YLR309C / IMH1

Protein involved in vesicular transport, mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR309C / IMH1

Click on Significant Values for Screen Details ID:SGTC_56|Compound:0957-0365|FD-Score:3.92|P-value:1.68E-5 ID:SGTC_89|Compound:0302-0250|FD-Score:3.23|P-value:3.12E-4 ID:SGTC_119|Compound:0121-0043|FD-Score:3.35|P-value:1.92E-4 ID:SGTC_124|Compound:0136-0217|FD-Score:3.04|P-value:6.24E-4 ID:SGTC_175|Compound:3793-3120|FD-Score:3.02|P-value:6.84E-4 ID:SGTC_276|Compound:colchicine|FD-Score:4.05|P-value:9.30E-6 ID:SGTC_284|Compound:0988-0104|FD-Score:3.17|P-value:3.85E-4 ID:SGTC_291|Compound:4130-1278|FD-Score:4.19|P-value:4.68E-6 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.51|P-value:1.23E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.03|P-value:1.30E-5 ID:SGTC_430|Compound:0958-0115|FD-Score:-4.52|P-value:1.15E-6 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:3.06|P-value:5.79E-4 ID:SGTC_438|Compound:sphingosine|FD-Score:-4.36|P-value:2.62E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.02|P-value:8.27E-4 ID:SGTC_500|Compound:h-89|FD-Score:2.95|P-value:8.85E-4 ID:SGTC_512|Compound:prima-1|FD-Score:3.71|P-value:4.32E-5 ID:SGTC_525|Compound:k781-2675|FD-Score:3.19|P-value:3.67E-4 ID:SGTC_577|Compound:r071-0003|FD-Score:3.47|P-value:1.17E-4 ID:SGTC_988|Compound:1497-1057|FD-Score:3.03|P-value:6.61E-4 ID:SGTC_1231|Compound:0242-0792|FD-Score:3.19|P-value:3.64E-4 ID:SGTC_1306|Compound:1185-0270|FD-Score:10.10|P-value:5.41E-27 ID:SGTC_1316|Compound:1272-0050|FD-Score:3.43|P-value:1.42E-4 ID:SGTC_1524|Compound:corynanthine|FD-Score:6.24|P-value:2.19E-11 ID:SGTC_1607|Compound:st000305|FD-Score:-3.51|P-value:1.24E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:6.78|P-value:4.20E-13 ID:SGTC_1696|Compound:st025591|FD-Score:4.25|P-value:3.51E-6 ID:SGTC_1829|Compound:berberine|FD-Score:-4.01|P-value:1.38E-5 ID:SGTC_1841|Compound:st054148|FD-Score:3.66|P-value:5.37E-5 ID:SGTC_1885|Compound:st057546|FD-Score:3.73|P-value:3.90E-5 ID:SGTC_2051|Compound:5263410|FD-Score:-3.20|P-value:4.19E-4 ID:SGTC_2398|Compound:5228213|FD-Score:2.95|P-value:8.84E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:3.07|P-value:5.69E-4 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:3.04|P-value:6.39E-4 ID:SGTC_2685|Compound:mycophenolic acid|FD-Score:3.61|P-value:6.54E-5 ID:SGTC_2689|Compound:tacrolimus|FD-Score:-2.97|P-value:9.93E-4 ID:SGTC_2770|Compound:toltrazuril|FD-Score:3.72|P-value:4.07E-5 ID:SGTC_3003|Compound:9075612|FD-Score:3.35|P-value:1.90E-4 ID:SGTC_3330|Compound:9141424|FD-Score:5.42|P-value:5.04E-9 ID:SGTC_56|Compound:0957-0365|FD-Score:3.92|P-value:1.68E-5 ID:SGTC_89|Compound:0302-0250|FD-Score:3.23|P-value:3.12E-4 ID:SGTC_119|Compound:0121-0043|FD-Score:3.35|P-value:1.92E-4 ID:SGTC_124|Compound:0136-0217|FD-Score:3.04|P-value:6.24E-4 ID:SGTC_175|Compound:3793-3120|FD-Score:3.02|P-value:6.84E-4 ID:SGTC_276|Compound:colchicine|FD-Score:4.05|P-value:9.30E-6 ID:SGTC_284|Compound:0988-0104|FD-Score:3.17|P-value:3.85E-4 ID:SGTC_291|Compound:4130-1278|FD-Score:4.19|P-value:4.68E-6 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.51|P-value:1.23E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.03|P-value:1.30E-5 ID:SGTC_430|Compound:0958-0115|FD-Score:-4.52|P-value:1.15E-6 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:3.06|P-value:5.79E-4 ID:SGTC_438|Compound:sphingosine|FD-Score:-4.36|P-value:2.62E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.02|P-value:8.27E-4 ID:SGTC_500|Compound:h-89|FD-Score:2.95|P-value:8.85E-4 ID:SGTC_512|Compound:prima-1|FD-Score:3.71|P-value:4.32E-5 ID:SGTC_525|Compound:k781-2675|FD-Score:3.19|P-value:3.67E-4 ID:SGTC_577|Compound:r071-0003|FD-Score:3.47|P-value:1.17E-4 ID:SGTC_988|Compound:1497-1057|FD-Score:3.03|P-value:6.61E-4 ID:SGTC_1231|Compound:0242-0792|FD-Score:3.19|P-value:3.64E-4 ID:SGTC_1306|Compound:1185-0270|FD-Score:10.10|P-value:5.41E-27 ID:SGTC_1316|Compound:1272-0050|FD-Score:3.43|P-value:1.42E-4 ID:SGTC_1524|Compound:corynanthine|FD-Score:6.24|P-value:2.19E-11 ID:SGTC_1607|Compound:st000305|FD-Score:-3.51|P-value:1.24E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:6.78|P-value:4.20E-13 ID:SGTC_1696|Compound:st025591|FD-Score:4.25|P-value:3.51E-6 ID:SGTC_1829|Compound:berberine|FD-Score:-4.01|P-value:1.38E-5 ID:SGTC_1841|Compound:st054148|FD-Score:3.66|P-value:5.37E-5 ID:SGTC_1885|Compound:st057546|FD-Score:3.73|P-value:3.90E-5 ID:SGTC_2051|Compound:5263410|FD-Score:-3.20|P-value:4.19E-4 ID:SGTC_2398|Compound:5228213|FD-Score:2.95|P-value:8.84E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:3.07|P-value:5.69E-4 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:3.04|P-value:6.39E-4 ID:SGTC_2685|Compound:mycophenolic acid|FD-Score:3.61|P-value:6.54E-5 ID:SGTC_2689|Compound:tacrolimus|FD-Score:-2.97|P-value:9.93E-4 ID:SGTC_2770|Compound:toltrazuril|FD-Score:3.72|P-value:4.07E-5 ID:SGTC_3003|Compound:9075612|FD-Score:3.35|P-value:1.90E-4 ID:SGTC_3330|Compound:9141424|FD-Score:5.42|P-value:5.04E-9

Top fitness defect scores for YLR309C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1306 1185-0270 10.10 9.0 µM 5.41E-27 Golgi 11 1.69E-24
2 SGTC_1679 4130-1276 6.78 6.7 µM 4.20E-13 Golgi 19 5.99E-12
3 SGTC_1524 corynanthine 6.24 62.1 µM 2.19E-11 4 2.13E-10
4 SGTC_3330 9141424 5.42 71.4 µM 5.04E-9 amide catabolism 7 2.90E-8
5 SGTC_1696 st025591 4.25 34.8 µM 3.51E-6 SWF1 & branched chain AA biosynthesis 21 1.08E-5
6 SGTC_291 4130-1278 4.19 35.5 µM 4.68E-6 Golgi 9 1.40E-5
7 SGTC_276 colchicine 4.05 750.0 µM 9.30E-6 15 2.60E-5
8 SGTC_56 0957-0365 3.92 83.2 µM 1.68E-5 Golgi 48 4.44E-5
9 SGTC_1885 st057546 3.73 99.9 µM 3.90E-5 26 9.50E-5
10 SGTC_2770 toltrazuril 3.72 17.5 µM 4.07E-5 34 9.86E-5
11 SGTC_512 prima-1 3.71 270.0 µM 4.32E-5 DNA intercalators 30 1.04E-4
12 SGTC_1841 st054148 3.66 70.0 µM 5.37E-5 15 1.27E-4
13 SGTC_2685 mycophenolic acid 3.61 18.9 µM 6.54E-5 RNA pol III & RNase P/MRP 9 1.51E-4
14 SGTC_577 r071-0003 3.47 38.6 µM 1.17E-4 fatty acid desaturase (OLE1) 80 2.56E-4
15 SGTC_1316 1272-0050 3.43 65.9 µM 1.42E-4 19 3.05E-4
16 SGTC_3003 9075612 3.35 71.4 µM 1.90E-4 39 3.97E-4
17 SGTC_119 0121-0043 3.35 24.8 µM 1.92E-4 mitochondrial stress 156 4.01E-4
18 SGTC_89 0302-0250 3.23 142.1 µM 3.12E-4 52 6.22E-4
19 SGTC_1231 0242-0792 3.19 125.0 µM 3.64E-4 47 7.15E-4
20 SGTC_525 k781-2675 3.19 26.7 µM 3.67E-4 redox potentiating 84 7.21E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.230 1.43E-41 YMR079W SEC14 het Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs
0.189 2.13E-28 YPL051W ARL3 hom GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor
0.173 5.08E-24 YBR164C ARL1 hom Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor
0.169 5.63E-23 YJL004C SYS1 hom Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
0.164 1.32E-21 YPR175W DPB2 het Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate
0.163 2.64E-21 YDR108W TRS85 hom Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role
0.160 1.09E-20 YPR176C BET2 het Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
0.140 3.65E-16 YJL032W_d YJL032W_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase
0.125 4.10E-13 YIR009W MSL1 hom U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members
0.123 1.02E-12 YGL065C ALG2 het Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol
0.118 6.30E-12 YJL085W EXO70 het Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.117 1.09E-11 YGL005C COG7 hom Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.116 1.35E-11 YLR190W MMR1 hom Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p
0.116 1.67E-11 YPR055W SEC8 het Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress
0.115 2.00E-11 YOR089C VPS21 hom Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication