YKL040C / NFU1

Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL040C / NFU1

Click on Significant Values for Screen Details ID:SGTC_59|Compound:0330-0160|FD-Score:3.24|P-value:1.93E-4 ID:SGTC_227|Compound:fluconazole|FD-Score:3.27|P-value:1.74E-4 ID:SGTC_279|Compound:1273-0055|FD-Score:3.10|P-value:3.44E-4 ID:SGTC_318|Compound:1488-0629|FD-Score:4.10|P-value:3.48E-6 ID:SGTC_447|Compound:ml7|FD-Score:-3.04|P-value:4.55E-4 ID:SGTC_655|Compound:k060-0012|FD-Score:2.84|P-value:9.43E-4 ID:SGTC_668|Compound:1682-0499|FD-Score:3.74|P-value:2.13E-5 ID:SGTC_773|Compound:4358-1457|FD-Score:3.11|P-value:3.29E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:3.36|P-value:1.17E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:-4.16|P-value:2.84E-6 ID:SGTC_965|Compound:1291-5240|FD-Score:-4.57|P-value:3.03E-7 ID:SGTC_1422|Compound:4031-0024|FD-Score:2.99|P-value:5.27E-4 ID:SGTC_1963|Compound:st076565|FD-Score:3.33|P-value:1.30E-4 ID:SGTC_1972|Compound:st070201|FD-Score:-3.04|P-value:4.48E-4 ID:SGTC_2298|Compound:7976326|FD-Score:3.91|P-value:9.16E-6 ID:SGTC_2301|Compound:7769476|FD-Score:-4.40|P-value:7.59E-7 ID:SGTC_2386|Compound:9075165|FD-Score:-3.63|P-value:3.64E-5 ID:SGTC_2476|Compound:5806916|FD-Score:3.29|P-value:1.57E-4 ID:SGTC_2480|Compound:5767542|FD-Score:-4.96|P-value:3.11E-8 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:-5.66|P-value:3.19E-10 ID:SGTC_2764|Compound:methyldopa|FD-Score:3.53|P-value:5.45E-5 ID:SGTC_2774|Compound:tamoxifen|FD-Score:-3.38|P-value:1.11E-4 ID:SGTC_2782|Compound:7736369|FD-Score:3.46|P-value:7.53E-5 ID:SGTC_2796|Compound:7949634|FD-Score:3.24|P-value:1.94E-4 ID:SGTC_2799|Compound:7812892|FD-Score:3.34|P-value:1.27E-4 ID:SGTC_2801|Compound:7907800|FD-Score:3.75|P-value:1.96E-5 ID:SGTC_2929|Compound:9013348|FD-Score:3.47|P-value:7.15E-5 ID:SGTC_2933|Compound:9026794|FD-Score:3.42|P-value:8.90E-5 ID:SGTC_2977|Compound:9011197|FD-Score:-3.82|P-value:1.54E-5 ID:SGTC_2978|Compound:9011784|FD-Score:-4.93|P-value:3.62E-8 ID:SGTC_2987|Compound:9018338|FD-Score:3.02|P-value:4.61E-4 ID:SGTC_3137|Compound:9097352|FD-Score:3.72|P-value:2.30E-5 ID:SGTC_3351|Compound:9150312|FD-Score:-3.13|P-value:3.22E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:3.24|P-value:1.93E-4 ID:SGTC_227|Compound:fluconazole|FD-Score:3.27|P-value:1.74E-4 ID:SGTC_279|Compound:1273-0055|FD-Score:3.10|P-value:3.44E-4 ID:SGTC_318|Compound:1488-0629|FD-Score:4.10|P-value:3.48E-6 ID:SGTC_447|Compound:ml7|FD-Score:-3.04|P-value:4.55E-4 ID:SGTC_655|Compound:k060-0012|FD-Score:2.84|P-value:9.43E-4 ID:SGTC_668|Compound:1682-0499|FD-Score:3.74|P-value:2.13E-5 ID:SGTC_773|Compound:4358-1457|FD-Score:3.11|P-value:3.29E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:3.36|P-value:1.17E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:-4.16|P-value:2.84E-6 ID:SGTC_965|Compound:1291-5240|FD-Score:-4.57|P-value:3.03E-7 ID:SGTC_1422|Compound:4031-0024|FD-Score:2.99|P-value:5.27E-4 ID:SGTC_1963|Compound:st076565|FD-Score:3.33|P-value:1.30E-4 ID:SGTC_1972|Compound:st070201|FD-Score:-3.04|P-value:4.48E-4 ID:SGTC_2298|Compound:7976326|FD-Score:3.91|P-value:9.16E-6 ID:SGTC_2301|Compound:7769476|FD-Score:-4.40|P-value:7.59E-7 ID:SGTC_2386|Compound:9075165|FD-Score:-3.63|P-value:3.64E-5 ID:SGTC_2476|Compound:5806916|FD-Score:3.29|P-value:1.57E-4 ID:SGTC_2480|Compound:5767542|FD-Score:-4.96|P-value:3.11E-8 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:-5.66|P-value:3.19E-10 ID:SGTC_2764|Compound:methyldopa|FD-Score:3.53|P-value:5.45E-5 ID:SGTC_2774|Compound:tamoxifen|FD-Score:-3.38|P-value:1.11E-4 ID:SGTC_2782|Compound:7736369|FD-Score:3.46|P-value:7.53E-5 ID:SGTC_2796|Compound:7949634|FD-Score:3.24|P-value:1.94E-4 ID:SGTC_2799|Compound:7812892|FD-Score:3.34|P-value:1.27E-4 ID:SGTC_2801|Compound:7907800|FD-Score:3.75|P-value:1.96E-5 ID:SGTC_2929|Compound:9013348|FD-Score:3.47|P-value:7.15E-5 ID:SGTC_2933|Compound:9026794|FD-Score:3.42|P-value:8.90E-5 ID:SGTC_2977|Compound:9011197|FD-Score:-3.82|P-value:1.54E-5 ID:SGTC_2978|Compound:9011784|FD-Score:-4.93|P-value:3.62E-8 ID:SGTC_2987|Compound:9018338|FD-Score:3.02|P-value:4.61E-4 ID:SGTC_3137|Compound:9097352|FD-Score:3.72|P-value:2.30E-5 ID:SGTC_3351|Compound:9150312|FD-Score:-3.13|P-value:3.22E-4

Top fitness defect scores for YKL040C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_318 1488-0629 4.10 67.2 µM 3.48E-6 copper-dependent oxidative stress 20 2.02E-5
2 SGTC_2298 7976326 3.91 193.8 µM 9.16E-6 10 4.57E-5
3 SGTC_2801 7907800 3.75 81.8 µM 1.96E-5 34 8.70E-5
4 SGTC_668 1682-0499 3.74 62.6 µM 2.13E-5 23 9.30E-5
5 SGTC_3137 9097352 3.72 49.5 µM 2.30E-5 50 9.95E-5
6 SGTC_2764 methyldopa 3.53 94.7 µM 5.45E-5 27 2.06E-4
7 SGTC_2929 9013348 3.47 24.6 µM 7.15E-5 40 2.59E-4
8 SGTC_2782 7736369 3.46 71.4 µM 7.53E-5 62 2.70E-4
9 SGTC_2933 9026794 3.42 32.5 µM 8.90E-5 48 3.11E-4
10 SGTC_878 0828-0289 3.36 13.7 µM 1.17E-4 75 3.92E-4
11 SGTC_2799 7812892 3.34 81.8 µM 1.27E-4 superoxide 49 4.19E-4
12 SGTC_1963 st076565 3.33 50.3 µM 1.30E-4 30 4.29E-4
13 SGTC_2476 5806916 3.29 10.5 µM 1.57E-4 32 5.01E-4
14 SGTC_227 fluconazole 3.27 33.5 µM 1.74E-4 azole & statin 122 5.47E-4
15 SGTC_59 0330-0160 3.24 1.3 µM 1.93E-4 175 5.96E-4
16 SGTC_2796 7949634 3.24 45.5 µM 1.94E-4 49 5.99E-4
17 SGTC_773 4358-1457 3.11 44.6 µM 3.29E-4 75 9.37E-4
18 SGTC_279 1273-0055 3.10 2.9 µM 3.44E-4 azole & statin 71 9.75E-4
19 SGTC_2987 9018338 3.02 71.4 µM 4.61E-4 RPP1 & pyrimidine depletion 71 0.00125
20 SGTC_1422 4031-0024 2.99 15.5 µM 5.27E-4 iron homeostasis 50 0.00140

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.132 2.04E-14 YGL091C NBP35 het Essential iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Cfd1p that is involved in iron-sulfur protein assembly in the cytosol; similar to P-loop NTPases
0.112 8.07E-11 YDL201W TRM8 hom Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
0.109 2.49E-10 YFL028C CAF16 hom Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator
0.101 4.07E-9 YOL149W DCP1 het Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress
0.099 8.56E-9 YOR028C CIN5 hom Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions; CIN5 has a paralog, YAP6, that arose from the whole genome duplication
0.096 2.56E-8 YOR174W MED4 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.094 4.72E-8 YGL140C_p YGL140C_p hom Putative protein of unknown function; non-essential gene; contains multiple predicted transmembrane domains
0.093 7.08E-8 YGR173W RBG2 hom Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein
0.091 1.46E-7 YBR248C HIS7 hom Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor
0.089 2.21E-7 YOL077C BRX1 het Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif
0.088 3.27E-7 YHL015W-A_p YHL015W-A_p hom Putative protein of unknown function
0.088 3.28E-7 YMR156C TPP1 hom DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase
0.085 8.64E-7 YDR055W PST1 hom Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1
0.085 8.95E-7 YAL043C PTA1 het Subunit of holo-CPF, a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII
0.084 1.07E-6 YKL027W YKL027W hom Protein of unknown function, localized to the mitochondrial outer membrane