YLR146C / SPE4

Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR146C / SPE4

Click on Significant Values for Screen Details ID:SGTC_122|Compound:0236-0016|FD-Score:3.67|P-value:5.05E-5 ID:SGTC_137|Compound:0438-0332|FD-Score:3.29|P-value:2.49E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:3.01|P-value:7.08E-4 ID:SGTC_338|Compound:4055-0136|FD-Score:4.11|P-value:6.79E-6 ID:SGTC_412|Compound:gemfibrozil|FD-Score:3.12|P-value:4.76E-4 ID:SGTC_577|Compound:r071-0003|FD-Score:3.31|P-value:2.31E-4 ID:SGTC_709|Compound:3807-1509|FD-Score:2.97|P-value:8.22E-4 ID:SGTC_866|Compound:0495-0101|FD-Score:-3.19|P-value:3.58E-4 ID:SGTC_1140|Compound:3229-1848|FD-Score:-3.54|P-value:8.87E-5 ID:SGTC_1259|Compound:0671-0132|FD-Score:-3.71|P-value:4.13E-5 ID:SGTC_1329|Compound:1447-1697|FD-Score:3.05|P-value:6.19E-4 ID:SGTC_1350|Compound:1495-0454|FD-Score:3.01|P-value:7.16E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:3.23|P-value:3.10E-4 ID:SGTC_1485|Compound:3909-9022|FD-Score:3.39|P-value:1.68E-4 ID:SGTC_1505|Compound:k297-0037|FD-Score:-3.99|P-value:1.16E-5 ID:SGTC_1568|Compound:digitonin|FD-Score:4.37|P-value:1.88E-6 ID:SGTC_1569|Compound:carminic acid|FD-Score:3.73|P-value:3.86E-5 ID:SGTC_1571|Compound:st070080|FD-Score:-3.66|P-value:5.22E-5 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:3.34|P-value:2.02E-4 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:6.43|P-value:4.70E-12 ID:SGTC_1805|Compound:st050013|FD-Score:5.21|P-value:1.75E-8 ID:SGTC_1828|Compound:st055811|FD-Score:3.11|P-value:5.04E-4 ID:SGTC_1831|Compound:st055328|FD-Score:-4.30|P-value:2.50E-6 ID:SGTC_1832|Compound:st054113|FD-Score:3.02|P-value:6.82E-4 ID:SGTC_1836|Compound:st055642|FD-Score:3.18|P-value:3.76E-4 ID:SGTC_1885|Compound:st057546|FD-Score:2.95|P-value:8.83E-4 ID:SGTC_2018|Compound:5133068|FD-Score:-8.52|P-value:8.57E-20 ID:SGTC_2019|Compound:4016004|FD-Score:-6.88|P-value:1.48E-13 ID:SGTC_2022|Compound:5136654|FD-Score:-3.84|P-value:2.37E-5 ID:SGTC_2024|Compound:5144227|FD-Score:3.67|P-value:4.99E-5 ID:SGTC_2124|Compound:5246320|FD-Score:3.34|P-value:2.04E-4 ID:SGTC_2181|Compound:6316240|FD-Score:-2.97|P-value:8.09E-4 ID:SGTC_2191|Compound:5911152|FD-Score:2.99|P-value:7.66E-4 ID:SGTC_2299|Compound:7976368|FD-Score:4.36|P-value:1.93E-6 ID:SGTC_2303|Compound:7774051|FD-Score:3.31|P-value:2.28E-4 ID:SGTC_2316|Compound:5844804|FD-Score:5.06|P-value:4.29E-8 ID:SGTC_2325|Compound:6158266|FD-Score:-3.42|P-value:1.46E-4 ID:SGTC_2347|Compound:9049783|FD-Score:2.93|P-value:9.51E-4 ID:SGTC_2464|Compound:5717415|FD-Score:-3.26|P-value:2.70E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:-2.94|P-value:9.13E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:3.04|P-value:6.43E-4 ID:SGTC_2806|Compound:7952793|FD-Score:4.03|P-value:1.00E-5 ID:SGTC_2808|Compound:7946468|FD-Score:4.21|P-value:4.18E-6 ID:SGTC_2828|Compound:7998861|FD-Score:2.96|P-value:8.58E-4 ID:SGTC_3095|Compound:9117207|FD-Score:3.07|P-value:5.78E-4 ID:SGTC_3212|Compound:9128307|FD-Score:-3.92|P-value:1.62E-5 ID:SGTC_3337|Compound:9144342|FD-Score:3.55|P-value:8.65E-5 ID:SGTC_3343|Compound:9148379|FD-Score:3.81|P-value:2.73E-5 ID:SGTC_122|Compound:0236-0016|FD-Score:3.67|P-value:5.05E-5 ID:SGTC_137|Compound:0438-0332|FD-Score:3.29|P-value:2.49E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:3.01|P-value:7.08E-4 ID:SGTC_338|Compound:4055-0136|FD-Score:4.11|P-value:6.79E-6 ID:SGTC_412|Compound:gemfibrozil|FD-Score:3.12|P-value:4.76E-4 ID:SGTC_577|Compound:r071-0003|FD-Score:3.31|P-value:2.31E-4 ID:SGTC_709|Compound:3807-1509|FD-Score:2.97|P-value:8.22E-4 ID:SGTC_866|Compound:0495-0101|FD-Score:-3.19|P-value:3.58E-4 ID:SGTC_1140|Compound:3229-1848|FD-Score:-3.54|P-value:8.87E-5 ID:SGTC_1259|Compound:0671-0132|FD-Score:-3.71|P-value:4.13E-5 ID:SGTC_1329|Compound:1447-1697|FD-Score:3.05|P-value:6.19E-4 ID:SGTC_1350|Compound:1495-0454|FD-Score:3.01|P-value:7.16E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:3.23|P-value:3.10E-4 ID:SGTC_1485|Compound:3909-9022|FD-Score:3.39|P-value:1.68E-4 ID:SGTC_1505|Compound:k297-0037|FD-Score:-3.99|P-value:1.16E-5 ID:SGTC_1568|Compound:digitonin|FD-Score:4.37|P-value:1.88E-6 ID:SGTC_1569|Compound:carminic acid|FD-Score:3.73|P-value:3.86E-5 ID:SGTC_1571|Compound:st070080|FD-Score:-3.66|P-value:5.22E-5 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:3.34|P-value:2.02E-4 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:6.43|P-value:4.70E-12 ID:SGTC_1805|Compound:st050013|FD-Score:5.21|P-value:1.75E-8 ID:SGTC_1828|Compound:st055811|FD-Score:3.11|P-value:5.04E-4 ID:SGTC_1831|Compound:st055328|FD-Score:-4.30|P-value:2.50E-6 ID:SGTC_1832|Compound:st054113|FD-Score:3.02|P-value:6.82E-4 ID:SGTC_1836|Compound:st055642|FD-Score:3.18|P-value:3.76E-4 ID:SGTC_1885|Compound:st057546|FD-Score:2.95|P-value:8.83E-4 ID:SGTC_2018|Compound:5133068|FD-Score:-8.52|P-value:8.57E-20 ID:SGTC_2019|Compound:4016004|FD-Score:-6.88|P-value:1.48E-13 ID:SGTC_2022|Compound:5136654|FD-Score:-3.84|P-value:2.37E-5 ID:SGTC_2024|Compound:5144227|FD-Score:3.67|P-value:4.99E-5 ID:SGTC_2124|Compound:5246320|FD-Score:3.34|P-value:2.04E-4 ID:SGTC_2181|Compound:6316240|FD-Score:-2.97|P-value:8.09E-4 ID:SGTC_2191|Compound:5911152|FD-Score:2.99|P-value:7.66E-4 ID:SGTC_2299|Compound:7976368|FD-Score:4.36|P-value:1.93E-6 ID:SGTC_2303|Compound:7774051|FD-Score:3.31|P-value:2.28E-4 ID:SGTC_2316|Compound:5844804|FD-Score:5.06|P-value:4.29E-8 ID:SGTC_2325|Compound:6158266|FD-Score:-3.42|P-value:1.46E-4 ID:SGTC_2347|Compound:9049783|FD-Score:2.93|P-value:9.51E-4 ID:SGTC_2464|Compound:5717415|FD-Score:-3.26|P-value:2.70E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:-2.94|P-value:9.13E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:3.04|P-value:6.43E-4 ID:SGTC_2806|Compound:7952793|FD-Score:4.03|P-value:1.00E-5 ID:SGTC_2808|Compound:7946468|FD-Score:4.21|P-value:4.18E-6 ID:SGTC_2828|Compound:7998861|FD-Score:2.96|P-value:8.58E-4 ID:SGTC_3095|Compound:9117207|FD-Score:3.07|P-value:5.78E-4 ID:SGTC_3212|Compound:9128307|FD-Score:-3.92|P-value:1.62E-5 ID:SGTC_3337|Compound:9144342|FD-Score:3.55|P-value:8.65E-5 ID:SGTC_3343|Compound:9148379|FD-Score:3.81|P-value:2.73E-5

Top fitness defect scores for YLR146C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1575 cyproterone acetate 6.43 48.0 µM 4.70E-12 2 6.19E-11
2 SGTC_1805 st050013 5.21 60.3 µM 1.75E-8 1 9.59E-8
3 SGTC_2316 5844804 5.06 197.4 µM 4.29E-8 8 2.14E-7
4 SGTC_1568 digitonin 4.37 820.0 nM 1.88E-6 sphingolipid biosynthesis & PDR1 24 6.30E-6
5 SGTC_2299 7976368 4.36 114.9 µM 1.93E-6 20 6.47E-6
6 SGTC_2808 7946468 4.21 45.5 µM 4.18E-6 17 1.29E-5
7 SGTC_338 4055-0136 4.11 114.0 µM 6.79E-6 7 1.99E-5
8 SGTC_2806 7952793 4.03 58.4 µM 1.00E-5 18 2.82E-5
9 SGTC_3343 9148379 3.81 19.0 µM 2.73E-5 5 6.93E-5
10 SGTC_1569 carminic acid 3.73 40.6 µM 3.86E-5 23 9.45E-5
11 SGTC_2024 5144227 3.67 134.0 µM 4.99E-5 46 1.19E-4
12 SGTC_122 0236-0016 3.67 402.4 µM 5.05E-5 RNA processing & uracil transport 43 1.20E-4
13 SGTC_3337 9144342 3.55 33.5 µM 8.65E-5 37 1.95E-4
14 SGTC_1485 3909-9022 3.39 254.0 µM 1.68E-4 RPP1 & pyrimidine depletion 21 3.54E-4
15 SGTC_1572 epiandrosterone 3.34 68.9 µM 2.02E-4 46 4.16E-4
16 SGTC_2124 5246320 3.34 200.0 µM 2.04E-4 heme biosynthesis & mitochondrial translocase 41 4.20E-4
17 SGTC_2303 7774051 3.31 200.0 µM 2.28E-4 43 4.64E-4
18 SGTC_577 r071-0003 3.31 38.6 µM 2.31E-4 fatty acid desaturase (OLE1) 93 4.70E-4
19 SGTC_137 0438-0332 3.29 85.4 µM 2.49E-4 ubiquinone biosynthesis & proteasome 163 5.02E-4
20 SGTC_1360 1598-0022 3.23 39.8 µM 3.10E-4 ERG2 68 6.12E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.205 2.53E-33 YNL245C CWC25 het Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p
0.121 1.92E-12 YOR313C SPS4 hom Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage
0.102 3.54E-9 YMR102C_p YMR102C_p hom Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene
0.102 3.69E-9 YHR073W OSH3 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.098 1.18E-8 YGL131C SNT2 hom DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.098 1.32E-8 YGR248W SOL4 hom 6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication
0.096 2.20E-8 YOR329C SCD5 het Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency
0.096 2.61E-8 YJR053W BFA1 hom Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis
0.096 2.84E-8 YDR306C_p YDR306C_p hom F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain
0.095 3.98E-8 YHR014W SPO13 hom Meiosis-specific protein, involved in maintaining sister chromatid cohesion during meiosis I as well as promoting proper attachment of kinetochores to the spindle during meiosis I and meiosis II
0.091 1.11E-7 YJL151C SNA3 hom Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles
0.090 1.54E-7 YMR094W CTF13 het Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis
0.090 2.05E-7 YCR043C_p YCR043C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene
0.088 3.08E-7 YGR047C TFC4 het One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102
0.088 3.56E-7 YBR118W TEF2 hom Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication