YLR396C / VPS33

ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR396C / VPS33

Click on Significant Values for Screen Details ID:SGTC_40|Compound:0327-0103|FD-Score:-4.98|P-value:6.51E-7 ID:SGTC_57|Compound:miconazole|FD-Score:-3.63|P-value:2.04E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:-3.17|P-value:9.94E-4 ID:SGTC_152|Compound:4469-0003|FD-Score:-3.96|P-value:5.91E-5 ID:SGTC_156|Compound:3474-0145|FD-Score:-3.47|P-value:3.54E-4 ID:SGTC_172|Compound:1112-0046|FD-Score:4.03|P-value:6.16E-5 ID:SGTC_181|Compound:1447-1644|FD-Score:3.54|P-value:3.82E-4 ID:SGTC_217|Compound:ketoconazole|FD-Score:-3.30|P-value:6.39E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:4.21|P-value:3.08E-5 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.19|P-value:9.35E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.49|P-value:6.38E-6 ID:SGTC_312|Compound:4092-0653|FD-Score:-4.82|P-value:1.42E-6 ID:SGTC_313|Compound:3474-0040|FD-Score:4.11|P-value:4.64E-5 ID:SGTC_314|Compound:1671-0008|FD-Score:-6.03|P-value:2.48E-9 ID:SGTC_320|Compound:4092-0846|FD-Score:-5.33|P-value:1.13E-7 ID:SGTC_346|Compound:4513-0042|FD-Score:-5.50|P-value:4.75E-8 ID:SGTC_356|Compound:1634-0012|FD-Score:3.27|P-value:9.42E-4 ID:SGTC_435|Compound:c-PAF|FD-Score:-3.59|P-value:2.30E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.55|P-value:2.72E-4 ID:SGTC_635|Compound:k038-0028|FD-Score:-3.38|P-value:4.89E-4 ID:SGTC_648|Compound:0375-0473|FD-Score:3.70|P-value:2.21E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:-3.23|P-value:8.04E-4 ID:SGTC_942|Compound:3253-1115|FD-Score:3.74|P-value:1.88E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:3.64|P-value:2.74E-4 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:-3.30|P-value:6.33E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:-3.77|P-value:1.19E-4 ID:SGTC_1414|Compound:3917-0015|FD-Score:-3.53|P-value:2.92E-4 ID:SGTC_1547|Compound:harmine|FD-Score:4.23|P-value:2.86E-5 ID:SGTC_1550|Compound:gossypolone|FD-Score:4.48|P-value:9.84E-6 ID:SGTC_1873|Compound:rapamycin|FD-Score:3.30|P-value:8.74E-4 ID:SGTC_2013|Compound:4024628|FD-Score:4.56|P-value:6.87E-6 ID:SGTC_2242|Compound:6630449|FD-Score:4.22|P-value:2.90E-5 ID:SGTC_2268|Compound:7933382|FD-Score:3.59|P-value:3.23E-4 ID:SGTC_2293|Compound:7624732|FD-Score:3.55|P-value:3.70E-4 ID:SGTC_2385|Compound:9074692|FD-Score:3.82|P-value:1.39E-4 ID:SGTC_2792|Compound:7535235|FD-Score:-3.36|P-value:5.26E-4 ID:SGTC_2823|Compound:7987624|FD-Score:-3.28|P-value:6.82E-4 ID:SGTC_2839|Compound:9003951|FD-Score:-3.90|P-value:7.44E-5 ID:SGTC_2843|Compound:9000894|FD-Score:-6.63|P-value:6.72E-11 ID:SGTC_2845|Compound:9003024|FD-Score:-4.32|P-value:1.32E-5 ID:SGTC_2848|Compound:9016370|FD-Score:3.47|P-value:4.89E-4 ID:SGTC_2952|Compound:9073731|FD-Score:3.38|P-value:6.57E-4 ID:SGTC_2980|Compound:9014342|FD-Score:-3.58|P-value:2.46E-4 ID:SGTC_3108|Compound:9121785|FD-Score:-3.78|P-value:1.17E-4 ID:SGTC_3162|Compound:9099653|FD-Score:-3.22|P-value:8.45E-4 ID:SGTC_40|Compound:0327-0103|FD-Score:-4.98|P-value:6.51E-7 ID:SGTC_57|Compound:miconazole|FD-Score:-3.63|P-value:2.04E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:-3.17|P-value:9.94E-4 ID:SGTC_152|Compound:4469-0003|FD-Score:-3.96|P-value:5.91E-5 ID:SGTC_156|Compound:3474-0145|FD-Score:-3.47|P-value:3.54E-4 ID:SGTC_172|Compound:1112-0046|FD-Score:4.03|P-value:6.16E-5 ID:SGTC_181|Compound:1447-1644|FD-Score:3.54|P-value:3.82E-4 ID:SGTC_217|Compound:ketoconazole|FD-Score:-3.30|P-value:6.39E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:4.21|P-value:3.08E-5 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.19|P-value:9.35E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.49|P-value:6.38E-6 ID:SGTC_312|Compound:4092-0653|FD-Score:-4.82|P-value:1.42E-6 ID:SGTC_313|Compound:3474-0040|FD-Score:4.11|P-value:4.64E-5 ID:SGTC_314|Compound:1671-0008|FD-Score:-6.03|P-value:2.48E-9 ID:SGTC_320|Compound:4092-0846|FD-Score:-5.33|P-value:1.13E-7 ID:SGTC_346|Compound:4513-0042|FD-Score:-5.50|P-value:4.75E-8 ID:SGTC_356|Compound:1634-0012|FD-Score:3.27|P-value:9.42E-4 ID:SGTC_435|Compound:c-PAF|FD-Score:-3.59|P-value:2.30E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.55|P-value:2.72E-4 ID:SGTC_635|Compound:k038-0028|FD-Score:-3.38|P-value:4.89E-4 ID:SGTC_648|Compound:0375-0473|FD-Score:3.70|P-value:2.21E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:-3.23|P-value:8.04E-4 ID:SGTC_942|Compound:3253-1115|FD-Score:3.74|P-value:1.88E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:3.64|P-value:2.74E-4 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:-3.30|P-value:6.33E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:-3.77|P-value:1.19E-4 ID:SGTC_1414|Compound:3917-0015|FD-Score:-3.53|P-value:2.92E-4 ID:SGTC_1547|Compound:harmine|FD-Score:4.23|P-value:2.86E-5 ID:SGTC_1550|Compound:gossypolone|FD-Score:4.48|P-value:9.84E-6 ID:SGTC_1873|Compound:rapamycin|FD-Score:3.30|P-value:8.74E-4 ID:SGTC_2013|Compound:4024628|FD-Score:4.56|P-value:6.87E-6 ID:SGTC_2242|Compound:6630449|FD-Score:4.22|P-value:2.90E-5 ID:SGTC_2268|Compound:7933382|FD-Score:3.59|P-value:3.23E-4 ID:SGTC_2293|Compound:7624732|FD-Score:3.55|P-value:3.70E-4 ID:SGTC_2385|Compound:9074692|FD-Score:3.82|P-value:1.39E-4 ID:SGTC_2792|Compound:7535235|FD-Score:-3.36|P-value:5.26E-4 ID:SGTC_2823|Compound:7987624|FD-Score:-3.28|P-value:6.82E-4 ID:SGTC_2839|Compound:9003951|FD-Score:-3.90|P-value:7.44E-5 ID:SGTC_2843|Compound:9000894|FD-Score:-6.63|P-value:6.72E-11 ID:SGTC_2845|Compound:9003024|FD-Score:-4.32|P-value:1.32E-5 ID:SGTC_2848|Compound:9016370|FD-Score:3.47|P-value:4.89E-4 ID:SGTC_2952|Compound:9073731|FD-Score:3.38|P-value:6.57E-4 ID:SGTC_2980|Compound:9014342|FD-Score:-3.58|P-value:2.46E-4 ID:SGTC_3108|Compound:9121785|FD-Score:-3.78|P-value:1.17E-4 ID:SGTC_3162|Compound:9099653|FD-Score:-3.22|P-value:8.45E-4

Top fitness defect scores for YLR396C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2013 4024628 4.56 200.0 µM 6.87E-6 31 2.53E-6
2 SGTC_1550 gossypolone 4.48 36.6 µM 9.84E-6 6 3.74E-6
3 SGTC_1547 harmine 4.23 94.2 µM 2.86E-5 ergosterol biosynthesis 33 1.19E-5
4 SGTC_2242 6630449 4.22 61.3 µM 2.90E-5 Golgi 40 1.21E-5
5 SGTC_245 methotrexate 4.21 289.6 µM 3.08E-5 39 1.29E-5
6 SGTC_313 3474-0040 4.11 5.0 µM 4.64E-5 NEO1-PIK1 65 2.01E-5
7 SGTC_172 1112-0046 4.03 2.6 µM 6.16E-5 NEO1 35 2.74E-5
8 SGTC_2385 9074692 3.82 200.0 µM 1.39E-4 48 6.63E-5
9 SGTC_942 3253-1115 3.74 227.0 µM 1.88E-4 35 9.20E-5
10 SGTC_648 0375-0473 3.70 33.3 µM 2.21E-4 23 1.10E-4
11 SGTC_1130 3454-2858 3.64 15.9 µM 2.74E-4 NEO1 102 1.39E-4
12 SGTC_2268 7933382 3.59 153.8 µM 3.23E-4 47 1.66E-4
13 SGTC_2293 7624732 3.55 200.0 µM 3.70E-4 38 1.92E-4
14 SGTC_181 1447-1644 3.54 8.1 µM 3.82E-4 endomembrane recycling 46 1.99E-4
15 SGTC_2848 9016370 3.47 26.0 µM 4.89E-4 46 2.60E-4
16 SGTC_2952 9073731 3.38 13.8 µM 6.57E-4 tubulin folding & SWR complex 72 3.58E-4
17 SGTC_1873 rapamycin 3.30 2.0 nM 8.74E-4 98 4.89E-4
18 SGTC_356 1634-0012 3.27 32.5 µM 9.42E-4 51 5.31E-4
19 SGTC_748 0136-0228 3.23 77.9 µM 0.00108 redox potentiating 113 6.17E-4
20 SGTC_1713 st033146 3.22 12.8 µM 0.00111 58 6.35E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.220 6.60E-38 YLR148W PEP3 hom Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis
0.190 1.97E-28 YKR001C VPS1 hom Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis
0.159 1.86E-20 YGL095C VPS45 hom Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
0.158 3.35E-20 YLR240W VPS34 hom Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery
0.140 4.88E-16 YDR484W VPS52 hom Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin
0.139 7.10E-16 YHR004C NEM1 hom Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard
0.136 2.08E-15 YDR323C PEP7 hom Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
0.135 4.37E-15 YOR089C VPS21 hom Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
0.130 3.69E-14 YDR495C VPS3 hom Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
0.128 9.59E-14 YJL124C LSM1 hom Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress
0.127 1.86E-13 YOR036W PEP12 hom Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
0.125 4.52E-13 YBL071W-A KTI11 hom Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs
0.124 5.08E-13 YGL071W AFT1 hom Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.122 1.11E-12 YCR077C PAT1 hom Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress
0.122 1.74E-12 YLR315W NKP2 hom Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2