YKL144C / RPC25

RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKL144C / RPC25

Click on Significant Values for Screen Details ID:SGTC_127|Compound:0180-0333|FD-Score:-3.56|P-value:5.99E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:6.39|P-value:3.66E-10 ID:SGTC_313|Compound:3474-0040|FD-Score:3.53|P-value:2.71E-4 ID:SGTC_558|Compound:k064-0027|FD-Score:5.46|P-value:6.39E-8 ID:SGTC_667|Compound:1498-1044|FD-Score:3.61|P-value:2.06E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:-3.63|P-value:4.77E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:3.45|P-value:3.59E-4 ID:SGTC_691|Compound:1548-0686|FD-Score:3.32|P-value:5.47E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:-4.14|P-value:7.68E-5 ID:SGTC_1022|Compound:4477-0945|FD-Score:-3.50|P-value:7.32E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:3.81|P-value:9.84E-5 ID:SGTC_1272|Compound:0839-0021|FD-Score:4.09|P-value:3.37E-5 ID:SGTC_1747|Compound:st037496|FD-Score:3.63|P-value:1.93E-4 ID:SGTC_1915|Compound:st067105|FD-Score:3.73|P-value:1.31E-4 ID:SGTC_2129|Compound:girgensonine|FD-Score:-3.77|P-value:2.99E-4 ID:SGTC_2316|Compound:5844804|FD-Score:-4.23|P-value:5.52E-5 ID:SGTC_2411|Compound:st077133|FD-Score:4.63|P-value:3.41E-6 ID:SGTC_2450|Compound:5739737|FD-Score:4.20|P-value:2.10E-5 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:3.81|P-value:9.72E-5 ID:SGTC_2711|Compound:st079103|FD-Score:-3.72|P-value:3.54E-4 ID:SGTC_2835|Compound:9006588|FD-Score:4.68|P-value:2.74E-6 ID:SGTC_2854|Compound:9024559|FD-Score:-3.95|P-value:1.54E-4 ID:SGTC_2948|Compound:9070189|FD-Score:-3.48|P-value:7.81E-4 ID:SGTC_2967|Compound:9089538|FD-Score:-3.41|P-value:9.83E-4 ID:SGTC_3215|Compound:9129942|FD-Score:-3.88|P-value:2.01E-4 ID:SGTC_3272|Compound:9137413|FD-Score:3.29|P-value:6.22E-4 ID:SGTC_127|Compound:0180-0333|FD-Score:-3.56|P-value:5.99E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:6.39|P-value:3.66E-10 ID:SGTC_313|Compound:3474-0040|FD-Score:3.53|P-value:2.71E-4 ID:SGTC_558|Compound:k064-0027|FD-Score:5.46|P-value:6.39E-8 ID:SGTC_667|Compound:1498-1044|FD-Score:3.61|P-value:2.06E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:-3.63|P-value:4.77E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:3.45|P-value:3.59E-4 ID:SGTC_691|Compound:1548-0686|FD-Score:3.32|P-value:5.47E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:-4.14|P-value:7.68E-5 ID:SGTC_1022|Compound:4477-0945|FD-Score:-3.50|P-value:7.32E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:3.81|P-value:9.84E-5 ID:SGTC_1272|Compound:0839-0021|FD-Score:4.09|P-value:3.37E-5 ID:SGTC_1747|Compound:st037496|FD-Score:3.63|P-value:1.93E-4 ID:SGTC_1915|Compound:st067105|FD-Score:3.73|P-value:1.31E-4 ID:SGTC_2129|Compound:girgensonine|FD-Score:-3.77|P-value:2.99E-4 ID:SGTC_2316|Compound:5844804|FD-Score:-4.23|P-value:5.52E-5 ID:SGTC_2411|Compound:st077133|FD-Score:4.63|P-value:3.41E-6 ID:SGTC_2450|Compound:5739737|FD-Score:4.20|P-value:2.10E-5 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:3.81|P-value:9.72E-5 ID:SGTC_2711|Compound:st079103|FD-Score:-3.72|P-value:3.54E-4 ID:SGTC_2835|Compound:9006588|FD-Score:4.68|P-value:2.74E-6 ID:SGTC_2854|Compound:9024559|FD-Score:-3.95|P-value:1.54E-4 ID:SGTC_2948|Compound:9070189|FD-Score:-3.48|P-value:7.81E-4 ID:SGTC_2967|Compound:9089538|FD-Score:-3.41|P-value:9.83E-4 ID:SGTC_3215|Compound:9129942|FD-Score:-3.88|P-value:2.01E-4 ID:SGTC_3272|Compound:9137413|FD-Score:3.29|P-value:6.22E-4

Top fitness defect scores for YKL144C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_138 0850-0259 6.39 40.9 µM 3.66E-10 cell wall 28 8.38E-11 0.04
2 SGTC_558 k064-0027 5.46 21.3 µM 6.39E-8 DNA damage response 4 2.38E-8 0.61
3 SGTC_2835 9006588 4.68 71.4 µM 2.74E-6 5 1.44E-6 0.05
4 SGTC_2411 st077133 4.63 39.9 µM 3.41E-6 RNA pol III & RNase P/MRP 16 1.82E-6 0.43
5 SGTC_2450 5739737 4.20 12.9 µM 2.10E-5 3 1.31E-5 0.30
6 SGTC_1272 0839-0021 4.09 27.0 µM 3.37E-5 6 2.19E-5 0.10
7 SGTC_2538 3',7'-epoxycaryophyllan-6-one 3.81 100.0 µM 9.72E-5 12 6.88E-5 0.15
8 SGTC_1133 3473-0247 3.81 50.0 µM 9.84E-5 RSC complex & mRNA processing 50 6.98E-5 0.02
9 SGTC_1915 st067105 3.73 30.6 µM 1.31E-4 RNA pol III & RNase P/MRP 38 9.52E-5 0.31
10 SGTC_1747 st037496 3.63 66.6 µM 1.93E-4 11 1.44E-4 0.00
11 SGTC_667 1498-1044 3.61 59.0 µM 2.06E-4 RPP1 & pyrimidine depletion 10 1.55E-4 0.03
12 SGTC_313 3474-0040 3.53 5.0 µM 2.71E-4 NEO1-PIK1 10 2.08E-4 0.04
13 SGTC_673 1082-0474 3.45 110.0 µM 3.59E-4 RSC complex & mRNA processing 64 2.81E-4 0.07
14 SGTC_691 1548-0686 3.32 185.0 µM 5.47E-4 20 4.43E-4 0.07
15 SGTC_3272 9137413 3.29 49.5 µM 6.22E-4 16 5.09E-4 0.07
16 SGTC_1960 st011366 3.04 51.8 µM 0.00138 18 0.00119 0.09
17 SGTC_3274 9138298 3.02 49.5 µM 0.00147 13 0.00128 0.02
18 SGTC_2134 5324836 2.99 1.5 µM 0.00161 10 0.00142 0.15
19 SGTC_1910 st062352 2.96 60.4 µM 0.00174 12 0.00153 0.09
20 SGTC_552 k242-0005 2.96 476.0 µM 0.00175 10 0.00154 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.204 5.17E-33 YDL152W_d YDL152W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
0.193 1.50E-29 YJL011C RPC17 het RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress
0.166 4.90E-22 YNL271C BNI1 hom Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1
0.164 9.25E-22 YDR016C DAD1 het Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
0.157 6.05E-20 YPR033C HTS1 het Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome
0.155 1.65E-19 YBR091C TIM12 het Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
0.150 2.79E-18 YOR116C RPO31 het RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
0.147 8.81E-18 YDR540C IRC4 hom Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
0.146 1.86E-17 YPL242C IQG1 het Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.140 3.08E-16 YLL021W SPA2 hom Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate
0.135 4.13E-15 YLR150W STM1 hom Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
0.124 6.64E-13 YOR051C ETT1 hom Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough
0.122 1.47E-12 YJL080C SCP160 hom Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins
0.122 1.63E-12 YNL221C POP1 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
0.119 4.18E-12 YPL269W KAR9 hom Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase