HIPHOP chemogenomics database

YLR199C / PBA1

Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly

Zygosity: Homozygous strain

fixedexpanded
Profile for YLR199C / PBA1

Click on Significant Values for Screen Details

Top fitness defect scores for YLR199C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_64	000l-0018	7.86	272.1 µM	6.19E-21	ubiquinone biosynthesis & proteasome	52	1.86E-15
2	SGTC_67	0335-0847	6.38	266.0 µM	2.12E-14	ubiquinone biosynthesis & proteasome	61	8.98E-11
3	SGTC_65	0798-0905	4.40	35.0 µM	9.33E-8	calcium & mitochondrial duress	67	5.39E-6
4	SGTC_1450	4112-4260	3.52	37.6 µM	1.57E-5	calcium & mitochondrial duress	42	2.20E-4
5	SGTC_1832	st054113	3.45	55.0 µM	2.23E-5		13	2.84E-4
6	SGTC_726	3448-4391	3.39	34.2 µM	2.97E-5		33	3.49E-4
7	SGTC_2602	kawain	2.96	100.0 µM	2.25E-4		80	0.00152
8	SGTC_3202	9113761	2.95	49.5 µM	2.41E-4		109	0.00161
9	SGTC_2060	5238662	2.88	140.0 µM	3.25E-4		69	0.00200
10	SGTC_463	ikarugamyin	2.87	104.0 µM	3.41E-4		19	0.00207
11	SGTC_1138	3761-0006	2.81	1.0 µM	4.35E-4		35	0.00247
12	SGTC_73	0327-0325	2.77	16.2 µM	5.28E-4	DNA damage response	146	0.00284
13	SGTC_2595	hexamethylquercetagetin	2.75	100.0 µM	5.74E-4		91	0.00302
14	SGTC_1332	1480-0653	2.74	17.5 µM	5.86E-4	TSC3-RPN4	82	0.00307
15	SGTC_2361	9034121	2.72	116.0 µM	6.33E-4	RSC complex & mRNA processing	78	0.00325
16	SGTC_669	0108-0021	2.69	26.2 µM	7.23E-4	fatty acid desaturase (OLE1)	183	0.00358
17	SGTC_3315	9136395	2.64	68.5 µM	8.79E-4		80	0.00413
18	SGTC_2050	5263056	2.63	171.0 µM	9.34E-4	fatty acid desaturase (OLE1)	136	0.00432
19	SGTC_763	3453-1109	2.59	128.0 µM	0.00106		99	0.00473
20	SGTC_2496	diffractaic acid	2.54	79.8 µM	0.00129		126	0.00547

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.315	2.84E-78	YMR110C	HFD1	hom	Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder
0.301	4.13E-71	YBR169C	SSE2	hom	Member of the heat shock protein 70 (HSP70) family; may be involved in protein folding; localized to the cytoplasm; SSE2 has a paralog, SSE1, that arose from the whole genome duplication
0.297	3.25E-69	YNL105W_d	RRT16_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription
0.295	1.97E-68	YPR024W	YME1	hom	Catalytic subunit of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; also has a role in intermembrane space protein folding; mutation causes an elevated rate of mitochondrial turnover
0.285	7.74E-64	YBR003W	COQ1	hom	Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis
0.282	2.09E-62	YPR146C_d	YPR146C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.264	8.25E-55	YJL168C	SET2	hom	Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p
0.255	4.02E-51	YNR042W_d	YNR042W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.248	2.43E-48	YDR148C	KGD2	hom	Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated
0.243	2.43E-46	YDR204W	COQ4	hom	Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex
0.233	1.61E-42	YML014W	TRM9	hom	tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses
0.231	9.14E-42	YLR127C	APC2	het	Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p
0.229	3.00E-41	YNL111C	CYB5	hom	Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation
0.227	1.29E-40	YLR239C	LIP2	hom	Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups
0.227	2.30E-40	YLR021W	IRC25	hom	Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions