YKL015W / PUT3

Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences as a dimer and undergoes a conformational change to form the active state; differentially phosphorylated in the presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL015W / PUT3

Click on Significant Values for Screen Details ID:SGTC_307|Compound:1501-1223|FD-Score:4.09|P-value:3.01E-6 ID:SGTC_422|Compound:cantharidin|FD-Score:3.74|P-value:1.82E-5 ID:SGTC_429|Compound:1112-0049|FD-Score:4.25|P-value:1.28E-6 ID:SGTC_524|Compound:r073-0010|FD-Score:-3.29|P-value:1.30E-4 ID:SGTC_527|Compound:4476-4215|FD-Score:-4.07|P-value:3.09E-6 ID:SGTC_539|Compound:4859-0462|FD-Score:2.93|P-value:6.13E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:3.15|P-value:2.54E-4 ID:SGTC_930|Compound:2810-3379|FD-Score:2.88|P-value:7.34E-4 ID:SGTC_1006|Compound:4099-6547|FD-Score:3.14|P-value:2.62E-4 ID:SGTC_1080|Compound:nisoldipine|FD-Score:3.10|P-value:3.10E-4 ID:SGTC_1126|Compound:isoteolin|FD-Score:-4.14|P-value:2.15E-6 ID:SGTC_1138|Compound:3761-0006|FD-Score:4.40|P-value:5.79E-7 ID:SGTC_1139|Compound:k015-0024|FD-Score:4.14|P-value:2.32E-6 ID:SGTC_1293|Compound:1000-1278|FD-Score:-2.99|P-value:4.42E-4 ID:SGTC_1610|Compound:st001799|FD-Score:-3.98|P-value:4.90E-6 ID:SGTC_1719|Compound:st032227|FD-Score:5.06|P-value:1.11E-8 ID:SGTC_1773|Compound:st048542|FD-Score:3.00|P-value:4.66E-4 ID:SGTC_2008|Compound:4012253|FD-Score:-2.90|P-value:6.46E-4 ID:SGTC_2239|Compound:6685329|FD-Score:4.56|P-value:2.34E-7 ID:SGTC_2482|Compound:5981709|FD-Score:2.84|P-value:8.52E-4 ID:SGTC_2639|Compound:lactacystin|FD-Score:3.15|P-value:2.47E-4 ID:SGTC_2767|Compound:sibutramine|FD-Score:5.95|P-value:2.30E-11 ID:SGTC_2785|Compound:7756463|FD-Score:-2.84|P-value:8.16E-4 ID:SGTC_2813|Compound:7971799|FD-Score:-3.16|P-value:2.26E-4 ID:SGTC_2923|Compound:7992654|FD-Score:-3.07|P-value:3.20E-4 ID:SGTC_3202|Compound:9113761|FD-Score:4.24|P-value:1.39E-6 ID:SGTC_3209|Compound:9113616|FD-Score:3.87|P-value:9.42E-6 ID:SGTC_3226|Compound:9131256|FD-Score:3.49|P-value:5.80E-5 ID:SGTC_3257|Compound:9139206|FD-Score:3.30|P-value:1.35E-4 ID:SGTC_307|Compound:1501-1223|FD-Score:4.09|P-value:3.01E-6 ID:SGTC_422|Compound:cantharidin|FD-Score:3.74|P-value:1.82E-5 ID:SGTC_429|Compound:1112-0049|FD-Score:4.25|P-value:1.28E-6 ID:SGTC_524|Compound:r073-0010|FD-Score:-3.29|P-value:1.30E-4 ID:SGTC_527|Compound:4476-4215|FD-Score:-4.07|P-value:3.09E-6 ID:SGTC_539|Compound:4859-0462|FD-Score:2.93|P-value:6.13E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:3.15|P-value:2.54E-4 ID:SGTC_930|Compound:2810-3379|FD-Score:2.88|P-value:7.34E-4 ID:SGTC_1006|Compound:4099-6547|FD-Score:3.14|P-value:2.62E-4 ID:SGTC_1080|Compound:nisoldipine|FD-Score:3.10|P-value:3.10E-4 ID:SGTC_1126|Compound:isoteolin|FD-Score:-4.14|P-value:2.15E-6 ID:SGTC_1138|Compound:3761-0006|FD-Score:4.40|P-value:5.79E-7 ID:SGTC_1139|Compound:k015-0024|FD-Score:4.14|P-value:2.32E-6 ID:SGTC_1293|Compound:1000-1278|FD-Score:-2.99|P-value:4.42E-4 ID:SGTC_1610|Compound:st001799|FD-Score:-3.98|P-value:4.90E-6 ID:SGTC_1719|Compound:st032227|FD-Score:5.06|P-value:1.11E-8 ID:SGTC_1773|Compound:st048542|FD-Score:3.00|P-value:4.66E-4 ID:SGTC_2008|Compound:4012253|FD-Score:-2.90|P-value:6.46E-4 ID:SGTC_2239|Compound:6685329|FD-Score:4.56|P-value:2.34E-7 ID:SGTC_2482|Compound:5981709|FD-Score:2.84|P-value:8.52E-4 ID:SGTC_2639|Compound:lactacystin|FD-Score:3.15|P-value:2.47E-4 ID:SGTC_2767|Compound:sibutramine|FD-Score:5.95|P-value:2.30E-11 ID:SGTC_2785|Compound:7756463|FD-Score:-2.84|P-value:8.16E-4 ID:SGTC_2813|Compound:7971799|FD-Score:-3.16|P-value:2.26E-4 ID:SGTC_2923|Compound:7992654|FD-Score:-3.07|P-value:3.20E-4 ID:SGTC_3202|Compound:9113761|FD-Score:4.24|P-value:1.39E-6 ID:SGTC_3209|Compound:9113616|FD-Score:3.87|P-value:9.42E-6 ID:SGTC_3226|Compound:9131256|FD-Score:3.49|P-value:5.80E-5 ID:SGTC_3257|Compound:9139206|FD-Score:3.30|P-value:1.35E-4

Top fitness defect scores for YKL015W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2767 sibutramine 5.95 71.5 µM 2.30E-11 3 1.33E-9
2 SGTC_1719 st032227 5.06 49.2 µM 1.11E-8 9 2.13E-7
3 SGTC_2239 6685329 4.56 187.6 µM 2.34E-7 13 2.60E-6
4 SGTC_1138 3761-0006 4.40 1.0 µM 5.79E-7 1 5.48E-6
5 SGTC_429 1112-0049 4.25 25.5 µM 1.28E-6 DNA damage response 28 1.05E-5
6 SGTC_3202 9113761 4.24 49.5 µM 1.39E-6 38 1.12E-5
7 SGTC_1139 k015-0024 4.14 46.9 µM 2.32E-6 PDR1 11 1.72E-5
8 SGTC_307 1501-1223 4.09 22.3 µM 3.01E-6 11 2.13E-5
9 SGTC_3209 9113616 3.87 49.5 µM 9.42E-6 27 5.44E-5
10 SGTC_422 cantharidin 3.74 155.0 µM 1.82E-5 cell wall signaling 67 9.36E-5
11 SGTC_3226 9131256 3.49 49.5 µM 5.80E-5 2 2.44E-4
12 SGTC_3257 9139206 3.30 33.0 µM 1.35E-4 16 4.90E-4
13 SGTC_2639 lactacystin 3.15 53.0 µM 2.47E-4 60 8.09E-4
14 SGTC_542 3381-0244 3.15 89.3 µM 2.54E-4 78 8.28E-4
15 SGTC_1006 4099-6547 3.14 255.0 µM 2.62E-4 57 8.48E-4
16 SGTC_1080 nisoldipine 3.10 24.4 µM 3.10E-4 TSC3-RPN4 61 9.77E-4
17 SGTC_1773 st048542 3.00 73.6 µM 4.66E-4 93 0.00137
18 SGTC_539 4859-0462 2.93 2.5 µM 6.13E-4 RNA pol III & RNase P/MRP 70 0.00172
19 SGTC_930 2810-3379 2.88 265.0 µM 7.34E-4 56 0.00199
20 SGTC_2482 5981709 2.84 142.7 µM 8.52E-4 PDR1 112 0.00225

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.126 3.03E-13 YOR291W YPK9 hom Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome
0.122 1.31E-12 YPR009W SUT2 hom Putative transcription factor; multicopy suppressor of mutations that cause low activity of the cAMP/protein kinase A pathway; highly similar to Sut1p
0.115 2.55E-11 YOR378W_p YOR378W_p hom Putative paralog of ATR1, but not required for boron tolerance; member of the DHA2 family of drug:H+ antiporters; YOR378W is not an essential gene
0.104 1.69E-9 YPR083W MDM36 hom Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission
0.103 2.48E-9 YBR080C SEC18 het ATPase required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, autophagy, and protein secretion; releases Sec17p from SNAP complexes; has similarity to mammalian NSF
0.101 4.26E-9 YGL110C CUE3 hom Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination
0.100 7.51E-9 YOL030W GAS5 hom 1,3-beta-glucanosyltransferase, has similarity to Gas1p; localizes to the cell wall
0.098 1.50E-8 YDL218W_p YDL218W_p hom Putative protein of unknown function; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions; expression also induced in cells treated with the mycotoxin patulin
0.094 4.21E-8 YOR288C MPD1 hom Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation
0.094 5.24E-8 YBR092C PHO3 hom Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin
0.090 1.74E-7 YBR153W RIB7 het Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway
0.088 3.16E-7 YPL263C KEL3 hom Cytoplasmic protein of unknown function
0.088 3.27E-7 YPL190C NAB3 het RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing
0.086 6.77E-7 YBL068W PRS4 hom 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication
0.084 1.24E-6 YBR296C-A_p YBR296C-A_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching