Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
Profile for YKR011C / YKR011C

Click on Significant Values for Screen Details

Top fitness defect scores for YKR011C deletion by condition

RankScreen IDConditionFD ScoreConc
1 SGTC_1071 trifluoperazine 7.44 6.3 µM 1.59E-15 2 5.12E-14
2 SGTC_632 0781-2353 7.36 4.7 µM 3.00E-15 Golgi 5 8.97E-14
3 SGTC_2099 5377090 5.64 177.4 µM 1.11E-9 4 8.26E-9
4 SGTC_833 3852-0053 5.48 558.0 µM 3.13E-9 3 2.08E-8
5 SGTC_2723 salbutamol 5.37 83.6 µM 6.25E-9 1 3.85E-8
6 SGTC_3306 9127676 5.33 59.8 µM 8.26E-9 6 4.94E-8
7 SGTC_2969 9091408 5.17 28.7 µM 2.23E-8 8 1.20E-7
8 SGTC_2326 6164455 4.80 177.6 µM 1.83E-7 mitochondrial processes 9 7.85E-7
9 SGTC_2695 st077686 4.64 52.0 µM 4.57E-7 21 1.78E-6
10 SGTC_1529 apigenin 4.57 74.0 µM 6.38E-7 19 2.40E-6
11 SGTC_2299 7976368 4.51 114.9 µM 9.09E-7 19 3.29E-6
12 SGTC_2403 6032726 4.43 181.2 µM 1.38E-6 27 4.77E-6
13 SGTC_2319 6033012 4.24 152.6 µM 3.66E-6 tubulin folding & SWR complex 28 1.14E-5
14 SGTC_1608 st001473 4.18 40.5 µM 4.81E-6 30 1.46E-5
15 SGTC_6 0844-0013 4.03 19.8 µM 9.71E-6 79 2.73E-5
16 SGTC_2947 9072639 3.96 7.7 µM 1.36E-5 tubulin folding & SWR complex 42 3.68E-5
17 SGTC_2323 6148992 3.92 148.3 µM 1.63E-5 37 4.35E-5
18 SGTC_1853 st056710 3.89 91.2 µM 1.90E-5 amide catabolism 26 4.98E-5
19 SGTC_2848 9016370 3.53 26.0 µM 9.37E-5 40 2.08E-4
20 SGTC_1951 sophoraflavanone G 3.46 9.2 µM 1.25E-4 RNA processing & uracil transport 50 2.70E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.149 3.28E-18 YLR398C SKI2 hom Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay
0.129 5.56E-14 YPL092W SSU1 hom Plasma membrane sulfite pump involved in sulfite metabolism and required for efficient sulfite efflux; major facilitator superfamily protein
0.111 1.23E-10 YEL023C_p YEL023C_p hom Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene
0.111 1.26E-10 YLR263W RED1 hom Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p
0.107 4.30E-10 YLL062C MHT1 hom S-methylmethionine-homocysteine methyltransferase, functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio
0.095 3.42E-8 YKL042W SPC42 het Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
0.094 5.28E-8 YMR145C NDE1 hom Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain
0.093 7.50E-8 YJR046W TAH11 het DNA replication licensing factor, required for pre-replication complex assembly
0.093 8.22E-8 YJR019C TES1 hom Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids
0.092 9.24E-8 YNL039W BDP1 het Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs
0.086 6.18E-7 YBL028C YBL028C hom Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis
0.081 2.68E-6 YDR051C DET1 hom Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel
0.080 3.21E-6 YKR009C FOX2 hom Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities
0.079 5.28E-6 YEL047C FRD1 hom Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication
0.077 7.92E-6 YMR092C AIP1 hom Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress