HIPHOP chemogenomics database

YLR291C / GCD7

Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression

Zygosity: Heterozygous strain

fixedexpanded
Profile for YLR291C / GCD7

Click on Significant Values for Screen Details

Top fitness defect scores for YLR291C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_392	N-ethylmaleimide	16.70	8.3 µM	2.83E-67	RNA pol III & RNase P/MRP	1	6.59E-63	5.03
2	SGTC_2411	st077133	13.50	39.9 µM	1.54E-44	RNA pol III & RNase P/MRP	6	1.19E-41	2.59
3	SGTC_1872	amphotericin b	8.27	148.0 nM	4.69E-18	RNA pol III & RNase P/MRP	8	6.60E-17	0.76
4	SGTC_1134	3477-0009	8.00	42.5 µM	5.16E-17		2	6.20E-16	1.30
5	SGTC_2612	dictamnine	7.79	15.5 µM	3.15E-16	RNA pol III & RNase P/MRP	13	3.36E-15	0.39
6	SGTC_2430	5571206	6.57	200.0 µM	4.43E-12		6	2.50E-11	0.46
7	SGTC_1130	3454-2858	6.32	15.9 µM	2.55E-11	NEO1	4	1.28E-10	0.49
8	SGTC_2387	9077560	5.73	195.1 µM	1.27E-9		5	4.91E-9	0.37
9	SGTC_2679	rapamycin	5.26	1.0 nM	2.31E-8		31	7.37E-8	0.02
10	SGTC_3077	9117319	4.70	49.5 µM	5.08E-7		5	1.32E-6	0.36
11	SGTC_87	0100-0052	4.48	35.5 µM	1.51E-6		14	3.65E-6	0.28
12	SGTC_2333	9017922	4.44	200.0 µM	1.90E-6		4	4.52E-6	0.19
13	SGTC_3223	9129970	4.37	49.5 µM	2.65E-6		3	6.14E-6	0.27
14	SGTC_539	4859-0462	4.32	2.5 µM	3.35E-6	RNA pol III & RNase P/MRP	17	7.65E-6	0.04
15	SGTC_611	3453-1652	4.12	23.7 µM	8.80E-6		20	1.88E-5	0.14
16	SGTC_705	1124-0212	4.00	310.0 µM	1.57E-5		6	3.23E-5	0.09
17	SGTC_207	4185-0010	3.60	4.5 µM	8.51E-5	endomembrane recycling	19	1.56E-4	0.06
18	SGTC_2332	7982454	3.40	138.9 µM	1.94E-4		16	3.37E-4	0.09
19	SGTC_627	1391-0720	3.33	175.0 µM	2.55E-4		8	4.34E-4	0.02
20	SGTC_2004	4023728	3.27	41.9 µM	3.23E-4		37	5.42E-4	0.00

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.515	1.08E-226	YGR083C	GCD2	het	Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.444	6.51E-162	YNL221C	POP1	het	Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
0.411	5.37E-137	YOR260W	GCD1	het	Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.331	1.22E-86	YDR211W	GCD6	het	Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress
0.236	1.26E-43	YBR167C	POP7	het	Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
0.200	1.56E-31	YOR207C	RET1	het	Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
0.184	7.14E-27	YMR283C	RIT1	hom	2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA
0.181	4.78E-26	YBR257W	POP4	het	Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
0.179	1.46E-25	YOR116C	RPO31	het	RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
0.174	3.27E-24	YDL152W_d	YDL152W_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
0.171	2.05E-23	YDL150W	RPC53	het	RNA polymerase III subunit C53
0.168	1.22E-22	YLR243W	GPN3	het	Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p
0.165	6.64E-22	YKL203C	TOR2	het	PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.138	1.20E-15	YDR527W	RBA50	het	Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
0.130	4.45E-14	YHR021W-A_p	ECM12_p	hom	Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity