HIPHOP chemogenomics database

YKL124W / SSH4

Specificity factor required for Rsp5p-dependent ubiquitination and sorting of cargo proteins at the multivesicular body; identified as a high-copy suppressor of a SHR3 deletion, increasing steady-state levels of amino acid permeases

Zygosity: Homozygous strain

fixedexpanded
Profile for YKL124W / SSH4

Click on Significant Values for Screen Details

Top fitness defect scores for YKL124W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_69	0096-0274	5.20	206.7 µM	3.59E-10	mitochondrial processes	14	1.01E-7
2	SGTC_72	0744-0168	4.85	198.4 µM	4.26E-9	RPP1 & pyrimidine depletion	18	6.05E-7
3	SGTC_2563	tomatine	4.56	8.5 µM	3.08E-8		26	2.53E-6
4	SGTC_523	1934-0222	4.51	13.7 µM	4.30E-8		21	3.23E-6
5	SGTC_73	0327-0325	3.96	16.2 µM	1.28E-6	DNA damage response	51	3.78E-5
6	SGTC_808	0711-0032	3.82	135.0 µM	2.78E-6		15	6.63E-5
7	SGTC_1307	1187-1599	3.72	12.2 µM	4.95E-6	PDR1	15	1.01E-4
8	SGTC_459	nigericin	3.59	15.3 µM	9.77E-6		75	1.65E-4
9	SGTC_75	0159-0059	3.52	199.0 µM	1.40E-5	fatty acid desaturase (OLE1)	29	2.14E-4
10	SGTC_1742	st038235	3.47	28.9 µM	1.83E-5	mitochondrial stress	141	2.61E-4
11	SGTC_2365	9042475	3.30	200.0 µM	4.19E-5		71	4.76E-4
12	SGTC_1414	3917-0015	3.29	5.4 µM	4.42E-5		24	4.95E-4
13	SGTC_2127	5253358	3.28	187.9 µM	4.76E-5		46	5.23E-4
14	SGTC_2255	7941087	3.18	197.4 µM	7.77E-5		62	7.47E-4
15	SGTC_1219	1469-0211	3.15	33.1 µM	8.64E-5		38	8.07E-4
16	SGTC_2855	9016525	3.08	58.4 µM	1.21E-4		57	0.00103
17	SGTC_2856	9022925	3.03	19.5 µM	1.53E-4		71	0.00122
18	SGTC_1278	0874-0585	3.00	172.0 µM	1.72E-4		53	0.00133
19	SGTC_1327	1436-0082	2.95	2.2 µM	2.18E-4		62	0.00158
20	SGTC_1072	oxiconazole	2.93	14.9 nM	2.37E-4	ergosterol depletion effects on membrane	97	0.00168

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.176	1.10E-24	YGL042C_d	YGL042C_d	hom	Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1
0.148	7.02E-18	YPR195C_d	YPR195C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.131	2.76E-14	YPL213W	LEA1	hom	Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein
0.127	1.87E-13	YLR272C	YCS4	het	Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
0.125	3.45E-13	YMR221C_p	YMR221C_p	hom	Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy
0.123	8.68E-13	YER043C	SAH1	het	S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor
0.116	1.56E-11	YOR154W_p	SLP1_p	hom	Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p
0.107	5.44E-10	YNR032C-A	HUB1	hom	Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear
0.106	7.38E-10	YJR153W	PGU1	hom	Endo-polygalacturonase, pectolytic enzyme that hydrolyzes the alpha-1,4-glycosidic bonds in the rhamnogalacturonan chains in pectins
0.104	1.34E-9	YFR054C_d	YFR054C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.102	3.62E-9	YPR121W	THI22	hom	Protein with similarity to hydroxymethylpyrimidine phosphate kinases; member of a gene family with THI20 and THI21; not required for thiamine biosynthesis
0.099	9.19E-9	YOR289W_p	YOR289W_p	hom	Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
0.099	9.01E-9	YGR209C	TRX2	hom	Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress
0.098	1.35E-8	YKL197C	PEX1	hom	AAA-peroxin that heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis
0.093	6.78E-8	YBR097W	VPS15	hom	Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery