HIPHOP chemogenomics database

YKR014C / YPT52

Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain

fixedexpanded
Profile for YKR014C / YPT52

Click on Significant Values for Screen Details

Top fitness defect scores for YKR014C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_805	3852-0218	11.50	479.0 µM	1.07E-28	ERAD & cell cycle	3	5.13E-31
2	SGTC_1715	st032160	9.46	10.2 µM	5.67E-20		7	1.55E-21
3	SGTC_1161	k007-0610	6.84	110.0 µM	2.59E-11	ERAD & cell cycle	42	3.94E-12
4	SGTC_373	0844-0104	6.78	25.9 µM	3.86E-11	ERAD & cell cycle	18	6.08E-12
5	SGTC_352	k048-0007	6.69	5.1 µM	6.91E-11	ERAD & cell cycle	24	1.14E-11
6	SGTC_733	4069-0007	6.57	56.1 µM	1.46E-10	ERAD & cell cycle	34	2.57E-11
7	SGTC_568	1193-0097	5.89	7.5 µM	7.66E-9	ERAD & cell cycle	62	1.89E-9
8	SGTC_384	k007-0144	5.60	5.2 µM	3.79E-8	ERAD & cell cycle	30	1.07E-8
9	SGTC_2291	7917025	5.42	104.3 µM	9.73E-8	iron homeostasis	27	2.97E-8
10	SGTC_103	0844-0104	5.42	51.8 µM	9.82E-8	ERAD & cell cycle	89	3.00E-8
11	SGTC_912	3474-0017	5.33	2.2 µM	1.54E-7		8	4.90E-8
12	SGTC_2322	7413319	5.04	38.2 µM	6.58E-7	ERAD & cell cycle	94	2.36E-7
13	SGTC_3197	9113331	4.87	49.5 µM	1.47E-6		19	5.64E-7
14	SGTC_2812	7970281	4.77	58.4 µM	2.30E-6		8	9.15E-7
15	SGTC_2721	ifenprodil	4.62	61.4 µM	4.57E-6	plasma membrane duress	10	1.92E-6
16	SGTC_2297	7971671	4.15	152.3 µM	3.34E-5	RNA processing & uracil transport	19	1.65E-5
17	SGTC_1716	st033144	4.08	30.6 µM	4.36E-5		20	2.21E-5
18	SGTC_2050	5263056	3.99	171.0 µM	6.40E-5	fatty acid desaturase (OLE1)	40	3.34E-5
19	SGTC_2394	5221648	3.89	200.0 µM	9.30E-5		23	5.01E-5
20	SGTC_2440	5628896	3.87	157.8 µM	9.97E-5		28	5.41E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.428	1.08E-149	YIL030C	SSM4	hom	Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation
0.412	4.61E-138	YOL002C	IZH2	hom	Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc
0.388	3.41E-121	YMR029C	FAR8	hom	Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
0.383	1.77E-117	YDR186C	YDR186C	hom	Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.369	8.42E-109	YER084W_p	YER084W_p	hom	Protein of unknown function; expressed at both mRNA and protein levels
0.359	2.25E-102	YDR199W_d	YDR199W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity
0.311	5.91E-76	YMR052C-A_d	YMR052C-A_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.305	5.97E-73	YDL100C	GET3	hom	Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress
0.290	7.34E-66	YMR022W	UBC7	hom	Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
0.275	1.87E-59	YMR052W	FAR3	hom	Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
0.253	3.12E-50	YLR065C	ENV10	hom	Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene
0.252	1.54E-49	YFR008W	FAR7	hom	Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress
0.252	1.58E-49	YOR246C	ENV9	hom	Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
0.244	1.20E-46	YMR067C	UBX4	hom	UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress
0.241	1.67E-45	YGL055W	OLE1	het	Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria