YML095C / RAD10

Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein

Zygosity: Homozygous strain
fixedexpanded
Profile for YML095C / RAD10

Click on Significant Values for Screen Details ID:SGTC_24|Compound:0417-1663|FD-Score:3.11|P-value:3.41E-4 ID:SGTC_84|Compound:0443-0269|FD-Score:2.92|P-value:7.10E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:3.14|P-value:2.96E-4 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:3.09|P-value:3.60E-4 ID:SGTC_247|Compound:mechlorethamine|FD-Score:7.03|P-value:6.77E-15 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:34.10|P-value:1.16E-306 ID:SGTC_280|Compound:3448-3607|FD-Score:4.18|P-value:2.34E-6 ID:SGTC_594|Compound:1487-1584|FD-Score:3.10|P-value:3.50E-4 ID:SGTC_620|Compound:0109-0052|FD-Score:3.38|P-value:1.08E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:3.03|P-value:4.57E-4 ID:SGTC_816|Compound:0325-0417|FD-Score:3.38|P-value:1.10E-4 ID:SGTC_826|Compound:1683-5028|FD-Score:3.06|P-value:4.18E-4 ID:SGTC_940|Compound:3232-1287|FD-Score:3.31|P-value:1.51E-4 ID:SGTC_1051|Compound:3253-3903|FD-Score:-3.92|P-value:7.50E-6 ID:SGTC_1052|Compound:3253-3998|FD-Score:3.36|P-value:1.17E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:4.72|P-value:1.17E-7 ID:SGTC_1109|Compound:tnp00067|FD-Score:-2.94|P-value:5.84E-4 ID:SGTC_1293|Compound:1000-1278|FD-Score:3.02|P-value:4.81E-4 ID:SGTC_1307|Compound:1187-1599|FD-Score:-3.69|P-value:2.29E-5 ID:SGTC_1308|Compound:1187-1617|FD-Score:-2.84|P-value:8.58E-4 ID:SGTC_1351|Compound:1496-0003|FD-Score:2.93|P-value:6.91E-4 ID:SGTC_1651|Compound:st011928|FD-Score:-3.10|P-value:3.06E-4 ID:SGTC_1668|Compound:st014914|FD-Score:2.90|P-value:7.59E-4 ID:SGTC_1669|Compound:st015249|FD-Score:2.96|P-value:6.22E-4 ID:SGTC_1672|Compound:st016598|FD-Score:-3.15|P-value:2.55E-4 ID:SGTC_1795|Compound:st049545|FD-Score:3.66|P-value:3.09E-5 ID:SGTC_1910|Compound:st062352|FD-Score:-3.54|P-value:4.78E-5 ID:SGTC_1944|Compound:st076371|FD-Score:-2.84|P-value:8.68E-4 ID:SGTC_1970|Compound:st070122|FD-Score:3.44|P-value:8.34E-5 ID:SGTC_2005|Compound:4024620|FD-Score:-3.03|P-value:4.09E-4 ID:SGTC_2068|Compound:5226020|FD-Score:-3.20|P-value:2.05E-4 ID:SGTC_2430|Compound:5571206|FD-Score:2.89|P-value:7.85E-4 ID:SGTC_2561|Compound:acetosyringone|FD-Score:-3.03|P-value:4.06E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:2.94|P-value:6.51E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:2.96|P-value:6.12E-4 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:-3.39|P-value:9.29E-5 ID:SGTC_2677|Compound:mitomycin C|FD-Score:6.99|P-value:8.84E-15 ID:SGTC_2779|Compound:methotrexate|FD-Score:3.24|P-value:2.02E-4 ID:SGTC_2798|Compound:7813637|FD-Score:-2.85|P-value:8.25E-4 ID:SGTC_2947|Compound:9072639|FD-Score:-3.77|P-value:1.60E-5 ID:SGTC_2986|Compound:9022445|FD-Score:2.86|P-value:8.77E-4 ID:SGTC_3016|Compound:9080866|FD-Score:-2.91|P-value:6.52E-4 ID:SGTC_3195|Compound:9113082|FD-Score:6.40|P-value:1.14E-12 ID:SGTC_3265|Compound:9137266|FD-Score:2.87|P-value:8.74E-4 ID:SGTC_3294|Compound:9122953|FD-Score:6.24|P-value:4.01E-12 ID:SGTC_24|Compound:0417-1663|FD-Score:3.11|P-value:3.41E-4 ID:SGTC_84|Compound:0443-0269|FD-Score:2.92|P-value:7.10E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:3.14|P-value:2.96E-4 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:3.09|P-value:3.60E-4 ID:SGTC_247|Compound:mechlorethamine|FD-Score:7.03|P-value:6.77E-15 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:34.10|P-value:1.16E-306 ID:SGTC_280|Compound:3448-3607|FD-Score:4.18|P-value:2.34E-6 ID:SGTC_594|Compound:1487-1584|FD-Score:3.10|P-value:3.50E-4 ID:SGTC_620|Compound:0109-0052|FD-Score:3.38|P-value:1.08E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:3.03|P-value:4.57E-4 ID:SGTC_816|Compound:0325-0417|FD-Score:3.38|P-value:1.10E-4 ID:SGTC_826|Compound:1683-5028|FD-Score:3.06|P-value:4.18E-4 ID:SGTC_940|Compound:3232-1287|FD-Score:3.31|P-value:1.51E-4 ID:SGTC_1051|Compound:3253-3903|FD-Score:-3.92|P-value:7.50E-6 ID:SGTC_1052|Compound:3253-3998|FD-Score:3.36|P-value:1.17E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:4.72|P-value:1.17E-7 ID:SGTC_1109|Compound:tnp00067|FD-Score:-2.94|P-value:5.84E-4 ID:SGTC_1293|Compound:1000-1278|FD-Score:3.02|P-value:4.81E-4 ID:SGTC_1307|Compound:1187-1599|FD-Score:-3.69|P-value:2.29E-5 ID:SGTC_1308|Compound:1187-1617|FD-Score:-2.84|P-value:8.58E-4 ID:SGTC_1351|Compound:1496-0003|FD-Score:2.93|P-value:6.91E-4 ID:SGTC_1651|Compound:st011928|FD-Score:-3.10|P-value:3.06E-4 ID:SGTC_1668|Compound:st014914|FD-Score:2.90|P-value:7.59E-4 ID:SGTC_1669|Compound:st015249|FD-Score:2.96|P-value:6.22E-4 ID:SGTC_1672|Compound:st016598|FD-Score:-3.15|P-value:2.55E-4 ID:SGTC_1795|Compound:st049545|FD-Score:3.66|P-value:3.09E-5 ID:SGTC_1910|Compound:st062352|FD-Score:-3.54|P-value:4.78E-5 ID:SGTC_1944|Compound:st076371|FD-Score:-2.84|P-value:8.68E-4 ID:SGTC_1970|Compound:st070122|FD-Score:3.44|P-value:8.34E-5 ID:SGTC_2005|Compound:4024620|FD-Score:-3.03|P-value:4.09E-4 ID:SGTC_2068|Compound:5226020|FD-Score:-3.20|P-value:2.05E-4 ID:SGTC_2430|Compound:5571206|FD-Score:2.89|P-value:7.85E-4 ID:SGTC_2561|Compound:acetosyringone|FD-Score:-3.03|P-value:4.06E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:2.94|P-value:6.51E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:2.96|P-value:6.12E-4 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:-3.39|P-value:9.29E-5 ID:SGTC_2677|Compound:mitomycin C|FD-Score:6.99|P-value:8.84E-15 ID:SGTC_2779|Compound:methotrexate|FD-Score:3.24|P-value:2.02E-4 ID:SGTC_2798|Compound:7813637|FD-Score:-2.85|P-value:8.25E-4 ID:SGTC_2947|Compound:9072639|FD-Score:-3.77|P-value:1.60E-5 ID:SGTC_2986|Compound:9022445|FD-Score:2.86|P-value:8.77E-4 ID:SGTC_3016|Compound:9080866|FD-Score:-2.91|P-value:6.52E-4 ID:SGTC_3195|Compound:9113082|FD-Score:6.40|P-value:1.14E-12 ID:SGTC_3265|Compound:9137266|FD-Score:2.87|P-value:8.74E-4 ID:SGTC_3294|Compound:9122953|FD-Score:6.24|P-value:4.01E-12

Top fitness defect scores for YML095C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_267 4-Nitroquinoline-1-oxide (4-NQO) 34.10 44.4 nM 1.16E-306 DNA damage response 2 9.06E-255
2 SGTC_247 mechlorethamine 7.03 29.7 µM 6.77E-15 DNA damage response 3 1.07E-12
3 SGTC_2677 mitomycin C 6.99 100.0 µM 8.84E-15 DNA damage response 3 1.33E-12
4 SGTC_3195 9113082 6.40 49.5 µM 1.14E-12 2 7.62E-11
5 SGTC_3294 9122953 6.24 26.3 µM 4.01E-12 tubulin folding & SWR complex 8 2.18E-10
6 SGTC_1060 3448-7350 4.72 82.0 µM 1.17E-7 Golgi 21 1.17E-6
7 SGTC_280 3448-3607 4.18 3.0 µM 2.34E-6 60S ribosome export 6 1.43E-5
8 SGTC_1795 st049545 3.66 62.3 µM 3.09E-5 TSC3-RPN4 17 1.25E-4
9 SGTC_1970 st070122 3.44 35.8 µM 8.34E-5 TSC3-RPN4 40 2.88E-4
10 SGTC_620 0109-0052 3.38 432.0 µM 1.08E-4 36 3.58E-4
11 SGTC_816 0325-0417 3.38 81.3 µM 1.10E-4 25 3.62E-4
12 SGTC_1052 3253-3998 3.36 198.0 µM 1.17E-4 38 3.83E-4
13 SGTC_940 3232-1287 3.31 54.8 µM 1.51E-4 NEO1 63 4.73E-4
14 SGTC_2779 methotrexate 3.24 400.0 µM 2.02E-4 53 6.05E-4
15 SGTC_138 0850-0259 3.14 40.9 µM 2.96E-4 cell wall 264 8.36E-4
16 SGTC_24 0417-1663 3.11 219.0 µM 3.41E-4 50 9.41E-4
17 SGTC_594 1487-1584 3.10 10.8 µM 3.50E-4 53 9.62E-4
18 SGTC_239 5hr heat shock (37°C) + mitomycin C 3.09 819.4 µM 3.60E-4 heat shock/prefoldin 69 9.87E-4
19 SGTC_826 1683-5028 3.06 118.0 µM 4.18E-4 45 0.00112
20 SGTC_805 3852-0218 3.03 479.0 µM 4.57E-4 ERAD & cell cycle 117 0.00121

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.736 0 YMR201C RAD14 hom Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein
0.724 0 YGR258C RAD2 hom Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein
0.626 0 YPL022W RAD1 hom Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein
0.587 2.34E-309 YER162C RAD4 hom Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins
0.442 1.69E-160 YDR092W UBC13 hom Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus
0.440 3.58E-159 YEL037C RAD23 hom Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover
0.369 1.14E-108 YGL087C MMS2 hom Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress
0.295 2.57E-68 YLR032W RAD5 hom DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
0.261 1.94E-53 YCR066W RAD18 hom E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
0.260 6.54E-53 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.253 2.74E-50 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.221 3.17E-38 YIR002C MPH1 hom 3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases
0.199 2.07E-31 YBR099C_d YBR099C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
0.195 5.84E-30 YJL092W SRS2 hom DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability
0.170 3.08E-23 YFL047W RGD2 hom GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress