YKL103C / APE1

Vacuolar aminopeptidase yscI; zinc metalloproteinase that belongs to the peptidase family M18; often used as a marker protein in studies of autophagy and cytosol to vacuole targeting (CVT) pathway; protein increases in abundance and relative distribution to cytoplasmic foci increases upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL103C / APE1

Click on Significant Values for Screen Details ID:SGTC_69|Compound:0096-0274|FD-Score:4.00|P-value:3.24E-6 ID:SGTC_78|Compound:0336-0123|FD-Score:-2.85|P-value:7.05E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:2.75|P-value:9.60E-4 ID:SGTC_282|Compound:k297-0036|FD-Score:2.88|P-value:5.78E-4 ID:SGTC_286|Compound:k060-0067|FD-Score:-2.81|P-value:8.15E-4 ID:SGTC_316|Compound:k060-0003|FD-Score:3.43|P-value:5.52E-5 ID:SGTC_341|Compound:1486-1430|FD-Score:-4.80|P-value:3.63E-8 ID:SGTC_343|Compound:4130-2827|FD-Score:-3.16|P-value:2.01E-4 ID:SGTC_347|Compound:4534-1311|FD-Score:-3.80|P-value:1.04E-5 ID:SGTC_349|Compound:k015-0014|FD-Score:-3.24|P-value:1.43E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:-4.40|P-value:3.98E-7 ID:SGTC_358|Compound:0075-0058|FD-Score:4.61|P-value:9.90E-8 ID:SGTC_407|Compound:eburnamonine|FD-Score:-3.31|P-value:1.02E-4 ID:SGTC_531|Compound:3788-1495|FD-Score:3.43|P-value:5.46E-5 ID:SGTC_561|Compound:1550-0330|FD-Score:3.25|P-value:1.22E-4 ID:SGTC_867|Compound:0518-0118|FD-Score:-4.27|P-value:8.43E-7 ID:SGTC_1020|Compound:4335-0961|FD-Score:-3.33|P-value:9.41E-5 ID:SGTC_1021|Compound:4476-0760|FD-Score:2.75|P-value:9.59E-4 ID:SGTC_1022|Compound:4477-0945|FD-Score:-2.85|P-value:7.15E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:3.01|P-value:3.37E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:3.16|P-value:1.83E-4 ID:SGTC_1497|Compound:4476-2978|FD-Score:-3.48|P-value:4.82E-5 ID:SGTC_1719|Compound:st032227|FD-Score:3.25|P-value:1.24E-4 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:3.13|P-value:2.09E-4 ID:SGTC_2025|Compound:5147754|FD-Score:3.73|P-value:1.27E-5 ID:SGTC_2102|Compound:5377816|FD-Score:-3.07|P-value:2.91E-4 ID:SGTC_2125|Compound:5246583|FD-Score:6.05|P-value:4.80E-12 ID:SGTC_2233|Compound:sulbentine|FD-Score:-2.96|P-value:4.60E-4 ID:SGTC_2274|Compound:7947094|FD-Score:-2.79|P-value:9.07E-4 ID:SGTC_2321|Compound:7390090|FD-Score:2.82|P-value:7.25E-4 ID:SGTC_2340|Compound:7987427|FD-Score:4.03|P-value:2.68E-6 ID:SGTC_2348|Compound:9022543|FD-Score:4.82|P-value:2.81E-8 ID:SGTC_2437|Compound:5741531|FD-Score:3.17|P-value:1.73E-4 ID:SGTC_2463|Compound:5473429|FD-Score:3.61|P-value:2.32E-5 ID:SGTC_2477|Compound:5967686|FD-Score:-3.13|P-value:2.27E-4 ID:SGTC_2478|Compound:5763493|FD-Score:4.16|P-value:1.34E-6 ID:SGTC_2489|Compound:cantharidin|FD-Score:-3.09|P-value:2.67E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:2.85|P-value:6.53E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-3.17|P-value:1.90E-4 ID:SGTC_2714|Compound:st079313|FD-Score:3.68|P-value:1.62E-5 ID:SGTC_2835|Compound:9006588|FD-Score:3.52|P-value:3.50E-5 ID:SGTC_2836|Compound:9010338|FD-Score:-2.90|P-value:5.78E-4 ID:SGTC_2840|Compound:9008771|FD-Score:-2.76|P-value:9.96E-4 ID:SGTC_2842|Compound:9000675|FD-Score:4.20|P-value:1.07E-6 ID:SGTC_2882|Compound:9058044|FD-Score:-2.99|P-value:4.10E-4 ID:SGTC_2892|Compound:9047358|FD-Score:3.47|P-value:4.41E-5 ID:SGTC_2902|Compound:5787006|FD-Score:4.15|P-value:1.39E-6 ID:SGTC_2907|Compound:7721985|FD-Score:-3.58|P-value:3.02E-5 ID:SGTC_2942|Compound:9052777|FD-Score:4.93|P-value:1.32E-8 ID:SGTC_2945|Compound:9067681|FD-Score:4.77|P-value:3.87E-8 ID:SGTC_2948|Compound:9070189|FD-Score:-2.95|P-value:4.67E-4 ID:SGTC_3094|Compound:9117742|FD-Score:3.01|P-value:3.38E-4 ID:SGTC_3145|Compound:9085516|FD-Score:3.01|P-value:3.45E-4 ID:SGTC_3343|Compound:9148379|FD-Score:3.08|P-value:2.50E-4 ID:SGTC_3349|Compound:9149703|FD-Score:4.53|P-value:1.61E-7 ID:SGTC_3352|Compound:9151559|FD-Score:-3.19|P-value:1.78E-4 ID:SGTC_3353|Compound:9151644|FD-Score:-2.83|P-value:7.49E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:4.00|P-value:3.24E-6 ID:SGTC_78|Compound:0336-0123|FD-Score:-2.85|P-value:7.05E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:2.75|P-value:9.60E-4 ID:SGTC_282|Compound:k297-0036|FD-Score:2.88|P-value:5.78E-4 ID:SGTC_286|Compound:k060-0067|FD-Score:-2.81|P-value:8.15E-4 ID:SGTC_316|Compound:k060-0003|FD-Score:3.43|P-value:5.52E-5 ID:SGTC_341|Compound:1486-1430|FD-Score:-4.80|P-value:3.63E-8 ID:SGTC_343|Compound:4130-2827|FD-Score:-3.16|P-value:2.01E-4 ID:SGTC_347|Compound:4534-1311|FD-Score:-3.80|P-value:1.04E-5 ID:SGTC_349|Compound:k015-0014|FD-Score:-3.24|P-value:1.43E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:-4.40|P-value:3.98E-7 ID:SGTC_358|Compound:0075-0058|FD-Score:4.61|P-value:9.90E-8 ID:SGTC_407|Compound:eburnamonine|FD-Score:-3.31|P-value:1.02E-4 ID:SGTC_531|Compound:3788-1495|FD-Score:3.43|P-value:5.46E-5 ID:SGTC_561|Compound:1550-0330|FD-Score:3.25|P-value:1.22E-4 ID:SGTC_867|Compound:0518-0118|FD-Score:-4.27|P-value:8.43E-7 ID:SGTC_1020|Compound:4335-0961|FD-Score:-3.33|P-value:9.41E-5 ID:SGTC_1021|Compound:4476-0760|FD-Score:2.75|P-value:9.59E-4 ID:SGTC_1022|Compound:4477-0945|FD-Score:-2.85|P-value:7.15E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:3.01|P-value:3.37E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:3.16|P-value:1.83E-4 ID:SGTC_1497|Compound:4476-2978|FD-Score:-3.48|P-value:4.82E-5 ID:SGTC_1719|Compound:st032227|FD-Score:3.25|P-value:1.24E-4 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:3.13|P-value:2.09E-4 ID:SGTC_2025|Compound:5147754|FD-Score:3.73|P-value:1.27E-5 ID:SGTC_2102|Compound:5377816|FD-Score:-3.07|P-value:2.91E-4 ID:SGTC_2125|Compound:5246583|FD-Score:6.05|P-value:4.80E-12 ID:SGTC_2233|Compound:sulbentine|FD-Score:-2.96|P-value:4.60E-4 ID:SGTC_2274|Compound:7947094|FD-Score:-2.79|P-value:9.07E-4 ID:SGTC_2321|Compound:7390090|FD-Score:2.82|P-value:7.25E-4 ID:SGTC_2340|Compound:7987427|FD-Score:4.03|P-value:2.68E-6 ID:SGTC_2348|Compound:9022543|FD-Score:4.82|P-value:2.81E-8 ID:SGTC_2437|Compound:5741531|FD-Score:3.17|P-value:1.73E-4 ID:SGTC_2463|Compound:5473429|FD-Score:3.61|P-value:2.32E-5 ID:SGTC_2477|Compound:5967686|FD-Score:-3.13|P-value:2.27E-4 ID:SGTC_2478|Compound:5763493|FD-Score:4.16|P-value:1.34E-6 ID:SGTC_2489|Compound:cantharidin|FD-Score:-3.09|P-value:2.67E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:2.85|P-value:6.53E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-3.17|P-value:1.90E-4 ID:SGTC_2714|Compound:st079313|FD-Score:3.68|P-value:1.62E-5 ID:SGTC_2835|Compound:9006588|FD-Score:3.52|P-value:3.50E-5 ID:SGTC_2836|Compound:9010338|FD-Score:-2.90|P-value:5.78E-4 ID:SGTC_2840|Compound:9008771|FD-Score:-2.76|P-value:9.96E-4 ID:SGTC_2842|Compound:9000675|FD-Score:4.20|P-value:1.07E-6 ID:SGTC_2882|Compound:9058044|FD-Score:-2.99|P-value:4.10E-4 ID:SGTC_2892|Compound:9047358|FD-Score:3.47|P-value:4.41E-5 ID:SGTC_2902|Compound:5787006|FD-Score:4.15|P-value:1.39E-6 ID:SGTC_2907|Compound:7721985|FD-Score:-3.58|P-value:3.02E-5 ID:SGTC_2942|Compound:9052777|FD-Score:4.93|P-value:1.32E-8 ID:SGTC_2945|Compound:9067681|FD-Score:4.77|P-value:3.87E-8 ID:SGTC_2948|Compound:9070189|FD-Score:-2.95|P-value:4.67E-4 ID:SGTC_3094|Compound:9117742|FD-Score:3.01|P-value:3.38E-4 ID:SGTC_3145|Compound:9085516|FD-Score:3.01|P-value:3.45E-4 ID:SGTC_3343|Compound:9148379|FD-Score:3.08|P-value:2.50E-4 ID:SGTC_3349|Compound:9149703|FD-Score:4.53|P-value:1.61E-7 ID:SGTC_3352|Compound:9151559|FD-Score:-3.19|P-value:1.78E-4 ID:SGTC_3353|Compound:9151644|FD-Score:-2.83|P-value:7.49E-4

Top fitness defect scores for YKL103C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2125 5246583 6.05 200.0 µM 4.80E-12 tubulin folding & SWR complex 15 7.45E-10
2 SGTC_2942 9052777 4.93 62.2 µM 1.32E-8 11 4.01E-7
3 SGTC_2348 9022543 4.82 33.4 µM 2.81E-8 mitochondrial processes 15 7.30E-7
4 SGTC_2945 9067681 4.77 61.8 µM 3.87E-8 8 9.41E-7
5 SGTC_358 0075-0058 4.61 9.4 µM 9.90E-8 38 1.98E-6
6 SGTC_3349 9149703 4.53 23.0 µM 1.61E-7 9 2.92E-6
7 SGTC_2842 9000675 4.20 19.5 µM 1.07E-6 19 1.32E-5
8 SGTC_2478 5763493 4.16 32.3 µM 1.34E-6 21 1.58E-5
9 SGTC_2902 5787006 4.15 60.5 µM 1.39E-6 Golgi 23 1.63E-5
10 SGTC_2340 7987427 4.03 183.4 µM 2.68E-6 31 2.74E-5
11 SGTC_69 0096-0274 4.00 206.7 µM 3.24E-6 mitochondrial processes 39 3.19E-5
12 SGTC_2025 5147754 3.73 141.0 µM 1.27E-5 14 9.46E-5
13 SGTC_2714 st079313 3.68 56.9 µM 1.62E-5 12 1.15E-4
14 SGTC_2463 5473429 3.61 200.0 µM 2.32E-5 ERG2 25 1.53E-4
15 SGTC_2835 9006588 3.52 71.4 µM 3.50E-5 59 2.12E-4
16 SGTC_2892 9047358 3.47 58.4 µM 4.41E-5 17 2.56E-4
17 SGTC_531 3788-1495 3.43 174.0 µM 5.46E-5 unfolded protein response 34 3.03E-4
18 SGTC_316 k060-0003 3.43 58.6 µM 5.52E-5 25 3.06E-4
19 SGTC_561 1550-0330 3.25 72.9 µM 1.22E-4 31 5.78E-4
20 SGTC_1719 st032227 3.25 49.2 µM 1.24E-4 49 5.85E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.208 3.20E-34 YDL140C RPO21 het RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
0.153 6.30E-19 YKL066W_d YKL066W_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene YNK1
0.128 1.21E-13 YHR121W LSM12 hom Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress
0.123 7.23E-13 YCL075W YCL075W hom Pseudogene: encodes fragment of Ty Pol protein
0.099 1.00E-8 YNL162W-A_p YNL162W-A_p hom Putative protein of unknown function; identified by homology
0.096 2.54E-8 YOL078W AVO1 het Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth
0.093 7.33E-8 YNL152W INN1 het Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis
0.091 1.24E-7 YPL155C KIP2 hom Kinesin-related motor protein involved in mitotic spindle positioning, stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle
0.089 2.20E-7 YOR238W_p YOR238W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.089 2.78E-7 YDL222C FMP45 hom Integral membrane protein localized to mitochondria (untagged protein); required for sporulation and maintaining sphingolipid content; has sequence similarity to SUR7 and YNL194C
0.088 3.73E-7 YLR237W THI7 hom Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia
0.084 1.15E-6 YFL048C EMP47 hom Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport
0.083 1.62E-6 YHR131C_p YHR131C_p hom Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study
0.082 1.95E-6 YOR155C ISN1 hom Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms
0.080 3.95E-6 YKL171W NNK1 hom Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm