YKR003W / OSH6

Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion

Zygosity: Homozygous strain
fixedexpanded
Profile for YKR003W / OSH6

Click on Significant Values for Screen Details ID:SGTC_131|Compound:0141-0277|FD-Score:3.10|P-value:5.46E-4 ID:SGTC_285|Compound:1486-1325|FD-Score:3.22|P-value:3.50E-4 ID:SGTC_399|Compound:oxolinic acid|FD-Score:3.48|P-value:1.25E-4 ID:SGTC_550|Compound:4123-0385|FD-Score:3.77|P-value:3.81E-5 ID:SGTC_625|Compound:k213-0058|FD-Score:3.14|P-value:4.66E-4 ID:SGTC_817|Compound:1391-0715|FD-Score:3.41|P-value:1.70E-4 ID:SGTC_828|Compound:2074-0507|FD-Score:4.90|P-value:1.44E-7 ID:SGTC_925|Compound:2064-1272|FD-Score:4.05|P-value:1.05E-5 ID:SGTC_933|Compound:2914-0560|FD-Score:3.08|P-value:5.83E-4 ID:SGTC_1011|Compound:4112-3381|FD-Score:4.26|P-value:4.01E-6 ID:SGTC_1032|Compound:k061-1018|FD-Score:4.55|P-value:9.60E-7 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.50|P-value:1.16E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.43|P-value:1.57E-4 ID:SGTC_1118|Compound:chelidonine|FD-Score:4.85|P-value:1.90E-7 ID:SGTC_1151|Compound:3909-8316|FD-Score:3.42|P-value:1.62E-4 ID:SGTC_1218|Compound:1348-1416|FD-Score:-3.11|P-value:7.68E-4 ID:SGTC_1232|Compound:0279-0259|FD-Score:-3.76|P-value:6.05E-5 ID:SGTC_1247|Compound:0448-0043|FD-Score:3.62|P-value:7.19E-5 ID:SGTC_1302|Compound:1159-0335|FD-Score:3.95|P-value:1.67E-5 ID:SGTC_1554|Compound:st069299|FD-Score:4.18|P-value:5.76E-6 ID:SGTC_1602|Compound:st001878|FD-Score:3.77|P-value:3.79E-5 ID:SGTC_1607|Compound:st000305|FD-Score:3.11|P-value:5.34E-4 ID:SGTC_1747|Compound:st037496|FD-Score:3.36|P-value:2.05E-4 ID:SGTC_1866|Compound:st057638|FD-Score:2.95|P-value:9.45E-4 ID:SGTC_1940|Compound:st075990|FD-Score:-3.78|P-value:5.49E-5 ID:SGTC_1945|Compound:st075991|FD-Score:-4.22|P-value:7.74E-6 ID:SGTC_2030|Compound:5175110|FD-Score:4.11|P-value:8.28E-6 ID:SGTC_2135|Compound:5326629|FD-Score:-3.12|P-value:7.43E-4 ID:SGTC_2171|Compound:5739187|FD-Score:3.05|P-value:6.69E-4 ID:SGTC_2247|Compound:cerulenin|FD-Score:-3.18|P-value:5.90E-4 ID:SGTC_2259|Compound:7964009|FD-Score:3.43|P-value:1.54E-4 ID:SGTC_2303|Compound:7774051|FD-Score:-3.04|P-value:9.93E-4 ID:SGTC_2336|Compound:9001819|FD-Score:-4.01|P-value:2.06E-5 ID:SGTC_2416|Compound:tofa|FD-Score:3.20|P-value:3.86E-4 ID:SGTC_2464|Compound:5717415|FD-Score:3.04|P-value:6.87E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:-3.08|P-value:8.48E-4 ID:SGTC_2594|Compound:a-mangostin|FD-Score:6.25|P-value:3.45E-11 ID:SGTC_2647|Compound:atorvastatin|FD-Score:-3.38|P-value:2.84E-4 ID:SGTC_2675|Compound:theaflavin digallate|FD-Score:3.07|P-value:6.17E-4 ID:SGTC_2676|Compound:harmalol|FD-Score:3.63|P-value:6.94E-5 ID:SGTC_2782|Compound:7736369|FD-Score:3.26|P-value:3.07E-4 ID:SGTC_2827|Compound:7993847|FD-Score:3.12|P-value:5.12E-4 ID:SGTC_2913|Compound:7966556|FD-Score:3.28|P-value:2.80E-4 ID:SGTC_2930|Compound:9013230|FD-Score:4.57|P-value:8.31E-7 ID:SGTC_2932|Compound:9016711|FD-Score:5.61|P-value:2.20E-9 ID:SGTC_2952|Compound:9073731|FD-Score:3.18|P-value:4.15E-4 ID:SGTC_2966|Compound:9084041|FD-Score:3.99|P-value:1.43E-5 ID:SGTC_2974|Compound:9094440|FD-Score:-3.92|P-value:3.08E-5 ID:SGTC_3132|Compound:9126706|FD-Score:4.16|P-value:6.54E-6 ID:SGTC_3305|Compound:9127027|FD-Score:5.19|P-value:2.91E-8 ID:SGTC_3324|Compound:9138759|FD-Score:-3.67|P-value:8.78E-5 ID:SGTC_3330|Compound:9141424|FD-Score:3.80|P-value:3.37E-5 ID:SGTC_131|Compound:0141-0277|FD-Score:3.10|P-value:5.46E-4 ID:SGTC_285|Compound:1486-1325|FD-Score:3.22|P-value:3.50E-4 ID:SGTC_399|Compound:oxolinic acid|FD-Score:3.48|P-value:1.25E-4 ID:SGTC_550|Compound:4123-0385|FD-Score:3.77|P-value:3.81E-5 ID:SGTC_625|Compound:k213-0058|FD-Score:3.14|P-value:4.66E-4 ID:SGTC_817|Compound:1391-0715|FD-Score:3.41|P-value:1.70E-4 ID:SGTC_828|Compound:2074-0507|FD-Score:4.90|P-value:1.44E-7 ID:SGTC_925|Compound:2064-1272|FD-Score:4.05|P-value:1.05E-5 ID:SGTC_933|Compound:2914-0560|FD-Score:3.08|P-value:5.83E-4 ID:SGTC_1011|Compound:4112-3381|FD-Score:4.26|P-value:4.01E-6 ID:SGTC_1032|Compound:k061-1018|FD-Score:4.55|P-value:9.60E-7 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.50|P-value:1.16E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.43|P-value:1.57E-4 ID:SGTC_1118|Compound:chelidonine|FD-Score:4.85|P-value:1.90E-7 ID:SGTC_1151|Compound:3909-8316|FD-Score:3.42|P-value:1.62E-4 ID:SGTC_1218|Compound:1348-1416|FD-Score:-3.11|P-value:7.68E-4 ID:SGTC_1232|Compound:0279-0259|FD-Score:-3.76|P-value:6.05E-5 ID:SGTC_1247|Compound:0448-0043|FD-Score:3.62|P-value:7.19E-5 ID:SGTC_1302|Compound:1159-0335|FD-Score:3.95|P-value:1.67E-5 ID:SGTC_1554|Compound:st069299|FD-Score:4.18|P-value:5.76E-6 ID:SGTC_1602|Compound:st001878|FD-Score:3.77|P-value:3.79E-5 ID:SGTC_1607|Compound:st000305|FD-Score:3.11|P-value:5.34E-4 ID:SGTC_1747|Compound:st037496|FD-Score:3.36|P-value:2.05E-4 ID:SGTC_1866|Compound:st057638|FD-Score:2.95|P-value:9.45E-4 ID:SGTC_1940|Compound:st075990|FD-Score:-3.78|P-value:5.49E-5 ID:SGTC_1945|Compound:st075991|FD-Score:-4.22|P-value:7.74E-6 ID:SGTC_2030|Compound:5175110|FD-Score:4.11|P-value:8.28E-6 ID:SGTC_2135|Compound:5326629|FD-Score:-3.12|P-value:7.43E-4 ID:SGTC_2171|Compound:5739187|FD-Score:3.05|P-value:6.69E-4 ID:SGTC_2247|Compound:cerulenin|FD-Score:-3.18|P-value:5.90E-4 ID:SGTC_2259|Compound:7964009|FD-Score:3.43|P-value:1.54E-4 ID:SGTC_2303|Compound:7774051|FD-Score:-3.04|P-value:9.93E-4 ID:SGTC_2336|Compound:9001819|FD-Score:-4.01|P-value:2.06E-5 ID:SGTC_2416|Compound:tofa|FD-Score:3.20|P-value:3.86E-4 ID:SGTC_2464|Compound:5717415|FD-Score:3.04|P-value:6.87E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:-3.08|P-value:8.48E-4 ID:SGTC_2594|Compound:a-mangostin|FD-Score:6.25|P-value:3.45E-11 ID:SGTC_2647|Compound:atorvastatin|FD-Score:-3.38|P-value:2.84E-4 ID:SGTC_2675|Compound:theaflavin digallate|FD-Score:3.07|P-value:6.17E-4 ID:SGTC_2676|Compound:harmalol|FD-Score:3.63|P-value:6.94E-5 ID:SGTC_2782|Compound:7736369|FD-Score:3.26|P-value:3.07E-4 ID:SGTC_2827|Compound:7993847|FD-Score:3.12|P-value:5.12E-4 ID:SGTC_2913|Compound:7966556|FD-Score:3.28|P-value:2.80E-4 ID:SGTC_2930|Compound:9013230|FD-Score:4.57|P-value:8.31E-7 ID:SGTC_2932|Compound:9016711|FD-Score:5.61|P-value:2.20E-9 ID:SGTC_2952|Compound:9073731|FD-Score:3.18|P-value:4.15E-4 ID:SGTC_2966|Compound:9084041|FD-Score:3.99|P-value:1.43E-5 ID:SGTC_2974|Compound:9094440|FD-Score:-3.92|P-value:3.08E-5 ID:SGTC_3132|Compound:9126706|FD-Score:4.16|P-value:6.54E-6 ID:SGTC_3305|Compound:9127027|FD-Score:5.19|P-value:2.91E-8 ID:SGTC_3324|Compound:9138759|FD-Score:-3.67|P-value:8.78E-5 ID:SGTC_3330|Compound:9141424|FD-Score:3.80|P-value:3.37E-5

Top fitness defect scores for YKR003W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2594 a-mangostin 6.25 100.0 µM 3.45E-11 9 2.11E-10
2 SGTC_2932 9016711 5.61 44.5 µM 2.20E-9 16 9.86E-9
3 SGTC_3305 9127027 5.19 53.8 µM 2.91E-8 6 1.08E-7
4 SGTC_828 2074-0507 4.90 139.0 µM 1.44E-7 RPP1 & pyrimidine depletion 10 4.73E-7
5 SGTC_1118 chelidonine 4.85 707.5 nM 1.90E-7 8 6.12E-7
6 SGTC_2930 9013230 4.57 14.9 µM 8.31E-7 heme biosynthesis & mitochondrial translocase 19 2.39E-6
7 SGTC_1032 k061-1018 4.55 98.7 µM 9.60E-7 11 2.74E-6
8 SGTC_1011 4112-3381 4.26 17.4 µM 4.01E-6 32 1.03E-5
9 SGTC_1554 st069299 4.18 38.7 µM 5.76E-6 17 1.44E-5
10 SGTC_3132 9126706 4.16 49.5 µM 6.54E-6 17 1.61E-5
11 SGTC_2030 5175110 4.11 200.0 µM 8.28E-6 Golgi 24 2.01E-5
12 SGTC_925 2064-1272 4.05 59.6 µM 1.05E-5 23 2.51E-5
13 SGTC_2966 9084041 3.99 48.0 µM 1.43E-5 31 3.32E-5
14 SGTC_1302 1159-0335 3.95 255.0 µM 1.67E-5 13 3.84E-5
15 SGTC_3330 9141424 3.80 71.4 µM 3.37E-5 amide catabolism 23 7.34E-5
16 SGTC_1602 st001878 3.77 87.6 µM 3.79E-5 21 8.18E-5
17 SGTC_550 4123-0385 3.77 25.7 µM 3.81E-5 TSC3-RPN4 31 8.23E-5
18 SGTC_2676 harmalol 3.63 100.0 µM 6.94E-5 mitochondrial processes 16 1.43E-4
19 SGTC_1247 0448-0043 3.62 5.8 µM 7.19E-5 ERG2 63 1.48E-4
20 SGTC_1043 0916-0009 3.50 19.3 µM 1.16E-4 36 2.31E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.119 5.14E-12 YPL251W_d YPL251W_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
0.117 1.09E-11 YDR415C_p YDR415C_p hom Putative aminopeptidase
0.117 1.27E-11 YML125C PGA3 het Putative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of lifespan; required for maturation of Gas1p and Pho8p, proposed to be involved in protein trafficking; PGA3 has a paralog, AIM33, that arose from the whole genome duplication
0.103 2.17E-9 YOR318C_d YOR318C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo
0.100 6.25E-9 YJL055W_p YJL055W_p hom Putative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU
0.096 2.33E-8 YDR206W EBS1 hom Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors
0.096 2.48E-8 YKR028W SAP190 hom Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication
0.090 1.63E-7 YIR032C DAL3 hom Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression
0.084 9.50E-7 YPL252C YAH1 het Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
0.084 1.04E-6 YDR392W SPT3 hom Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
0.084 1.20E-6 YOR171C LCB4 hom Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes
0.083 1.43E-6 YDR245W MNN10 hom Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family
0.083 1.47E-6 YDR431W_d YDR431W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.079 4.06E-6 YGR087C PDC6 hom Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation
0.076 9.64E-6 YEL013W VAC8 hom Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions