YJR145C / RPS4A

Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YJR145C / RPS4A

Click on Significant Values for Screen Details ID:SGTC_48|Compound:0382-0018|FD-Score:-3.67|P-value:8.17E-5 ID:SGTC_53|Compound:0973-0023|FD-Score:-4.45|P-value:2.34E-6 ID:SGTC_69|Compound:0096-0274|FD-Score:-3.30|P-value:3.41E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:3.03|P-value:8.87E-4 ID:SGTC_368|Compound:fentichlor|FD-Score:3.23|P-value:4.31E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-3.23|P-value:4.53E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:6.24|P-value:6.47E-11 ID:SGTC_634|Compound:1611-4317|FD-Score:3.36|P-value:2.72E-4 ID:SGTC_691|Compound:1548-0686|FD-Score:3.43|P-value:2.01E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:-3.14|P-value:6.30E-4 ID:SGTC_766|Compound:1391-0721|FD-Score:-3.42|P-value:2.14E-4 ID:SGTC_771|Compound:0250-0039|FD-Score:-3.28|P-value:3.66E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:5.67|P-value:2.65E-9 ID:SGTC_806|Compound:3966-4455|FD-Score:5.03|P-value:1.12E-7 ID:SGTC_830|Compound:4076-0286|FD-Score:3.04|P-value:8.57E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:4.12|P-value:1.08E-5 ID:SGTC_899|Compound:1083-0020|FD-Score:3.36|P-value:2.64E-4 ID:SGTC_900|Compound:1112-0045|FD-Score:5.45|P-value:9.60E-9 ID:SGTC_1157|Compound:4092-0394|FD-Score:3.17|P-value:5.49E-4 ID:SGTC_1170|Compound:0310-0127|FD-Score:-3.18|P-value:5.31E-4 ID:SGTC_1308|Compound:1187-1617|FD-Score:3.15|P-value:5.82E-4 ID:SGTC_1362|Compound:1611-0397|FD-Score:4.02|P-value:1.70E-5 ID:SGTC_1581|Compound:himbacine|FD-Score:3.44|P-value:1.94E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-5.03|P-value:1.11E-7 ID:SGTC_1764|Compound:st044502|FD-Score:5.12|P-value:6.85E-8 ID:SGTC_1768|Compound:st044301|FD-Score:3.01|P-value:9.54E-4 ID:SGTC_1821|Compound:st053465|FD-Score:3.18|P-value:5.28E-4 ID:SGTC_1862|Compound:st057513|FD-Score:3.49|P-value:1.59E-4 ID:SGTC_1921|Compound:5740136|FD-Score:3.33|P-value:2.95E-4 ID:SGTC_1957|Compound:st077035|FD-Score:-3.25|P-value:4.17E-4 ID:SGTC_2006|Compound:4011487|FD-Score:-4.36|P-value:3.63E-6 ID:SGTC_2048|Compound:5246248|FD-Score:-3.10|P-value:7.21E-4 ID:SGTC_2243|Compound:6634756|FD-Score:-3.09|P-value:7.33E-4 ID:SGTC_2337|Compound:9009472|FD-Score:3.16|P-value:5.62E-4 ID:SGTC_2486|Compound:5283202|FD-Score:-3.36|P-value:2.76E-4 ID:SGTC_2543|Compound:pimpinellin|FD-Score:3.40|P-value:2.32E-4 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:-3.30|P-value:3.43E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:-3.10|P-value:7.04E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.41|P-value:1.27E-8 ID:SGTC_2777|Compound:climbazole|FD-Score:-3.02|P-value:9.52E-4 ID:SGTC_2908|Compound:7930301|FD-Score:3.00|P-value:9.87E-4 ID:SGTC_2937|Compound:9044753|FD-Score:3.85|P-value:3.57E-5 ID:SGTC_48|Compound:0382-0018|FD-Score:-3.67|P-value:8.17E-5 ID:SGTC_53|Compound:0973-0023|FD-Score:-4.45|P-value:2.34E-6 ID:SGTC_69|Compound:0096-0274|FD-Score:-3.30|P-value:3.41E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:3.03|P-value:8.87E-4 ID:SGTC_368|Compound:fentichlor|FD-Score:3.23|P-value:4.31E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-3.23|P-value:4.53E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:6.24|P-value:6.47E-11 ID:SGTC_634|Compound:1611-4317|FD-Score:3.36|P-value:2.72E-4 ID:SGTC_691|Compound:1548-0686|FD-Score:3.43|P-value:2.01E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:-3.14|P-value:6.30E-4 ID:SGTC_766|Compound:1391-0721|FD-Score:-3.42|P-value:2.14E-4 ID:SGTC_771|Compound:0250-0039|FD-Score:-3.28|P-value:3.66E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:5.67|P-value:2.65E-9 ID:SGTC_806|Compound:3966-4455|FD-Score:5.03|P-value:1.12E-7 ID:SGTC_830|Compound:4076-0286|FD-Score:3.04|P-value:8.57E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:4.12|P-value:1.08E-5 ID:SGTC_899|Compound:1083-0020|FD-Score:3.36|P-value:2.64E-4 ID:SGTC_900|Compound:1112-0045|FD-Score:5.45|P-value:9.60E-9 ID:SGTC_1157|Compound:4092-0394|FD-Score:3.17|P-value:5.49E-4 ID:SGTC_1170|Compound:0310-0127|FD-Score:-3.18|P-value:5.31E-4 ID:SGTC_1308|Compound:1187-1617|FD-Score:3.15|P-value:5.82E-4 ID:SGTC_1362|Compound:1611-0397|FD-Score:4.02|P-value:1.70E-5 ID:SGTC_1581|Compound:himbacine|FD-Score:3.44|P-value:1.94E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-5.03|P-value:1.11E-7 ID:SGTC_1764|Compound:st044502|FD-Score:5.12|P-value:6.85E-8 ID:SGTC_1768|Compound:st044301|FD-Score:3.01|P-value:9.54E-4 ID:SGTC_1821|Compound:st053465|FD-Score:3.18|P-value:5.28E-4 ID:SGTC_1862|Compound:st057513|FD-Score:3.49|P-value:1.59E-4 ID:SGTC_1921|Compound:5740136|FD-Score:3.33|P-value:2.95E-4 ID:SGTC_1957|Compound:st077035|FD-Score:-3.25|P-value:4.17E-4 ID:SGTC_2006|Compound:4011487|FD-Score:-4.36|P-value:3.63E-6 ID:SGTC_2048|Compound:5246248|FD-Score:-3.10|P-value:7.21E-4 ID:SGTC_2243|Compound:6634756|FD-Score:-3.09|P-value:7.33E-4 ID:SGTC_2337|Compound:9009472|FD-Score:3.16|P-value:5.62E-4 ID:SGTC_2486|Compound:5283202|FD-Score:-3.36|P-value:2.76E-4 ID:SGTC_2543|Compound:pimpinellin|FD-Score:3.40|P-value:2.32E-4 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:-3.30|P-value:3.43E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:-3.10|P-value:7.04E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.41|P-value:1.27E-8 ID:SGTC_2777|Compound:climbazole|FD-Score:-3.02|P-value:9.52E-4 ID:SGTC_2908|Compound:7930301|FD-Score:3.00|P-value:9.87E-4 ID:SGTC_2937|Compound:9044753|FD-Score:3.85|P-value:3.57E-5

Top fitness defect scores for YJR145C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_625 k213-0058 6.24 121.0 µM 6.47E-11 translation 28 2.18E-10
2 SGTC_800 3448-7362 5.67 155.0 µM 2.65E-9 translation 61 7.27E-9
3 SGTC_900 1112-0045 5.45 141.0 µM 9.60E-9 NEO1 15 2.45E-8
4 SGTC_2688 kanamycin b 5.41 96.7 µM 1.27E-8 calcium & mitochondrial duress 203 3.19E-8
5 SGTC_1764 st044502 5.12 93.5 µM 6.85E-8 16 1.57E-7
6 SGTC_806 3966-4455 5.03 5.9 µM 1.12E-7 6 2.50E-7
7 SGTC_832 4083-0019 4.12 164.0 µM 1.08E-5 translation 85 1.87E-5
8 SGTC_1362 1611-0397 4.02 101.0 µM 1.70E-5 19 2.88E-5
9 SGTC_2937 9044753 3.85 11.3 µM 3.57E-5 60S ribosome export 37 5.80E-5
10 SGTC_1862 st057513 3.49 3.8 µM 1.59E-4 25 2.37E-4
11 SGTC_1581 himbacine 3.44 57.9 µM 1.94E-4 Golgi 61 2.88E-4
12 SGTC_691 1548-0686 3.43 185.0 µM 2.01E-4 60 2.97E-4
13 SGTC_2543 pimpinellin 3.40 60.7 µM 2.32E-4 Golgi 35 3.41E-4
14 SGTC_899 1083-0020 3.36 478.0 µM 2.64E-4 42 3.85E-4
15 SGTC_634 1611-4317 3.36 11.2 µM 2.72E-4 ERG2 33 3.96E-4
16 SGTC_1921 5740136 3.33 50.0 µM 2.95E-4 TRP & mitochondrial translation 86 4.27E-4
17 SGTC_368 fentichlor 3.23 4.6 µM 4.31E-4 50 6.11E-4
18 SGTC_1821 st053465 3.18 65.6 µM 5.28E-4 26 7.42E-4
19 SGTC_1157 4092-0394 3.17 27.6 µM 5.49E-4 55 7.69E-4
20 SGTC_2337 9009472 3.16 200.0 µM 5.62E-4 68 7.86E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.210 1.15E-34 YLR048W RPS0B hom Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.191 8.43E-29 YEL054C RPL12A hom Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication
0.187 7.06E-28 YGR162W TIF4631 hom Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication
0.182 2.23E-26 YOR085W OST3 hom Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.178 2.61E-25 YPL249C-A RPL36B hom Ribosomal 60S subunit protein L36B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36B has a paralog, RPL36A, that arose from the whole genome duplication
0.175 2.06E-24 YLR287C-A RPS30A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication
0.174 2.59E-24 YGR214W RPS0A hom Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication;
0.168 9.28E-23 YJL188C_d BUD19_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay
0.167 1.65E-22 YBR191W RPL21A hom Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication
0.161 6.95E-21 YEL042W GDA1 hom Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate
0.157 7.03E-20 YDR417C_d YDR417C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W
0.156 8.72E-20 YJL189W RPL39 hom Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog
0.147 9.25E-18 YDR140W MTQ2 hom S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC
0.147 1.48E-17 YJL069C UTP18 het Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data
0.146 1.87E-17 YGL246C RAI1 hom Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z