YLR154C / RNH203

Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS3 that causes Aicardi-Goutieres syndrome

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR154C / RNH203

Click on Significant Values for Screen Details ID:SGTC_215|Compound:1287-0124|FD-Score:3.30|P-value:1.95E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:3.11|P-value:4.24E-4 ID:SGTC_374|Compound:0880-0231|FD-Score:4.92|P-value:6.52E-8 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:3.83|P-value:1.95E-5 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:3.64|P-value:4.61E-5 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.54|P-value:7.17E-5 ID:SGTC_782|Compound:0083-0095|FD-Score:-4.05|P-value:8.09E-6 ID:SGTC_785|Compound:1270-0111|FD-Score:-4.56|P-value:5.99E-7 ID:SGTC_812|Compound:4251-0442|FD-Score:2.94|P-value:7.81E-4 ID:SGTC_868|Compound:0527-0199|FD-Score:2.96|P-value:7.29E-4 ID:SGTC_898|Compound:1000-0849|FD-Score:3.70|P-value:3.61E-5 ID:SGTC_900|Compound:1112-0045|FD-Score:-3.81|P-value:2.55E-5 ID:SGTC_956|Compound:1185-0240|FD-Score:-3.09|P-value:5.03E-4 ID:SGTC_958|Compound:1222-0007|FD-Score:3.13|P-value:3.81E-4 ID:SGTC_1294|Compound:1000-1494|FD-Score:3.15|P-value:3.56E-4 ID:SGTC_1303|Compound:1160-0052|FD-Score:-4.33|P-value:2.01E-6 ID:SGTC_1311|Compound:1190-0148|FD-Score:3.23|P-value:2.62E-4 ID:SGTC_1312|Compound:1218-2183|FD-Score:4.01|P-value:8.47E-6 ID:SGTC_1692|Compound:fluoroquinolonic acid|FD-Score:4.26|P-value:2.47E-6 ID:SGTC_1927|Compound:st060202|FD-Score:3.11|P-value:4.17E-4 ID:SGTC_1948|Compound:st076329|FD-Score:2.88|P-value:9.75E-4 ID:SGTC_2160|Compound:5660466|FD-Score:3.20|P-value:3.01E-4 ID:SGTC_2238|Compound:6671620|FD-Score:-3.43|P-value:1.31E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-4.30|P-value:2.36E-6 ID:SGTC_2394|Compound:5221648|FD-Score:3.48|P-value:9.38E-5 ID:SGTC_2429|Compound:5902844|FD-Score:3.64|P-value:4.64E-5 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:-3.43|P-value:1.32E-4 ID:SGTC_2537|Compound:larixol|FD-Score:3.96|P-value:1.07E-5 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:-3.35|P-value:1.84E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:3.64|P-value:4.70E-5 ID:SGTC_2807|Compound:7945238|FD-Score:-4.80|P-value:1.63E-7 ID:SGTC_2830|Compound:7992007|FD-Score:-3.56|P-value:7.76E-5 ID:SGTC_3197|Compound:9113331|FD-Score:2.89|P-value:9.64E-4 ID:SGTC_3320|Compound:9137005|FD-Score:-3.41|P-value:1.46E-4 ID:SGTC_215|Compound:1287-0124|FD-Score:3.30|P-value:1.95E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:3.11|P-value:4.24E-4 ID:SGTC_374|Compound:0880-0231|FD-Score:4.92|P-value:6.52E-8 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:3.83|P-value:1.95E-5 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:3.64|P-value:4.61E-5 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.54|P-value:7.17E-5 ID:SGTC_782|Compound:0083-0095|FD-Score:-4.05|P-value:8.09E-6 ID:SGTC_785|Compound:1270-0111|FD-Score:-4.56|P-value:5.99E-7 ID:SGTC_812|Compound:4251-0442|FD-Score:2.94|P-value:7.81E-4 ID:SGTC_868|Compound:0527-0199|FD-Score:2.96|P-value:7.29E-4 ID:SGTC_898|Compound:1000-0849|FD-Score:3.70|P-value:3.61E-5 ID:SGTC_900|Compound:1112-0045|FD-Score:-3.81|P-value:2.55E-5 ID:SGTC_956|Compound:1185-0240|FD-Score:-3.09|P-value:5.03E-4 ID:SGTC_958|Compound:1222-0007|FD-Score:3.13|P-value:3.81E-4 ID:SGTC_1294|Compound:1000-1494|FD-Score:3.15|P-value:3.56E-4 ID:SGTC_1303|Compound:1160-0052|FD-Score:-4.33|P-value:2.01E-6 ID:SGTC_1311|Compound:1190-0148|FD-Score:3.23|P-value:2.62E-4 ID:SGTC_1312|Compound:1218-2183|FD-Score:4.01|P-value:8.47E-6 ID:SGTC_1692|Compound:fluoroquinolonic acid|FD-Score:4.26|P-value:2.47E-6 ID:SGTC_1927|Compound:st060202|FD-Score:3.11|P-value:4.17E-4 ID:SGTC_1948|Compound:st076329|FD-Score:2.88|P-value:9.75E-4 ID:SGTC_2160|Compound:5660466|FD-Score:3.20|P-value:3.01E-4 ID:SGTC_2238|Compound:6671620|FD-Score:-3.43|P-value:1.31E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-4.30|P-value:2.36E-6 ID:SGTC_2394|Compound:5221648|FD-Score:3.48|P-value:9.38E-5 ID:SGTC_2429|Compound:5902844|FD-Score:3.64|P-value:4.64E-5 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:-3.43|P-value:1.32E-4 ID:SGTC_2537|Compound:larixol|FD-Score:3.96|P-value:1.07E-5 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:-3.35|P-value:1.84E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:3.64|P-value:4.70E-5 ID:SGTC_2807|Compound:7945238|FD-Score:-4.80|P-value:1.63E-7 ID:SGTC_2830|Compound:7992007|FD-Score:-3.56|P-value:7.76E-5 ID:SGTC_3197|Compound:9113331|FD-Score:2.89|P-value:9.64E-4 ID:SGTC_3320|Compound:9137005|FD-Score:-3.41|P-value:1.46E-4

Top fitness defect scores for YLR154C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_374 0880-0231 4.92 82.3 µM 6.52E-8 iron homeostasis 8 4.22E-7
2 SGTC_1692 fluoroquinolonic acid 4.26 71.0 µM 2.47E-6 2 1.03E-5
3 SGTC_1312 1218-2183 4.01 165.0 µM 8.47E-6 6 3.03E-5
4 SGTC_2537 larixol 3.96 68.9 µM 1.07E-5 22 3.71E-5
5 SGTC_392 N-ethylmaleimide 3.83 8.3 µM 1.95E-5 RNA pol III & RNase P/MRP 17 6.31E-5
6 SGTC_898 1000-0849 3.70 28.3 µM 3.61E-5 7 1.08E-4
7 SGTC_474 dl-dihydrosphingosine 3.64 5.0 µM 4.61E-5 32 1.34E-4
8 SGTC_2429 5902844 3.64 64.4 µM 4.64E-5 34 1.35E-4
9 SGTC_2754 granisetron 3.64 64.0 µM 4.70E-5 42 1.36E-4
10 SGTC_682 0kpi-0099 3.54 17.0 µM 7.17E-5 87 1.98E-4
11 SGTC_2394 5221648 3.48 200.0 µM 9.38E-5 37 2.50E-4
12 SGTC_215 1287-0124 3.30 461.5 µM 1.95E-4 8 4.77E-4
13 SGTC_1311 1190-0148 3.23 100.0 µM 2.62E-4 azole & statin 50 6.18E-4
14 SGTC_2160 5660466 3.20 132.9 µM 3.01E-4 60S ribosome export 42 6.99E-4
15 SGTC_1294 1000-1494 3.15 117.0 µM 3.56E-4 51 8.09E-4
16 SGTC_958 1222-0007 3.13 33.0 µM 3.81E-4 57 8.60E-4
17 SGTC_1927 st060202 3.11 13.8 µM 4.17E-4 50 9.31E-4
18 SGTC_335 4077-0256 3.11 27.6 µM 4.24E-4 36 9.45E-4
19 SGTC_868 0527-0199 2.96 213.0 µM 7.29E-4 fatty acid desaturase (OLE1) 73 0.00152
20 SGTC_812 4251-0442 2.94 148.0 µM 7.81E-4 39 0.00162

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.184 7.41E-27 YPR011C_p YPR011C_p hom Putative transporter, member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.178 3.36E-25 YGL131C SNT2 hom DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.158 3.21E-20 YLR002C NOC3 het Component of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing
0.154 2.47E-19 YBL017C PEP1 hom Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments
0.150 2.38E-18 YML126C ERG13 het 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
0.150 2.85E-18 YDL207W GLE1 het Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export
0.146 1.67E-17 YBL001C ECM15 hom Non-essential protein of unknown function, likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis
0.141 2.82E-16 YDR107C TMN2 hom Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments
0.140 3.45E-16 YDL180W_p YDL180W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
0.140 4.35E-16 YJL051W IRC8 hom Bud tip localized protein of unknown function; mRNA is targeted to the bud by a She2p dependent transport system; mRNA is cell cycle regulated via Fkh2p, peaking in G2/M phase; null mutant displays increased levels of spontaneous Rad52p foc
0.137 1.67E-15 YJR129C_p YJR129C_p hom Putative protein of unknown function; predicted S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.136 2.71E-15 YGR292W MAL12 hom Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose
0.124 4.65E-13 YDR042C_p YDR042C_p hom Putative protein of unknown function; expression is increased in ssu72-ts69 mutant
0.120 2.74E-12 YMR173W DDR48 hom DNA damage-responsive protein; expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS; protein abundance increases in response to DNA replication stress
0.119 5.19E-12 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W