Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
Profile for YLR149C / YLR149C

Click on Significant Values for Screen Details

Top fitness defect scores for YLR149C deletion by condition

RankScreen IDConditionFD ScoreConc
1 SGTC_1742 st038235 9.87 28.9 µM 4.30E-21 mitochondrial stress 11 2.90E-23
2 SGTC_236 1509-0011 9.28 5.3 µM 7.40E-19 209 8.69E-21
3 SGTC_275 trichlorophene 8.34 5.7 µM 1.43E-15 mitochondrial stress 67 3.81E-17
4 SGTC_173 1273-0061 8.20 2.7 µM 4.20E-15 mitochondrial stress 61 1.25E-16
5 SGTC_310 1273-0058 7.83 1.1 µM 5.95E-14 mitochondrial stress 97 2.36E-15
6 SGTC_3238 9133470 5.87 49.5 µM 1.40E-8 1 2.13E-9
7 SGTC_2232 chlorophetanol 5.70 41.5 µM 3.53E-8 TRP & mitochondrial translation 47 5.91E-9
8 SGTC_70 0141-0289 5.29 43.3 µM 2.87E-7 mitochondrial stress 103 6.04E-8
9 SGTC_832 4083-0019 5.29 164.0 µM 2.89E-7 translation 32 6.09E-8
10 SGTC_2944 9067346 4.94 25.6 µM 1.54E-6 10 3.88E-7
11 SGTC_1093 fluphenazine 4.59 8.0 µM 7.44E-6 9 2.23E-6
12 SGTC_2595 hexamethylquercetagetin 4.48 100.0 µM 1.19E-5 15 3.75E-6
13 SGTC_2579 2',5'-dihydroxy-4-methoxychalcone 4.29 13.2 µM 2.58E-5 21 8.86E-6
14 SGTC_2598 perillyl alcohol 4.26 100.0 µM 2.97E-5 22 1.03E-5
15 SGTC_2113 5491891 4.23 107.0 µM 3.37E-5 TSC3-RPN4 13 1.19E-5
16 SGTC_3224 9130643 4.19 49.5 µM 3.88E-5 RPP1 & pyrimidine depletion 23 1.39E-5
17 SGTC_2688 kanamycin b 4.14 96.7 µM 4.77E-5 calcium & mitochondrial duress 342 1.75E-5
18 SGTC_3083 9117465 4.12 49.5 µM 5.17E-5 16 1.91E-5
19 SGTC_174 3553-0389 4.06 74.3 µM 6.41E-5 20 2.42E-5
20 SGTC_2750 bromebric acid 4.06 70.2 µM 6.41E-5 26 2.43E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.489 2.60E-201 YER050C RSM18 hom Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein
0.468 1.05E-181 YMR287C DSS1 hom 3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs
0.467 1.55E-181 YMR193W MRPL24 hom Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene
0.458 2.45E-173 YNL177C MRPL22 hom Mitochondrial ribosomal protein of the large subunit
0.456 2.68E-172 YGR215W RSM27 hom Mitochondrial ribosomal protein of the small subunit
0.450 4.91E-167 YPL040C ISM1 hom Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth
0.443 1.56E-161 YMR228W MTF1 hom Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
0.440 2.51E-158 YMR098C ATP25 hom Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene
0.433 3.27E-153 YGR102C GTF1 hom Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria
0.433 4.05E-153 YKL170W MRPL38 hom Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress
0.429 2.93E-150 YDR298C ATP5 hom Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated
0.422 2.54E-145 YER087W AIM10 hom Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss
0.422 3.49E-145 YDR194C MSS116 hom DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing
0.420 1.29E-143 YLR069C MEF1 hom Mitochondrial elongation factor involved in translational elongation
0.418 2.47E-142 YPR166C MRP2 hom Mitochondrial ribosomal protein of the small subunit