YKL092C / BUD2

GTPase activating factor for Rsr1p/Bud1p; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; mutant deficient in mitotic spindle position checkpoint

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL092C / BUD2

Click on Significant Values for Screen Details ID:SGTC_99|Compound:0109-0120|FD-Score:3.29|P-value:3.16E-4 ID:SGTC_322|Compound:k048-0136|FD-Score:-3.66|P-value:6.87E-5 ID:SGTC_439|Compound:amiodarone|FD-Score:3.23|P-value:3.94E-4 ID:SGTC_500|Compound:h-89|FD-Score:3.11|P-value:6.27E-4 ID:SGTC_776|Compound:4476-4378|FD-Score:-4.30|P-value:3.83E-6 ID:SGTC_783|Compound:0848-0141|FD-Score:-3.38|P-value:2.15E-4 ID:SGTC_839|Compound:0122-0028|FD-Score:3.12|P-value:6.06E-4 ID:SGTC_857|Compound:0438-0306|FD-Score:3.51|P-value:1.36E-4 ID:SGTC_1050|Compound:3232-1156|FD-Score:3.49|P-value:1.44E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.40|P-value:2.00E-4 ID:SGTC_1417|Compound:3966-0327|FD-Score:3.12|P-value:5.92E-4 ID:SGTC_1539|Compound:benzyladenosine|FD-Score:-4.44|P-value:1.88E-6 ID:SGTC_1750|Compound:st081859|FD-Score:3.44|P-value:1.78E-4 ID:SGTC_1752|Compound:st044819|FD-Score:-3.42|P-value:1.82E-4 ID:SGTC_1756|Compound:st041558|FD-Score:3.42|P-value:1.92E-4 ID:SGTC_1765|Compound:st044815|FD-Score:-4.00|P-value:1.55E-5 ID:SGTC_1767|Compound:st044992|FD-Score:3.13|P-value:5.84E-4 ID:SGTC_1809|Compound:st050081|FD-Score:3.38|P-value:2.21E-4 ID:SGTC_2175|Compound:5807670|FD-Score:3.26|P-value:3.49E-4 ID:SGTC_2220|Compound:6782180|FD-Score:3.11|P-value:6.22E-4 ID:SGTC_2365|Compound:9042475|FD-Score:-3.35|P-value:2.44E-4 ID:SGTC_2411|Compound:st077133|FD-Score:3.36|P-value:2.47E-4 ID:SGTC_2439|Compound:5492030|FD-Score:3.44|P-value:1.80E-4 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:5.68|P-value:1.84E-9 ID:SGTC_2563|Compound:tomatine|FD-Score:3.23|P-value:4.02E-4 ID:SGTC_2807|Compound:7945238|FD-Score:3.27|P-value:3.43E-4 ID:SGTC_2810|Compound:7959871|FD-Score:3.38|P-value:2.27E-4 ID:SGTC_2814|Compound:7977482|FD-Score:4.39|P-value:2.60E-6 ID:SGTC_2858|Compound:9025781|FD-Score:2.99|P-value:9.58E-4 ID:SGTC_3081|Compound:9117243|FD-Score:3.25|P-value:3.68E-4 ID:SGTC_3157|Compound:9099274|FD-Score:3.34|P-value:2.58E-4 ID:SGTC_3294|Compound:9122953|FD-Score:-3.50|P-value:1.33E-4 ID:SGTC_3344|Compound:9148862|FD-Score:-5.98|P-value:2.34E-10 ID:SGTC_99|Compound:0109-0120|FD-Score:3.29|P-value:3.16E-4 ID:SGTC_322|Compound:k048-0136|FD-Score:-3.66|P-value:6.87E-5 ID:SGTC_439|Compound:amiodarone|FD-Score:3.23|P-value:3.94E-4 ID:SGTC_500|Compound:h-89|FD-Score:3.11|P-value:6.27E-4 ID:SGTC_776|Compound:4476-4378|FD-Score:-4.30|P-value:3.83E-6 ID:SGTC_783|Compound:0848-0141|FD-Score:-3.38|P-value:2.15E-4 ID:SGTC_839|Compound:0122-0028|FD-Score:3.12|P-value:6.06E-4 ID:SGTC_857|Compound:0438-0306|FD-Score:3.51|P-value:1.36E-4 ID:SGTC_1050|Compound:3232-1156|FD-Score:3.49|P-value:1.44E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.40|P-value:2.00E-4 ID:SGTC_1417|Compound:3966-0327|FD-Score:3.12|P-value:5.92E-4 ID:SGTC_1539|Compound:benzyladenosine|FD-Score:-4.44|P-value:1.88E-6 ID:SGTC_1750|Compound:st081859|FD-Score:3.44|P-value:1.78E-4 ID:SGTC_1752|Compound:st044819|FD-Score:-3.42|P-value:1.82E-4 ID:SGTC_1756|Compound:st041558|FD-Score:3.42|P-value:1.92E-4 ID:SGTC_1765|Compound:st044815|FD-Score:-4.00|P-value:1.55E-5 ID:SGTC_1767|Compound:st044992|FD-Score:3.13|P-value:5.84E-4 ID:SGTC_1809|Compound:st050081|FD-Score:3.38|P-value:2.21E-4 ID:SGTC_2175|Compound:5807670|FD-Score:3.26|P-value:3.49E-4 ID:SGTC_2220|Compound:6782180|FD-Score:3.11|P-value:6.22E-4 ID:SGTC_2365|Compound:9042475|FD-Score:-3.35|P-value:2.44E-4 ID:SGTC_2411|Compound:st077133|FD-Score:3.36|P-value:2.47E-4 ID:SGTC_2439|Compound:5492030|FD-Score:3.44|P-value:1.80E-4 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:5.68|P-value:1.84E-9 ID:SGTC_2563|Compound:tomatine|FD-Score:3.23|P-value:4.02E-4 ID:SGTC_2807|Compound:7945238|FD-Score:3.27|P-value:3.43E-4 ID:SGTC_2810|Compound:7959871|FD-Score:3.38|P-value:2.27E-4 ID:SGTC_2814|Compound:7977482|FD-Score:4.39|P-value:2.60E-6 ID:SGTC_2858|Compound:9025781|FD-Score:2.99|P-value:9.58E-4 ID:SGTC_3081|Compound:9117243|FD-Score:3.25|P-value:3.68E-4 ID:SGTC_3157|Compound:9099274|FD-Score:3.34|P-value:2.58E-4 ID:SGTC_3294|Compound:9122953|FD-Score:-3.50|P-value:1.33E-4 ID:SGTC_3344|Compound:9148862|FD-Score:-5.98|P-value:2.34E-10

Top fitness defect scores for YKL092C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2527 2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone 5.68 63.6 µM 1.84E-9 18 6.89E-9
2 SGTC_2814 7977482 4.39 43.0 µM 2.60E-6 38 5.76E-6
3 SGTC_857 0438-0306 3.51 1.4 µM 1.36E-4 copper-dependent oxidative stress 21 2.27E-4
4 SGTC_1050 3232-1156 3.49 69.3 µM 1.44E-4 RSC complex & mRNA processing 36 2.41E-4
5 SGTC_1750 st081859 3.44 2.4 µM 1.78E-4 endomembrane recycling 223 2.92E-4
6 SGTC_2439 5492030 3.44 7.2 µM 1.80E-4 23 2.95E-4
7 SGTC_1756 st041558 3.42 35.5 µM 1.92E-4 18 3.13E-4
8 SGTC_1809 st050081 3.38 57.6 µM 2.21E-4 31 3.57E-4
9 SGTC_2810 7959871 3.38 45.5 µM 2.27E-4 copper-dependent oxidative stress 63 3.67E-4
10 SGTC_2411 st077133 3.36 39.9 µM 2.47E-4 RNA pol III & RNase P/MRP 61 3.96E-4
11 SGTC_3157 9099274 3.34 49.5 µM 2.58E-4 35 4.14E-4
12 SGTC_99 0109-0120 3.29 622.8 µM 3.16E-4 32 4.99E-4
13 SGTC_2807 7945238 3.27 52.0 µM 3.43E-4 60 5.38E-4
14 SGTC_2175 5807670 3.26 165.3 µM 3.49E-4 43 5.48E-4
15 SGTC_3081 9117243 3.25 49.5 µM 3.68E-4 fatty acid desaturase (OLE1) 46 5.74E-4
16 SGTC_439 amiodarone 3.23 34.4 µM 3.94E-4 NEO1-PIK1 131 6.12E-4
17 SGTC_2563 tomatine 3.23 8.5 µM 4.02E-4 88 6.23E-4
18 SGTC_1767 st044992 3.13 70.3 µM 5.84E-4 53 8.83E-4
19 SGTC_1417 3966-0327 3.12 90.4 µM 5.92E-4 48 8.95E-4
20 SGTC_839 0122-0028 3.12 230.0 µM 6.06E-4 RSC complex & mRNA processing 77 9.14E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.193 1.20E-29 YLR097C HRT3 hom Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies
0.131 2.59E-14 YMR126C_p DLT1_p hom Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.121 1.74E-12 YLR323C CWC24 het Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p
0.121 1.75E-12 YHR003C YHR003C hom Protein of unknown function, localized to the mitochondrial outer membrane
0.115 2.63E-11 YMR171C EAR1 hom Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p
0.109 2.14E-10 YML072C TCB3 hom Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.109 2.36E-10 YGR153W_p YGR153W_p hom Putative protein of unknown function
0.108 3.33E-10 YPR029C APL4 hom Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport
0.108 4.17E-10 YPR153W YPR153W hom Putative protein of unknown function
0.105 1.05E-9 YDR110W FOB1 hom Nucleolar protein that binds the rDNA replication fork barrier (RFB) site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to RFB and rDNA repeat segregation; related to retroviral integrases
0.105 1.10E-9 YBR214W SDS24 hom Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis; protein abundance increases in response to DNA replication stress; SDS24 has a paralog, SDS23, that arose from the whole genome duplication
0.105 1.16E-9 YFL063W_d YFL063W_d hom Dubious open reading frame, based on available experimental and comparative sequence data
0.102 3.79E-9 YCR020C PET18 hom Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin
0.101 4.05E-9 YPL094C SEC62 het Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
0.101 4.52E-9 YMR164C MSS11 hom Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals