YKL010C / UFD4

Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL010C / UFD4

Click on Significant Values for Screen Details ID:SGTC_310|Compound:1273-0058|FD-Score:-3.41|P-value:2.64E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.49|P-value:1.91E-4 ID:SGTC_493|Compound:zm226600|FD-Score:6.39|P-value:5.10E-11 ID:SGTC_494|Compound:Boc-Gly-Val-Val-CHO|FD-Score:-4.97|P-value:2.18E-7 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:-4.44|P-value:3.29E-6 ID:SGTC_504|Compound:lovastatin|FD-Score:-6.14|P-value:2.23E-10 ID:SGTC_508|Compound:roscovitine|FD-Score:-4.19|P-value:1.01E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.06|P-value:9.12E-4 ID:SGTC_912|Compound:3474-0017|FD-Score:3.25|P-value:5.09E-4 ID:SGTC_914|Compound:3820-6779|FD-Score:-3.19|P-value:5.79E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:3.11|P-value:8.36E-4 ID:SGTC_947|Compound:3447-0010|FD-Score:-5.11|P-value:1.04E-7 ID:SGTC_948|Compound:3448-0295|FD-Score:-3.05|P-value:9.69E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:3.31|P-value:4.18E-4 ID:SGTC_954|Compound:1189-0853|FD-Score:-6.42|P-value:3.67E-11 ID:SGTC_996|Compound:3970-0799|FD-Score:5.28|P-value:4.71E-8 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.75|P-value:6.85E-5 ID:SGTC_1133|Compound:3473-0247|FD-Score:3.40|P-value:2.99E-4 ID:SGTC_1164|Compound:k060-0068|FD-Score:-4.01|P-value:2.32E-5 ID:SGTC_1248|Compound:0469-0687|FD-Score:-3.27|P-value:4.34E-4 ID:SGTC_1303|Compound:1160-0052|FD-Score:-4.22|P-value:8.88E-6 ID:SGTC_1305|Compound:1181-0477|FD-Score:3.15|P-value:7.35E-4 ID:SGTC_1309|Compound:pyrazolanthrone|FD-Score:3.70|P-value:9.39E-5 ID:SGTC_1753|Compound:st043925|FD-Score:3.59|P-value:1.42E-4 ID:SGTC_1756|Compound:st041558|FD-Score:3.43|P-value:2.65E-4 ID:SGTC_1889|Compound:st059080|FD-Score:3.21|P-value:5.95E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.31|P-value:3.85E-4 ID:SGTC_2111|Compound:5486399|FD-Score:7.12|P-value:2.84E-13 ID:SGTC_2341|Compound:7987486|FD-Score:3.55|P-value:1.69E-4 ID:SGTC_2378|Compound:9074213|FD-Score:-3.40|P-value:2.75E-4 ID:SGTC_2513|Compound:baicalein|FD-Score:-3.31|P-value:3.75E-4 ID:SGTC_2909|Compound:7941780|FD-Score:3.25|P-value:5.13E-4 ID:SGTC_2971|Compound:9092186|FD-Score:-4.71|P-value:8.53E-7 ID:SGTC_3112|Compound:9123419|FD-Score:-3.14|P-value:7.05E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.41|P-value:2.64E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.49|P-value:1.91E-4 ID:SGTC_493|Compound:zm226600|FD-Score:6.39|P-value:5.10E-11 ID:SGTC_494|Compound:Boc-Gly-Val-Val-CHO|FD-Score:-4.97|P-value:2.18E-7 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:-4.44|P-value:3.29E-6 ID:SGTC_504|Compound:lovastatin|FD-Score:-6.14|P-value:2.23E-10 ID:SGTC_508|Compound:roscovitine|FD-Score:-4.19|P-value:1.01E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.06|P-value:9.12E-4 ID:SGTC_912|Compound:3474-0017|FD-Score:3.25|P-value:5.09E-4 ID:SGTC_914|Compound:3820-6779|FD-Score:-3.19|P-value:5.79E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:3.11|P-value:8.36E-4 ID:SGTC_947|Compound:3447-0010|FD-Score:-5.11|P-value:1.04E-7 ID:SGTC_948|Compound:3448-0295|FD-Score:-3.05|P-value:9.69E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:3.31|P-value:4.18E-4 ID:SGTC_954|Compound:1189-0853|FD-Score:-6.42|P-value:3.67E-11 ID:SGTC_996|Compound:3970-0799|FD-Score:5.28|P-value:4.71E-8 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.75|P-value:6.85E-5 ID:SGTC_1133|Compound:3473-0247|FD-Score:3.40|P-value:2.99E-4 ID:SGTC_1164|Compound:k060-0068|FD-Score:-4.01|P-value:2.32E-5 ID:SGTC_1248|Compound:0469-0687|FD-Score:-3.27|P-value:4.34E-4 ID:SGTC_1303|Compound:1160-0052|FD-Score:-4.22|P-value:8.88E-6 ID:SGTC_1305|Compound:1181-0477|FD-Score:3.15|P-value:7.35E-4 ID:SGTC_1309|Compound:pyrazolanthrone|FD-Score:3.70|P-value:9.39E-5 ID:SGTC_1753|Compound:st043925|FD-Score:3.59|P-value:1.42E-4 ID:SGTC_1756|Compound:st041558|FD-Score:3.43|P-value:2.65E-4 ID:SGTC_1889|Compound:st059080|FD-Score:3.21|P-value:5.95E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.31|P-value:3.85E-4 ID:SGTC_2111|Compound:5486399|FD-Score:7.12|P-value:2.84E-13 ID:SGTC_2341|Compound:7987486|FD-Score:3.55|P-value:1.69E-4 ID:SGTC_2378|Compound:9074213|FD-Score:-3.40|P-value:2.75E-4 ID:SGTC_2513|Compound:baicalein|FD-Score:-3.31|P-value:3.75E-4 ID:SGTC_2909|Compound:7941780|FD-Score:3.25|P-value:5.13E-4 ID:SGTC_2971|Compound:9092186|FD-Score:-4.71|P-value:8.53E-7 ID:SGTC_3112|Compound:9123419|FD-Score:-3.14|P-value:7.05E-4

Top fitness defect scores for YKL010C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2111 5486399 7.12 200.0 µM 2.84E-13 4 5.21E-13
2 SGTC_493 zm226600 6.39 134.0 µM 5.10E-11 mitochondrial processes 2 8.26E-11
3 SGTC_996 3970-0799 5.28 1.8 µM 4.71E-8 47 6.50E-8
4 SGTC_1309 pyrazolanthrone 3.70 13.4 µM 9.39E-5 27 1.09E-4
5 SGTC_1753 st043925 3.59 64.0 µM 1.42E-4 13 1.63E-4
6 SGTC_2341 7987486 3.55 176.7 µM 1.69E-4 30 1.93E-4
7 SGTC_1756 st041558 3.43 35.5 µM 2.65E-4 17 3.01E-4
8 SGTC_1133 3473-0247 3.40 50.0 µM 2.99E-4 RSC complex & mRNA processing 76 3.38E-4
9 SGTC_953 1188-0045 3.31 9.7 µM 4.18E-4 60S ribosome export 51 4.68E-4
10 SGTC_912 3474-0017 3.25 2.2 µM 5.09E-4 47 5.69E-4
11 SGTC_2909 7941780 3.25 10.5 µM 5.13E-4 48 5.73E-4
12 SGTC_1889 st059080 3.21 5.2 µM 5.95E-4 49 6.62E-4
13 SGTC_1305 1181-0477 3.15 144.0 µM 7.35E-4 43 8.15E-4
14 SGTC_916 4466-0038 3.11 42.0 nM 8.36E-4 cell wall 128 9.24E-4
15 SGTC_1332 1480-0653 3.05 17.5 µM 0.00104 TSC3-RPN4 47 0.00115
16 SGTC_3323 9138564 3.04 53.9 µM 0.00109 56 0.00120
17 SGTC_442 cape 3.02 100.0 µM 0.00115 34 0.00126
18 SGTC_2316 5844804 2.98 197.4 µM 0.00133 96 0.00146
19 SGTC_773 4358-1457 2.97 44.6 µM 0.00136 91 0.00149
20 SGTC_1525 8-methoxypsoralen 2.94 92.5 µM 0.00151 DNA damage response 92 0.00165

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.223 4.88E-39 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.210 1.21E-34 YOR037W CYC2 hom Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
0.193 2.88E-29 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.188 4.36E-28 YBR084W MIS1 hom Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase
0.184 6.74E-27 YGL096W TOS8 hom Homeodomain-containing protein and putative transcription factor; found associated with chromatin; target of SBF transcription factor; induced during meiosis and under cell-damaging conditions; TOS8 has a paralog, CUP9, that arose from the whole genome duplication
0.169 1.05E-22 YDR242W AMD2 hom Putative amidase
0.165 9.18E-22 YGR025W_d YGR025W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.163 2.18E-21 YMR104C YPK2 hom Protein kinase with similarity to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK; YPK2 has a paralog, YPK1, that arose from the whole genome duplication
0.157 6.05E-20 YDL099W BUG1 hom Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.151 1.87E-18 YGR019W UGA1 hom Gamma-aminobutyrate (GABA) transaminase; also known as 4-aminobutyrate aminotransferase; involved in the 4-aminobutyrate and glutamate degradation pathways; required for normal oxidative stress tolerance and nitrogen utilization; protein abundance increases in response to DNA replication stress
0.149 4.18E-18 YGR109C CLB6 hom B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1
0.147 1.37E-17 YJR094C IME1 hom Master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p
0.143 1.06E-16 YGL205W POX1 hom Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix
0.135 4.33E-15 YMR173W DDR48 hom DNA damage-responsive protein; expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS; protein abundance increases in response to DNA replication stress
0.131 2.18E-14 YGL040C HEM2 het Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus