YLR398C / SKI2

Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR398C / SKI2

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:7.01|P-value:7.14E-12 ID:SGTC_448|Compound:monastrol|FD-Score:4.94|P-value:9.49E-7 ID:SGTC_632|Compound:0781-2353|FD-Score:3.20|P-value:9.97E-4 ID:SGTC_656|Compound:4090-1979|FD-Score:3.71|P-value:1.76E-4 ID:SGTC_877|Compound:0771-0151|FD-Score:4.26|P-value:1.97E-5 ID:SGTC_880|Compound:0833-0487|FD-Score:4.08|P-value:4.12E-5 ID:SGTC_1018|Compound:4259-0013|FD-Score:3.73|P-value:1.63E-4 ID:SGTC_1071|Compound:trifluoperazine|FD-Score:5.97|P-value:4.43E-9 ID:SGTC_1100|Compound:amiodarone|FD-Score:3.44|P-value:4.59E-4 ID:SGTC_1705|Compound:st027868|FD-Score:-3.70|P-value:1.92E-4 ID:SGTC_1708|Compound:st033234|FD-Score:-3.66|P-value:2.18E-4 ID:SGTC_1725|Compound:st036284|FD-Score:3.49|P-value:3.81E-4 ID:SGTC_1727|Compound:st034309|FD-Score:3.28|P-value:7.67E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-3.84|P-value:1.14E-4 ID:SGTC_1871|Compound:st057448|FD-Score:6.65|P-value:7.51E-11 ID:SGTC_1924|Compound:st058459|FD-Score:4.08|P-value:4.17E-5 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-4.93|P-value:1.09E-6 ID:SGTC_1929|Compound:genistein trimethyl ether|FD-Score:3.84|P-value:1.05E-4 ID:SGTC_1932|Compound:st074806|FD-Score:4.36|P-value:1.32E-5 ID:SGTC_2024|Compound:5144227|FD-Score:-3.36|P-value:6.30E-4 ID:SGTC_2069|Compound:5228469|FD-Score:-3.89|P-value:9.52E-5 ID:SGTC_2084|Compound:5306078|FD-Score:-3.25|P-value:9.07E-4 ID:SGTC_2099|Compound:5377090|FD-Score:4.38|P-value:1.21E-5 ID:SGTC_2160|Compound:5660466|FD-Score:3.27|P-value:7.96E-4 ID:SGTC_2359|Compound:9039622|FD-Score:4.25|P-value:2.13E-5 ID:SGTC_2367|Compound:9070478|FD-Score:5.08|P-value:4.81E-7 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:4.12|P-value:3.57E-5 ID:SGTC_2504|Compound:purpurogallin|FD-Score:-4.39|P-value:1.27E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-3.33|P-value:6.97E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:6.51|P-value:1.76E-10 ID:SGTC_2759|Compound:disulfiram|FD-Score:-3.64|P-value:2.34E-4 ID:SGTC_2933|Compound:9026794|FD-Score:-3.27|P-value:8.59E-4 ID:SGTC_3095|Compound:9117207|FD-Score:-3.77|P-value:1.49E-4 ID:SGTC_3147|Compound:9097027|FD-Score:3.52|P-value:3.43E-4 ID:SGTC_3152|Compound:9099253|FD-Score:-4.87|P-value:1.48E-6 ID:SGTC_3153|Compound:9100258|FD-Score:-4.55|P-value:6.23E-6 ID:SGTC_6|Compound:0844-0013|FD-Score:7.01|P-value:7.14E-12 ID:SGTC_448|Compound:monastrol|FD-Score:4.94|P-value:9.49E-7 ID:SGTC_632|Compound:0781-2353|FD-Score:3.20|P-value:9.97E-4 ID:SGTC_656|Compound:4090-1979|FD-Score:3.71|P-value:1.76E-4 ID:SGTC_877|Compound:0771-0151|FD-Score:4.26|P-value:1.97E-5 ID:SGTC_880|Compound:0833-0487|FD-Score:4.08|P-value:4.12E-5 ID:SGTC_1018|Compound:4259-0013|FD-Score:3.73|P-value:1.63E-4 ID:SGTC_1071|Compound:trifluoperazine|FD-Score:5.97|P-value:4.43E-9 ID:SGTC_1100|Compound:amiodarone|FD-Score:3.44|P-value:4.59E-4 ID:SGTC_1705|Compound:st027868|FD-Score:-3.70|P-value:1.92E-4 ID:SGTC_1708|Compound:st033234|FD-Score:-3.66|P-value:2.18E-4 ID:SGTC_1725|Compound:st036284|FD-Score:3.49|P-value:3.81E-4 ID:SGTC_1727|Compound:st034309|FD-Score:3.28|P-value:7.67E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-3.84|P-value:1.14E-4 ID:SGTC_1871|Compound:st057448|FD-Score:6.65|P-value:7.51E-11 ID:SGTC_1924|Compound:st058459|FD-Score:4.08|P-value:4.17E-5 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-4.93|P-value:1.09E-6 ID:SGTC_1929|Compound:genistein trimethyl ether|FD-Score:3.84|P-value:1.05E-4 ID:SGTC_1932|Compound:st074806|FD-Score:4.36|P-value:1.32E-5 ID:SGTC_2024|Compound:5144227|FD-Score:-3.36|P-value:6.30E-4 ID:SGTC_2069|Compound:5228469|FD-Score:-3.89|P-value:9.52E-5 ID:SGTC_2084|Compound:5306078|FD-Score:-3.25|P-value:9.07E-4 ID:SGTC_2099|Compound:5377090|FD-Score:4.38|P-value:1.21E-5 ID:SGTC_2160|Compound:5660466|FD-Score:3.27|P-value:7.96E-4 ID:SGTC_2359|Compound:9039622|FD-Score:4.25|P-value:2.13E-5 ID:SGTC_2367|Compound:9070478|FD-Score:5.08|P-value:4.81E-7 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:4.12|P-value:3.57E-5 ID:SGTC_2504|Compound:purpurogallin|FD-Score:-4.39|P-value:1.27E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-3.33|P-value:6.97E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:6.51|P-value:1.76E-10 ID:SGTC_2759|Compound:disulfiram|FD-Score:-3.64|P-value:2.34E-4 ID:SGTC_2933|Compound:9026794|FD-Score:-3.27|P-value:8.59E-4 ID:SGTC_3095|Compound:9117207|FD-Score:-3.77|P-value:1.49E-4 ID:SGTC_3147|Compound:9097027|FD-Score:3.52|P-value:3.43E-4 ID:SGTC_3152|Compound:9099253|FD-Score:-4.87|P-value:1.48E-6 ID:SGTC_3153|Compound:9100258|FD-Score:-4.55|P-value:6.23E-6

Top fitness defect scores for YLR398C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_6 0844-0013 7.01 19.8 µM 7.14E-12 5 1.18E-12
2 SGTC_1871 st057448 6.65 67.3 µM 7.51E-11 2 1.49E-11
3 SGTC_2754 granisetron 6.51 64.0 µM 1.76E-10 4 3.72E-11
4 SGTC_1071 trifluoperazine 5.97 6.3 µM 4.43E-9 7 1.20E-9
5 SGTC_2367 9070478 5.08 135.4 µM 4.81E-7 cell wall 18 1.86E-7
6 SGTC_448 monastrol 4.94 171.0 µM 9.49E-7 4 3.86E-7
7 SGTC_2099 5377090 4.38 177.4 µM 1.21E-5 12 5.95E-6
8 SGTC_1932 st074806 4.36 40.9 µM 1.32E-5 18 6.53E-6
9 SGTC_877 0771-0151 4.26 33.1 µM 1.97E-5 12 1.00E-5
10 SGTC_2359 9039622 4.25 200.0 µM 2.13E-5 endomembrane recycling 18 1.09E-5
11 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 4.12 3.7 µM 3.57E-5 76 1.90E-5
12 SGTC_880 0833-0487 4.08 87.2 µM 4.12E-5 mitochondrial processes 15 2.22E-5
13 SGTC_1924 st058459 4.08 75.1 µM 4.17E-5 9 2.25E-5
14 SGTC_1929 genistein trimethyl ether 3.84 50.5 µM 1.05E-4 17 6.07E-5
15 SGTC_1018 4259-0013 3.73 28.1 µM 1.63E-4 28 9.71E-5
16 SGTC_656 4090-1979 3.71 187.0 µM 1.76E-4 24 1.05E-4
17 SGTC_3147 9097027 3.52 49.5 µM 3.43E-4 37 2.16E-4
18 SGTC_1725 st036284 3.49 20.6 µM 3.81E-4 50 2.42E-4
19 SGTC_1100 amiodarone 3.44 5.1 µM 4.59E-4 30 2.95E-4
20 SGTC_1727 st034309 3.28 9.9 µM 7.67E-4 79 5.12E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.205 4.68E-33 YNL242W ATG2 hom Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress
0.183 1.04E-26 YGL004C RPN14 hom Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p
0.179 1.27E-25 YOR155C ISN1 hom Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms
0.177 5.10E-25 YDL049C KNH1 hom Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
0.160 9.30E-21 YNL176C_p TDA7_p hom Cell cycle-regulated gene of unknown function, promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele
0.149 3.28E-18 YKR011C YKR011C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress
0.145 2.63E-17 YKL205W LOS1 hom Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm
0.145 3.17E-17 YLR263W RED1 hom Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p
0.145 3.63E-17 YDL029W ARP2 het Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
0.143 9.65E-17 YBR274W CHK1 hom Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase
0.137 1.46E-15 YNL218W MGS1 hom Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress
0.136 2.37E-15 YMR173W DDR48 hom DNA damage-responsive protein; expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS; protein abundance increases in response to DNA replication stress
0.134 6.42E-15 YJL058C BIT61 hom Subunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication
0.132 1.63E-14 YMR145C NDE1 hom Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain
0.127 1.83E-13 YOR300W_d YOR300W_d hom Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7