YKR017C / HEL1

RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU)

Zygosity: Homozygous strain
Profile for YKR017C / HEL1

Click on Significant Values for Screen Details

Top fitness defect scores for YKR017C deletion by condition

RankScreen IDConditionFD ScoreConc
1 SGTC_170 4197-0004 6.46 226.0 µM 3.91E-11 2 5.34E-11
2 SGTC_162 k030-0015 5.54 37.8 µM 1.27E-8 iron homeostasis 3 1.56E-8
3 SGTC_533 1222-0222 5.08 6.7 µM 1.62E-7 PDR1 9 1.90E-7
4 SGTC_1472 k841-0093 4.64 193.0 µM 1.54E-6 7 1.74E-6
5 SGTC_3038 9091290 4.45 49.5 µM 3.77E-6 8 4.20E-6
6 SGTC_1604 st002052 4.32 72.4 µM 6.93E-6 TSC3-RPN4 19 7.64E-6
7 SGTC_1951 sophoraflavanone G 3.95 9.2 µM 3.70E-5 RNA processing & uracil transport 22 3.98E-5
8 SGTC_326 k292-0812 3.92 17.5 µM 4.07E-5 7 4.36E-5
9 SGTC_2162 5663267 3.84 200.0 µM 5.77E-5 15 6.15E-5
10 SGTC_3303 9125618 3.81 40.6 µM 6.60E-5 51 7.02E-5
11 SGTC_1571 st070080 3.78 73.4 µM 7.26E-5 22 7.72E-5
12 SGTC_2141 5621638 3.68 7.7 µM 1.09E-4 tubulin folding & SWR complex 34 1.15E-4
13 SGTC_2996 9066192 3.67 71.4 µM 1.15E-4 43 1.21E-4
14 SGTC_2300 7976697 3.60 71.6 µM 1.51E-4 ERG2 45 1.58E-4
15 SGTC_334 1502-1024 3.57 68.5 µM 1.68E-4 20 1.76E-4
16 SGTC_1014 4130-3005 3.57 69.3 µM 1.70E-4 32 1.78E-4
17 SGTC_635 k038-0028 3.54 24.7 µM 1.91E-4 ubiquinone biosynthesis & proteasome 59 2.00E-4
18 SGTC_1108 betulinic acid 3.53 729.1 nM 2.02E-4 24 2.11E-4
19 SGTC_3290 9121031 3.50 71.4 µM 2.24E-4 23 2.35E-4
20 SGTC_1940 st075990 3.47 51.1 µM 2.49E-4 60S ribosome export 34 2.60E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.150 2.49E-18 YGL152C_d YGL152C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF PEX14/YGL153W
0.135 4.77E-15 YOL138C RTC1 hom Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif
0.118 7.02E-12 YGR008C STF2 hom Protein involved in resistance to dessication stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to the F0 sector of mitochondrial F1F0 ATPase in vitro and is proposed to modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress
0.113 6.40E-11 YFR026C ULI1 hom Putative protein of unknown function involved in and induced by the endoplasmic reticulum unfolded protein response
0.105 9.74E-10 YJR108W ABM1 hom Protein of unknown function, required for normal microtubule organization
0.094 4.47E-8 YKL161C KDX1 hom Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p
0.089 2.52E-7 YGL111W NSA1 het Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis
0.088 3.65E-7 YDR377W ATP17 hom Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis
0.087 4.02E-7 YML047C PRM6 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; regulated by Ste12p during mating; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family
0.086 5.33E-7 YNL113W RPC19 het RNA polymerase subunit AC19, common to RNA polymerases I and III
0.082 2.27E-6 YMR134W ERG29 het Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes
0.077 8.28E-6 YGR131W FHN1 hom Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain
0.077 8.56E-6 YOR053W_d YOR053W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C
0.077 9.03E-6 YKL102C_d YKL102C_d hom Dubious open reading frame unlikely to encode a functional protein; deletion confers sensitivity to citric acid; predicted protein would include a thiol-disulfide oxidoreductase active site
0.076 9.15E-6 YPR114W_p YPR114W_p hom Putative protein of unknown function