Protein of unknown function; structure defines a new subfamily of the split beta-alpha-beta sandwiches; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YML108W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress

Zygosity: Homozygous strain
Profile for YML108W / YML108W

Click on Significant Values for Screen Details

Top fitness defect scores for YML108W deletion by condition

RankScreen IDConditionFD ScoreConc
1 SGTC_822 1487-0950 3.88 154.0 µM 1.11E-5 14 5.24E-5
2 SGTC_2590 3,4',5',6',7'-pentamethoxyflavone 3.47 100.0 µM 7.35E-5 60S ribosome export 40 2.61E-4
3 SGTC_2400 5203494 3.42 178.7 µM 9.21E-5 RSC complex & mRNA processing 36 3.16E-4
4 SGTC_2591 skatole 3.23 100.0 µM 2.03E-4 49 6.19E-4
5 SGTC_357 1325-0123 3.21 420.0 µM 2.18E-4 27 6.55E-4
6 SGTC_285 1486-1325 3.15 46.0 µM 2.83E-4 48 8.19E-4
7 SGTC_2156 5649887 3.12 115.0 µM 3.14E-4 RPP1 & pyrimidine depletion 44 8.94E-4
8 SGTC_368 fentichlor 3.12 4.6 µM 3.15E-4 55 8.97E-4
9 SGTC_2129 girgensonine 2.99 121.0 µM 5.23E-4 heme biosynthesis & mitochondrial translocase 83 0.00138
10 SGTC_281 1672-3440 2.91 14.9 µM 7.15E-4 Golgi 33 0.00180
11 SGTC_292 k038-0015 2.87 474.0 µM 8.20E-4 40 0.00202
12 SGTC_1489 4065-0444 2.85 182.0 µM 9.08E-4 68 0.00220
13 SGTC_57 miconazole 2.85 162.5 nM 9.13E-4 ergosterol depletion effects on membrane 68 0.00221
14 SGTC_2722 naftopidil 2.80 47.2 µM 0.00108 56 0.00256
15 SGTC_63 0099-0242 2.78 26.7 µM 0.00117 mitochondrial stress 177 0.00274
16 SGTC_762 1165-0429 2.77 115.0 µM 0.00119 TSC3-RPN4 83 0.00277
17 SGTC_1746 st038117 2.77 42.0 µM 0.00120 101 0.00279
18 SGTC_1973 st070275 2.73 50.2 µM 0.00138 64 0.00314
19 SGTC_1441 4106-0040 2.73 13.0 µM 0.00140 59 0.00319
20 SGTC_1422 4031-0024 2.69 15.5 µM 0.00159 iron homeostasis 71 0.00355

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.196 2.05E-30 YIL042C PKP1 hom Mitochondrial protein kinase involved in negative regulation of pyruvate dehydrogenase complex activity by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp2p and phosphatases Ptc5p and Ptc6p
0.136 3.26E-15 YLR367W RPS22B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication
0.132 1.85E-14 YBL002W HTB2 hom Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation
0.116 1.54E-11 YGL067W NPY1 hom NADH diphosphatase (pyrophosphatase), hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes; nudix hydrolase family member
0.115 2.12E-11 YOR297C TIM18 hom Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex
0.113 4.46E-11 YKL052C ASK1 het Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress
0.109 2.82E-10 YDL037C BSC1 hom Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression
0.107 5.11E-10 YLR396C VPS33 hom ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole
0.106 7.54E-10 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.102 2.81E-9 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.100 6.14E-9 YPR126C_d YPR126C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.098 1.26E-8 YCL026C-A FRM2 hom Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin
0.097 1.63E-8 YGR077C PEX8 hom Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.097 2.15E-8 YDR312W SSF2 hom Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication
0.095 3.44E-8 YDL176W YDL176W hom Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene