YKL124W / SSH4

Specificity factor required for Rsp5p-dependent ubiquitination and sorting of cargo proteins at the multivesicular body; identified as a high-copy suppressor of a SHR3 deletion, increasing steady-state levels of amino acid permeases

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL124W / SSH4

Click on Significant Values for Screen Details ID:SGTC_2|Compound:0422-0095|FD-Score:2.65|P-value:7.76E-4 ID:SGTC_51|Compound:1013-0126|FD-Score:2.82|P-value:3.82E-4 ID:SGTC_68|Compound:0986-0284|FD-Score:-4.64|P-value:2.99E-8 ID:SGTC_69|Compound:0096-0274|FD-Score:5.20|P-value:3.59E-10 ID:SGTC_70|Compound:0141-0289|FD-Score:-6.32|P-value:6.84E-14 ID:SGTC_72|Compound:0744-0168|FD-Score:4.85|P-value:4.26E-9 ID:SGTC_73|Compound:0327-0325|FD-Score:3.96|P-value:1.28E-6 ID:SGTC_75|Compound:0159-0059|FD-Score:3.52|P-value:1.40E-5 ID:SGTC_78|Compound:0336-0123|FD-Score:-6.73|P-value:1.68E-15 ID:SGTC_255|Compound:tunicamycin|FD-Score:2.66|P-value:7.53E-4 ID:SGTC_459|Compound:nigericin|FD-Score:3.59|P-value:9.77E-6 ID:SGTC_523|Compound:1934-0222|FD-Score:4.51|P-value:4.30E-8 ID:SGTC_673|Compound:1082-0474|FD-Score:2.90|P-value:2.75E-4 ID:SGTC_808|Compound:0711-0032|FD-Score:3.82|P-value:2.78E-6 ID:SGTC_848|Compound:0368-0089|FD-Score:2.70|P-value:6.25E-4 ID:SGTC_984|Compound:1492-0255|FD-Score:2.71|P-value:6.18E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:2.93|P-value:2.37E-4 ID:SGTC_1173|Compound:0443-0020|FD-Score:2.74|P-value:5.29E-4 ID:SGTC_1219|Compound:1469-0211|FD-Score:3.15|P-value:8.64E-5 ID:SGTC_1277|Compound:0873-0036|FD-Score:2.75|P-value:5.14E-4 ID:SGTC_1278|Compound:0874-0585|FD-Score:3.00|P-value:1.72E-4 ID:SGTC_1307|Compound:1187-1599|FD-Score:3.72|P-value:4.95E-6 ID:SGTC_1327|Compound:1436-0082|FD-Score:2.95|P-value:2.18E-4 ID:SGTC_1414|Compound:3917-0015|FD-Score:3.29|P-value:4.42E-5 ID:SGTC_1742|Compound:st038235|FD-Score:3.47|P-value:1.83E-5 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-2.79|P-value:5.99E-4 ID:SGTC_2127|Compound:5253358|FD-Score:3.28|P-value:4.76E-5 ID:SGTC_2129|Compound:girgensonine|FD-Score:2.92|P-value:2.48E-4 ID:SGTC_2255|Compound:7941087|FD-Score:3.18|P-value:7.77E-5 ID:SGTC_2265|Compound:7929201|FD-Score:-3.30|P-value:6.22E-5 ID:SGTC_2266|Compound:7929956|FD-Score:-5.30|P-value:2.80E-10 ID:SGTC_2267|Compound:7931562|FD-Score:-3.10|P-value:1.53E-4 ID:SGTC_2284|Compound:7945075|FD-Score:-2.76|P-value:6.82E-4 ID:SGTC_2307|Compound:7538926|FD-Score:-2.73|P-value:7.53E-4 ID:SGTC_2365|Compound:9042475|FD-Score:3.30|P-value:4.19E-5 ID:SGTC_2563|Compound:tomatine|FD-Score:4.56|P-value:3.08E-8 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:2.68|P-value:6.79E-4 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:2.67|P-value:7.28E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:2.71|P-value:6.08E-4 ID:SGTC_2730|Compound:imipramine|FD-Score:-3.29|P-value:6.24E-5 ID:SGTC_2801|Compound:7907800|FD-Score:-2.74|P-value:7.39E-4 ID:SGTC_2823|Compound:7987624|FD-Score:-3.75|P-value:6.06E-6 ID:SGTC_2853|Compound:9015042|FD-Score:-2.90|P-value:3.71E-4 ID:SGTC_2855|Compound:9016525|FD-Score:3.08|P-value:1.21E-4 ID:SGTC_2856|Compound:9022925|FD-Score:3.03|P-value:1.53E-4 ID:SGTC_2944|Compound:9067346|FD-Score:-2.84|P-value:4.84E-4 ID:SGTC_3022|Compound:9087993|FD-Score:2.70|P-value:6.28E-4 ID:SGTC_3032|Compound:9089427|FD-Score:-3.12|P-value:1.42E-4 ID:SGTC_3038|Compound:9091290|FD-Score:-2.99|P-value:2.49E-4 ID:SGTC_3083|Compound:9117465|FD-Score:-2.79|P-value:6.05E-4 ID:SGTC_3141|Compound:9094609|FD-Score:2.79|P-value:4.39E-4 ID:SGTC_3210|Compound:9128157|FD-Score:2.84|P-value:3.51E-4 ID:SGTC_3237|Compound:9133362|FD-Score:-2.76|P-value:6.71E-4 ID:SGTC_3260|Compound:9137109|FD-Score:2.75|P-value:5.27E-4 ID:SGTC_3344|Compound:9148862|FD-Score:-3.19|P-value:1.01E-4 ID:SGTC_3348|Compound:9149464|FD-Score:-2.78|P-value:6.17E-4 ID:SGTC_3351|Compound:9150312|FD-Score:-2.72|P-value:7.99E-4 ID:SGTC_2|Compound:0422-0095|FD-Score:2.65|P-value:7.76E-4 ID:SGTC_51|Compound:1013-0126|FD-Score:2.82|P-value:3.82E-4 ID:SGTC_68|Compound:0986-0284|FD-Score:-4.64|P-value:2.99E-8 ID:SGTC_69|Compound:0096-0274|FD-Score:5.20|P-value:3.59E-10 ID:SGTC_70|Compound:0141-0289|FD-Score:-6.32|P-value:6.84E-14 ID:SGTC_72|Compound:0744-0168|FD-Score:4.85|P-value:4.26E-9 ID:SGTC_73|Compound:0327-0325|FD-Score:3.96|P-value:1.28E-6 ID:SGTC_75|Compound:0159-0059|FD-Score:3.52|P-value:1.40E-5 ID:SGTC_78|Compound:0336-0123|FD-Score:-6.73|P-value:1.68E-15 ID:SGTC_255|Compound:tunicamycin|FD-Score:2.66|P-value:7.53E-4 ID:SGTC_459|Compound:nigericin|FD-Score:3.59|P-value:9.77E-6 ID:SGTC_523|Compound:1934-0222|FD-Score:4.51|P-value:4.30E-8 ID:SGTC_673|Compound:1082-0474|FD-Score:2.90|P-value:2.75E-4 ID:SGTC_808|Compound:0711-0032|FD-Score:3.82|P-value:2.78E-6 ID:SGTC_848|Compound:0368-0089|FD-Score:2.70|P-value:6.25E-4 ID:SGTC_984|Compound:1492-0255|FD-Score:2.71|P-value:6.18E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:2.93|P-value:2.37E-4 ID:SGTC_1173|Compound:0443-0020|FD-Score:2.74|P-value:5.29E-4 ID:SGTC_1219|Compound:1469-0211|FD-Score:3.15|P-value:8.64E-5 ID:SGTC_1277|Compound:0873-0036|FD-Score:2.75|P-value:5.14E-4 ID:SGTC_1278|Compound:0874-0585|FD-Score:3.00|P-value:1.72E-4 ID:SGTC_1307|Compound:1187-1599|FD-Score:3.72|P-value:4.95E-6 ID:SGTC_1327|Compound:1436-0082|FD-Score:2.95|P-value:2.18E-4 ID:SGTC_1414|Compound:3917-0015|FD-Score:3.29|P-value:4.42E-5 ID:SGTC_1742|Compound:st038235|FD-Score:3.47|P-value:1.83E-5 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-2.79|P-value:5.99E-4 ID:SGTC_2127|Compound:5253358|FD-Score:3.28|P-value:4.76E-5 ID:SGTC_2129|Compound:girgensonine|FD-Score:2.92|P-value:2.48E-4 ID:SGTC_2255|Compound:7941087|FD-Score:3.18|P-value:7.77E-5 ID:SGTC_2265|Compound:7929201|FD-Score:-3.30|P-value:6.22E-5 ID:SGTC_2266|Compound:7929956|FD-Score:-5.30|P-value:2.80E-10 ID:SGTC_2267|Compound:7931562|FD-Score:-3.10|P-value:1.53E-4 ID:SGTC_2284|Compound:7945075|FD-Score:-2.76|P-value:6.82E-4 ID:SGTC_2307|Compound:7538926|FD-Score:-2.73|P-value:7.53E-4 ID:SGTC_2365|Compound:9042475|FD-Score:3.30|P-value:4.19E-5 ID:SGTC_2563|Compound:tomatine|FD-Score:4.56|P-value:3.08E-8 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:2.68|P-value:6.79E-4 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:2.67|P-value:7.28E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:2.71|P-value:6.08E-4 ID:SGTC_2730|Compound:imipramine|FD-Score:-3.29|P-value:6.24E-5 ID:SGTC_2801|Compound:7907800|FD-Score:-2.74|P-value:7.39E-4 ID:SGTC_2823|Compound:7987624|FD-Score:-3.75|P-value:6.06E-6 ID:SGTC_2853|Compound:9015042|FD-Score:-2.90|P-value:3.71E-4 ID:SGTC_2855|Compound:9016525|FD-Score:3.08|P-value:1.21E-4 ID:SGTC_2856|Compound:9022925|FD-Score:3.03|P-value:1.53E-4 ID:SGTC_2944|Compound:9067346|FD-Score:-2.84|P-value:4.84E-4 ID:SGTC_3022|Compound:9087993|FD-Score:2.70|P-value:6.28E-4 ID:SGTC_3032|Compound:9089427|FD-Score:-3.12|P-value:1.42E-4 ID:SGTC_3038|Compound:9091290|FD-Score:-2.99|P-value:2.49E-4 ID:SGTC_3083|Compound:9117465|FD-Score:-2.79|P-value:6.05E-4 ID:SGTC_3141|Compound:9094609|FD-Score:2.79|P-value:4.39E-4 ID:SGTC_3210|Compound:9128157|FD-Score:2.84|P-value:3.51E-4 ID:SGTC_3237|Compound:9133362|FD-Score:-2.76|P-value:6.71E-4 ID:SGTC_3260|Compound:9137109|FD-Score:2.75|P-value:5.27E-4 ID:SGTC_3344|Compound:9148862|FD-Score:-3.19|P-value:1.01E-4 ID:SGTC_3348|Compound:9149464|FD-Score:-2.78|P-value:6.17E-4 ID:SGTC_3351|Compound:9150312|FD-Score:-2.72|P-value:7.99E-4

Top fitness defect scores for YKL124W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_69 0096-0274 5.20 206.7 µM 3.59E-10 mitochondrial processes 14 1.01E-7
2 SGTC_72 0744-0168 4.85 198.4 µM 4.26E-9 RPP1 & pyrimidine depletion 18 6.05E-7
3 SGTC_2563 tomatine 4.56 8.5 µM 3.08E-8 26 2.53E-6
4 SGTC_523 1934-0222 4.51 13.7 µM 4.30E-8 21 3.23E-6
5 SGTC_73 0327-0325 3.96 16.2 µM 1.28E-6 DNA damage response 51 3.78E-5
6 SGTC_808 0711-0032 3.82 135.0 µM 2.78E-6 15 6.63E-5
7 SGTC_1307 1187-1599 3.72 12.2 µM 4.95E-6 PDR1 15 1.01E-4
8 SGTC_459 nigericin 3.59 15.3 µM 9.77E-6 75 1.65E-4
9 SGTC_75 0159-0059 3.52 199.0 µM 1.40E-5 fatty acid desaturase (OLE1) 29 2.14E-4
10 SGTC_1742 st038235 3.47 28.9 µM 1.83E-5 mitochondrial stress 141 2.61E-4
11 SGTC_2365 9042475 3.30 200.0 µM 4.19E-5 71 4.76E-4
12 SGTC_1414 3917-0015 3.29 5.4 µM 4.42E-5 24 4.95E-4
13 SGTC_2127 5253358 3.28 187.9 µM 4.76E-5 46 5.23E-4
14 SGTC_2255 7941087 3.18 197.4 µM 7.77E-5 62 7.47E-4
15 SGTC_1219 1469-0211 3.15 33.1 µM 8.64E-5 38 8.07E-4
16 SGTC_2855 9016525 3.08 58.4 µM 1.21E-4 57 0.00103
17 SGTC_2856 9022925 3.03 19.5 µM 1.53E-4 71 0.00122
18 SGTC_1278 0874-0585 3.00 172.0 µM 1.72E-4 53 0.00133
19 SGTC_1327 1436-0082 2.95 2.2 µM 2.18E-4 62 0.00158
20 SGTC_1072 oxiconazole 2.93 14.9 nM 2.37E-4 ergosterol depletion effects on membrane 97 0.00168

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.176 1.10E-24 YGL042C_d YGL042C_d hom Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1
0.148 7.02E-18 YPR195C_d YPR195C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.131 2.76E-14 YPL213W LEA1 hom Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein
0.127 1.87E-13 YLR272C YCS4 het Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
0.125 3.45E-13 YMR221C_p YMR221C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy
0.123 8.68E-13 YER043C SAH1 het S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor
0.116 1.56E-11 YOR154W_p SLP1_p hom Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p
0.107 5.44E-10 YNR032C-A HUB1 hom Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear
0.106 7.38E-10 YJR153W PGU1 hom Endo-polygalacturonase, pectolytic enzyme that hydrolyzes the alpha-1,4-glycosidic bonds in the rhamnogalacturonan chains in pectins
0.104 1.34E-9 YFR054C_d YFR054C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.102 3.62E-9 YPR121W THI22 hom Protein with similarity to hydroxymethylpyrimidine phosphate kinases; member of a gene family with THI20 and THI21; not required for thiamine biosynthesis
0.099 9.19E-9 YOR289W_p YOR289W_p hom Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
0.099 9.01E-9 YGR209C TRX2 hom Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress
0.098 1.35E-8 YKL197C PEX1 hom AAA-peroxin that heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis
0.093 6.78E-8 YBR097W VPS15 hom Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery