S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA
Zygosity: Homozygous strain
fixedexpanded
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Top fitness defect scores for YML005W deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Correlation | pval | ORF | Gene | Zygosity | Description |
---|---|---|---|---|---|
0.139 | 7.18E-16 | YDR452W | PPN1 | hom | Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress |
0.107 | 5.79E-10 | YDR489W | SLD5 | het | Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery |
0.104 | 1.57E-9 | YLR084C | RAX2 | hom | N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p |
0.100 | 6.17E-9 | YMR040W | YET2 | hom | Protein of unknown function that may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein |
0.096 | 2.45E-8 | YCL001W-B_p | YCL001W-B_p | hom | Putative protein of unknown function; present in a region duplicated between chromosomes XIV and III; YCL001W-B has a paralog, DOM34, that arose from the whole genome duplication |
0.088 | 3.30E-7 | YDR101C | ARX1 | hom | Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex |
0.084 | 1.17E-6 | YIR034C | LYS1 | hom | Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity |
0.082 | 1.81E-6 | YOR354C | MSC6 | hom | Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
0.081 | 2.68E-6 | YDR390C | UBA2 | het | Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability |
0.074 | 1.60E-5 | YOR176W | HEM15 | het | Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway |
0.074 | 1.84E-5 | YLR062C_d | BUD28_d | hom | Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay |
0.074 | 1.95E-5 | YGR126W_p | YGR126W_p | hom | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS |
0.073 | 2.08E-5 | YDR231C | COX20 | hom | Mitochondrial inner membrane protein, required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase |
0.073 | 2.18E-5 | YDL107W | MSS2 | hom | Peripherally bound inner membrane protein of the mitochondrial matrix involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p |
0.073 | 2.20E-5 | YER178W | PDA1 | hom | E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose |