YKL071W_p

Putative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL071W / YKL071W

Click on Significant Values for Screen Details ID:SGTC_113|Compound:0099-0221|FD-Score:3.48|P-value:3.33E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:-3.62|P-value:1.91E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:4.03|P-value:3.96E-5 ID:SGTC_622|Compound:1348-1566|FD-Score:3.23|P-value:7.76E-4 ID:SGTC_628|Compound:1636-0253|FD-Score:-3.31|P-value:5.95E-4 ID:SGTC_637|Compound:1181-0519|FD-Score:5.22|P-value:1.58E-7 ID:SGTC_644|Compound:1498-1197|FD-Score:4.44|P-value:6.93E-6 ID:SGTC_645|Compound:0845-0943|FD-Score:-4.22|P-value:1.74E-5 ID:SGTC_932|Compound:2857-0803|FD-Score:4.03|P-value:4.01E-5 ID:SGTC_937|Compound:3122-0005|FD-Score:6.27|P-value:4.00E-10 ID:SGTC_1033|Compound:k072-0102|FD-Score:3.94|P-value:5.60E-5 ID:SGTC_1146|Compound:3579-1023|FD-Score:-3.18|P-value:9.03E-4 ID:SGTC_1252|Compound:0493-0875|FD-Score:-3.26|P-value:7.04E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:3.29|P-value:6.41E-4 ID:SGTC_1910|Compound:st062352|FD-Score:4.23|P-value:1.69E-5 ID:SGTC_1916|Compound:st067606|FD-Score:-3.17|P-value:9.41E-4 ID:SGTC_1958|Compound:st076513|FD-Score:5.09|P-value:3.09E-7 ID:SGTC_2099|Compound:5377090|FD-Score:3.87|P-value:7.56E-5 ID:SGTC_2160|Compound:5660466|FD-Score:4.21|P-value:1.85E-5 ID:SGTC_2231|Compound:micatex|FD-Score:3.35|P-value:5.21E-4 ID:SGTC_2280|Compound:7785178|FD-Score:-5.69|P-value:1.24E-8 ID:SGTC_2284|Compound:7945075|FD-Score:-3.75|P-value:1.17E-4 ID:SGTC_2285|Compound:7945142|FD-Score:9.89|P-value:1.66E-22 ID:SGTC_2345|Compound:9052315|FD-Score:-3.56|P-value:2.44E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:3.89|P-value:6.94E-5 ID:SGTC_2414|Compound:st077232|FD-Score:-3.52|P-value:2.79E-4 ID:SGTC_2433|Compound:5607874|FD-Score:-3.16|P-value:9.72E-4 ID:SGTC_2662|Compound:daunorubicin|FD-Score:-3.43|P-value:3.91E-4 ID:SGTC_2671|Compound:danthron|FD-Score:-3.25|P-value:7.18E-4 ID:SGTC_2683|Compound:piperine|FD-Score:4.41|P-value:7.97E-6 ID:SGTC_2984|Compound:9022109|FD-Score:3.20|P-value:8.75E-4 ID:SGTC_2987|Compound:9018338|FD-Score:3.47|P-value:3.38E-4 ID:SGTC_3310|Compound:9130229|FD-Score:3.51|P-value:2.89E-4 ID:SGTC_3338|Compound:9144300|FD-Score:3.52|P-value:2.87E-4 ID:SGTC_113|Compound:0099-0221|FD-Score:3.48|P-value:3.33E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:-3.62|P-value:1.91E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:4.03|P-value:3.96E-5 ID:SGTC_622|Compound:1348-1566|FD-Score:3.23|P-value:7.76E-4 ID:SGTC_628|Compound:1636-0253|FD-Score:-3.31|P-value:5.95E-4 ID:SGTC_637|Compound:1181-0519|FD-Score:5.22|P-value:1.58E-7 ID:SGTC_644|Compound:1498-1197|FD-Score:4.44|P-value:6.93E-6 ID:SGTC_645|Compound:0845-0943|FD-Score:-4.22|P-value:1.74E-5 ID:SGTC_932|Compound:2857-0803|FD-Score:4.03|P-value:4.01E-5 ID:SGTC_937|Compound:3122-0005|FD-Score:6.27|P-value:4.00E-10 ID:SGTC_1033|Compound:k072-0102|FD-Score:3.94|P-value:5.60E-5 ID:SGTC_1146|Compound:3579-1023|FD-Score:-3.18|P-value:9.03E-4 ID:SGTC_1252|Compound:0493-0875|FD-Score:-3.26|P-value:7.04E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:3.29|P-value:6.41E-4 ID:SGTC_1910|Compound:st062352|FD-Score:4.23|P-value:1.69E-5 ID:SGTC_1916|Compound:st067606|FD-Score:-3.17|P-value:9.41E-4 ID:SGTC_1958|Compound:st076513|FD-Score:5.09|P-value:3.09E-7 ID:SGTC_2099|Compound:5377090|FD-Score:3.87|P-value:7.56E-5 ID:SGTC_2160|Compound:5660466|FD-Score:4.21|P-value:1.85E-5 ID:SGTC_2231|Compound:micatex|FD-Score:3.35|P-value:5.21E-4 ID:SGTC_2280|Compound:7785178|FD-Score:-5.69|P-value:1.24E-8 ID:SGTC_2284|Compound:7945075|FD-Score:-3.75|P-value:1.17E-4 ID:SGTC_2285|Compound:7945142|FD-Score:9.89|P-value:1.66E-22 ID:SGTC_2345|Compound:9052315|FD-Score:-3.56|P-value:2.44E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:3.89|P-value:6.94E-5 ID:SGTC_2414|Compound:st077232|FD-Score:-3.52|P-value:2.79E-4 ID:SGTC_2433|Compound:5607874|FD-Score:-3.16|P-value:9.72E-4 ID:SGTC_2662|Compound:daunorubicin|FD-Score:-3.43|P-value:3.91E-4 ID:SGTC_2671|Compound:danthron|FD-Score:-3.25|P-value:7.18E-4 ID:SGTC_2683|Compound:piperine|FD-Score:4.41|P-value:7.97E-6 ID:SGTC_2984|Compound:9022109|FD-Score:3.20|P-value:8.75E-4 ID:SGTC_2987|Compound:9018338|FD-Score:3.47|P-value:3.38E-4 ID:SGTC_3310|Compound:9130229|FD-Score:3.51|P-value:2.89E-4 ID:SGTC_3338|Compound:9144300|FD-Score:3.52|P-value:2.87E-4

Top fitness defect scores for YKL071W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_2285 7945142 9.89 200.0 µM 1.66E-22 1 2.31E-23
2 SGTC_937 3122-0005 6.27 46.7 µM 4.00E-10 5 1.77E-10
3 SGTC_637 1181-0519 5.22 7.3 µM 1.58E-7 fatty acid desaturase (OLE1) 60 8.96E-8
4 SGTC_1958 st076513 5.09 41.9 µM 3.09E-7 60S ribosome export 13 1.80E-7
5 SGTC_644 1498-1197 4.44 63.3 µM 6.93E-6 6 4.56E-6
6 SGTC_2683 piperine 4.41 63.0 µM 7.97E-6 10 5.27E-6
7 SGTC_1910 st062352 4.23 60.4 µM 1.69E-5 24 1.15E-5
8 SGTC_2160 5660466 4.21 132.9 µM 1.85E-5 60S ribosome export 10 1.27E-5
9 SGTC_268 camptothecin 4.03 39.6 µM 3.96E-5 DNA damage response 66 2.79E-5
10 SGTC_932 2857-0803 4.03 154.0 µM 4.01E-5 25 2.83E-5
11 SGTC_1033 k072-0102 3.94 21.3 µM 5.60E-5 60S ribosome export 23 4.01E-5
12 SGTC_2406 ethylparaben 3.89 405.7 µM 6.94E-5 43 5.01E-5
13 SGTC_2099 5377090 3.87 177.4 µM 7.56E-5 25 5.47E-5
14 SGTC_3338 9144300 3.52 39.6 µM 2.87E-4 Golgi 38 2.19E-4
15 SGTC_3310 9130229 3.51 44.1 µM 2.89E-4 Golgi 42 2.20E-4
16 SGTC_113 0099-0221 3.48 2.5 µM 3.33E-4 45 2.55E-4
17 SGTC_2987 9018338 3.47 71.4 µM 3.38E-4 RPP1 & pyrimidine depletion 37 2.59E-4
18 SGTC_2231 micatex 3.35 6.8 µM 5.21E-4 29 4.07E-4
19 SGTC_1255 0566-0045 3.29 221.0 µM 6.41E-4 excess fatty acid 53 5.04E-4
20 SGTC_622 1348-1566 3.23 26.3 µM 7.76E-4 45 6.16E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.222 1.14E-38 YDR013W PSF1 het Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
0.116 1.83E-11 YOR135C_d IRC14_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci
0.114 2.98E-11 YLR209C PNP1 hom Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway
0.100 6.15E-9 YMR251W-A HOR7 hom Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication
0.098 1.16E-8 YKR061W KTR2 hom Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication
0.094 5.66E-8 YNL281W HCH1 hom Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress
0.093 6.52E-8 YBR210W ERV15 hom Protein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication
0.093 7.19E-8 YGR144W THI4 hom Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents
0.089 2.40E-7 YIL078W THS1 het Threonyl-tRNA synthetase, essential cytoplasmic protein
0.087 4.29E-7 YMR086W SEG1 hom Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes
0.086 5.95E-7 YLR461W PAU4 hom Member of the seripauperin multigene family encoded mainly in subtelomeric regions; active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme
0.085 8.43E-7 YGR138C TPO2 hom Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; transcription of TPO2 is regulated by Haa1p; TPO2 has a paralog, TPO3, that arose from the whole genome duplication
0.085 8.62E-7 YBR065C ECM2 hom Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p
0.084 1.23E-6 YNL272C SEC2 het Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles
0.082 2.14E-6 YDR246W-A_p YDR246W-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR