YKL074C / MUD2

Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL074C / MUD2

Click on Significant Values for Screen Details ID:SGTC_46|Compound:0329-0187a|FD-Score:4.63|P-value:5.98E-7 ID:SGTC_58|Compound:0957-0393|FD-Score:-6.97|P-value:5.75E-13 ID:SGTC_69|Compound:0096-0274|FD-Score:3.50|P-value:1.09E-4 ID:SGTC_77|Compound:0828-0286|FD-Score:-4.01|P-value:2.54E-5 ID:SGTC_223|Compound:busulfan|FD-Score:2.98|P-value:7.84E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.20|P-value:6.50E-4 ID:SGTC_430|Compound:0958-0115|FD-Score:-5.61|P-value:5.92E-9 ID:SGTC_560|Compound:2332-1943|FD-Score:-3.17|P-value:7.10E-4 ID:SGTC_567|Compound:1080-0542|FD-Score:2.94|P-value:9.30E-4 ID:SGTC_653|Compound:0203-0016|FD-Score:3.76|P-value:3.78E-5 ID:SGTC_779|Compound:1360-0071|FD-Score:3.20|P-value:3.56E-4 ID:SGTC_783|Compound:0848-0141|FD-Score:-3.86|P-value:4.79E-5 ID:SGTC_1216|Compound:1165-0504|FD-Score:2.94|P-value:9.08E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:3.76|P-value:3.75E-5 ID:SGTC_1247|Compound:0448-0043|FD-Score:3.01|P-value:7.24E-4 ID:SGTC_1356|Compound:1502-0781|FD-Score:-3.93|P-value:3.52E-5 ID:SGTC_1430|Compound:4049-0248|FD-Score:3.00|P-value:7.50E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:2.94|P-value:9.11E-4 ID:SGTC_1521|Compound:st012942|FD-Score:3.97|P-value:1.45E-5 ID:SGTC_1522|Compound:st012842|FD-Score:-3.33|P-value:4.08E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:-3.09|P-value:9.37E-4 ID:SGTC_1713|Compound:st033146|FD-Score:5.82|P-value:5.66E-10 ID:SGTC_1722|Compound:st034304|FD-Score:-5.79|P-value:1.95E-9 ID:SGTC_1754|Compound:st042065|FD-Score:-3.52|P-value:1.91E-4 ID:SGTC_2016|Compound:5121758|FD-Score:-4.24|P-value:8.80E-6 ID:SGTC_2023|Compound:5144124|FD-Score:-3.28|P-value:4.85E-4 ID:SGTC_2103|Compound:5378138|FD-Score:3.10|P-value:5.12E-4 ID:SGTC_2123|Compound:5259487|FD-Score:3.13|P-value:4.69E-4 ID:SGTC_2124|Compound:5246320|FD-Score:3.39|P-value:1.74E-4 ID:SGTC_2134|Compound:5324836|FD-Score:-3.22|P-value:5.94E-4 ID:SGTC_2292|Compound:7671323|FD-Score:3.48|P-value:1.19E-4 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:3.61|P-value:7.09E-5 ID:SGTC_2469|Compound:5655019|FD-Score:3.22|P-value:3.29E-4 ID:SGTC_2471|Compound:5466956|FD-Score:-3.27|P-value:5.05E-4 ID:SGTC_2845|Compound:9003024|FD-Score:3.77|P-value:3.48E-5 ID:SGTC_2896|Compound:9052656|FD-Score:3.70|P-value:4.81E-5 ID:SGTC_2920|Compound:7982904|FD-Score:4.91|P-value:1.33E-7 ID:SGTC_2921|Compound:7974240|FD-Score:3.55|P-value:9.17E-5 ID:SGTC_2970|Compound:9090938|FD-Score:3.16|P-value:4.09E-4 ID:SGTC_3037|Compound:9091026|FD-Score:-3.92|P-value:3.74E-5 ID:SGTC_46|Compound:0329-0187a|FD-Score:4.63|P-value:5.98E-7 ID:SGTC_58|Compound:0957-0393|FD-Score:-6.97|P-value:5.75E-13 ID:SGTC_69|Compound:0096-0274|FD-Score:3.50|P-value:1.09E-4 ID:SGTC_77|Compound:0828-0286|FD-Score:-4.01|P-value:2.54E-5 ID:SGTC_223|Compound:busulfan|FD-Score:2.98|P-value:7.84E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.20|P-value:6.50E-4 ID:SGTC_430|Compound:0958-0115|FD-Score:-5.61|P-value:5.92E-9 ID:SGTC_560|Compound:2332-1943|FD-Score:-3.17|P-value:7.10E-4 ID:SGTC_567|Compound:1080-0542|FD-Score:2.94|P-value:9.30E-4 ID:SGTC_653|Compound:0203-0016|FD-Score:3.76|P-value:3.78E-5 ID:SGTC_779|Compound:1360-0071|FD-Score:3.20|P-value:3.56E-4 ID:SGTC_783|Compound:0848-0141|FD-Score:-3.86|P-value:4.79E-5 ID:SGTC_1216|Compound:1165-0504|FD-Score:2.94|P-value:9.08E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:3.76|P-value:3.75E-5 ID:SGTC_1247|Compound:0448-0043|FD-Score:3.01|P-value:7.24E-4 ID:SGTC_1356|Compound:1502-0781|FD-Score:-3.93|P-value:3.52E-5 ID:SGTC_1430|Compound:4049-0248|FD-Score:3.00|P-value:7.50E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:2.94|P-value:9.11E-4 ID:SGTC_1521|Compound:st012942|FD-Score:3.97|P-value:1.45E-5 ID:SGTC_1522|Compound:st012842|FD-Score:-3.33|P-value:4.08E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:-3.09|P-value:9.37E-4 ID:SGTC_1713|Compound:st033146|FD-Score:5.82|P-value:5.66E-10 ID:SGTC_1722|Compound:st034304|FD-Score:-5.79|P-value:1.95E-9 ID:SGTC_1754|Compound:st042065|FD-Score:-3.52|P-value:1.91E-4 ID:SGTC_2016|Compound:5121758|FD-Score:-4.24|P-value:8.80E-6 ID:SGTC_2023|Compound:5144124|FD-Score:-3.28|P-value:4.85E-4 ID:SGTC_2103|Compound:5378138|FD-Score:3.10|P-value:5.12E-4 ID:SGTC_2123|Compound:5259487|FD-Score:3.13|P-value:4.69E-4 ID:SGTC_2124|Compound:5246320|FD-Score:3.39|P-value:1.74E-4 ID:SGTC_2134|Compound:5324836|FD-Score:-3.22|P-value:5.94E-4 ID:SGTC_2292|Compound:7671323|FD-Score:3.48|P-value:1.19E-4 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:3.61|P-value:7.09E-5 ID:SGTC_2469|Compound:5655019|FD-Score:3.22|P-value:3.29E-4 ID:SGTC_2471|Compound:5466956|FD-Score:-3.27|P-value:5.05E-4 ID:SGTC_2845|Compound:9003024|FD-Score:3.77|P-value:3.48E-5 ID:SGTC_2896|Compound:9052656|FD-Score:3.70|P-value:4.81E-5 ID:SGTC_2920|Compound:7982904|FD-Score:4.91|P-value:1.33E-7 ID:SGTC_2921|Compound:7974240|FD-Score:3.55|P-value:9.17E-5 ID:SGTC_2970|Compound:9090938|FD-Score:3.16|P-value:4.09E-4 ID:SGTC_3037|Compound:9091026|FD-Score:-3.92|P-value:3.74E-5

Top fitness defect scores for YKL074C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1713 st033146 5.82 12.8 µM 5.66E-10 5 2.91E-9
2 SGTC_2920 7982904 4.91 15.8 µM 1.33E-7 5 4.62E-7
3 SGTC_46 0329-0187a 4.63 2.2 µM 5.98E-7 49 1.86E-6
4 SGTC_1521 st012942 3.97 49.4 µM 1.45E-5 31 3.55E-5
5 SGTC_2845 9003024 3.77 43.0 µM 3.48E-5 amide catabolism 51 8.01E-5
6 SGTC_1230 0170-0365 3.76 3.8 µM 3.75E-5 endomembrane recycling 79 8.59E-5
7 SGTC_653 0203-0016 3.76 55.2 µM 3.78E-5 19 8.64E-5
8 SGTC_2896 9052656 3.70 71.4 µM 4.81E-5 endomembrane recycling 138 1.08E-4
9 SGTC_2454 5-benzylidenerhodanine 3.61 10.4 µM 7.09E-5 TRP & mitochondrial translation 51 1.55E-4
10 SGTC_2921 7974240 3.55 10.7 µM 9.17E-5 70 1.96E-4
11 SGTC_69 0096-0274 3.50 206.7 µM 1.09E-4 mitochondrial processes 66 2.31E-4
12 SGTC_2292 7671323 3.48 168.1 µM 1.19E-4 RNA processing & uracil transport 54 2.50E-4
13 SGTC_2124 5246320 3.39 200.0 µM 1.74E-4 heme biosynthesis & mitochondrial translocase 38 3.55E-4
14 SGTC_2469 5655019 3.22 90.8 µM 3.29E-4 43 6.38E-4
15 SGTC_779 1360-0071 3.20 233.0 µM 3.56E-4 fatty acid desaturase (OLE1) 67 6.86E-4
16 SGTC_2970 9090938 3.16 41.9 µM 4.09E-4 RNA processing & uracil transport 90 7.80E-4
17 SGTC_2123 5259487 3.13 200.0 µM 4.69E-4 46 8.85E-4
18 SGTC_2103 5378138 3.10 10.0 µM 5.12E-4 54 9.61E-4
19 SGTC_1247 0448-0043 3.01 5.8 µM 7.24E-4 ERG2 110 0.00132
20 SGTC_1430 4049-0248 3.00 69.1 µM 7.50E-4 64 0.00137

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.166 4.00E-22 YNL219C ALG9 hom Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation
0.141 2.03E-16 YNL067W RPL9B hom Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6
0.127 1.64E-13 YGR092W DBF2 hom Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis
0.124 6.75E-13 YNL157W IGO1 hom Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo2p
0.122 1.39E-12 YGR124W ASN2 hom Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication
0.120 2.92E-12 YDR156W RPA14 hom RNA polymerase I subunit A14
0.116 1.82E-11 YGR153W_p YGR153W_p hom Putative protein of unknown function
0.109 2.48E-10 YDL180W_p YDL180W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
0.109 2.72E-10 YDR500C RPL37B hom Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication
0.107 5.97E-10 YDR067C_p OCA6_p hom Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT
0.104 1.34E-9 YOR274W MOD5 hom Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis
0.104 1.57E-9 YDR147W EKI1 hom Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication
0.103 2.20E-9 YGL038C OCH1 hom Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins
0.103 2.24E-9 YKR015C_p YKR015C_p hom Putative protein of unknown function
0.102 3.28E-9 YPL208W RKM1 hom SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp)