YLR443W / ECM7

Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR443W / ECM7

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:11.30|P-value:4.28E-30 ID:SGTC_95|Compound:0471-0001|FD-Score:3.25|P-value:5.80E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:3.23|P-value:6.21E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:4.50|P-value:3.47E-6 ID:SGTC_231|Compound:b-estradiol|FD-Score:3.45|P-value:2.80E-4 ID:SGTC_248|Compound:streptozotocin|FD-Score:3.55|P-value:1.93E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:3.54|P-value:2.00E-4 ID:SGTC_560|Compound:2332-1943|FD-Score:3.10|P-value:9.78E-4 ID:SGTC_677|Compound:0929-0063|FD-Score:4.01|P-value:2.97E-5 ID:SGTC_741|Compound:4582-0002|FD-Score:6.09|P-value:5.70E-10 ID:SGTC_869|Compound:0564-0080|FD-Score:-3.29|P-value:5.01E-4 ID:SGTC_874|Compound:0599-0792|FD-Score:4.03|P-value:2.84E-5 ID:SGTC_929|Compound:2805-0021|FD-Score:3.26|P-value:5.48E-4 ID:SGTC_976|Compound:1348-1067|FD-Score:3.82|P-value:6.55E-5 ID:SGTC_1056|Compound:3448-1962|FD-Score:3.29|P-value:5.03E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:3.16|P-value:8.00E-4 ID:SGTC_1168|Compound:k815-0004|FD-Score:3.91|P-value:4.60E-5 ID:SGTC_1177|Compound:0983-0204|FD-Score:3.51|P-value:2.20E-4 ID:SGTC_1183|Compound:1254-0324|FD-Score:-3.38|P-value:3.58E-4 ID:SGTC_1214|Compound:0358-0031|FD-Score:3.94|P-value:4.14E-5 ID:SGTC_1256|Compound:0570-0116|FD-Score:3.35|P-value:4.08E-4 ID:SGTC_1317|Compound:1273-0028|FD-Score:3.32|P-value:4.44E-4 ID:SGTC_1346|Compound:1488-2224|FD-Score:-4.08|P-value:2.29E-5 ID:SGTC_1347|Compound:1493-0251|FD-Score:3.26|P-value:5.58E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:3.98|P-value:3.40E-5 ID:SGTC_1502|Compound:4534-4282|FD-Score:3.42|P-value:3.08E-4 ID:SGTC_1647|Compound:st011722|FD-Score:3.35|P-value:4.03E-4 ID:SGTC_2018|Compound:5133068|FD-Score:3.31|P-value:4.68E-4 ID:SGTC_2051|Compound:5263410|FD-Score:3.46|P-value:2.69E-4 ID:SGTC_2127|Compound:5253358|FD-Score:-3.22|P-value:6.31E-4 ID:SGTC_2324|Compound:6149169|FD-Score:-3.10|P-value:9.73E-4 ID:SGTC_2352|Compound:9027995|FD-Score:-3.33|P-value:4.37E-4 ID:SGTC_2355|Compound:9061122|FD-Score:3.17|P-value:7.67E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:3.17|P-value:7.63E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.20|P-value:6.90E-4 ID:SGTC_2649|Compound:anisomycin|FD-Score:3.56|P-value:1.85E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:9.99|P-value:8.73E-24 ID:SGTC_2692|Compound:st077426|FD-Score:-3.14|P-value:8.29E-4 ID:SGTC_2714|Compound:st079313|FD-Score:3.18|P-value:7.33E-4 ID:SGTC_2942|Compound:9052777|FD-Score:3.34|P-value:4.12E-4 ID:SGTC_2990|Compound:9074105|FD-Score:3.36|P-value:3.90E-4 ID:SGTC_3037|Compound:9091026|FD-Score:3.47|P-value:2.57E-4 ID:SGTC_3088|Compound:9117578|FD-Score:3.10|P-value:9.71E-4 ID:SGTC_3132|Compound:9126706|FD-Score:-3.56|P-value:1.82E-4 ID:SGTC_3134|Compound:9127454|FD-Score:4.18|P-value:1.47E-5 ID:SGTC_3205|Compound:9113483|FD-Score:-3.78|P-value:7.87E-5 ID:SGTC_3299|Compound:9121982|FD-Score:4.20|P-value:1.34E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:11.30|P-value:4.28E-30 ID:SGTC_95|Compound:0471-0001|FD-Score:3.25|P-value:5.80E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:3.23|P-value:6.21E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:4.50|P-value:3.47E-6 ID:SGTC_231|Compound:b-estradiol|FD-Score:3.45|P-value:2.80E-4 ID:SGTC_248|Compound:streptozotocin|FD-Score:3.55|P-value:1.93E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:3.54|P-value:2.00E-4 ID:SGTC_560|Compound:2332-1943|FD-Score:3.10|P-value:9.78E-4 ID:SGTC_677|Compound:0929-0063|FD-Score:4.01|P-value:2.97E-5 ID:SGTC_741|Compound:4582-0002|FD-Score:6.09|P-value:5.70E-10 ID:SGTC_869|Compound:0564-0080|FD-Score:-3.29|P-value:5.01E-4 ID:SGTC_874|Compound:0599-0792|FD-Score:4.03|P-value:2.84E-5 ID:SGTC_929|Compound:2805-0021|FD-Score:3.26|P-value:5.48E-4 ID:SGTC_976|Compound:1348-1067|FD-Score:3.82|P-value:6.55E-5 ID:SGTC_1056|Compound:3448-1962|FD-Score:3.29|P-value:5.03E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:3.16|P-value:8.00E-4 ID:SGTC_1168|Compound:k815-0004|FD-Score:3.91|P-value:4.60E-5 ID:SGTC_1177|Compound:0983-0204|FD-Score:3.51|P-value:2.20E-4 ID:SGTC_1183|Compound:1254-0324|FD-Score:-3.38|P-value:3.58E-4 ID:SGTC_1214|Compound:0358-0031|FD-Score:3.94|P-value:4.14E-5 ID:SGTC_1256|Compound:0570-0116|FD-Score:3.35|P-value:4.08E-4 ID:SGTC_1317|Compound:1273-0028|FD-Score:3.32|P-value:4.44E-4 ID:SGTC_1346|Compound:1488-2224|FD-Score:-4.08|P-value:2.29E-5 ID:SGTC_1347|Compound:1493-0251|FD-Score:3.26|P-value:5.58E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:3.98|P-value:3.40E-5 ID:SGTC_1502|Compound:4534-4282|FD-Score:3.42|P-value:3.08E-4 ID:SGTC_1647|Compound:st011722|FD-Score:3.35|P-value:4.03E-4 ID:SGTC_2018|Compound:5133068|FD-Score:3.31|P-value:4.68E-4 ID:SGTC_2051|Compound:5263410|FD-Score:3.46|P-value:2.69E-4 ID:SGTC_2127|Compound:5253358|FD-Score:-3.22|P-value:6.31E-4 ID:SGTC_2324|Compound:6149169|FD-Score:-3.10|P-value:9.73E-4 ID:SGTC_2352|Compound:9027995|FD-Score:-3.33|P-value:4.37E-4 ID:SGTC_2355|Compound:9061122|FD-Score:3.17|P-value:7.67E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:3.17|P-value:7.63E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.20|P-value:6.90E-4 ID:SGTC_2649|Compound:anisomycin|FD-Score:3.56|P-value:1.85E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:9.99|P-value:8.73E-24 ID:SGTC_2692|Compound:st077426|FD-Score:-3.14|P-value:8.29E-4 ID:SGTC_2714|Compound:st079313|FD-Score:3.18|P-value:7.33E-4 ID:SGTC_2942|Compound:9052777|FD-Score:3.34|P-value:4.12E-4 ID:SGTC_2990|Compound:9074105|FD-Score:3.36|P-value:3.90E-4 ID:SGTC_3037|Compound:9091026|FD-Score:3.47|P-value:2.57E-4 ID:SGTC_3088|Compound:9117578|FD-Score:3.10|P-value:9.71E-4 ID:SGTC_3132|Compound:9126706|FD-Score:-3.56|P-value:1.82E-4 ID:SGTC_3134|Compound:9127454|FD-Score:4.18|P-value:1.47E-5 ID:SGTC_3205|Compound:9113483|FD-Score:-3.78|P-value:7.87E-5 ID:SGTC_3299|Compound:9121982|FD-Score:4.20|P-value:1.34E-5

Top fitness defect scores for YLR443W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_58 0957-0393 11.30 9.0 µM 4.28E-30 cell wall 60 4.36E-30
2 SGTC_2688 kanamycin b 9.99 96.7 µM 8.73E-24 calcium & mitochondrial duress 26 8.84E-24
3 SGTC_741 4582-0002 6.09 126.0 µM 5.70E-10 1 5.73E-10
4 SGTC_138 0850-0259 4.50 40.9 µM 3.47E-6 cell wall 166 3.47E-6
5 SGTC_3299 9121982 4.20 43.1 µM 1.34E-5 20 1.35E-5
6 SGTC_3134 9127454 4.18 49.5 µM 1.47E-5 9 1.48E-5
7 SGTC_874 0599-0792 4.03 117.0 µM 2.84E-5 24 2.85E-5
8 SGTC_677 0929-0063 4.01 136.0 µM 2.97E-5 16 2.98E-5
9 SGTC_1378 2093-0007 3.98 380.0 nM 3.40E-5 21 3.40E-5
10 SGTC_1214 0358-0031 3.94 63.7 µM 4.14E-5 20 4.15E-5
11 SGTC_1168 k815-0004 3.91 80.5 µM 4.60E-5 DNA damage response 19 4.61E-5
12 SGTC_976 1348-1067 3.82 47.6 µM 6.55E-5 60S ribosome export 35 6.56E-5
13 SGTC_2649 anisomycin 3.56 9.6 µM 1.85E-4 16 1.85E-4
14 SGTC_248 streptozotocin 3.55 5.2 mM 1.93E-4 DNA damage response 30 1.93E-4
15 SGTC_310 1273-0058 3.54 1.1 µM 2.00E-4 mitochondrial stress 283 2.01E-4
16 SGTC_1177 0983-0204 3.51 23.6 µM 2.20E-4 24 2.20E-4
17 SGTC_3037 9091026 3.47 49.5 µM 2.57E-4 NEO1-PIK1 153 2.57E-4
18 SGTC_2051 5263410 3.46 176.0 µM 2.69E-4 29 2.69E-4
19 SGTC_231 b-estradiol 3.45 524.0 µM 2.80E-4 13 2.80E-4
20 SGTC_1502 4534-4282 3.42 27.7 µM 3.08E-4 27 3.08E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.341 6.57E-92 YGR217W CCH1 hom Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together
0.284 2.70E-63 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.284 4.05E-63 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.270 3.20E-57 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.266 1.91E-55 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.263 2.87E-54 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.245 5.15E-47 YML016C PPZ1 hom Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance
0.243 1.96E-46 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.243 1.90E-46 YLR150W STM1 hom Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
0.238 2.53E-44 YIL148W RPL40A hom Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.233 1.04E-42 YML021C UNG1 hom Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus
0.233 1.29E-42 YDR335W MSN5 hom Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm
0.228 7.01E-41 YBR291C CTP1 hom Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family
0.227 1.86E-40 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.227 2.25E-40 YDL213C NOP6 hom rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes