HIPHOP chemogenomics database

YLL021W / SPA2

Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate

Zygosity: Homozygous strain

fixedexpanded
Profile for YLL021W / SPA2

Click on Significant Values for Screen Details

Top fitness defect scores for YLL021W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_138	0850-0259	16.40	40.9 µM	5.10E-51	cell wall	14	4.42E-61
2	SGTC_354	2783-3500	7.69	4.9 µM	9.95E-13	cell wall	11	7.23E-15
3	SGTC_2662	daunorubicin	7.40	22.4 µM	6.32E-12	anthracycline transcription coupled DNA repair	3	6.67E-14
4	SGTC_1220	1838-0075	6.99	193.0 µM	7.84E-11		2	1.37E-12
5	SGTC_2619	obliquin	6.61	87.9 µM	7.00E-10	cell wall	65	1.89E-11
6	SGTC_2584	androsterone	6.45	48.7 µM	1.73E-9	cell wall	49	5.59E-11
7	SGTC_3304	9125678	6.38	46.1 µM	2.51E-9		11	8.74E-11
8	SGTC_304	1310-0298	5.94	97.0 µM	2.57E-8	cell wall	21	1.42E-9
9	SGTC_1282	0886-0010	5.71	32.4 µM	8.28E-8	endomembrane recycling	11	5.74E-9
10	SGTC_221	trifluoperazine	5.49	10.3 µM	2.33E-7	NEO1-PIK1	144	1.98E-8
11	SGTC_916	4466-0038	5.44	42.0 nM	3.00E-7	cell wall	32	2.67E-8
12	SGTC_453	enantio-paf c16	5.21	10.0 µM	8.52E-7		80	9.31E-8
13	SGTC_1340	1486-1359	5.08	257.0 µM	1.51E-6		6	1.84E-7
14	SGTC_2612	dictamnine	5.04	15.5 µM	1.82E-6	RNA pol III & RNase P/MRP	23	2.30E-7
15	SGTC_2756	carbadox	4.95	75.1 µM	2.67E-6		35	3.63E-7
16	SGTC_3311	9131146	4.92	2.9 µM	3.03E-6	cell wall	87	4.22E-7
17	SGTC_2602	kawain	4.69	100.0 µM	8.22E-6		11	1.39E-6
18	SGTC_1456	k015-0012	4.54	13.0 µM	1.48E-5		21	2.79E-6
19	SGTC_2289	7946404	4.48	165.1 µM	1.91E-5		75	3.79E-6
20	SGTC_2608	thymoquinone	4.39	42.7 µM	2.70E-5		9	5.70E-6

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.385	5.47E-119	YJL159W	HSP150	hom	O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.366	4.88E-107	YDL203C	ACK1	hom	Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.363	6.13E-105	YJR142W_p	YJR142W_p	hom	Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.360	2.30E-103	YJL099W	CHS6	hom	Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.358	6.57E-102	YMR124W_p	YMR124W_p	hom	Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.355	5.32E-100	YML117W	NAB6	hom	Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP
0.349	5.36E-97	YJR054W	KCH1	hom	Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.341	7.65E-92	YBR023C	CHS3	hom	Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
0.319	2.14E-80	YPL066W	RGL1	hom	Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
0.317	4.50E-79	YLL032C	YLL032C	hom	Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.301	2.66E-71	YOL003C	PFA4	hom	Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.296	6.39E-69	YDR351W	SBE2	hom	Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth
0.293	2.78E-67	YJL073W	JEM1	hom	DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2
0.291	1.84E-66	YPL115C	BEM3	hom	Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
0.291	2.28E-66	YBL006C	LDB7	hom	Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions