Putative glutamine amidotransferase; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress

Zygosity: Homozygous strain
Profile for YLR126C / YLR126C

Click on Significant Values for Screen Details

Top fitness defect scores for YLR126C deletion by condition

RankScreen IDConditionFD ScoreConc
1 SGTC_953 1188-0045 5.24 9.7 µM 1.94E-8 60S ribosome export 5 8.24E-8
2 SGTC_1710 st031758 5.17 61.5 µM 2.87E-8 5 1.17E-7
3 SGTC_2417 cerulenin 4.67 830.0 nM 4.78E-7 16 1.52E-6
4 SGTC_689 0833-0039 4.55 98.0 µM 9.05E-7 ergosterol depletion effects on membrane 24 2.72E-6
5 SGTC_2984 9022109 4.26 39.0 µM 3.96E-6 2 1.05E-5
6 SGTC_425 3825-7017 3.97 13.1 µM 1.52E-5 21 3.56E-5
7 SGTC_1949 st076024 3.83 69.1 µM 2.89E-5 27 6.40E-5
8 SGTC_158 k038-0087 3.72 26.4 µM 4.77E-5 ubiquinone biosynthesis & proteasome 158 1.01E-4
9 SGTC_2987 9018338 3.66 71.4 µM 5.98E-5 RPP1 & pyrimidine depletion 30 1.24E-4
10 SGTC_429 1112-0049 3.63 25.5 µM 6.83E-5 DNA damage response 46 1.40E-4
11 SGTC_64 000l-0018 3.54 272.1 µM 1.02E-4 ubiquinone biosynthesis & proteasome 176 2.02E-4
12 SGTC_2495 cafestol 3.54 54.5 µM 1.02E-4 Golgi 36 2.03E-4
13 SGTC_2484 5809818 3.53 12.0 µM 1.03E-4 24 2.04E-4
14 SGTC_2100 5377183 3.47 190.0 µM 1.34E-4 60S ribosome export 21 2.60E-4
15 SGTC_3333 9141820 3.46 12.1 µM 1.42E-4 23 2.73E-4
16 SGTC_2486 5283202 3.45 22.4 µM 1.43E-4 55 2.75E-4
17 SGTC_2179 6298986 3.31 200.0 µM 2.51E-4 heme requiring 33 4.59E-4
18 SGTC_2856 9022925 3.18 19.5 µM 4.25E-4 56 7.44E-4
19 SGTC_2492 derrusnin 3.13 100.0 µM 5.16E-4 19 8.88E-4
20 SGTC_2062 5245942 3.07 200.0 µM 6.22E-4 53 0.00105

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.134 6.57E-15 YLR201C COQ9 hom Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes
0.124 6.38E-13 YNL071W LAT1 hom Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA
0.115 2.07E-11 YGL119W COQ8 hom Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication
0.115 2.93E-11 YNR042W_d YNR042W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.112 6.82E-11 YPR064W_d YPR064W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.112 7.39E-11 YDR204W COQ4 hom Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex
0.112 8.07E-11 YER019C-A SBH2 hom Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p
0.111 1.27E-10 YGR255C COQ6 hom Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS)
0.110 1.46E-10 YPL190C NAB3 het RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing
0.110 1.56E-10 YER041W YEN1 hom Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p
0.107 5.09E-10 YNR041C COQ2 hom Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis
0.106 7.41E-10 YER094C PUP3 het Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
0.105 1.18E-9 YBL041W PRE7 het Beta 6 subunit of the 20S proteasome
0.101 5.13E-9 YML014W TRM9 hom tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses
0.100 7.51E-9 YMR110C HFD1 hom Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder