P-value:2.89E-6 ID:SGTC_2896|Compound:9052656|FD-Score:-3.20|P-value:7.40E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:-3.14|P-value:9.04E-4 ID:SGTC_142|Compound:0335-0849|FD-Score:4.54|P-value:3.33E-6 ID:SGTC_146|Compound:3448-8650|FD-Score:-6.38|P-value:1.17E-10 ID:SGTC_346|Compound:4513-0042|FD-Score:4.30|P-value:1.02E-5 ID:SGTC_375|Compound:3970-0961|FD-Score:3.36|P-value:4.30E-4 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:-4.00|P-value:3.56E-5 ID:SGTC_450|Compound:aminopyrene|FD-Score:3.51|P-value:2.53E-4 ID:SGTC_493|Compound:zm226600|FD-Score:5.46|P-value:3.06E-8 ID:SGTC_514|Compound:4466-0038|FD-Score:3.95|P-value:4.52E-5 ID:SGTC_669|Compound:0108-0021|FD-Score:3.88|P-value:5.88E-5 ID:SGTC_670|Compound:4204-0025|FD-Score:4.17|P-value:1.74E-5 ID:SGTC_958|Compound:1222-0007|FD-Score:4.61|P-value:2.38E-6 ID:SGTC_996|Compound:3970-0799|FD-Score:3.61|P-value:1.72E-4 ID:SGTC_1052|Compound:3253-3998|FD-Score:-3.42|P-value:3.40E-4 ID:SGTC_1380|Compound:2291-0730|FD-Score:-3.46|P-value:2.90E-4 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:-3.18|P-value:7.80E-4 ID:SGTC_1538|Compound:2',3',6-trimethoxyflavanone|FD-Score:3.78|P-value:8.80E-5 ID:SGTC_1676|Compound:st018489|FD-Score:-3.60|P-value:1.74E-4 ID:SGTC_1681|Compound:tripelennamine|FD-Score:-3.85|P-value:6.56E-5 ID:SGTC_1725|Compound:st036284|FD-Score:3.85|P-value:6.83E-5 ID:SGTC_1914|Compound:st062367|FD-Score:-3.15|P-value:8.82E-4 ID:SGTC_1924|Compound:st058459|FD-Score:3.29|P-value:5.62E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.26|P-value:5.95E-4 ID:SGTC_2237|Compound:6667747|FD-Score:4.51|P-value:3.81E-6 ID:SGTC_2238|Compound:6671620|FD-Score:-3.52|P-value:2.32E-4 ID:SGTC_2239|Compound:6685329|FD-Score:4.91|P-value:5.54E-7 ID:SGTC_2330|Compound:7979373|FD-Score:-4.32|P-value:8.78E-6 ID:SGTC_2331|Compound:7980721|FD-Score:3.66|P-value:1.44E-4 ID:SGTC_2337|Compound:9009472|FD-Score:6.53|P-value:4.54E-11 ID:SGTC_2397|Compound:5161689|FD-Score:-3.22|P-value:6.96E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.23|P-value:6.79E-4 ID:SGTC_2625|Compound:euphol|FD-Score:3.45|P-value:3.12E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:-6.43|P-value:8.71E-11 ID:SGTC_2638|Compound:epoxomicin|FD-Score:3.21|P-value:7.43E-4 ID:SGTC_2803|Compound:7707263|FD-Score:4.57|P-value:2.89E-6 ID:SGTC_2896|Compound:9052656|FD-Score:-3.20|P-value:7.40E-4

Top fitness defect scores for YJR131W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2337 9009472 6.53 200.0 µM 4.54E-11 3 3.20E-11
2 SGTC_493 zm226600 5.46 134.0 µM 3.06E-8 mitochondrial processes 4 2.38E-8
3 SGTC_2239 6685329 4.91 187.6 µM 5.54E-7 9 4.50E-7
4 SGTC_958 1222-0007 4.61 33.0 µM 2.38E-6 16 1.98E-6
5 SGTC_2803 7707263 4.57 71.4 µM 2.89E-6 20 2.41E-6
6 SGTC_142 0335-0849 4.54 62.7 µM 3.33E-6 ubiquinone biosynthesis & proteasome 102 2.78E-6
7 SGTC_2237 6667747 4.51 47.2 µM 3.81E-6 10 3.19E-6
8 SGTC_346 4513-0042 4.30 7.2 µM 1.02E-5 azole & statin 33 8.68E-6
9 SGTC_670 4204-0025 4.17 119.0 µM 1.74E-5 copper-dependent oxidative stress 27 1.49E-5
10 SGTC_514 4466-0038 3.95 2.6 nM 4.52E-5 cell wall 157 3.93E-5
11 SGTC_669 0108-0021 3.88 26.2 µM 5.88E-5 fatty acid desaturase (OLE1) 86 5.13E-5
12 SGTC_1725 st036284 3.85 20.6 µM 6.83E-5 27 5.97E-5
13 SGTC_1538 2',3',6-trimethoxyflavanone 3.78 63.6 µM 8.80E-5 18 7.72E-5
14 SGTC_2331 7980721 3.66 200.0 µM 1.44E-4 36 1.28E-4
15 SGTC_996 3970-0799 3.61 1.8 µM 1.72E-4 102 1.52E-4
16 SGTC_450 aminopyrene 3.51 156.0 nM 2.53E-4 48 2.26E-4
17 SGTC_2625 euphol 3.45 100.0 µM 3.12E-4 49 2.79E-4
18 SGTC_375 3970-0961 3.36 9.3 µM 4.30E-4 tubulin folding & SWR complex 39 3.87E-4
19 SGTC_1924 st058459 3.29 75.1 µM 5.62E-4 27 5.08E-4
20 SGTC_2567 aureobasidin a 3.23 60.0 nM 6.79E-4 134 6.14E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.161 1.01E-20 YDR242W AMD2 hom Putative amidase
0.160 8.76E-21 YOR037W CYC2 hom Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
0.150 1.82E-18 YGR050C_p YGR050C_p hom Protein of unknown function; mRNA identified as translated by ribosome profiling data
0.143 7.72E-17 YBR083W TEC1 hom Transcription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member
0.134 5.37E-15 YDR339C FCF1 het Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p
0.128 9.92E-14 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.122 1.46E-12 YKL220C FRE2 hom Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels
0.120 3.01E-12 YDR107C TMN2 hom Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments
0.118 6.91E-12 YMR121C RPL15B hom Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
0.117 1.06E-11 YBL066C SEF1 hom Putative transcription factor, has homolog in Kluyveromyces lactis
0.112 9.45E-11 YJL193W_p YJL193W_p hom Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect
0.111 1.05E-10 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.110 1.42E-10 YKL004W AUR1 het Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.110 1.45E-10 YKL083W_d YKL083W_d het Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14
0.109 2.25E-10 YKL010C UFD4 hom Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress