YKL166C / TPK3

cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk2p; localizes to P-bodies during stationary phase; TPK3 has a paralog, TPK1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL166C / TPK3

Click on Significant Values for Screen Details ID:SGTC_407|Compound:eburnamonine|FD-Score:-2.75|P-value:8.91E-4 ID:SGTC_830|Compound:4076-0286|FD-Score:2.90|P-value:5.09E-4 ID:SGTC_1208|Compound:2323-2347|FD-Score:2.80|P-value:7.41E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:3.04|P-value:2.85E-4 ID:SGTC_1259|Compound:0671-0132|FD-Score:2.78|P-value:7.96E-4 ID:SGTC_1262|Compound:0737-0114|FD-Score:2.94|P-value:4.20E-4 ID:SGTC_1300|Compound:1094-0077|FD-Score:-2.94|P-value:4.12E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-5.55|P-value:1.52E-10 ID:SGTC_1463|Compound:k081-0032|FD-Score:-4.07|P-value:1.90E-6 ID:SGTC_1472|Compound:k841-0093|FD-Score:3.16|P-value:1.66E-4 ID:SGTC_1613|Compound:st002381|FD-Score:-3.68|P-value:1.49E-5 ID:SGTC_1708|Compound:st033234|FD-Score:4.09|P-value:1.76E-6 ID:SGTC_1948|Compound:st076329|FD-Score:3.26|P-value:1.09E-4 ID:SGTC_2092|Compound:5303694|FD-Score:-3.45|P-value:4.50E-5 ID:SGTC_2098|Compound:5376439|FD-Score:2.86|P-value:5.96E-4 ID:SGTC_2100|Compound:5377183|FD-Score:3.41|P-value:5.48E-5 ID:SGTC_2103|Compound:5378138|FD-Score:4.19|P-value:1.00E-6 ID:SGTC_2113|Compound:5491891|FD-Score:-2.80|P-value:7.32E-4 ID:SGTC_2321|Compound:7390090|FD-Score:-5.60|P-value:1.01E-10 ID:SGTC_2325|Compound:6158266|FD-Score:2.91|P-value:4.79E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:3.25|P-value:1.16E-4 ID:SGTC_2759|Compound:disulfiram|FD-Score:-5.46|P-value:2.87E-10 ID:SGTC_2816|Compound:7982706|FD-Score:3.24|P-value:1.21E-4 ID:SGTC_2887|Compound:9044784|FD-Score:-2.99|P-value:3.39E-4 ID:SGTC_3125|Compound:9127534|FD-Score:2.77|P-value:8.49E-4 ID:SGTC_3129|Compound:9127257|FD-Score:-3.17|P-value:1.59E-4 ID:SGTC_3192|Compound:9110362|FD-Score:-3.23|P-value:1.24E-4 ID:SGTC_3209|Compound:9113616|FD-Score:2.94|P-value:4.27E-4 ID:SGTC_407|Compound:eburnamonine|FD-Score:-2.75|P-value:8.91E-4 ID:SGTC_830|Compound:4076-0286|FD-Score:2.90|P-value:5.09E-4 ID:SGTC_1208|Compound:2323-2347|FD-Score:2.80|P-value:7.41E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:3.04|P-value:2.85E-4 ID:SGTC_1259|Compound:0671-0132|FD-Score:2.78|P-value:7.96E-4 ID:SGTC_1262|Compound:0737-0114|FD-Score:2.94|P-value:4.20E-4 ID:SGTC_1300|Compound:1094-0077|FD-Score:-2.94|P-value:4.12E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-5.55|P-value:1.52E-10 ID:SGTC_1463|Compound:k081-0032|FD-Score:-4.07|P-value:1.90E-6 ID:SGTC_1472|Compound:k841-0093|FD-Score:3.16|P-value:1.66E-4 ID:SGTC_1613|Compound:st002381|FD-Score:-3.68|P-value:1.49E-5 ID:SGTC_1708|Compound:st033234|FD-Score:4.09|P-value:1.76E-6 ID:SGTC_1948|Compound:st076329|FD-Score:3.26|P-value:1.09E-4 ID:SGTC_2092|Compound:5303694|FD-Score:-3.45|P-value:4.50E-5 ID:SGTC_2098|Compound:5376439|FD-Score:2.86|P-value:5.96E-4 ID:SGTC_2100|Compound:5377183|FD-Score:3.41|P-value:5.48E-5 ID:SGTC_2103|Compound:5378138|FD-Score:4.19|P-value:1.00E-6 ID:SGTC_2113|Compound:5491891|FD-Score:-2.80|P-value:7.32E-4 ID:SGTC_2321|Compound:7390090|FD-Score:-5.60|P-value:1.01E-10 ID:SGTC_2325|Compound:6158266|FD-Score:2.91|P-value:4.79E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:3.25|P-value:1.16E-4 ID:SGTC_2759|Compound:disulfiram|FD-Score:-5.46|P-value:2.87E-10 ID:SGTC_2816|Compound:7982706|FD-Score:3.24|P-value:1.21E-4 ID:SGTC_2887|Compound:9044784|FD-Score:-2.99|P-value:3.39E-4 ID:SGTC_3125|Compound:9127534|FD-Score:2.77|P-value:8.49E-4 ID:SGTC_3129|Compound:9127257|FD-Score:-3.17|P-value:1.59E-4 ID:SGTC_3192|Compound:9110362|FD-Score:-3.23|P-value:1.24E-4 ID:SGTC_3209|Compound:9113616|FD-Score:2.94|P-value:4.27E-4

Top fitness defect scores for YKL166C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2103 5378138 4.19 10.0 µM 1.00E-6 11 1.40E-5
2 SGTC_1708 st033234 4.09 31.8 µM 1.76E-6 15 2.18E-5
3 SGTC_2100 5377183 3.41 190.0 µM 5.48E-5 60S ribosome export 22 3.25E-4
4 SGTC_1948 st076329 3.26 66.6 µM 1.09E-4 55 5.59E-4
5 SGTC_2754 granisetron 3.25 64.0 µM 1.16E-4 68 5.86E-4
6 SGTC_2816 7982706 3.24 58.4 µM 1.21E-4 azole & statin 88 6.08E-4
7 SGTC_1472 k841-0093 3.16 193.0 µM 1.66E-4 46 7.81E-4
8 SGTC_1254 0550-0035 3.04 264.0 µM 2.85E-4 61 0.00119
9 SGTC_1262 0737-0114 2.94 49.6 µM 4.20E-4 70 0.00162
10 SGTC_3209 9113616 2.94 49.5 µM 4.27E-4 87 0.00164
11 SGTC_2325 6158266 2.91 200.0 µM 4.79E-4 73 0.00180
12 SGTC_830 4076-0286 2.90 57.8 µM 5.09E-4 translation 166 0.00189
13 SGTC_2098 5376439 2.86 882.5 nM 5.96E-4 53 0.00214
14 SGTC_1208 2323-2347 2.80 50.3 µM 7.41E-4 54 0.00254
15 SGTC_1259 0671-0132 2.78 43.0 µM 7.96E-4 88 0.00269
16 SGTC_3125 9127534 2.77 49.5 µM 8.49E-4 42 0.00283
17 SGTC_321 4534-3890 2.69 65.3 µM 0.00112 RPP1 & pyrimidine depletion 48 0.00352
18 SGTC_234 nsc-17383 2.68 10.7 µM 0.00119 ERG2 101 0.00371
19 SGTC_65 0798-0905 2.63 35.0 µM 0.00144 calcium & mitochondrial duress 203 0.00430
20 SGTC_3212 9128307 2.62 49.5 µM 0.00147 131 0.00436

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.365 5.10E-106 YIL010W DOT5 hom Nuclear thiol peroxidase which functions as an alkyl-hydroperoxide reductase during post-diauxic growth
0.301 2.90E-71 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.299 5.03E-70 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.297 3.05E-69 YJR146W_p YJR146W_p hom Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2
0.291 3.78E-66 YDL176W YDL176W hom Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene
0.285 1.51E-63 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.280 2.97E-61 YLR179C YLR179C hom Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus
0.276 5.08E-59 YPR180W AOS1 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.271 1.60E-57 YDR521W_d YDR521W_d hom Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.261 2.92E-53 YGR077C PEX8 hom Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.251 3.16E-49 YDL157C_p YDL157C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.237 4.31E-44 YDL197C ASF2 hom Anti-silencing protein that causes derepression of silent loci when overexpressed
0.237 5.52E-44 YDR312W SSF2 hom Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication
0.233 2.14E-42 YMR137C PSO2 hom Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress
0.232 3.56E-42 YDR169C STB3 hom Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress