YLR202C_d

Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR202C / YLR202C

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:8.40|P-value:3.01E-18 ID:SGTC_59|Compound:0330-0160|FD-Score:3.78|P-value:5.33E-5 ID:SGTC_64|Compound:000l-0018|FD-Score:7.88|P-value:2.79E-16 ID:SGTC_67|Compound:0335-0847|FD-Score:8.16|P-value:2.35E-17 ID:SGTC_86|Compound:0417-1630|FD-Score:26.80|P-value:2.14E-167 ID:SGTC_87|Compound:0100-0052|FD-Score:4.18|P-value:8.71E-6 ID:SGTC_100|Compound:0086-0128|FD-Score:3.25|P-value:4.32E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:4.80|P-value:4.04E-7 ID:SGTC_142|Compound:0335-0849|FD-Score:5.50|P-value:8.09E-9 ID:SGTC_158|Compound:k038-0087|FD-Score:8.81|P-value:6.41E-20 ID:SGTC_165|Compound:k038-0023|FD-Score:5.04|P-value:1.11E-7 ID:SGTC_184|Compound:1494-0393|FD-Score:3.09|P-value:7.72E-4 ID:SGTC_200|Compound:k038-0079|FD-Score:4.54|P-value:1.56E-6 ID:SGTC_216|Compound:k038-0035|FD-Score:5.57|P-value:5.18E-9 ID:SGTC_236|Compound:1509-0011|FD-Score:15.70|P-value:6.01E-59 ID:SGTC_275|Compound:trichlorophene|FD-Score:4.60|P-value:1.15E-6 ID:SGTC_310|Compound:1273-0058|FD-Score:4.21|P-value:7.63E-6 ID:SGTC_340|Compound:k038-0059|FD-Score:3.78|P-value:5.28E-5 ID:SGTC_386|Compound:1509-0011|FD-Score:5.45|P-value:1.06E-8 ID:SGTC_401|Compound:neomycin|FD-Score:4.20|P-value:7.97E-6 ID:SGTC_467|Compound:ro 31-8220|FD-Score:3.78|P-value:5.29E-5 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:-4.10|P-value:1.14E-5 ID:SGTC_481|Compound:bay k-8644|FD-Score:-4.25|P-value:5.55E-6 ID:SGTC_485|Compound:niflumic acid|FD-Score:6.78|P-value:1.55E-12 ID:SGTC_488|Compound:nitrendipine|FD-Score:-4.18|P-value:7.93E-6 ID:SGTC_492|Compound:tmb-8|FD-Score:3.21|P-value:5.03E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:3.33|P-value:3.16E-4 ID:SGTC_533|Compound:1222-0222|FD-Score:3.15|P-value:6.23E-4 ID:SGTC_583|Compound:1488-1091|FD-Score:3.17|P-value:5.83E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:3.24|P-value:4.44E-4 ID:SGTC_635|Compound:k038-0028|FD-Score:5.64|P-value:3.47E-9 ID:SGTC_762|Compound:1165-0429|FD-Score:-3.68|P-value:7.03E-5 ID:SGTC_767|Compound:0929-0023|FD-Score:3.38|P-value:2.67E-4 ID:SGTC_794|Compound:k038-0031|FD-Score:8.30|P-value:7.37E-18 ID:SGTC_846|Compound:0335-0850|FD-Score:3.45|P-value:1.97E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:-5.97|P-value:3.55E-10 ID:SGTC_923|Compound:1900-2434|FD-Score:4.78|P-value:4.64E-7 ID:SGTC_931|Compound:2812-0076|FD-Score:-3.69|P-value:6.98E-5 ID:SGTC_933|Compound:2914-0560|FD-Score:3.53|P-value:1.48E-4 ID:SGTC_938|Compound:3126-1755|FD-Score:3.28|P-value:3.82E-4 ID:SGTC_963|Compound:1269-2853|FD-Score:-2.99|P-value:9.83E-4 ID:SGTC_1017|Compound:4239-0030|FD-Score:3.46|P-value:1.90E-4 ID:SGTC_1075|Compound:aripiprazole|FD-Score:5.55|P-value:5.85E-9 ID:SGTC_1132|Compound:3456-3614|FD-Score:3.40|P-value:2.39E-4 ID:SGTC_1175|Compound:0741-0056|FD-Score:5.61|P-value:4.00E-9 ID:SGTC_1183|Compound:1254-0324|FD-Score:3.26|P-value:4.07E-4 ID:SGTC_1214|Compound:0358-0031|FD-Score:3.10|P-value:7.45E-4 ID:SGTC_1222|Compound:0366-0074|FD-Score:3.68|P-value:7.85E-5 ID:SGTC_1268|Compound:0828-0275|FD-Score:3.34|P-value:3.03E-4 ID:SGTC_1295|Compound:1013-0063|FD-Score:-3.45|P-value:1.81E-4 ID:SGTC_1319|Compound:1306-0155|FD-Score:4.56|P-value:1.45E-6 ID:SGTC_1367|Compound:1812-0510|FD-Score:4.43|P-value:2.74E-6 ID:SGTC_1374|Compound:2889-5571|FD-Score:-3.61|P-value:9.61E-5 ID:SGTC_1478|Compound:0453-0656|FD-Score:4.34|P-value:4.17E-6 ID:SGTC_1573|Compound:oleylamine|FD-Score:3.36|P-value:2.80E-4 ID:SGTC_1582|Compound:3',3',6'-trihydroxyflavone|FD-Score:3.10|P-value:7.29E-4 ID:SGTC_1611|Compound:st001856|FD-Score:5.44|P-value:1.15E-8 ID:SGTC_1635|Compound:st007035|FD-Score:5.03|P-value:1.20E-7 ID:SGTC_1721|Compound:st082152|FD-Score:3.38|P-value:2.60E-4 ID:SGTC_1776|Compound:st049249|FD-Score:3.34|P-value:3.02E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-5.16|P-value:4.97E-8 ID:SGTC_1796|Compound:st049173|FD-Score:3.72|P-value:6.76E-5 ID:SGTC_1799|Compound:st045822|FD-Score:3.02|P-value:9.91E-4 ID:SGTC_1909|Compound:st060837|FD-Score:-3.71|P-value:6.18E-5 ID:SGTC_2050|Compound:5263056|FD-Score:3.93|P-value:2.79E-5 ID:SGTC_2088|Compound:5278280|FD-Score:3.67|P-value:8.22E-5 ID:SGTC_2131|Compound:toluylene red|FD-Score:-4.08|P-value:1.27E-5 ID:SGTC_2333|Compound:9017922|FD-Score:5.42|P-value:1.32E-8 ID:SGTC_2342|Compound:7990637|FD-Score:-3.04|P-value:8.27E-4 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:3.06|P-value:8.44E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:3.49|P-value:1.70E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:-3.28|P-value:3.46E-4 ID:SGTC_2537|Compound:larixol|FD-Score:4.21|P-value:7.59E-6 ID:SGTC_2594|Compound:a-mangostin|FD-Score:3.16|P-value:5.86E-4 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:3.36|P-value:2.86E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-3.39|P-value:2.25E-4 ID:SGTC_2692|Compound:st077426|FD-Score:3.22|P-value:4.74E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:3.25|P-value:4.23E-4 ID:SGTC_2759|Compound:disulfiram|FD-Score:3.06|P-value:8.53E-4 ID:SGTC_2792|Compound:7535235|FD-Score:3.92|P-value:2.88E-5 ID:SGTC_2815|Compound:7968870|FD-Score:5.54|P-value:6.17E-9 ID:SGTC_2921|Compound:7974240|FD-Score:3.21|P-value:5.01E-4 ID:SGTC_2931|Compound:9014674|FD-Score:-3.17|P-value:5.25E-4 ID:SGTC_2971|Compound:9092186|FD-Score:-3.63|P-value:8.82E-5 ID:SGTC_3132|Compound:9126706|FD-Score:3.02|P-value:9.66E-4 ID:SGTC_3292|Compound:9120825|FD-Score:3.55|P-value:1.33E-4 ID:SGTC_3326|Compound:9140064|FD-Score:-3.91|P-value:2.71E-5 ID:SGTC_3336|Compound:9143619|FD-Score:5.33|P-value:2.18E-8 ID:SGTC_3346|Compound:9158254|FD-Score:3.31|P-value:3.49E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:8.40|P-value:3.01E-18 ID:SGTC_59|Compound:0330-0160|FD-Score:3.78|P-value:5.33E-5 ID:SGTC_64|Compound:000l-0018|FD-Score:7.88|P-value:2.79E-16 ID:SGTC_67|Compound:0335-0847|FD-Score:8.16|P-value:2.35E-17 ID:SGTC_86|Compound:0417-1630|FD-Score:26.80|P-value:2.14E-167 ID:SGTC_87|Compound:0100-0052|FD-Score:4.18|P-value:8.71E-6 ID:SGTC_100|Compound:0086-0128|FD-Score:3.25|P-value:4.32E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:4.80|P-value:4.04E-7 ID:SGTC_142|Compound:0335-0849|FD-Score:5.50|P-value:8.09E-9 ID:SGTC_158|Compound:k038-0087|FD-Score:8.81|P-value:6.41E-20 ID:SGTC_165|Compound:k038-0023|FD-Score:5.04|P-value:1.11E-7 ID:SGTC_184|Compound:1494-0393|FD-Score:3.09|P-value:7.72E-4 ID:SGTC_200|Compound:k038-0079|FD-Score:4.54|P-value:1.56E-6 ID:SGTC_216|Compound:k038-0035|FD-Score:5.57|P-value:5.18E-9 ID:SGTC_236|Compound:1509-0011|FD-Score:15.70|P-value:6.01E-59 ID:SGTC_275|Compound:trichlorophene|FD-Score:4.60|P-value:1.15E-6 ID:SGTC_310|Compound:1273-0058|FD-Score:4.21|P-value:7.63E-6 ID:SGTC_340|Compound:k038-0059|FD-Score:3.78|P-value:5.28E-5 ID:SGTC_386|Compound:1509-0011|FD-Score:5.45|P-value:1.06E-8 ID:SGTC_401|Compound:neomycin|FD-Score:4.20|P-value:7.97E-6 ID:SGTC_467|Compound:ro 31-8220|FD-Score:3.78|P-value:5.29E-5 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:-4.10|P-value:1.14E-5 ID:SGTC_481|Compound:bay k-8644|FD-Score:-4.25|P-value:5.55E-6 ID:SGTC_485|Compound:niflumic acid|FD-Score:6.78|P-value:1.55E-12 ID:SGTC_488|Compound:nitrendipine|FD-Score:-4.18|P-value:7.93E-6 ID:SGTC_492|Compound:tmb-8|FD-Score:3.21|P-value:5.03E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:3.33|P-value:3.16E-4 ID:SGTC_533|Compound:1222-0222|FD-Score:3.15|P-value:6.23E-4 ID:SGTC_583|Compound:1488-1091|FD-Score:3.17|P-value:5.83E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:3.24|P-value:4.44E-4 ID:SGTC_635|Compound:k038-0028|FD-Score:5.64|P-value:3.47E-9 ID:SGTC_762|Compound:1165-0429|FD-Score:-3.68|P-value:7.03E-5 ID:SGTC_767|Compound:0929-0023|FD-Score:3.38|P-value:2.67E-4 ID:SGTC_794|Compound:k038-0031|FD-Score:8.30|P-value:7.37E-18 ID:SGTC_846|Compound:0335-0850|FD-Score:3.45|P-value:1.97E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:-5.97|P-value:3.55E-10 ID:SGTC_923|Compound:1900-2434|FD-Score:4.78|P-value:4.64E-7 ID:SGTC_931|Compound:2812-0076|FD-Score:-3.69|P-value:6.98E-5 ID:SGTC_933|Compound:2914-0560|FD-Score:3.53|P-value:1.48E-4 ID:SGTC_938|Compound:3126-1755|FD-Score:3.28|P-value:3.82E-4 ID:SGTC_963|Compound:1269-2853|FD-Score:-2.99|P-value:9.83E-4 ID:SGTC_1017|Compound:4239-0030|FD-Score:3.46|P-value:1.90E-4 ID:SGTC_1075|Compound:aripiprazole|FD-Score:5.55|P-value:5.85E-9 ID:SGTC_1132|Compound:3456-3614|FD-Score:3.40|P-value:2.39E-4 ID:SGTC_1175|Compound:0741-0056|FD-Score:5.61|P-value:4.00E-9 ID:SGTC_1183|Compound:1254-0324|FD-Score:3.26|P-value:4.07E-4 ID:SGTC_1214|Compound:0358-0031|FD-Score:3.10|P-value:7.45E-4 ID:SGTC_1222|Compound:0366-0074|FD-Score:3.68|P-value:7.85E-5 ID:SGTC_1268|Compound:0828-0275|FD-Score:3.34|P-value:3.03E-4 ID:SGTC_1295|Compound:1013-0063|FD-Score:-3.45|P-value:1.81E-4 ID:SGTC_1319|Compound:1306-0155|FD-Score:4.56|P-value:1.45E-6 ID:SGTC_1367|Compound:1812-0510|FD-Score:4.43|P-value:2.74E-6 ID:SGTC_1374|Compound:2889-5571|FD-Score:-3.61|P-value:9.61E-5 ID:SGTC_1478|Compound:0453-0656|FD-Score:4.34|P-value:4.17E-6 ID:SGTC_1573|Compound:oleylamine|FD-Score:3.36|P-value:2.80E-4 ID:SGTC_1582|Compound:3',3',6'-trihydroxyflavone|FD-Score:3.10|P-value:7.29E-4 ID:SGTC_1611|Compound:st001856|FD-Score:5.44|P-value:1.15E-8 ID:SGTC_1635|Compound:st007035|FD-Score:5.03|P-value:1.20E-7 ID:SGTC_1721|Compound:st082152|FD-Score:3.38|P-value:2.60E-4 ID:SGTC_1776|Compound:st049249|FD-Score:3.34|P-value:3.02E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-5.16|P-value:4.97E-8 ID:SGTC_1796|Compound:st049173|FD-Score:3.72|P-value:6.76E-5 ID:SGTC_1799|Compound:st045822|FD-Score:3.02|P-value:9.91E-4 ID:SGTC_1909|Compound:st060837|FD-Score:-3.71|P-value:6.18E-5 ID:SGTC_2050|Compound:5263056|FD-Score:3.93|P-value:2.79E-5 ID:SGTC_2088|Compound:5278280|FD-Score:3.67|P-value:8.22E-5 ID:SGTC_2131|Compound:toluylene red|FD-Score:-4.08|P-value:1.27E-5 ID:SGTC_2333|Compound:9017922|FD-Score:5.42|P-value:1.32E-8 ID:SGTC_2342|Compound:7990637|FD-Score:-3.04|P-value:8.27E-4 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:3.06|P-value:8.44E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:3.49|P-value:1.70E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:-3.28|P-value:3.46E-4 ID:SGTC_2537|Compound:larixol|FD-Score:4.21|P-value:7.59E-6 ID:SGTC_2594|Compound:a-mangostin|FD-Score:3.16|P-value:5.86E-4 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:3.36|P-value:2.86E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-3.39|P-value:2.25E-4 ID:SGTC_2692|Compound:st077426|FD-Score:3.22|P-value:4.74E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:3.25|P-value:4.23E-4 ID:SGTC_2759|Compound:disulfiram|FD-Score:3.06|P-value:8.53E-4 ID:SGTC_2792|Compound:7535235|FD-Score:3.92|P-value:2.88E-5 ID:SGTC_2815|Compound:7968870|FD-Score:5.54|P-value:6.17E-9 ID:SGTC_2921|Compound:7974240|FD-Score:3.21|P-value:5.01E-4 ID:SGTC_2931|Compound:9014674|FD-Score:-3.17|P-value:5.25E-4 ID:SGTC_2971|Compound:9092186|FD-Score:-3.63|P-value:8.82E-5 ID:SGTC_3132|Compound:9126706|FD-Score:3.02|P-value:9.66E-4 ID:SGTC_3292|Compound:9120825|FD-Score:3.55|P-value:1.33E-4 ID:SGTC_3326|Compound:9140064|FD-Score:-3.91|P-value:2.71E-5 ID:SGTC_3336|Compound:9143619|FD-Score:5.33|P-value:2.18E-8 ID:SGTC_3346|Compound:9158254|FD-Score:3.31|P-value:3.49E-4

Top fitness defect scores for YLR202C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_86 0417-1630 26.80 471.9 µM 2.14E-167 ubiquinone biosynthesis & proteasome 1 3.84E-158
2 SGTC_236 1509-0011 15.70 5.3 µM 6.01E-59 91 8.49E-56
3 SGTC_158 k038-0087 8.81 26.4 µM 6.41E-20 ubiquinone biosynthesis & proteasome 38 6.10E-19
4 SGTC_36 0335-0881 8.40 14.2 µM 3.01E-18 ubiquinone biosynthesis & proteasome 34 2.32E-17
5 SGTC_794 k038-0031 8.30 316.0 µM 7.37E-18 ubiquinone biosynthesis & proteasome 28 5.41E-17
6 SGTC_67 0335-0847 8.16 266.0 µM 2.35E-17 ubiquinone biosynthesis & proteasome 38 1.62E-16
7 SGTC_64 000l-0018 7.88 272.1 µM 2.79E-16 ubiquinone biosynthesis & proteasome 50 1.68E-15
8 SGTC_485 niflumic acid 6.78 177.0 µM 1.55E-12 1 5.82E-12
9 SGTC_635 k038-0028 5.64 24.7 µM 3.47E-9 ubiquinone biosynthesis & proteasome 19 8.61E-9
10 SGTC_1175 0741-0056 5.61 27.6 µM 4.00E-9 3 9.85E-9
11 SGTC_216 k038-0035 5.57 28.4 µM 5.18E-9 ubiquinone biosynthesis & proteasome 29 1.26E-8
12 SGTC_1075 aripiprazole 5.55 15.2 µM 5.85E-9 5 1.41E-8
13 SGTC_2815 7968870 5.54 71.4 µM 6.17E-9 5 1.49E-8
14 SGTC_142 0335-0849 5.50 62.7 µM 8.09E-9 ubiquinone biosynthesis & proteasome 73 1.92E-8
15 SGTC_386 1509-0011 5.45 3.1 µM 1.06E-8 98 2.48E-8
16 SGTC_1611 st001856 5.44 99.4 µM 1.15E-8 4 2.68E-8
17 SGTC_2333 9017922 5.42 200.0 µM 1.32E-8 10 3.05E-8
18 SGTC_3336 9143619 5.33 71.4 µM 2.18E-8 4 4.90E-8
19 SGTC_165 k038-0023 5.04 64.4 µM 1.11E-7 ubiquinone biosynthesis & proteasome 40 2.29E-7
20 SGTC_1635 st007035 5.03 42.4 µM 1.20E-7 165 2.47E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.653 0 YLR201C COQ9 hom Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes
0.648 0 YNR041C COQ2 hom Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis
0.634 0 YDR204W COQ4 hom Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex
0.607 0 YOR125C CAT5 hom Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation
0.565 9.83E-282 YNR042W_d YNR042W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.547 1.52E-260 YGL119W COQ8 hom Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication
0.539 3.79E-252 YOL008W COQ10 hom Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q6 to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes
0.534 1.20E-246 YOL096C COQ3 hom O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein
0.532 6.96E-244 YGR255C COQ6 hom Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS)
0.522 2.28E-233 YBR003W COQ1 hom Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis
0.510 1.09E-221 YOR254C SEC63 het Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
0.496 2.46E-207 YML110C COQ5 hom 2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes
0.452 7.33E-168 YDR148C KGD2 hom Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated
0.437 2.95E-156 YML034C-A_d YML034C-A_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.421 2.23E-144 YCL014W BUD3 hom Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding