YML125C / PGA3

Putative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of lifespan; required for maturation of Gas1p and Pho8p, proposed to be involved in protein trafficking; PGA3 has a paralog, AIM33, that arose from the whole genome duplication

Zygosity: Heterozygous strain
fixedexpanded
Profile for YML125C / PGA3

Click on Significant Values for Screen Details ID:SGTC_67|Compound:0335-0847|FD-Score:3.21|P-value:1.70E-4 ID:SGTC_220|Compound:paclitaxel|FD-Score:-2.79|P-value:8.53E-4 ID:SGTC_534|Compound:k292-0788|FD-Score:3.17|P-value:1.98E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:-2.79|P-value:8.39E-4 ID:SGTC_835|Compound:0312-0010|FD-Score:-3.97|P-value:3.97E-6 ID:SGTC_844|Compound:0330-0132|FD-Score:3.23|P-value:1.52E-4 ID:SGTC_911|Compound:3456-4043|FD-Score:2.98|P-value:4.34E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:-3.50|P-value:4.15E-5 ID:SGTC_1022|Compound:4477-0945|FD-Score:3.90|P-value:6.55E-6 ID:SGTC_1037|Compound:k284-1091|FD-Score:-2.77|P-value:9.01E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:3.49|P-value:4.82E-5 ID:SGTC_1247|Compound:0448-0043|FD-Score:3.57|P-value:3.35E-5 ID:SGTC_1269|Compound:0831-0796|FD-Score:2.95|P-value:4.91E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:3.23|P-value:1.55E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.21|P-value:1.56E-4 ID:SGTC_1554|Compound:st069299|FD-Score:3.28|P-value:1.22E-4 ID:SGTC_1588|Compound:farnesol|FD-Score:-2.98|P-value:3.94E-4 ID:SGTC_1705|Compound:st027868|FD-Score:3.49|P-value:4.92E-5 ID:SGTC_1708|Compound:st033234|FD-Score:4.44|P-value:3.40E-7 ID:SGTC_1719|Compound:st032227|FD-Score:-3.34|P-value:8.56E-5 ID:SGTC_1831|Compound:st055328|FD-Score:2.85|P-value:7.34E-4 ID:SGTC_1916|Compound:st067606|FD-Score:2.85|P-value:7.46E-4 ID:SGTC_1933|Compound:st074711|FD-Score:3.28|P-value:1.27E-4 ID:SGTC_2015|Compound:5119600|FD-Score:3.19|P-value:1.87E-4 ID:SGTC_2019|Compound:4016004|FD-Score:-3.45|P-value:5.19E-5 ID:SGTC_2020|Compound:4017018|FD-Score:-2.98|P-value:3.98E-4 ID:SGTC_2052|Compound:5265182|FD-Score:3.07|P-value:3.10E-4 ID:SGTC_2261|Compound:9010538|FD-Score:-3.02|P-value:3.45E-4 ID:SGTC_2283|Compound:7944793|FD-Score:3.45|P-value:5.86E-5 ID:SGTC_2285|Compound:7945142|FD-Score:3.06|P-value:3.23E-4 ID:SGTC_2288|Compound:7946289|FD-Score:4.61|P-value:1.27E-7 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:-2.88|P-value:5.99E-4 ID:SGTC_2786|Compound:7743617|FD-Score:-3.31|P-value:9.66E-5 ID:SGTC_2793|Compound:7325131|FD-Score:-3.38|P-value:7.15E-5 ID:SGTC_2932|Compound:9016711|FD-Score:4.69|P-value:7.80E-8 ID:SGTC_3003|Compound:9075612|FD-Score:3.00|P-value:4.07E-4 ID:SGTC_3007|Compound:9078383|FD-Score:-2.79|P-value:8.41E-4 ID:SGTC_3030|Compound:9087619|FD-Score:3.30|P-value:1.14E-4 ID:SGTC_3180|Compound:9109287|FD-Score:-2.76|P-value:9.53E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:3.21|P-value:1.70E-4 ID:SGTC_220|Compound:paclitaxel|FD-Score:-2.79|P-value:8.53E-4 ID:SGTC_534|Compound:k292-0788|FD-Score:3.17|P-value:1.98E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:-2.79|P-value:8.39E-4 ID:SGTC_835|Compound:0312-0010|FD-Score:-3.97|P-value:3.97E-6 ID:SGTC_844|Compound:0330-0132|FD-Score:3.23|P-value:1.52E-4 ID:SGTC_911|Compound:3456-4043|FD-Score:2.98|P-value:4.34E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:-3.50|P-value:4.15E-5 ID:SGTC_1022|Compound:4477-0945|FD-Score:3.90|P-value:6.55E-6 ID:SGTC_1037|Compound:k284-1091|FD-Score:-2.77|P-value:9.01E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:3.49|P-value:4.82E-5 ID:SGTC_1247|Compound:0448-0043|FD-Score:3.57|P-value:3.35E-5 ID:SGTC_1269|Compound:0831-0796|FD-Score:2.95|P-value:4.91E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:3.23|P-value:1.55E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.21|P-value:1.56E-4 ID:SGTC_1554|Compound:st069299|FD-Score:3.28|P-value:1.22E-4 ID:SGTC_1588|Compound:farnesol|FD-Score:-2.98|P-value:3.94E-4 ID:SGTC_1705|Compound:st027868|FD-Score:3.49|P-value:4.92E-5 ID:SGTC_1708|Compound:st033234|FD-Score:4.44|P-value:3.40E-7 ID:SGTC_1719|Compound:st032227|FD-Score:-3.34|P-value:8.56E-5 ID:SGTC_1831|Compound:st055328|FD-Score:2.85|P-value:7.34E-4 ID:SGTC_1916|Compound:st067606|FD-Score:2.85|P-value:7.46E-4 ID:SGTC_1933|Compound:st074711|FD-Score:3.28|P-value:1.27E-4 ID:SGTC_2015|Compound:5119600|FD-Score:3.19|P-value:1.87E-4 ID:SGTC_2019|Compound:4016004|FD-Score:-3.45|P-value:5.19E-5 ID:SGTC_2020|Compound:4017018|FD-Score:-2.98|P-value:3.98E-4 ID:SGTC_2052|Compound:5265182|FD-Score:3.07|P-value:3.10E-4 ID:SGTC_2261|Compound:9010538|FD-Score:-3.02|P-value:3.45E-4 ID:SGTC_2283|Compound:7944793|FD-Score:3.45|P-value:5.86E-5 ID:SGTC_2285|Compound:7945142|FD-Score:3.06|P-value:3.23E-4 ID:SGTC_2288|Compound:7946289|FD-Score:4.61|P-value:1.27E-7 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:-2.88|P-value:5.99E-4 ID:SGTC_2786|Compound:7743617|FD-Score:-3.31|P-value:9.66E-5 ID:SGTC_2793|Compound:7325131|FD-Score:-3.38|P-value:7.15E-5 ID:SGTC_2932|Compound:9016711|FD-Score:4.69|P-value:7.80E-8 ID:SGTC_3003|Compound:9075612|FD-Score:3.00|P-value:4.07E-4 ID:SGTC_3007|Compound:9078383|FD-Score:-2.79|P-value:8.41E-4 ID:SGTC_3030|Compound:9087619|FD-Score:3.30|P-value:1.14E-4 ID:SGTC_3180|Compound:9109287|FD-Score:-2.76|P-value:9.53E-4

Top fitness defect scores for YML125C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2932 9016711 4.69 44.5 µM 7.80E-8 5 1.36E-6 0.06
2 SGTC_2288 7946289 4.61 167.3 µM 1.27E-7 4 2.00E-6 0.66
3 SGTC_1708 st033234 4.44 31.8 µM 3.40E-7 2 4.44E-6 0.63
4 SGTC_1022 4477-0945 3.90 21.3 µM 6.55E-6 6 4.80E-5 0.20
5 SGTC_1247 0448-0043 3.57 5.8 µM 3.35E-5 ERG2 16 1.80E-4 0.19
6 SGTC_1243 0416-0003 3.49 5.7 µM 4.82E-5 PDR1 10 2.41E-4 0.14
7 SGTC_1705 st027868 3.49 56.3 µM 4.92E-5 4 2.45E-4 0.16
8 SGTC_2283 7944793 3.45 200.0 µM 5.86E-5 10 2.82E-4 0.00
9 SGTC_3030 9087619 3.30 49.5 µM 1.14E-4 15 4.83E-4 0.17
10 SGTC_1554 st069299 3.28 38.7 µM 1.22E-4 7 5.12E-4 0.01
11 SGTC_1933 st074711 3.28 15.9 µM 1.27E-4 20 5.26E-4 0.11
12 SGTC_844 0330-0132 3.23 15.3 µM 1.52E-4 6 6.09E-4 0.16
13 SGTC_1378 2093-0007 3.23 380.0 nM 1.55E-4 14 6.19E-4 0.01
14 SGTC_67 0335-0847 3.21 266.0 µM 1.70E-4 ubiquinone biosynthesis & proteasome 35 6.68E-4 0.02
15 SGTC_2015 5119600 3.19 184.0 µM 1.87E-4 17 7.20E-4 0.05
16 SGTC_534 k292-0788 3.17 63.4 µM 1.98E-4 mitochondrial processes 18 7.56E-4 0.00
17 SGTC_2052 5265182 3.07 7.1 µM 3.10E-4 18 0.00109 0.03
18 SGTC_2285 7945142 3.06 200.0 µM 3.23E-4 20 0.00112 0.05
19 SGTC_3003 9075612 3.00 71.4 µM 4.07E-4 14 0.00135 0.12
20 SGTC_911 3456-4043 2.98 236.0 µM 4.34E-4 mitochondrial processes 27 0.00143 0.11

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.154 3.22E-19 YEL004W YEA4 hom Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER
0.135 4.30E-15 YKL166C TPK3 hom cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk2p; localizes to P-bodies during stationary phase; TPK3 has a paralog, TPK1, that arose from the whole genome duplication
0.117 1.27E-11 YKR003W OSH6 hom Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion
0.112 6.70E-11 YFR042W KEG1 het Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability
0.108 3.59E-10 YDR392W SPT3 hom Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
0.103 2.27E-9 YOR152C_p YOR152C_p hom Putative protein of unknown function; YOR152C is not an essential gene
0.098 1.08E-8 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.098 1.48E-8 YMR110C HFD1 hom Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder
0.097 1.83E-8 YMR107W SPG4 hom Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources
0.096 2.48E-8 YGL067W NPY1 hom NADH diphosphatase (pyrophosphatase), hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes; nudix hydrolase family member
0.095 3.49E-8 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.094 4.74E-8 YCR015C_p YCR015C_p hom Putative protein of unknown function; YCR015C is not an essential gene
0.094 4.80E-8 YMR322C_p SNO4_p hom Possible chaperone and cysteine protease, similar to bacterial Hsp31 and yeast Hsp31p, Hsp32p, and Hsp33p; DJ-1/ThiJ/PfpI superfamily member; predicted involvement in pyridoxine metabolism; induced by mild heat stress and copper deprivation
0.093 6.21E-8 YJL055W_p YJL055W_p hom Putative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU
0.093 7.39E-8 YGR292W MAL12 hom Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose