YML005W / TRM12

S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA

Zygosity: Homozygous strain
fixedexpanded
Profile for YML005W / TRM12

Click on Significant Values for Screen Details ID:SGTC_56|Compound:0957-0365|FD-Score:-5.52|P-value:1.08E-8 ID:SGTC_57|Compound:miconazole|FD-Score:-4.05|P-value:1.84E-5 ID:SGTC_134|Compound:0269-0018|FD-Score:3.25|P-value:7.74E-4 ID:SGTC_196|Compound:k015-0023|FD-Score:-3.06|P-value:8.57E-4 ID:SGTC_199|Compound:4063-0024|FD-Score:-4.24|P-value:8.01E-6 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:-3.23|P-value:4.76E-4 ID:SGTC_246|Compound:cycloheximide|FD-Score:3.39|P-value:4.74E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:3.56|P-value:2.52E-4 ID:SGTC_355|Compound:1311-2252|FD-Score:-3.55|P-value:1.41E-4 ID:SGTC_395|Compound:anisomycin|FD-Score:-3.42|P-value:2.37E-4 ID:SGTC_404|Compound:fumagillin|FD-Score:4.06|P-value:3.47E-5 ID:SGTC_416|Compound:estradiol valerate|FD-Score:-3.25|P-value:4.41E-4 ID:SGTC_449|Compound:xestospongin c|FD-Score:-3.38|P-value:2.69E-4 ID:SGTC_450|Compound:aminopyrene|FD-Score:3.48|P-value:3.43E-4 ID:SGTC_753|Compound:1322-0200|FD-Score:3.61|P-value:2.06E-4 ID:SGTC_799|Compound:1310-0557|FD-Score:3.39|P-value:4.72E-4 ID:SGTC_934|Compound:2950-0684|FD-Score:-3.38|P-value:2.71E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:3.29|P-value:6.72E-4 ID:SGTC_1116|Compound:jasmonic acid|FD-Score:-3.44|P-value:2.15E-4 ID:SGTC_1149|Compound:3909-7961|FD-Score:3.25|P-value:7.78E-4 ID:SGTC_1343|Compound:1487-0095|FD-Score:-3.11|P-value:7.23E-4 ID:SGTC_1508|Compound:1080-0625|FD-Score:-4.05|P-value:1.80E-5 ID:SGTC_1782|Compound:st039423|FD-Score:4.13|P-value:2.64E-5 ID:SGTC_2061|Compound:5245884|FD-Score:-4.25|P-value:7.71E-6 ID:SGTC_2065|Compound:5221596|FD-Score:-5.05|P-value:1.51E-7 ID:SGTC_2076|Compound:5212012|FD-Score:-3.29|P-value:3.81E-4 ID:SGTC_2427|Compound:5545055|FD-Score:3.19|P-value:9.60E-4 ID:SGTC_2482|Compound:5981709|FD-Score:3.21|P-value:8.91E-4 ID:SGTC_2484|Compound:5809818|FD-Score:3.27|P-value:7.23E-4 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:-4.48|P-value:2.54E-6 ID:SGTC_2550|Compound:chrysin dimethyl ether|FD-Score:-3.38|P-value:2.71E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:3.18|P-value:9.92E-4 ID:SGTC_2935|Compound:9038016|FD-Score:-3.22|P-value:4.96E-4 ID:SGTC_3054|Compound:9094397|FD-Score:3.27|P-value:7.24E-4 ID:SGTC_56|Compound:0957-0365|FD-Score:-5.52|P-value:1.08E-8 ID:SGTC_57|Compound:miconazole|FD-Score:-4.05|P-value:1.84E-5 ID:SGTC_134|Compound:0269-0018|FD-Score:3.25|P-value:7.74E-4 ID:SGTC_196|Compound:k015-0023|FD-Score:-3.06|P-value:8.57E-4 ID:SGTC_199|Compound:4063-0024|FD-Score:-4.24|P-value:8.01E-6 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:-3.23|P-value:4.76E-4 ID:SGTC_246|Compound:cycloheximide|FD-Score:3.39|P-value:4.74E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:3.56|P-value:2.52E-4 ID:SGTC_355|Compound:1311-2252|FD-Score:-3.55|P-value:1.41E-4 ID:SGTC_395|Compound:anisomycin|FD-Score:-3.42|P-value:2.37E-4 ID:SGTC_404|Compound:fumagillin|FD-Score:4.06|P-value:3.47E-5 ID:SGTC_416|Compound:estradiol valerate|FD-Score:-3.25|P-value:4.41E-4 ID:SGTC_449|Compound:xestospongin c|FD-Score:-3.38|P-value:2.69E-4 ID:SGTC_450|Compound:aminopyrene|FD-Score:3.48|P-value:3.43E-4 ID:SGTC_753|Compound:1322-0200|FD-Score:3.61|P-value:2.06E-4 ID:SGTC_799|Compound:1310-0557|FD-Score:3.39|P-value:4.72E-4 ID:SGTC_934|Compound:2950-0684|FD-Score:-3.38|P-value:2.71E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:3.29|P-value:6.72E-4 ID:SGTC_1116|Compound:jasmonic acid|FD-Score:-3.44|P-value:2.15E-4 ID:SGTC_1149|Compound:3909-7961|FD-Score:3.25|P-value:7.78E-4 ID:SGTC_1343|Compound:1487-0095|FD-Score:-3.11|P-value:7.23E-4 ID:SGTC_1508|Compound:1080-0625|FD-Score:-4.05|P-value:1.80E-5 ID:SGTC_1782|Compound:st039423|FD-Score:4.13|P-value:2.64E-5 ID:SGTC_2061|Compound:5245884|FD-Score:-4.25|P-value:7.71E-6 ID:SGTC_2065|Compound:5221596|FD-Score:-5.05|P-value:1.51E-7 ID:SGTC_2076|Compound:5212012|FD-Score:-3.29|P-value:3.81E-4 ID:SGTC_2427|Compound:5545055|FD-Score:3.19|P-value:9.60E-4 ID:SGTC_2482|Compound:5981709|FD-Score:3.21|P-value:8.91E-4 ID:SGTC_2484|Compound:5809818|FD-Score:3.27|P-value:7.23E-4 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:-4.48|P-value:2.54E-6 ID:SGTC_2550|Compound:chrysin dimethyl ether|FD-Score:-3.38|P-value:2.71E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:3.18|P-value:9.92E-4 ID:SGTC_2935|Compound:9038016|FD-Score:-3.22|P-value:4.96E-4 ID:SGTC_3054|Compound:9094397|FD-Score:3.27|P-value:7.24E-4

Top fitness defect scores for YML005W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1782 st039423 4.13 80.6 µM 2.64E-5 18 1.84E-5
2 SGTC_404 fumagillin 4.06 50.0 µM 3.47E-5 17 2.44E-5
3 SGTC_753 1322-0200 3.61 289.0 µM 2.06E-4 31 1.50E-4
4 SGTC_353 1988-1175 3.56 58.3 µM 2.52E-4 unfolded protein response 32 1.84E-4
5 SGTC_450 aminopyrene 3.48 156.0 nM 3.43E-4 55 2.53E-4
6 SGTC_799 1310-0557 3.39 151.0 µM 4.72E-4 superoxide 43 3.50E-4
7 SGTC_246 cycloheximide 3.39 667.0 µM 4.74E-4 28 3.52E-4
8 SGTC_1108 betulinic acid 3.29 729.1 nM 6.72E-4 31 5.03E-4
9 SGTC_2484 5809818 3.27 12.0 µM 7.23E-4 34 5.42E-4
10 SGTC_3054 9094397 3.27 49.5 µM 7.24E-4 fatty acid desaturase (OLE1) 63 5.43E-4
11 SGTC_134 0269-0018 3.25 6.9 µM 7.74E-4 174 5.81E-4
12 SGTC_1149 3909-7961 3.25 23.3 µM 7.78E-4 57 5.84E-4
13 SGTC_2482 5981709 3.21 142.7 µM 8.91E-4 PDR1 70 6.71E-4
14 SGTC_2427 5545055 3.19 42.8 µM 9.60E-4 43 7.24E-4
15 SGTC_2760 etidronate 3.18 98.0 µM 9.92E-4 60 7.48E-4
16 SGTC_929 2805-0021 3.17 398.0 µM 0.00102 38 7.73E-4
17 SGTC_1110 3-hydroxy-3'-methoxyflavone 3.16 1.2 µM 0.00104 iron homeostasis 82 7.84E-4
18 SGTC_54 0159-0055 3.16 9.0 µM 0.00105 ERG2 68 7.94E-4
19 SGTC_2343 7990716 3.11 14.8 µM 0.00123 45 9.31E-4
20 SGTC_1065 prochlorperazine maleate 3.09 2.5 µM 0.00130 43 9.85E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.139 7.18E-16 YDR452W PPN1 hom Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress
0.107 5.79E-10 YDR489W SLD5 het Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
0.104 1.57E-9 YLR084C RAX2 hom N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p
0.100 6.17E-9 YMR040W YET2 hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein
0.096 2.45E-8 YCL001W-B_p YCL001W-B_p hom Putative protein of unknown function; present in a region duplicated between chromosomes XIV and III; YCL001W-B has a paralog, DOM34, that arose from the whole genome duplication
0.088 3.30E-7 YDR101C ARX1 hom Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex
0.084 1.17E-6 YIR034C LYS1 hom Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity
0.082 1.81E-6 YOR354C MSC6 hom Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.081 2.68E-6 YDR390C UBA2 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.074 1.60E-5 YOR176W HEM15 het Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway
0.074 1.84E-5 YLR062C_d BUD28_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay
0.074 1.95E-5 YGR126W_p YGR126W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
0.073 2.08E-5 YDR231C COX20 hom Mitochondrial inner membrane protein, required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase
0.073 2.18E-5 YDL107W MSS2 hom Peripherally bound inner membrane protein of the mitochondrial matrix involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p
0.073 2.20E-5 YER178W PDA1 hom E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose