YLR103C / CDC45

DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR103C / CDC45

Click on Significant Values for Screen Details ID:SGTC_55|Compound:0767-0285|FD-Score:4.96|P-value:1.69E-8 ID:SGTC_188|Compound:3229-0966|FD-Score:-3.14|P-value:2.16E-4 ID:SGTC_260|Compound:nsc-65238|FD-Score:-2.86|P-value:6.68E-4 ID:SGTC_316|Compound:k060-0003|FD-Score:2.91|P-value:6.06E-4 ID:SGTC_441|Compound:propafenone|FD-Score:3.90|P-value:6.97E-6 ID:SGTC_600|Compound:1495-0418|FD-Score:2.86|P-value:7.37E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:4.54|P-value:2.18E-7 ID:SGTC_622|Compound:1348-1566|FD-Score:3.34|P-value:1.01E-4 ID:SGTC_631|Compound:k072-0230|FD-Score:3.28|P-value:1.30E-4 ID:SGTC_789|Compound:1309-1135|FD-Score:2.83|P-value:8.34E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:3.00|P-value:4.31E-4 ID:SGTC_958|Compound:1222-0007|FD-Score:2.85|P-value:7.56E-4 ID:SGTC_963|Compound:1269-2853|FD-Score:-2.83|P-value:7.54E-4 ID:SGTC_1045|Compound:1348-1485|FD-Score:-3.45|P-value:5.49E-5 ID:SGTC_1120|Compound:ficusin|FD-Score:-3.72|P-value:1.58E-5 ID:SGTC_1181|Compound:1222-0449|FD-Score:2.79|P-value:9.72E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:4.96|P-value:1.70E-8 ID:SGTC_1322|Compound:1348-1070|FD-Score:2.85|P-value:7.81E-4 ID:SGTC_1349|Compound:flufenamic acid|FD-Score:3.46|P-value:5.99E-5 ID:SGTC_1356|Compound:1502-0781|FD-Score:3.76|P-value:1.42E-5 ID:SGTC_1363|Compound:1636-0063|FD-Score:-3.50|P-value:4.51E-5 ID:SGTC_1365|Compound:1672-3266|FD-Score:-3.37|P-value:7.86E-5 ID:SGTC_1366|Compound:1683-0113|FD-Score:-5.79|P-value:4.54E-11 ID:SGTC_1681|Compound:tripelennamine|FD-Score:3.65|P-value:2.47E-5 ID:SGTC_1787|Compound:fluconazole|FD-Score:2.93|P-value:5.71E-4 ID:SGTC_1850|Compound:st056238|FD-Score:-3.90|P-value:6.23E-6 ID:SGTC_2427|Compound:5545055|FD-Score:-2.76|P-value:9.91E-4 ID:SGTC_2468|Compound:5987765|FD-Score:-3.01|P-value:3.67E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:5.43|P-value:7.34E-10 ID:SGTC_2619|Compound:obliquin|FD-Score:3.98|P-value:4.86E-6 ID:SGTC_2691|Compound:st077356|FD-Score:2.86|P-value:7.29E-4 ID:SGTC_2718|Compound:lomofungin|FD-Score:3.08|P-value:3.11E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:2.82|P-value:8.75E-4 ID:SGTC_2765|Compound:myclobutanil|FD-Score:2.87|P-value:7.17E-4 ID:SGTC_2954|Compound:9074818|FD-Score:2.84|P-value:7.98E-4 ID:SGTC_3094|Compound:9117742|FD-Score:3.63|P-value:2.72E-5 ID:SGTC_3097|Compound:9117246|FD-Score:3.13|P-value:2.55E-4 ID:SGTC_3264|Compound:9137442|FD-Score:-3.71|P-value:1.62E-5 ID:SGTC_3281|Compound:9140613|FD-Score:4.45|P-value:3.64E-7 ID:SGTC_55|Compound:0767-0285|FD-Score:4.96|P-value:1.69E-8 ID:SGTC_188|Compound:3229-0966|FD-Score:-3.14|P-value:2.16E-4 ID:SGTC_260|Compound:nsc-65238|FD-Score:-2.86|P-value:6.68E-4 ID:SGTC_316|Compound:k060-0003|FD-Score:2.91|P-value:6.06E-4 ID:SGTC_441|Compound:propafenone|FD-Score:3.90|P-value:6.97E-6 ID:SGTC_600|Compound:1495-0418|FD-Score:2.86|P-value:7.37E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:4.54|P-value:2.18E-7 ID:SGTC_622|Compound:1348-1566|FD-Score:3.34|P-value:1.01E-4 ID:SGTC_631|Compound:k072-0230|FD-Score:3.28|P-value:1.30E-4 ID:SGTC_789|Compound:1309-1135|FD-Score:2.83|P-value:8.34E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:3.00|P-value:4.31E-4 ID:SGTC_958|Compound:1222-0007|FD-Score:2.85|P-value:7.56E-4 ID:SGTC_963|Compound:1269-2853|FD-Score:-2.83|P-value:7.54E-4 ID:SGTC_1045|Compound:1348-1485|FD-Score:-3.45|P-value:5.49E-5 ID:SGTC_1120|Compound:ficusin|FD-Score:-3.72|P-value:1.58E-5 ID:SGTC_1181|Compound:1222-0449|FD-Score:2.79|P-value:9.72E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:4.96|P-value:1.70E-8 ID:SGTC_1322|Compound:1348-1070|FD-Score:2.85|P-value:7.81E-4 ID:SGTC_1349|Compound:flufenamic acid|FD-Score:3.46|P-value:5.99E-5 ID:SGTC_1356|Compound:1502-0781|FD-Score:3.76|P-value:1.42E-5 ID:SGTC_1363|Compound:1636-0063|FD-Score:-3.50|P-value:4.51E-5 ID:SGTC_1365|Compound:1672-3266|FD-Score:-3.37|P-value:7.86E-5 ID:SGTC_1366|Compound:1683-0113|FD-Score:-5.79|P-value:4.54E-11 ID:SGTC_1681|Compound:tripelennamine|FD-Score:3.65|P-value:2.47E-5 ID:SGTC_1787|Compound:fluconazole|FD-Score:2.93|P-value:5.71E-4 ID:SGTC_1850|Compound:st056238|FD-Score:-3.90|P-value:6.23E-6 ID:SGTC_2427|Compound:5545055|FD-Score:-2.76|P-value:9.91E-4 ID:SGTC_2468|Compound:5987765|FD-Score:-3.01|P-value:3.67E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:5.43|P-value:7.34E-10 ID:SGTC_2619|Compound:obliquin|FD-Score:3.98|P-value:4.86E-6 ID:SGTC_2691|Compound:st077356|FD-Score:2.86|P-value:7.29E-4 ID:SGTC_2718|Compound:lomofungin|FD-Score:3.08|P-value:3.11E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:2.82|P-value:8.75E-4 ID:SGTC_2765|Compound:myclobutanil|FD-Score:2.87|P-value:7.17E-4 ID:SGTC_2954|Compound:9074818|FD-Score:2.84|P-value:7.98E-4 ID:SGTC_3094|Compound:9117742|FD-Score:3.63|P-value:2.72E-5 ID:SGTC_3097|Compound:9117246|FD-Score:3.13|P-value:2.55E-4 ID:SGTC_3264|Compound:9137442|FD-Score:-3.71|P-value:1.62E-5 ID:SGTC_3281|Compound:9140613|FD-Score:4.45|P-value:3.64E-7

Top fitness defect scores for YLR103C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2544 sphondin 5.43 68.5 µM 7.34E-10 RNA pol III & RNase P/MRP 22 2.81E-8 0.10
2 SGTC_55 0767-0285 4.96 6.6 µM 1.69E-8 4 3.57E-7 0.57
3 SGTC_1310 1188-0297 4.96 994.0 nM 1.70E-8 azole & statin 9 3.58E-7 0.46
4 SGTC_621 3584-0052 4.54 32.1 µM 2.18E-7 4 2.83E-6 0.86
5 SGTC_3281 9140613 4.45 49.5 µM 3.64E-7 RPP1 & pyrimidine depletion 4 4.29E-6 0.84
6 SGTC_2619 obliquin 3.98 87.9 µM 4.86E-6 cell wall 30 3.52E-5 0.11
7 SGTC_441 propafenone 3.90 146.0 µM 6.97E-6 5 4.72E-5 0.46
8 SGTC_1356 1502-0781 3.76 85.8 µM 1.42E-5 fatty acid desaturase (OLE1) 6 8.44E-5 0.08
9 SGTC_1681 tripelennamine 3.65 100.0 µM 2.47E-5 10 1.32E-4 0.00
10 SGTC_3094 9117742 3.63 49.5 µM 2.72E-5 6 1.43E-4 0.02
11 SGTC_1349 flufenamic acid 3.46 69.0 µM 5.99E-5 4 2.72E-4 0.06
12 SGTC_622 1348-1566 3.34 26.3 µM 1.01E-4 8 4.17E-4 0.07
13 SGTC_631 k072-0230 3.28 41.5 µM 1.30E-4 60S ribosome export 11 5.11E-4 0.09
14 SGTC_3097 9117246 3.13 49.5 µM 2.55E-4 8 8.87E-4 0.07
15 SGTC_2718 lomofungin 3.08 53.0 µM 3.11E-4 mitochondrial processes 18 0.00104 0.03
16 SGTC_925 2064-1272 3.00 59.6 µM 4.31E-4 17 0.00136 9.87E-4
17 SGTC_1787 fluconazole 2.93 80.0 µM 5.71E-4 iron homeostasis 24 0.00171 0.01
18 SGTC_316 k060-0003 2.91 58.6 µM 6.06E-4 17 0.00180 0.02
19 SGTC_2765 myclobutanil 2.87 7.7 µM 7.17E-4 azole & statin 29 0.00207 0.00
20 SGTC_2691 st077356 2.86 60.2 µM 7.29E-4 18 0.00209 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.153 5.17E-19 YJL159W HSP150 hom O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.111 1.31E-10 YPL229W_p YPL229W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication
0.100 7.04E-9 YOR116C RPO31 het RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
0.097 1.79E-8 YGL033W HOP2 hom Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair
0.096 2.47E-8 YKL026C GPX1 hom Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress
0.096 2.66E-8 YOR135C_d IRC14_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci
0.096 2.89E-8 YBR154C RPB5 het RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
0.094 4.19E-8 YNL243W SLA2 hom Adaptor protein that links actin to clathrin and endocytosis; involved in membrane cytoskeleton assembly and cell polarization; present in the actin cortical patch of the emerging bud tip; dimer in vivo
0.092 8.24E-8 YOR176W HEM15 het Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway
0.090 1.53E-7 YBL099W ATP1 hom Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated
0.087 5.01E-7 YIR014W_p YIR014W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; YIR014W is a non-essential gene
0.082 1.77E-6 YLR119W SRN2 hom Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus
0.081 2.42E-6 YGR203W YCH1 hom Phosphatase with sequence similarity to Cdc25p, Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
0.080 3.14E-6 YKR035C_d OPI8_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A
0.078 5.85E-6 YNL248C RPA49 hom RNA polymerase I subunit A49