YML113W / DAT1

DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability

Zygosity: Homozygous strain
fixedexpanded
Profile for YML113W / DAT1

Click on Significant Values for Screen Details ID:SGTC_261|Compound:5-fluorouridine|FD-Score:-3.34|P-value:2.08E-4 ID:SGTC_271|Compound:rotenone|FD-Score:-3.22|P-value:3.40E-4 ID:SGTC_273|Compound:hydroxyurea|FD-Score:-3.17|P-value:4.04E-4 ID:SGTC_277|Compound:nystatin|FD-Score:3.23|P-value:3.00E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:3.02|P-value:6.84E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:7.04|P-value:4.23E-14 ID:SGTC_799|Compound:1310-0557|FD-Score:3.84|P-value:2.32E-5 ID:SGTC_863|Compound:0466-0191|FD-Score:4.79|P-value:1.95E-7 ID:SGTC_1226|Compound:3909-7979|FD-Score:3.77|P-value:3.25E-5 ID:SGTC_1235|Compound:benzylparaben|FD-Score:2.92|P-value:9.65E-4 ID:SGTC_1237|Compound:0330-0133|FD-Score:-3.73|P-value:4.06E-5 ID:SGTC_1244|Compound:0416-0015|FD-Score:3.15|P-value:4.12E-4 ID:SGTC_1336|Compound:1486-0901|FD-Score:-3.83|P-value:2.60E-5 ID:SGTC_1376|Compound:2910-0861|FD-Score:3.20|P-value:3.37E-4 ID:SGTC_1406|Compound:3909-7906|FD-Score:-3.48|P-value:1.20E-4 ID:SGTC_1440|Compound:4100-1550|FD-Score:-3.74|P-value:3.95E-5 ID:SGTC_1512|Compound:0259-0544|FD-Score:3.33|P-value:2.04E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-3.16|P-value:4.18E-4 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:2.97|P-value:8.07E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-3.96|P-value:1.46E-5 ID:SGTC_1848|Compound:st056003|FD-Score:-3.61|P-value:6.77E-5 ID:SGTC_1988|Compound:st071872|FD-Score:4.23|P-value:3.60E-6 ID:SGTC_2350|Compound:9023895|FD-Score:-3.60|P-value:7.26E-5 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:-3.14|P-value:4.54E-4 ID:SGTC_2537|Compound:larixol|FD-Score:3.39|P-value:1.60E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:-3.03|P-value:6.86E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:2.93|P-value:9.50E-4 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:4.17|P-value:4.87E-6 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:-3.32|P-value:2.28E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:3.83|P-value:2.47E-5 ID:SGTC_2930|Compound:9013230|FD-Score:4.06|P-value:8.51E-6 ID:SGTC_2937|Compound:9044753|FD-Score:-4.19|P-value:4.87E-6 ID:SGTC_3224|Compound:9130643|FD-Score:4.40|P-value:1.53E-6 ID:SGTC_3319|Compound:9138450|FD-Score:-3.25|P-value:3.02E-4 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:-3.34|P-value:2.08E-4 ID:SGTC_271|Compound:rotenone|FD-Score:-3.22|P-value:3.40E-4 ID:SGTC_273|Compound:hydroxyurea|FD-Score:-3.17|P-value:4.04E-4 ID:SGTC_277|Compound:nystatin|FD-Score:3.23|P-value:3.00E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:3.02|P-value:6.84E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:7.04|P-value:4.23E-14 ID:SGTC_799|Compound:1310-0557|FD-Score:3.84|P-value:2.32E-5 ID:SGTC_863|Compound:0466-0191|FD-Score:4.79|P-value:1.95E-7 ID:SGTC_1226|Compound:3909-7979|FD-Score:3.77|P-value:3.25E-5 ID:SGTC_1235|Compound:benzylparaben|FD-Score:2.92|P-value:9.65E-4 ID:SGTC_1237|Compound:0330-0133|FD-Score:-3.73|P-value:4.06E-5 ID:SGTC_1244|Compound:0416-0015|FD-Score:3.15|P-value:4.12E-4 ID:SGTC_1336|Compound:1486-0901|FD-Score:-3.83|P-value:2.60E-5 ID:SGTC_1376|Compound:2910-0861|FD-Score:3.20|P-value:3.37E-4 ID:SGTC_1406|Compound:3909-7906|FD-Score:-3.48|P-value:1.20E-4 ID:SGTC_1440|Compound:4100-1550|FD-Score:-3.74|P-value:3.95E-5 ID:SGTC_1512|Compound:0259-0544|FD-Score:3.33|P-value:2.04E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-3.16|P-value:4.18E-4 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:2.97|P-value:8.07E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-3.96|P-value:1.46E-5 ID:SGTC_1848|Compound:st056003|FD-Score:-3.61|P-value:6.77E-5 ID:SGTC_1988|Compound:st071872|FD-Score:4.23|P-value:3.60E-6 ID:SGTC_2350|Compound:9023895|FD-Score:-3.60|P-value:7.26E-5 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:-3.14|P-value:4.54E-4 ID:SGTC_2537|Compound:larixol|FD-Score:3.39|P-value:1.60E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:-3.03|P-value:6.86E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:2.93|P-value:9.50E-4 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:4.17|P-value:4.87E-6 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:-3.32|P-value:2.28E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:3.83|P-value:2.47E-5 ID:SGTC_2930|Compound:9013230|FD-Score:4.06|P-value:8.51E-6 ID:SGTC_2937|Compound:9044753|FD-Score:-4.19|P-value:4.87E-6 ID:SGTC_3224|Compound:9130643|FD-Score:4.40|P-value:1.53E-6 ID:SGTC_3319|Compound:9138450|FD-Score:-3.25|P-value:3.02E-4

Top fitness defect scores for YML113W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_690 glyoxal 7.04 20.3 µM 4.23E-14 34 9.40E-13
2 SGTC_863 0466-0191 4.79 129.0 µM 1.95E-7 cell wall 49 8.51E-7
3 SGTC_3224 9130643 4.40 49.5 µM 1.53E-6 RPP1 & pyrimidine depletion 14 5.38E-6
4 SGTC_1988 st071872 4.23 53.4 µM 3.60E-6 5 1.16E-5
5 SGTC_2634 4'-methoxyflavone 4.17 100.0 µM 4.87E-6 25 1.52E-5
6 SGTC_2930 9013230 4.06 14.9 µM 8.51E-6 heme biosynthesis & mitochondrial translocase 33 2.50E-5
7 SGTC_799 1310-0557 3.84 151.0 µM 2.32E-5 superoxide 27 6.13E-5
8 SGTC_2752 diclazuril 3.83 33.1 µM 2.47E-5 131 6.49E-5
9 SGTC_1226 3909-7979 3.77 35.4 µM 3.25E-5 46 8.31E-5
10 SGTC_2537 larixol 3.39 68.9 µM 1.60E-4 46 3.46E-4
11 SGTC_1512 0259-0544 3.33 12.2 µM 2.04E-4 46 4.31E-4
12 SGTC_277 nystatin 3.23 15.5 µM 3.00E-4 39 6.09E-4
13 SGTC_1376 2910-0861 3.20 41.3 µM 3.37E-4 50 6.77E-4
14 SGTC_1244 0416-0015 3.15 52.5 µM 4.12E-4 ERG2 80 8.10E-4
15 SGTC_312 4092-0653 3.02 6.8 µM 6.84E-4 ergosterol depletion effects on membrane 134 0.00128
16 SGTC_1566 6,7-dimethoxy-4-ethylcoumarin 2.97 85.4 µM 8.07E-4 74 0.00148
17 SGTC_2592 piperlongumine 2.93 83.3 µM 9.50E-4 TSC3-RPN4 82 0.00171
18 SGTC_1235 benzylparaben 2.92 21.5 µM 9.65E-4 55 0.00174
19 SGTC_1346 1488-2224 2.89 62.4 µM 0.00106 58 0.00190
20 SGTC_2648 atorvastatin 2.87 78.7 µM 0.00117 azole & statin 56 0.00207

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.196 2.07E-30 YGR190C_d YGR190C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W
0.169 7.60E-23 YNR031C SSK2 hom MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress
0.168 9.04E-23 YNL307C MCK1 hom Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family
0.165 8.51E-22 YGR077C PEX8 hom Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.137 1.89E-15 YPR074C TKL1 hom Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication
0.134 7.69E-15 YLR113W HOG1 hom Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress
0.133 1.10E-14 YNL229C URE2 hom Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion
0.129 6.26E-14 YKL213C DOA1 hom WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress
0.129 6.23E-14 YOR364W_d YOR364W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C
0.119 5.57E-12 YDL080C THI3 hom Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis
0.115 2.46E-11 YNL135C FPR1 hom Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function
0.113 5.68E-11 YJL110C GZF3 hom GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication
0.108 3.49E-10 YOR225W_d YOR225W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.108 3.98E-10 YKL036C_d YKL036C_d het Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W
0.107 4.49E-10 YML117W NAB6 hom Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP