HIPHOP chemogenomics database

YML117W / NAB6

Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP

Zygosity: Homozygous strain

fixedexpanded
Profile for YML117W / NAB6

Click on Significant Values for Screen Details

Top fitness defect scores for YML117W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_58	0957-0393	13.80	9.0 µM	7.89E-45	cell wall	35	1.03E-43
2	SGTC_138	0850-0259	12.30	40.9 µM	7.80E-36	cell wall	26	5.98E-35
3	SGTC_2619	obliquin	10.70	87.9 µM	6.80E-28	cell wall	18	3.25E-27
4	SGTC_2367	9070478	10.20	135.4 µM	2.97E-25	cell wall	2	1.21E-24
5	SGTC_453	enantio-paf c16	10.10	10.0 µM	4.37E-25		6	1.77E-24
6	SGTC_514	4466-0038	10.10	2.6 nM	9.74E-25	cell wall	21	3.86E-24
7	SGTC_690	glyoxal	9.19	20.3 µM	6.30E-21		20	1.99E-20
8	SGTC_3351	9150312	9.13	21.6 µM	1.09E-20	cell wall	2	3.40E-20
9	SGTC_2316	5844804	8.96	197.4 µM	5.50E-20		1	1.64E-19
10	SGTC_916	4466-0038	8.58	42.0 nM	1.69E-18	cell wall	10	4.63E-18
11	SGTC_2584	androsterone	7.14	48.7 µM	2.31E-13	cell wall	35	4.67E-13
12	SGTC_467	ro 31-8220	6.86	72.8 µM	1.84E-12	calcium & mitochondrial duress	25	3.52E-12
13	SGTC_542	3381-0244	6.60	89.3 µM	1.15E-11		13	2.09E-11
14	SGTC_397	arbidol	5.87	50.0 µM	1.38E-9	cell wall	2	2.24E-9
15	SGTC_1282	0886-0010	5.47	32.4 µM	1.50E-8	endomembrane recycling	14	2.28E-8
16	SGTC_2582	agaric acid	5.24	100.0 µM	5.38E-8	redox potentiating	210	7.91E-8
17	SGTC_2848	9016370	5.20	26.0 µM	6.98E-8		8	1.02E-7
18	SGTC_666	0272-0154	5.02	136.0 µM	1.83E-7	cell wall	31	2.61E-7
19	SGTC_3350	9150499	4.84	3.6 µM	4.60E-7	cell wall	28	6.40E-7
20	SGTC_379	3970-0972	4.54	5.9 µM	2.12E-6	tubulin folding & SWR complex	20	2.85E-6

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.496	9.11E-208	YMR124W_p	YMR124W_p	hom	Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.456	1.90E-171	YJL159W	HSP150	hom	O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.449	5.34E-166	YJR054W	KCH1	hom	Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.448	2.06E-165	YJR142W_p	YJR142W_p	hom	Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.394	2.19E-124	YLL032C	YLL032C	hom	Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.371	1.27E-109	YOL003C	PFA4	hom	Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.359	1.12E-102	YJR111C_p	YJR111C_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria
0.356	2.28E-100	YDL021W	GPM2	hom	Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event
0.355	5.32E-100	YLL021W	SPA2	hom	Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate
0.353	1.32E-98	YMR121C	RPL15B	hom	Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
0.348	6.29E-96	YBL006C	LDB7	hom	Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions
0.344	1.45E-93	YDL203C	ACK1	hom	Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.339	4.36E-91	YOR051C	ETT1	hom	Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough
0.332	4.42E-87	YBR020W	GAL1	hom	Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication
0.332	5.87E-87	YKL033W-A_p	YKL033W-A_p	hom	Putative protein of unknown function; similar to uncharacterized proteins from other fungi